鑛物性 冷却油의 Coolingability에 관한 硏究

김옥삼,최은순,정동화 麗水水産大學校産業技術硏究所 1992 産業基術硏究所 論文集 Vol.1 No.-

The laboratory quench method used in this study is based on plunging a silver cylinder heated to 800±1℃ into mineral base oils at given temperature. Experimental apparatus and measuring method followed the Korean Industrial Standard. Cooling curves at the boundary of the shape of cylinderical type measured by a silver-alumel thermocouple is recorded throughout the quenching period. Using the cooling curves, the collingability of the quenching oils at a various temperrature were compared and discussed.

허혈성 뇌졸중과 응고인자 XIII Val 34 Leu 다형성과의 무관성

방차옥,최종순,박형국,안무영,홍세용 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Background and purpose: Although a common G to T point mutation (FXIII Val34Leu) in exon 2 of the alpha-subunit of the FXIII is protective against myocardial infarction and venous thrombosis, there are few reported the association of FXIII Val34Leu and ischemic stroke. The aim of this study was to investigate the protective role of the FXIII Val34Leu against ischemic stroke and its suggested interaction with the plasminogen activator inhibitor-1 (PAI-1) 4G/5G polymorphism. Methods: We carried out genotype analyses for FXIII Val34Leu using single-stranded conformation polymorphism and 4G/5G polymorphism in the promoter region of the PAI-1 gene in 53 patients with ischemic stroke and in 82 race-matched control subjects. Results: Age, hypertension, diabetes, and low-density lipoprotein were significantly higher in ischemic stroke patients. However, genetic distributions of FXIII A subunit and promoter region of PAI-1 were not different on both groups. Even adjusting for age, sex, hypertension, diabetes, and total cholesterol, there were not significantly associated between FXIII Val34Leu or 4G/4G genotype of PAI-1 and ischemic stroke. Conclusion: FXIII Val34Leu and the 4G/4G genotype of the PAI-1 gene are not significantly associated with an increased or decreased risk of ischemic stroke.

김천산 자두의 성분과 그 특성

김순희,강병태,박동철,윤옥현,이재우,한만덕,최종동 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.1

Physicochemical properties and chemical compositions of two plums (Oishiwase, Formosa) produced in Kimcheon area were investigated. Chemical compositions of two plum cultivars were similar except nitrogen free extract(NFE). NFE value of Formosa was higher than Oishiwase. Alcohol insoluble substance content, total soluble pectin, total sugar content and total organic acid content of Oishiwase were 2.08%. 14,16%, 5.07% and 949.16mg%, respectively. In case of Formosa cultivar, those were 50%, 15.42%, 5.91% and 985.91mg%, respectively. Fructose content among free sugars of two plum cultivars range from 3.97~3.45% and the other were glucose, sucrose and inositol, 1.83~1.53%, 0.08~0.05% and 0,04~0.039%, respectively. Malic acid content was 35~373mg% and others were levulinic acid, lactic acid, citric acid, succinic acid and fumaric acid in the decreasing order.

교정부호가 있는 한영문서의 인식 알고리즘

박종원,박순옥,권영희 충남대학교 1991 忠南科學硏究誌 Vol.18 No.2

The purpose of this paper is to recognize Korean/English texts including Proof-correction marks and to correct the texts according to proof-correction marks : leaving space between the words, deleting space between the characters deleting characters mark. For the recognition of the marks, detects mark's boundary pixels first, then extract Features-slant direction and its changes-using depth histogram and its difference value. And the algorithm processes text's proof correction and its re-arrangement. As a result of the recognition, the algorithm recongnizes hand-generated Proof-correction marks exactly and printed Korean and English characters more than 97%.

괄루근렉틴의 정제 및 성질에 관한 연구

박종옥,이순옥 慶星大學校 1992 論文集 Vol.13 No.3

A lectin from root of Trichosanthes Kirilowii (Trichosanthes Radix:TR) was purified by using (NH₄)₂SO₄precipitation, ionexchange chromatography on DEAE Sephadex A-50, hydroxyapatite chromatography on and filtration on Sephacryl S-200 column chromatography. The purification acheived 47.34 folds from crude homogenate with a yield of 5.4% and appeared to be homogeneous on polyacrylamide gel electrophoresis. The lectin agglutinated human erythrocytes of all types (A 〈B, AB, 0). The molecular weight of TR lectin was estimated as about 60,000 dalton by gel filtration, and 29,500 dalton by SDS-polyacrylamide gel electrophoresis. The hemagglutinating activity of lectin was inhibited by sugars, lactose, galactose and its derivatives, EDTA and cations; ?? , ?? , ?? , ?? . The TR lectin was stable over a wide pH ranges and below 65℃.

