고정화 시스템을 이용한 용균효소의 생산

류병호,박종옥,진성현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

해양에서 분리된 용균활성이 우수한 균주의 균체고정화에 의한 연속생산을 위한 조건을 검토하였다. Bacillus subtilis SH-1의 균체를 고정화하여 column형 reactor에 의하여 연속적으로 효소생산을 실험하였다. 연속발효의 최적조건은 담체는 3.0% sodium alginate로 직경 2.4mm의 bead를 제조하여 기질의 주입속도는 20ml/h 공기의 주입속도는 2.0ml/min이였다. Column형 reactor(용량 2.8ℓ)에 의한 연속발효시 25일까지 76.5~88.0 units/ml의 최적의 효소활성을 갖는 용균효소를 생산할 수 있었다. Bacillus subtilis SH-1 screened from coastal sea water of South Korea was used to produce bacteriolytic enzyme. The production of bacteriolytic enzyme by immobilized cells was investigated. The optimum conditions for the continuous production of the bacteriolytic enzyme using immobilized cells were 2.4 mm diameter of 0.3% alginate beads, 20 ml/h of substrate feeding rate and 20 ℓ/min of aeration rate. A productivity of 76.5 to 88.0 units/ml could be obtained for 25 days by continuous column reactor under the optimum conditions.

상악골에 발생한 악성섬유성조직구종의 치험례

이상철,김여갑,류동목,이백수,윤옥병,신민철 大韓顎顔面成形再建外科學會 1998 Maxillofacial Plastic Reconstructive Surgery Vol.20 No.1

상아질접착제에 대한 광조사가 접착에 미치는 영향

류현옥,김기옥,김성교 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

The purpose of this study was to investigate the influence of light irradiation over self-priming adhesive on dentin bonding. After acid etching the exposed dentin, a self-priming adhesive (Prime&Bond□NT dental adhesive system, Dentsply DeTrey, GmbH, Konstanz, Germany) was applied and light irradiation was done for 20 sec with regular intensity (600 mW/㎠) in group I and for 3 sec with ultra-high intensity (1930 mW/㎠) in group III. No light irradiation was done over self-priming adhesive in groups II and IV. Composite resin was added on the self-priming adhesive and irradiated for 40 sec with regular intensity (600 mW/㎠) in groups I and II and for 3 sec with ultra-high intensity (1930 mW/㎠) in groups III and IV. To see the effect of light curing time on dentin bonding, another 3 group specimens were prepared. Without light-irradiation over self-priming adhesive, added composite resin was irradiated for 3, 6, or 12 sec with ultra-high intensity light. After bonded specimens were stored in 37℃ distilled water for 24 hours, shear bond strength were measured using a universal testing machine (4202, Instron, Instron Co., U.S.A.) and fractured surfaces were examined under a stereomicroscope (SZ-PT Olympus, Japan) . Statistical analysis were done with one-way, two-way ANOVA and chi-square test. The results were as follows: 1.The shear bond strengths from the groups irradiated over self-priming adhesive were significantly higher than those from the groups without irradiation (p<0.05). 2.There was no significant shear bond strength difference between regular intensity light irradiation groups and ultra-high intensity ones (p>0.05). 3. There was no significant shear bond strength difference among various irradiation time groups with ultra-high intensity ones (p>0.05). 4.In stereomicroscopic examination of fractured surfaces, adhesive-cohesive mixed failure mode was mostly seen in all groups, and there was no significant difference in failure mode among groups (p>0.05) . Key words : Light irradiation, Self-priming adhesive, Dentin bonding, Shear bond strength

담도 및 췌장 악성종양에서의 CAl9-9의 진단적 가치에 대한 평가

명승재,최승목,김은옥,최호순,이성구,김명환,민영일,류진숙 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.3

