Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells

Ruiping Zhang,Jing Li,Jianding Li,Jun Xie 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.9

Mesenchymal stem cells (MSCs) can differentiate into neural cells to treat nervous system diseases. Magnetic resonance is an ideal means for cell tracking through labeling cells with superparamagnetic iron oxide (SPIO). However, no studies have described the neural differentiation ability of SPIO-labeled MSCs, which is the foundation for cell therapy and cell tracking in vivo. Our results showed that bone marrow-derived mesenchymal stem cells (BM-MSCs) labeled in vitro with SPIO can be induced into neural-like cells without affecting the viability and labeling efficiency. The cellular uptake of SPIO was maintained after labeled BM-MSCs differentiated into neural-like cells, which were the basis for transplanted cells that can be dynamically and non-invasively tracked in vivo by MRI. Moreover, the SPIO-labeled induced neural-like cells showed neural cell morphology and expressed related markers such as NSE, MAP-2. Furthermore, whole-cell patch clamp recording demonstrated that these neural-like cells exhibited electrophysiological properties of neurons. More importantly, there was no significant difference in the cellular viability and [Ca2+]i between the induced labeled and unlabeled neural-like cells. In this study, we show for the first time that SPIO-labeled MSCs retained their differentiation capacity and could differentiate into neural-like cells with high cell viability and a good cellular state in vitro.

Optimization of photocatalytic degradation of Cefradine using a “green” goethite/H2O2 system

Ruiping Li,Shaoming Hong,Xiaocong Li,Bin Zhang,Hailin Tian,Yingping Huang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.78 No.-

An environmental friendly photocatalyst, goethite in the presence of H2O2, was used to remove Cefradinefrom aqueous solution. Four factors were examined using Box–Behnken Design and results wereanalyzed by response surface method. H2O2 concentration had the largest effect on Cefradine removaland the optimal reaction conditions were: H2O2 concentration, 4 mmol/L; solution pH, 5; goethitedosage, 1.2 g/L and illumination time, 9 h. Experimental data on Cefradine removal under optimalconditions closely coincided with model predictions, validating the model. Hydroxyl radicals ( OH) andsuperoxide anion were involved in the Cefradine photodegradation process and that OH makes thelarger contribution.

A rock physics simulator and its application for $CO_2$ sequestration process

Li, Ruiping,Dodds, Kevin,Siggins, A.F.,Urosevic, Milovan Korean Society of Earth and Exploration Geophysici 2006 지구물리와 물리탐사 Vol.9 No.1

Injection of $CO_2$ into underground saline formations, due to their large storage capacity, is probably the most promising approach for the reduction of $CO_2$ emissions into the atmosphere. $CO_2$ storage must be carefully planned and monitored to ensure that the $CO_2$ is safely retained in the formation for periods of at least thousands of years. Seismic methods, particularly for offshore reservoirs, are the primary tool for monitoring the injection process and distribution of $CO_2$ in the reservoir over time provided that reservoir properties are favourable. Seismic methods are equally essential for the characterisation of a potential trap, determining the reservoir properties, and estimating its capacity. Hence, an assessment of the change in seismic response to $CO_2$ storage needs to be carried out at a very early stage. This must be revisited at later stages, to assess potential changes in seismic response arising from changes in fluid properties or mineral composition that may arise from chemical interactions between the host rock and the $CO_2$. Thus, carefully structured modelling of the seismic response changes caused by injection of $CO_2$ into a reservoir over time helps in the design of a long-term monitoring program. For that purpose we have developed a Graphical User Interface (GUI) driven rock physics simulator, designed to model both short and long-term 4D seismic responses to injected $CO_2$. The application incorporates $CO_2$ phase changes, local pressure and temperature changes. chemical reactions and mineral precipitation. By incorporating anisotropic Gassmann equations into the simulator, the seismic response of faults and fractures reactivated by $CO_2$ can also be predicted. We show field examples (potential $CO_2$ sequestration sites offshore and onshore) where we have tested our rock physics simulator. 4D seismic responses are modelled to help design the monitoring program.

Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells

Zhang, Ruiping,Li, Jing,Li, Jianding,Xie, Jun Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.9

Mesenchymal stem cells (MSCs) can differentiate into neural cells to treat nervous system diseases. Magnetic resonance is an ideal means for cell tracking through labeling cells with superparamagnetic iron oxide (SPIO). However, no studies have described the neural differentiation ability of SPIO-labeled MSCs, which is the foundation for cell therapy and cell tracking in vivo. Our results showed that bone marrow-derived mesenchymal stem cells (BM-MSCs) labeled in vitro with SPIO can be induced into neural-like cells without affecting the viability and labeling efficiency. The cellular uptake of SPIO was maintained after labeled BM-MSCs differentiated into neural-like cells, which were the basis for transplanted cells that can be dynamically and non-invasively tracked in vivo by MRI. Moreover, the SPIO-labeled induced neural-like cells showed neural cell morphology and expressed related markers such as NSE, MAP-2. Furthermore, whole-cell patch clamp recording demonstrated that these neural-like cells exhibited electrophysiological properties of neurons. More importantly, there was no significant difference in the cellular viability and $[Ca^{2+}]_i$ between the induced labeled and unlabeled neural-like cells. In this study, we show for the first time that SPIO-labeled MSCs retained their differentiation capacity and could differentiate into neural-like cells with high cell viability and a good cellular state in vitro.

Influence of water injection parameters on the performance of a water-lubricated single-screw air compressor

Yanan Li,Jingfu Wang,Yuting Wu,Biao Lei,Ruiping Zhi,Lili Shen 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1

A thermodynamic model of a water-lubricated single-screw air compressor was established to examine the impact of water injection parameters on performance. Heat transfer and leakage between humid air and water was considered by analyzing the impacts of rotation speed, discharge pressure, the rate of water injection, and diameter of droplets on the performance of a compressor. The discharge temperature could be reduced by increasing the rate of water injection, resulting in the compression procedure moving towards an isothermal state. The increase in the rate of water injection under rated conditions from 60 L/min to 80 L/min resulted in a reduction in the compressor discharge temperature, increased the volume efficiency, and increased adiabatic efficiency by 11.1 K, 1.5 %, and 2.8 %, respectively. Water injection atomization increased the area of transfer of heat between humid air and water and improved the performance of compressor.

Polyurethane Membrane with Temperature- and pH-Controllable Permeability for Amino-Acids

Hu Zhou,Ruiping Xun,Kejian Wu,Zhihua Zhou,Bin Yu,Youxin Tang,Ning Li 한국고분자학회 2015 Macromolecular Research Vol.23 No.1

This work was focused on the investigation of the temperature- and pH-responsive polyurethane (PU)membranes and their permeability to amino-acids in response to environmental stimuli. The PU membrane wasprepared from a wet phase inversion method and a two-step solution polymerization from polycaprolactone diols(PCL), 4,4′-diphenylmethane diisocyanate (MDI), dimethylol propionic acid (DMPA), etc. The chemical structure,phase state, morphology and surface wettability of the membrane were characterized with Fourier transform infrared(FTIR) spectrometer, differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and contactangle tester, respectively. The temperature and pH responses of the membrane were investigated by means of anamino-acid permeate experiment. The L-phenylalanine (L-Phe) was chosen as model amino-acids. The permeation of theL-Phe was measured using a dead-end flow filtration at varied temperatures and pH, and characterized by the permeateflux (J) and rejection coefficient (R). J of the L-Phe across the PU membrane increased with increasingtemperature and showed a sharp increase when temperature was raised to the crystalline melting temperature (Tm)of the soft segment of PU, while decreased with increasing pH and having a sharp decrease when pH reached thedissociation constant (pKa) of DMPA contained in PU macromolecules. While, the R behavior of L-Phe was justopposite from the results of J, which decreased with increasing temperature and increased with increasing pH,also showing the temperature and pH responses. Hopefully, the PU membrane with temperature- and pH-controllablepermeability has promising prospects in water treatment, membrane separation, drug delivery system, etc.

The Protective Roles of S-adenosylmethionine Decarboxylase (SAMDC) Gene in Melon Resistance to Powdery Mildew Infection

Changming Liu,Xiaoling Li,Ruiping Yang,Yanling Mo,Yongqi Wang,Feng Xian,Xian Zhang,Fei Wang 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.6

Powdery mildew caused by Podosphaera xanthii (P. xanthii) is one of important diseases in melon. Wehave previously investigated the differential gene expression in the incompatible P. xanthii-melon interactions andidentified one EST containing homologous sequences to S-adenosylmethionine decarboxylase (SAMDC) cDNA. Giventhis, SAMDC gene of Cucumis melo was cloned and designated as CmSAMDC in this study. It was 1,095 bp longand encoded a 364-amino acid peptide with a molecular mass of 40 kD. By sequence analyzing, the deducedCmSAMDC protein was shown to have two conserved regions of a putative proenzyme cleavage site and a PESTdomain. In addition, the expressions of CmSAMDC in the resistant melon materials increased more sharply than inthe susceptible the melon materials, and the higher polyamines (PAs) and hydrogen peroxide (H2O2) contents inresistant melon materials were found as well, which were accompanied by up-regulation of the stress-responsive defenseenzyme activities. Over-expression of CmSAMDC in Arabidopsis resulted in greatly reduced pathogen infection in theinoculated leaves of transgenic lines, enhanced resistance to powdery mildew, and the enhanced resistance appearedto be associated with pathogen-induced cell death. Taken together, our results suggested that CmSAMDC and perhapsits orthologous genes might be involved in responses of plants to biotrophic pathogens.