Saccharomyces cerevisiad Quinone Reductase에 관한 연구

김경순,박종옥,석희원 慶星大學校 1998 論文集 Vol.19 No.1

Saccharomyces cerevisiae로부터 quinone reductase를 정제하여 물리화학적 성질을 조사하였다. 정제된 효소는 polyacrylamide gel electrophoresis를 시행한 결과 하나의 주된 band를 나타내었고, gel filtration에 의해 측정된 이 효소의 분자량은 111,000 dalton 이었다. 또한 SDS-PAGE에 의해 측정된 subunit의 분자량은 22,000 dalton 이었고, 최적 pH는 6.0이었으며, 1,4-benzoquinone과 1,4-naphthoquinone에 대한 Km값은 각각 1.3M과 14.3?M인 것으로 나타났다. Quinone and related compounds have received considerable attention recently, due to their widespread environmental occurrence and toxicological potential. Evidence has favored the hypothesis that quinone reductase is protective against quinone and quinone imine toxicity, by virtue of a two-electron reduction to the hydroquinone, thereby facilitating conjugation with glucuronide, partitioning into the aqueous phase, and excretion. In addition, it has been suggested that hepatic cytosolic quinone reductase has a functional role in ethanol metabolism and decreases hepatotoxicity. Furthermore, a water-soluble quinone, coenzyme Qo, was shown to stimulate insulin release, and it was suggested that quinone reductase might play some role in physiological insulin release. An intracellular, soluble quinone reductase was purified from S. cerevisiae and some properties of the enzyme were examined. The enzyme exhibited a native molecular mass of approximately 111kDa as determined by gel filtration on a Sephacryl 200 column. The subunit molecular mass was estimated by SDS-PAGE to be 22kDa. The effect of pH on activity was measured with phosphate buffer. The optimum pH was shown to be pH 6.0. The apparent Km for 1,4-benzoquinone and 1,4-naphthoquinone are 1.3 mM and 14.3?M, respectively.

국민학교 「자연」 교과서 개발체제 분석 및 평가 연구

우종옥,정완호,권재술,최병순,정진우,허명 한국교원대학교 과학교육연구소 1992 청람과학교육연구논총 Vol.2 No.1

Elementary science textbooks are the major instructional materials which contain sceientific objectives to be acquired by students. The purpose of this study is to analyze the development system, to evaluate the inquiry activity in current elementary science testbooks, and to make suggestions for the improvement of them. The questionnaires were administered to 116 subjects that participated in development of the 5th elementary science textbooks, and 86 of the subjects responded. Based on the data gathered and analyzed in this study, the major findings are as follows. First, regarding the analysis and evaluation of elementary science textbooks. 1. The instructional objectives in elementary science textbooks are analyzed. The proportions of comprehension objectives is 38%, scientific inquiry process objectives 21%, experimental skills objectives 7%, scientific attitudes and interests objectives 4%. 2. The science concepts in elementary science textbooks of the 3rd grade required the preconcrete operational level(82%) and of the 6rd grade required the postconcrete operational level (73%) by J. Piaget. 3. The inquiry activities in elementary science textbooks are emphasizing gathering and organizing results and evaluation, and hypothesizing and designing an experiment. Inquiry index of the elementary science textbooks is 76. This is significantly higher than 35∼50 of America and U.K. 4. The number of questions per class hour is mostly three, and most of them require the students to explain phenomena in nature or in experiment Second, regarding the development system of elementary science textbooks. 1. The budget for and the period of the development of elementary science textbook should be expanded. 2. For the improvement of elementary science textbooks, more abundant resources and time should be used for the basic study and the field trial of textbooks. 3. The elementary science textbook must include the affective objectives as well as the scientific knowledges and scientific inquiry skills. 4. It is not desirable to assign equal number of pages to the content of physics, chemistry, biology, and earth science. 5. Closer cooperation system is needed among the ministry of education, development research center and the field trial schools.