감염 조절용 차단막의 두께가 광중합기의 중합광에 미치는 영향

장훈상,이석련,홍성옥,류현욱,송창규,민경산 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.5

연구목적: 본 연구는 감염 조절용 차단막을 여러 겹으로 사용했을 때 광중합기의 광강도와 파장, light diffusion 등에 미치는 영향에 대해 조사하였다. 연구 재료 및 방법: 감염 조절용 차단막은 투명 랩 (크린랩)을 사용하였고 광중합기는 할로겐 광중합기 (Optilux 360)와 LED 광중합기 (Elipar FreeLight 2)를 사용하였다. 차단막을 1겹, 2겹, 4겹, 8겹으로 광중합기의 광섬유말단을 감싸고 휴대용 광강도 측정기 (Cure Rite)로 광중합기의 광강도를 측정하였다. 광중합기를 주문제작한 optical breadboard에 고정시킨 후 휴대용 spectroradiometer (CS-1000)를 이용하여 광중합기의 파장을 측정하였고, DSLR (Nikon D70s)을 이용하여 광중합기의 light diffusion을 사진 촬영하였다. 결과: 광강도 측정 결과는 차단막의 두께가 증가할수록 광강도가 유의하게 감소하였으나 할로겐 광중합기에서 1겹과 2겹 사이에는 유의차가 없었으며, 4겹 이상의 차단막을 투과할 때 광강도가 더 많이 감소하였다. 여러 겹의 차단막을 투과한 광중합기의 전체적인 파장 형태와 peak wavelength의 변화는 관찰되지 않았다. Light diffusion 사진 촬영 시, LED 광중합기에서는 차단막의 두께가 미치는 영향이 없었으나 할로겐 광중합기에서는 차단막을 4겹 사용했을 때부터 중합광이 조사되는 각도가 감소하기 시작하여 8겹 사용했을 때 통계적으로 유의하게 감소하는 것을 볼 수 있었다 (p < 0.05). 결론: 광중합형 복합레진을 광중합할 경우 감염 조절용 차단막이 찢어지는 경우를 대비하여 1겹으로 사용하기 보다는 2겹으로 사용하는 것이 환자간의 교차감염을 예방하는데 유리할 것으로 사료된다. Objectives: This study investigated the effect of infection control barrier thickness on power density, wavelength, and light diffusion of light curing units. Materials and Methods: Infection control barrier (Cleanwrap) in one-fold, two-fold, four-fold, and eightfold, and a halogen light curing unit (Optilux 360) and a light emitting diode (LED) light curing unit (Elipar FreeLight 2) were used in this study. Power density of light curing units with infection control barriers covering the fiberoptic bundle was measured with a hand held dental radiometer (Cure Rite). Wavelength of light curing units fixed on a custom made optical breadboard was measured with a portable spectroradiometer (CS-1000). Light diffusion of light curing units was photographed with DSLR (Nikon D70s) as above. Results: Power density decreased significantly as the layer thickness of the infection control barrier increased, except the one-fold and two-fold in halogen light curing unit. Especially, when the barrier was four-fold and more in the halogen light curing unit, the decrease of power density was more prominent. The wavelength of light curing units was not affected by the barriers and almost no change was detected in the peak wavelength. Light diffusion of LED light curing unit was not affected by barriers, however, halogen light curing unit showed decrease in light diffusion angle when the barrier was four-fold and statistically different decrease when the barrier was eight-fold (p < 0.05). Conclusions: It could be assumed that the infection control barriers should be used as two-fold rather than one-fold to prevent tearing of the barriers and subsequent cross contamination between the patients.

요로결석 환자의 체외충격파쇄석술 치료경과와 결석 특성과의 관련성

김성길,김기순,류소연,한미아,이선옥,노준 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.33 No.S

요로 결석에 대한 비 침습적 치료 방법인 체외충격파쇄석술에 의한 치료경과와 결석 특성과의 관련성을 파악키 위해 2002년 12월부터 2008년 3월까지 G시 H병원에서 요로 결석으로 진단되어 체외충격파쇄석술을 받은 33l례를 대상으로 의무기록 자료를 이용하여 분석한 결과는 다음과 같다. 1. 조사 대상자 331례중 남자는 68.6%, 여자는 31.4%이었으며 평균연령(표준편차)은 44.2 (13.9)세 이었고 84%가 G시내 거주자였다. 2. 조사 대상자에게 시행된 체외충격파쇄석술의 평균 시술횟수(표준편차)는 1.9(1.5)회 이었으며 평균 통증제거기간(표준편차)은 3.2(2.1)일 이었고 요로결석 진단 시로부터 치료가 완료되었다고 의사가 판정했을 때까지의 치료기간의 평균(표준편차)은 10.3(8.4)일 이었다. 3. 시술횟수에 통계적으로 유의하게(P<0.01) 관련된 요인은 결석크기, 결석의 방사선 투과성, 요관 폐색여부 이었으며 모형의 설명력은 13.5%이었다. 4. 통증제거기간에 통계적으로 유의하게(P<0.01) 관련된 요인은 결석의 방사선 투과성 이었으며 모형의 설명력은 7.4%이었다. 5. 치료기간에 통계적으로 유의하게(P<0.01) 관련된 요인은 결석크기와 결석의 방사선 투과성 이었으며 모형의 설명력은 13.5%이었다. 따라서 결석의 크기가 크거나 결석이 방사선에 비 투과성일 때, 요관이 폐색되어 있을 때 치료경과가 늦게 됨으로 이러한 사실을 참고 하여 추후 진료지침을 작성 시 보다 효과적이고 효율적인 치료법을 적용할 필요가 있다고 판단되며 보다 체계적인 평가와 연구가 필요할 것이다. Objectives & Methods: To find the treatment process by extracorporeal shock wave lithotripsy (ESWL) and its related stone characteristics in urinary tract stone patients, an analysis was done from medical records of 331 cases who were treated by ESWL from December 2002 to March 2008 at H hospital in G city. Results: Among 331 study subjects 68.6% were male and 31.4% were female. Their mean age (standard deviation, SD) was 44.2 (13.9) years old and 84.0% lived in G city. Average frequency of ESWL done toward study cases were 1.9±1.5 times. Mean duration of disappearance from pain was 3.2±2.1 days and total treatment period for a stone was 10.3±8.4 days. Statistically significant (p<0.01) stone and urinary tract characteristics related with frequency of ESWL were stone size, radio-opacity of the stone, and obstruction of urinary tract. The model composed of these 3 independent variables explained 13.5% of frequency of ESWL. Statistically significant (p<0.01) variable related with disappearance from pain was radio-opacity of the stone, and this model explained 7.4%. Statistically significant (p<0.01) variables related with treatment duration due to stone were stone size and radio-opacity of the stone. These two variables explained 13.5%. Conclusion: The bigger the size of stone are, the slower the treatment process will be. And when the stone is radio-opaque and urinary tract is obstructed, the treatment process will be slower than stone is radio-luscent and urinary tract is not obstructed. So to make effective and efficient treatment manual for ESWL, above fact must be considered and continuous systematic evaluation and research toward ESWL are necessary.

