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        Rapid Detection of Streptococcus mutans Using an Integrated Microfluidic System with Loop-Mediated Isothermal Amplification

        Wang Jingfu,Wang Jingyi,Chang Xin,Shang Jin,Wang Yuehui,Ma Qin,Shen Liangliang 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8

        Streptococcus mutans is the primary causative agent of caries, which is one of the most common human diseases. Thus, rapid and early detection of cariogenic bacteria is critical for its prevention. This study investigated the combination of loop-mediated isothermal amplification (LAMP) and microfluid technology to quantitatively detect S. mutans. A low-cost, rapid microfluidic chip using LAMP technology was developed to amplify and detect bacteria at 2.2–2.2 × 106 colony-forming units (CFU)/ml and its detection limits were compared to those of standard polymerase chain reaction. A visualization system was established to quantitatively determine the experimental results, and a functional relationship between the bacterial concentration and quantitative results was established. The detection limit of S. mutans using this microfluidic chip was 2.2 CFU/ml, which was lower than that of the standard approach. After quantification, the experimental results showed a good linear relationship with the concentration of S. mutans, thereby confirming the effectiveness and accuracy of the custom-made integrated LAMP microfluidic system for the detection of S. mutans. The microfluidic system described herein may represent a promising simple detection method for the specific and rapid testing of individuals at risk of caries.

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        Pull-off bond behavior of anchored random-chopped FRP composites bonded to concrete

        Wang, Zhen,Khalid, Hammad R.,Seo, J.H.,Kang, Jingfu,Lee, H.K. Elsevier 2018 COMPOSITE STRUCTURES -BARKING THEN OXFORD- Vol.185 No.-

        <P><B>Abstract</B></P> <P>An externally bonded random-chopped FRP composite can efficiently strengthen concrete structures, though premature debonding is commonly reported, which can be prevented using an anchorage system. In this paper, the pull-off bond strength of anchored random-chopped FRP composites bonded to concrete was tested by direct pull-off tests. The effects of different parameters, in this case the anchorage depth, the diameter of the FRP anchors, and the concrete compressive strength levels were investigated. Finally, analytical models from the literature were used to predict the pull-off bond behavior of the samples. The test results revealed that the FRP anchors efficiently prevented cohesive failures of random-chopped FRP composites-to-concrete bonded samples and significantly increased their pull-off bond strength compared to unanchored control samples. The prediction results of the pull-off bond behavior according to Kim and Smith’s model were in good agreement with the experimental data.</P>

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        Influence of water injection parameters on the performance of a water-lubricated single-screw air compressor

        Yanan Li,Jingfu Wang,Yuting Wu,Biao Lei,Ruiping Zhi,Lili Shen 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1

        A thermodynamic model of a water-lubricated single-screw air compressor was established to examine the impact of water injection parameters on performance. Heat transfer and leakage between humid air and water was considered by analyzing the impacts of rotation speed, discharge pressure, the rate of water injection, and diameter of droplets on the performance of a compressor. The discharge temperature could be reduced by increasing the rate of water injection, resulting in the compression procedure moving towards an isothermal state. The increase in the rate of water injection under rated conditions from 60 L/min to 80 L/min resulted in a reduction in the compressor discharge temperature, increased the volume efficiency, and increased adiabatic efficiency by 11.1 K, 1.5 %, and 2.8 %, respectively. Water injection atomization increased the area of transfer of heat between humid air and water and improved the performance of compressor.

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        Mathematical modeling of torque for single screw expanders

        Yuting Wu,Ruiping Zhi,Wei Wang,Lili Shen,Yeqiang Zhang,Biao Lei,Jingfu Wang,Chongfang Ma 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.1

        This paper presents a mathematical model of torque for Single screw expanders (SSEs). Instantaneous torque and torque ratio were analyzed and discussed. The periodic variation of instantaneous torque is the same for different inlet pressure levels. The torque ratio, with its value close to 1, is independent of the inlet pressure of SSE. An experimental system was established to measure the torque, power and shaft efficiency of the self-developed SSE prototype, and results were used to validate the model. Comparison shows that the difference between calculated and experimental torque values is small (6.58 N.m to 7.55 N.m). The calculated and experimental output power is similar, with a difference of 2.07 kW to 2.37 kW. Therefore, the proposed model can be used to estimate the torque and output power of SSEs.

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