Study on the internal irreversible losses and process exponent of single screw expanders

Lili Shen,Yuting Wu,Wei Wang,Biao Lei,Wei Duan,Ruiping Zhi 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.3

The irreversible losses including intake pressure, leakage, heat transfer, friction and over-expansion losses have great influence on the expander performance. In this paper, a thermodynamic model is presented to predict the real expansion process exponent and analyze the under-expansion or over-expansion under designed and off-designed operation conditions. The model verified by experimental results has a good agreement. Results showed that the real expansion process exponent of air is higher than the ideal adiabatic index of 1.4 and decreases from 1.716 to 1.644 with the internal volume ratio changing from 1.8 to 6.5. The real expansion process exponent of R123 is close to 1.00 under different internal volume ratio. Compared to the intake pressure, the variation of back pressure has greater influence on the large internal volume ratio than the small one. Thus, to adjust the back pressure is more effective to match the designed condition for the expander with a large internal volume ratio.

Cohesion Metrics for Evaluating Semantic Web Ontologies

Lili Liao,Guohua Shen,Zhiqiu Huang,Fei Wang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.11

With the widespread development of the Semantic Web, large-scale ontologies are being developed in more real-world applications to represent and integrate knowledge and data. There is an increasing need for measuring the cohesion of these ontologies for better understanding, maintenance, reuse and integration. The ontology cohesion metrics proposed in this paper can be used as a very useful complementarity of existing ontology cohesion metrics. Specifically, we first propose a set of evaluation metrics to measure the cohesion of ontologies based on directed acyclic graph. Following the framework for software measurement validation, we then evaluate the effectiveness of the proposed metrics theoretically. Finally, we conduct experiments using a set of classical ontologies; the results show that the proposed metrics are reasonable and effective.

Crocin alleviates neurotoxicity induced by bupivacaine in SH-SY5Y cells with inhibition of PI3K/AKT signaling

Lin Lili,Chen Zhen,Li Jun,Peng Jianye,Wang Jian,Feng Mingjun,Liu Tiancheng,Zhang Mengli,Wu Xian,Ai Fen,Shen Caijie 한국유전학회 2024 Genes & Genomics Vol.46 No.1

Background Bupivacaine, a common local anesthetic, can cause neurotoxicity and permanent neurological disorders. Crocin has been widely reported as a potential neuroprotective agent in neural injury models. Objective The aim of this study was to investigate the role and regulatory mechanism of crocin underlying bupivacaine-induced neurotoxicity. Method Human neuroblastoma SH-SY5Y cells were treated with bupivacaine and/or crocin for 24 h, followed by detecting cell viability using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effect of crocin or bupivacaine on SH-SY5Y cell proliferation was measured by Ki67 immunofluorescence assay. The levels of apoptosis-related proteins and the markers in the PI3K/Akt signaling pathway were examined using western blot analysis. The activities of caspase 3, catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were tested using respective commercial assay kits. Flow cytometry analysis was executed for detecting SH-SY5Y cell apoptosis. Result Crocin attenuated bupivacaine-induced neurotoxicity in SH-SY5Y cells. Meanwhile, crocin inhibited SH-SY5Y cell apoptosis induced by bupivacaine via repressing the activity of caspase-3, reducing Bax expression, and elevating Bcl-2 expression. Moreover, crocin mitigated oxidative stress in SH-SY5Y cells by increasing the content of CAT, SOD, GSH-Px and reducing the content of MDA. Additionally, crocin protected against bupivacaine-induced dephosphorylation of Akt and GSK-3β. The protective effects of crocin against bupivacaine-induced neurotoxicity in SH-SY5Y cells were counteracted by the Akt inhibitor. Conclusion These results suggested that crocin may exert a neuroprotective function by promoting cell proliferation and suppressing apoptosis and oxidative stress in SH-SY5Y cells. Thus, crocin might become a promising drug for the treatment of bupivacaine-induced neurotoxicity. Background Bupivacaine, a common local anesthetic, can cause neurotoxicity and permanent neurological disorders. Crocin has been widely reported as a potential neuroprotective agent in neural injury models. Objective The aim of this study was to investigate the role and regulatory mechanism of crocin underlying bupivacaine-induced neurotoxicity. Method Human neuroblastoma SH-SY5Y cells were treated with bupivacaine and/or crocin for 24 h, followed by detecting cell viability using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effect of crocin or bupivacaine on SH-SY5Y cell proliferation was measured by Ki67 immunofluorescence assay. The levels of apoptosis-related proteins and the markers in the PI3K/Akt signaling pathway were examined using western blot analysis. The activities of caspase 3, catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were tested using respective commercial assay kits. Flow cytometry analysis was executed for detecting SH-SY5Y cell apoptosis. Result Crocin attenuated bupivacaine-induced neurotoxicity in SH-SY5Y cells. Meanwhile, crocin inhibited SH-SY5Y cell apoptosis induced by bupivacaine via repressing the activity of caspase-3, reducing Bax expression, and elevating Bcl-2 expression. Moreover, crocin mitigated oxidative stress in SH-SY5Y cells by increasing the content of CAT, SOD, GSH-Px and reducing the content of MDA. Additionally, crocin protected against bupivacaine-induced dephosphorylation of Akt and GSK-3β. The protective effects of crocin against bupivacaine-induced neurotoxicity in SH-SY5Y cells were counteracted by the Akt inhibitor. Conclusion These results suggested that crocin may exert a neuroprotective function by promoting cell proliferation and suppressing apoptosis and oxidative stress in SH-SY5Y cells. Thus, crocin might become a promising drug for the treatment of bupivacaine-induced neurotoxicity.