뇌졸중 후 치매환자에서 도네피질(아리셉트^(?))이 인지기능 및 일상생활 수행능력, 삶의 질에 미치는 효과

이상열,장혁,송재은,조성옥,류정미,이미경,조진호,신용일 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.1

Background: This study evaluated the efficacy of administering Donepezil(Aricept^(?)) for improving cognitive function, activities of daily living, and quality of life in patients with post-stroke dementia. Methods: Patients(n=99; mean age, 69.89 years; 48.5% men) with post-stroke dementia, according to criteria of the DSM-IV and MRI, were treated with donepezil 5mg/day or 10mg/day during 24 weeks. They were examined using Mini-mental Status Examination(MMSE), Chinical Dementia Rating(CDR), Barthel Activities of daily living(B-ADL), Instrumental Activities of daily living(I-ADL), Short Form 36 Health Survey-Korean(SF-36-K), and Geriatric Depression Scale(GDS) at before treatment and 24 weeks after treatment. Results: The 97 patients completed the trial. At 24 weeks, they showed significant improvement in MMSE(p<0.001), CDR(p<0.001), B-ADL(p=0.002), I-ADL(p<0.001), and SF-36-K(p=0.003) except GDS(p=0.288). Donepezil was well tolerated. There was no withdrawal due to adverse events. Conclusion: This results demonstrate that donepezil was beneficial in improving the cognitive function, activities of daily living, and quality of life in patients with post-stroke dementia and was well toleraetd.