3D-monoclinic M–BTC MOF (M = Mn, Co, Ni) as highly efficient catalysts for chemical fixation of CO2 into cyclic carbonates

Yuanfeng Wu,Xianghai Song,Shuai Li,Jiahui Zhang,Xinghui Yang,Pengxin Shen,Lijing Gao,Ruiping Wei,Jin Zhang,Guomin Xiao 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-

[(CH3)2NH2][M3(BTC)(HCOO)4(H2O)].H2O (M–BTC, M = Mn, Ni, Co) were prepared under hydrothermal conditions and used as highly efficient catalysts for cycloaddition of CO2 with epichlorohydrin (ECH). The microstructure and physicochemical properties of the compounds were determined by PXRD, FT-IR, XPS, N2-adsorption, TG–DSC, NH3–TPD and CO2–TPD. 98.01% conversion of ECH and 96.05% selectivity to chloropropene carbonate was obtained over the Mn–BTC under the optimized reaction conditions (105 °C, 3.0 MPa, 9 h, 1.5 wt.% of ECH). Besides, the recyclability result exhibited the Mn–BTC compound can be utilized as least three times with a slight reduction in its catalytic ability. In addition, cycloaddition of CO2 with other epoxides and DFT calculation were also performed. The result exhibited the yield followed the order: ECH > 1, 2-epoxybutane > propene oxide > Allyl glycidyl ether, which was mainly determined by the energy of reaction.

Photocatalytic removal of cefazolin using Ag3PO4/BiOBr under visible light and optimization of parameters by response surface methodology

Yuhui Xiao,Xianghua Song,Zhuo Liu,Ruiping Li,Xiaorong Zhao,Yingping Huang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

The degradation of cefazolin (CFZ) by Ag3PO4/BiOBr composites under visible-light irradiation wasexplored. The main and interaction of parameters (catalyst dosage, pH, CFZ initial concentration anddegradation time) on removal of CFZ were studied by Box–Behnken design combined with responsesurface methodology. The pH was the most influential factor and both h+ and OH played a role in thephotocatalytic process. The high correlation coefficients (R2 = 0.9986 and adjusted R2 = 0.9973)demonstrated closefit between the predicted and experimental values. The exceptional efficiency ofAg3PO4/BiOBr composite in removing CFZ represents a promising technique for treatment of CFZcontainingwastewater.

Mn-Single-Atom Nano-multizyme Enabled NIR-II Photoacoustically Monitored, Photothermally Enhanced ROS Storm for Combined Cancer Therapy

Xiaozhe Wang,Xiaofeng Ren,Jie Yang,Zican Zhao,Xiaoyu Zhang,Fan Yang,Zheye Zhang,Peng Chen,Liping Li,Ruiping Zhang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Rationale To realize imaging-guided multi-modality cancer therapy with minimal side effects remains highly challenging. Methods We devised a bioinspired hollow nitrogen-doped carbon sphere anchored with individually dispersed Mn atoms (Mn/N-HCN) via oxidation polymerization with triton micelle as a soft template, followed by carbonization and annealing. Enzyme kinetic analysis and optical properties were performed to evaluate the imaging-guided photothermally synergized nanocatalytic therapy. Results Simultaneously mimicking several natural enzymes, namely peroxidase (POD), catalase (CAT), oxidase (OXD), and glutathione peroxidase (GPx), this nano-multizyme is able to produce highly cytotoxic hydroxyl radical (•OH) and singlet oxygen (1O2) without external energy input through parallel and series catalytic reactions and suppress the upregulated antioxidant (glutathione) in tumor. Furthermore, NIR-II absorbing Mn/N-HCN permits photothermal therapy (PTT), enhancement of CAT activity, and photoacoustic (PA) imaging to monitor the accumulation kinetics of the nanozyme and catalytic process in situ. Both in vitro and in vivo experiments demonstrate that near-infrared- II (NIR-II) PA-imaging guided, photothermally enhanced and synergized nanocatalytic therapy is efficient to induce apoptosis of cancerous cells and eradicate tumor tissue. Conclusions This study not only demonstrates a new method for effective cancer diagnosis and therapy but also provides new insights into designing multi-functional nanozymes.