Super-Resolution Reconstruction based on Tukey Norm and Adaptive Bilateral Total Variation

Jie Shen,Feng Xu,Mengxi Xu,Yun Yang,Ruili Wang,Lili Zhang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.5

In Bilateral Total Variation (BTV) regularized super-resolution reconstruction (SRR), the fidelity item is only applicable to a specific noise model, and the fixed weight of BTV regularization term cannot adapt to the changes in an image. Thus, this paper proposes a SRR algorithm based on the Tukey fidelity term and adaptive BTV regularization term. The Tukey fidelity term has a more effective outliers suppression feature to deal with complex noises, and the weight of adaptive BTV regularization term can resize itself according to the changes of image textures, which can achieve the purposes of suppressing noises and preserving edges. Experimental results show that, compared with other algorithms, the proposed algorithm has better vision effects and higher Peak Signal-to-noise Ratio (PSNR) values.

Water-soluble ginseng oligosaccharides protect against scopolamine-induced cognitive impairment by functioning as an antineuroinflammatory agent

Xu, Ting,Shen, Xiangfeng,Yu, Huali,Sun, Lili,Lin, Weihong,Zhang, Chunxiao The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.3

Background: Panax ginseng root is used in traditional oriental medicine for human health. Its main active components such as saponins and polysaccharides have been widely evaluated for treating diseases, but secondary active components such as oligosaccharides have been rarely studied. This study aimed to assess the impact of water-soluble ginseng oligosaccharides (WGOS), which were isolated from the warm-water extract of Panax ginseng root, on scopolamine-induced cognitive impairment in mice and its antineuroinflammatory mechanisms. Methods: We investigated the impact of WGOS on scopolamine-induced cognitive impairment in mice by using Morris water maze and novel object recognition task. We also analyzed the impact of WGOS on scopolamine-induced inflammatory response (e.g., the hyperexpression of proinflammatory cytokines IL-$1{\beta}$ and IL-6 and astrocyte activation) by quantitative real-time polymerase chain reaction and glial fibrillary acid protein (GFAP) immunohistochemical staining. Results: WGOS pretreatment protected against scopolamine-induced learning and memory deficits in the Morris water maze and in the novel object recognition task. Furthermore, WGOS pretreatment downregulated scopolamine-induced hyperexpression of proinflammatory cytokines interleukin (IL)-$1{\beta}$ and IL-6 mRNA and astrocyte activation in the hippocampus. These results indicate that WGOS can protect against scopolamine-induced alterations in learning and memory and inflammatory response. Conclusion: Our data suggest that WGOS may be beneficial as a medicine or functional food supplement to treat disorders with cognitive deficits and increased inflammation.

Single nucleotide polymorphism-based analysis of the genetic structure of Liangshan pig population

Liu, Bin,Shen, Linyuan,Guo, Zhixian,Gan, Mailing,Chen, Ying,Yang, Runling,Niu, Lili,Jiang, Dongmei,Zhong, Zhijun,Li, Xuewei,Zhang, Shunhua,Zhu, Li Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (P_N) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.

Effects of chronic alcohol exposure on ischemia–reperfusion-induced acute kidney injury in mice: the role of β-arrestin 2 and glycogen synthase kinase 3