한국인에서 발생한 말라리아의 임상적 특징

김은옥,이미숙,이상오,이선화,김양수,우준희,지현숙,류지소 대한감염학회 1998 감염 Vol.30 No.5

목적: 1993년 이후 우리나라에서 말라리아 환자가 급격하게 증가하는 추세이며, 말라리아의 임상 경과가 환자의 유전적인 요인에 영향을 받는 것으로 알려져 있기 때문에 한국인에서 말라리아의 임상 양상 및 예후의 특징을 조사하고자 하였다. 방법: 1989년 6월부터 1997년 10월까지 서울중앙병원에 입원하여 말초 혈액 도말 검사로 말라리아가 확진된 환자를 대상으로 의무 기록을 후향적으로 검토하였다. 토착형 삼일열 말라리아와 열대열 말라리아 임상 양상 및 합병증, 예후를 비교하였고, 이를 종합한 한국인에서 말라리아의 임상상을 서양인에서의 meta-analysis 결과와 비교하였다. 결과: 전체 말라리아 환자는 28예로 매년 증가하는 추세를 보였으며, 삼일열 말라리아 17예, 열대열 말라리아 11예이었다. 토착형 말라리아 15예 모두가 삼일열 말라리아이었고, 이 중 9예는 전방 근무 후 제대한 지 1년 이내의 전역 군인이었다. 유입형 말라리아 13예 중 열대열 말라리아 11예이었고, 이중 8예가 아프리카 여행 후 발병하였다. 임상 양상을 보면 환자 모두에서 발열이 있었으며, 주기적인 발열은 토착형 삼일열 말라리아 환자 6예에서만 관찰할 수 있었다. 혈액학적 이상이 토착형 삼일열 말라리아와 열대열 말라리아 환자 모두에서 가장 흔한 검사 소견이었으며, 혈소판 감소, 빈혈, 백혈구 강의의 빈도는 양군 사이에 차이가 없었다. 열대열 말라리아에서는 의식 변화의 소견이 흔하였고 간효소, 빌리루빈과 혈청 크레아티닌이 상승된 경우가 더 많았으며 뇌말라리아, 급성 신부전 등의 합병증이 더 흔히 발생하였다. 본 연구에 포함된 환자와 서양인에서의 임상 양상을 비교하여 보면 한국인 환자에서 빈혈, 혈소판 감소, 백혈구 감소, 혈청 간효소치의 상승을 더 흔하게 관찰할 수 있었으나 황달, 뇌말라리아, 신부전 등 합병증에 있어서는 양군 사이에 차이가 없었다. 결론: 최근 말라리아 환자는 지속적으로 증가하고 있었으며, 한국인 말라리아 환자 모두 발열이 관찰되었고, 혈소판 감소, 빈혈, 백혈구 감소가 가장 흔한 검사 소견이었다. Background: Vivax malaria has been a reemerging disease since 1993 in Korea, and the number of cases are rapidly increasing. Clinical manifestations of malaria are affected by genetic factors and individual patient's characteristics. In this study, we evaluated clinical manifestations and prognosis of malaria in Korean patients. Methods: Medical records of the patients confirmed as malaria by peripheral blood smear in Asan Medical Center from June, 1989 to October, 1997 were reviewed retrospectively. The patients with vivax malaria and falciparum malaria were compared with respect to clinical manifestations, complications, and prognosis. We also compared our data to meta-analysis reports from western countries. Results: Twenty-eight cases of malaria were included. The number of cases increases by year. Seventeen of them were diagnosed as vivax malaria, and 11 as falciparum malaria. Fifteen indigenous malaria were all vivax malaria, 9 of which had finished the military service in the border between North and South Korea several months before the onset. Eleven of 13 imported malaria were falciparum malaria, 8 of which were acquired in Africa. All 28 patients had fever, but cyclic pattern was shown only in 6 patients of vivax malaria. Hematologic abnormalities were the most common laboratory findings, and there were no significant differences in the frequency of thrombocytopenia, anemia, and leukopenia between vivax malaria and falciparum malaria. Altered mentality, elevated transaminase, total bilirubin, and serum creatinine were more frequent in falciparum malaria than in vivax malaria. Korean patients showed anemia, thrombocytopenia, leukopenia, and elevation of transaminase more frequently than western patients. Conclusion: The number of malaria cases has been increasing by year. All Korean patients with malaria had fever, and the most common laboratory findings were thrombocytopenia, anemia, and leukopenia.

Eagle씨 증후군의 치험례

오승환,이상철,윤옥병,지유진,김여갑,류동목 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.3

Eagle's syndrome is the term given to symtomatic elogation of the styloid process or mineralization of the stylohyoid or stylomandibular ligament. Since ossification of stylohyoid ligament at cadeva was fist described by De Manchetics in 1652 and Weinlecher described clinical symptom which produced by elongated styloid process and osteotomy of styloid process in 1872, Clinical symptom which include sensation of a foreign body on the pharynx, dysphagai, dysphonia, referred pain, and mouth opening disturbance was termed by Eagle as Eagle's syndrome. then, case reports of Eagle's syndrome are presented. in these cases, the patient's chief complaints included periauricular radiating pain, mouth opening disturbance, foreign body sensation, dysphagia, tenderness on the neck. Through vairous X-ray examination and palpation of tonsillar fossa, elongted styloid process were confirmed. Under the general anesthesia there were successfully removed out via transoral approach technique, described by Eagle. after resented styloid process, they were freed from the symptoms without further complication. Therefor we reported these cases treated by surgical resection of styloid process with good results.

일차성 Streptococcus pneumoniae 균혈증에 동반된 흉부 하행 대동맥염 1예

전민혁,고옥배,정우진,최영준,이정훈,최상호,김미나,김양수,우준희,류지소 대한감염학회 2005 감염과 화학요법 Vol.37 No.5

페렴구균에 의한 대동맥염은 국내에서 1예만 보고될 정도로 매우 드문 감염증이다. 저자들은 원발성의 폐렴구균혈증에 합병된 페렴구균 대동맥염 환자 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Bacterial aortitis is rare and without surgical intervention, it usually leads to uncontrolled sepsis or catastrophic hemorrhage, ultimately, resulting in death. In the postantibiotic era, the dominant infecting organisms are Staphylococcus aureus and Salmonella spp.. In spite of frequent pneumococcal bacteremia, vascular seeding is rare and there is only one published report in Korea. We describe a 77-year-old female with primary pneumococcal bacteremia followed by aortitis of the descending aorta, which was successfully treated by aneurysmectomy, wide excision of periaortic tissue, aortobiiliac bypass via prosthetic graft, and antibiotic therapy.