Lihua Wang,Yifei Zhu,Lili Wang,Jingjing Hou,Yongning Gao,Lei Shen,Jingyu Zhang 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Little is known about the effects of chronic alcohol intake on the outcome of acute kidney injury (AKI). Hence, we examined the effects of chronic alcohol intake on the development of renal fibrosis following AKI in an animal model of bilateral renal ischemia–reperfusion (IR) injury. We first found that chronic alcohol exposure exacerbated bilateral IR-induced renal fibrosis and renal function impairment. This phenomenon was associated with increased bilateral IR-induced extracellular matrix deposition and an increased myofibroblast population as well as increased bilateral IR-induced expression of fibrosis-related genes in the kidneys. To explore the mechanisms underlying this phenomenon, we showed that chronic alcohol exposure enhanced β-arrestin 2 (Arrb2) expression and Akt and glycogen synthase kinase-3 (GSK3)β activation in the kidneys. Importantly, pharmacological GSK3 inhibition alleviated bilateral IR-induced renal fibrosis and renal function impairment. Furthermore, we demonstrated that Arrb2− / − mice exhibited resistance to IR-induced renal fibrosis and renal function impairment following chronic alcohol exposure, and these effects were associated with attenuated GSK3β activation in the kidneys. Taken together, our results suggest that chronic alcohol exposure may potentiate AKI via β-arrestin 2/Akt/GSK3β-mediated signaling in the kidney.

pH-Responsive Multifunctional Materials with Switchable Superamphiphobicity and Superoleophobicity-Superhydrophilicity for Controllable Oil/Water Separation

Mengnan Qu,Yichen Zhou,Lili Ma,Yi Zhang,Jiaxin Wang,Shanxin Xiong,Lihua Shen,Jinmei He 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3

Stimulus-responsive materials with controllable oil/water separation performance have prodigious potentialapplication. Here, a new thought for low-cost, time-saving, flexible approach has been developed to prepare a pH-responsivematerial with switchable superamphiphobicity and superoleophobicity-superhydrophilicity. The as-prepared material can beeasily applied onto multifarious substrates and presents stable superamphiphobicity. However, when the modified material istreated by alkaline solution, its surface wettability turns superhydrophilicity and superoleophobicity, thus water is allowed topenetrate through the material whereas the oil was blocked on the surface. Moreover, the surface wettability can be recoveredto superamphiphobicity quickly via treating the material with acidic aqueous. Hence, such a controllable water wettabilityand stable oil repellency property endows the as-prepared material with excellent capability to separate water from oil/watermixture. In addition, the pH-responsive materials can maintain switchable wettability after being treated by acid and alkalinumerous cycles. Furthermore, the obtained materials also exhibit excellent recyclable, self-cleaning and flame-resistantperformance, which shows potential applications for smart water-oil separators and fire-shielding protectors.

Biological Potential of Bioorganic Fertilizer Fortified with Bacterial Antagonist for the Control of Tomato Bacterial Wilt and the Promotion of Crop Yields

( Kai Wu ),( Zhiying Fang ),( Lili Wang ),( Saifei Yuan ),( Rong Guo ),( Biao Shen ),( Qirong Shen ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.10

The application of Bacillus sp. in the biological control of plant soilborne diseases has been shown to be an environmentally friendly alternative to the use of chemical fungicides. In this study, the effects of bioorganic fertilizer (BOF) fortified with Bacillus amyloliquefaciens SQY 162 on the suppression of tomato bacterial wilt were investigated in pot experiments. The disease incidence of tomato wilt after the application of BOF was 65.18% and 41.62% lower at 10 and 20 days after transplantation, respectively, than in the control condition. BOF also promoted the plant growth. The SQY 162 populations efficiently colonized the tomato rhizosphere, which directly suppressed the number of Ralstonia solanacearum in the tomato rhizosphere soil. In the presence of BOF, the activities of defense-related enzymes in tomato were lower than in the presence of the control treatment, but the expression levels of the defense-related genes of the plants in the salicylic acid and jasmonic acid pathways were enhanced. It was also found that strain SQY 162 could secrete antibiotic surfactin, but not volatile organic compounds, to suppress Ralstonia. The strain could also produce plant growth promotion compounds such as siderophores and indole-3-acetic acid. Thus, owing to its innate multiple-functional traits and its broad biocontrol activities, we found that this antagonistic strain isolated from the tobacco rhizosphere could establish itself successfully in the tomato rhizosphere to control soilborne diseases.

Multivariate analysis of original identification and chemical markers exploration of Chinese ginger

Mengqing Guo,Qian Shen,Yu Wu,Lili Li,Lin Zhang,Yu Wang,Yanjun Zhang,Pengwei Zhuang,Hong Guo 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.7

Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements. However, the content of active ingredients varied greatly from place to place. In this study, we first identified and compared the compositions of ginger samples from six different origins. Then, we evaluated the anti-inflammatory activity of different samples in LPS-stimulated RAW264.7 cells. The results indicated that highly variable in chemical composition and activity for ginger from different origin. Further, correlation analysis showed that isoborneol, terpineol, α-curcumene, germacrene D, α-elemol and 8-shogaol exhibited a strong correlation with inflammatory factors, which could be used as potential chemical markers to evaluate quality and distinguish source of ginger. Finally, comprehensive evaluation found that the ginger from Sichuan exerts stronger anti-inflammatory properties. This study will help to select ginger varieties with excellent characteristics, provide theoretical basis for the development and utilization of ginger.