김치가 노화촉진쥐 간의 유리기 생성 및 항산화효소 활성에 미치는 영향

김종현,권명자,이소영,류재두,문갑순,최홍식,송영옥 부산대학교 김치연구소 2002 김치의 과학과 기술 Vol.8 No.-

본 연구는 김치가 노화 억제에 미치는 영향을 살펴보기 위하여 AIN-76마우스 식이에 배추, 갓김치, 갓배추김치를 각 동결건조 김치를 5%씩 첨가하여 만든 후 김치식이를 노화촉진쥐인 SAMP8에게 1년간 섭취시키면서 간의 노화관련 특성인 유리기 농도와 항산화효소계의 효소활성 변화를 살펴 보았다. 노화촉진쥐 간의 총유리기, OH radical, H_2O_2 그리고 TBARS모두 가령에 따라 증가되었으며, 간에서 생성된 유리기 농도와 TBARS 농도는 대조군에 비해 김치섭취시 유의적으로 감소되었다(p<0.05). 그러나 생성된 유리기를 제거하기 위한 SOD활성은 오히려 김치섭취군에서 유의적으로 증가되었으며(p<0.05), catalase와 GSH-px, GSH/GSSG의 비율도 김치섭취군에서 유의적으로 증가하였다(p<0.05). 따라서 김치섭취는 노화에 따라 발생되는 간의 유리기 및 TBARS 생성은 억제시키면서, 이를 제거하는 항산화계 효소는 활성화시켜 노화를 억제한 것으로 생각된다. 김치종류 중에서는 배추김치보다 배추갓김치와 갓김치가 유리기 생성 억제 및 항산화 효소 활성을 더욱 증진시킨 것으로 관찰되어 이는 첨가된 갓의 항산화물질 때문인 것으로 생각된다. This study was performed to investigate the effect of kimchi intake on antiaging characteristics in the brain of senescence-accelerated mouse (SAM) in terms of free radical production and anti-oxidative enzymes. Two hundreds twenty SAM (20 mice) were divided into four groups and fed kimchi diet for 12 months. Experimental groups were kimchi free AIN-76 diet (control) group, Korean cabbage kimchi diet (KCK)group, 30% mustard leaf added Korean cabbage kimchi diet (MKCK) group, and mustard leaf kimchi diet (MLK) group. Concentrations of total free radical, OH radical and H_2O_2 of control group increased up to 123%, 262% and 174% of initial value (p<0.05) after one year. Increase in free radical production in kimchi groups due to aging was decreased by kimchi feeding. Among kimchi groups. MKCK and MLK groups showed greater inhibiting effect against free radical production than KCK. The concentration of TBARS in the bruin of control group also significantly increased up to 362% of initial value as aged (p<0.05) and production of TBARS in kimchi groups were decreased. When the activities of Cu, Zn-SOD, Mn-SOD, GSH-px and catalase of kimchi groups were compared to those of control at the same experimental period, anti-oxidative enzyme activities of kimchi groups were lower than those of control (p<0.05). But GSH/GSSG in kimchi groups were higher compared to control. In conclusion, decrease in free radical production and increase in anti-oxidative enzyme activities were observed from kimchi groups suggesting that kimchi might have important role on retarding aging. Among kimchi variety tested in this experiment, MKCK and MLK seem to have greater effect on inhibiting free radical production and increasing anti-oxidative enzyme activities than KCK. Further study is needed to find out the mechanism of kimchi on retarding aging.

초·중·고 학생들의 과학 탐구 능력 추이 분석을 위한 종단적 연구

우종옥,김범기,허명,김찬종,양일호,최관순,김태선 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

본 연구에서는 우리 나라 초·중·고등학교 학생들의 과학 탐구 능력에 대한 종단적 추이를 분석하였다. 1997년에는 우종옥 등(1998)이 개발한 검사지를 사용하였으며, 1999년에는 이를 일부만 수정하여 사용하였다. 조사 대상은 성별, 지역별 등을 고려하여 표집하였다. 조사된 자료는 각각 횡단적으로 분석하고, 이를 바탕으로 학생들의 과학 탐구능력이 어떻게 변해 가는지 종단적 추이를 분석하였다. 조사 결과 초등학생들의 과학 탐구 능력은 유의미한차이가 없었으나, 중·고등학생들의 과학 탐구 능력은 유의미하게 향상된 것으로 나타났다. 학교 과학 교육의 질을 과학적으로 관리하기 위해서는 교육과정이 추구하는 교육 목표의 달성이 성공적으로 이루어지고 있는지를 국가수준에서 주기적으로 측정하여 부진의 원인을 진단하고, 개선 방안을 계속 연구하여야 할 것이다. The longitudinal trend of elementary and secondary students' science process skills were investigated to monitor the progress of science learning. Science process skill test, developedin an earlier study on developing a national science process skills assessment system, was revised and administered for the study on March, 1999. Subjects were sampled nation wide according to sex and region. The results of this assessments were compared to those administered on February, 1997. The results showed little difference on science process skills among elementary school students. However, secondary students showed marked increase in science process skills. More systematic and long-term research for assessing science process skills should be planned and performed periodically to provide empirical and foundational data for educational policy making.

척골 지단 신경의 전기생리학적 연구

김종순,이현옥,안소윤,구봉오,남건우,김호봉,류재관,류재문 대한정형도수치료학회 2005 대한정형도수물리치료학회지 Vol.11 No.2

The ulnar nerve extends down the arm, across the elbow, and into the hand. It provides sensation to the little and ring fingers and activates many of the small muscles in the hand. The determination of peripheral nerve conduction velocity is an important part of ulnar nerve evaluation. The electrodiagnostic value as neurophysiologic investigative procedure has been known for many years but normal value of digital nerve was not reported in korea. The purpose of this investigation was to measure the digital nerve conduction velocity of ulnar nerve for obtain clinically useful reference value and compare difference in each fingers and then compare with the other countries. 71 normal Korean volunteers (age, 19-65 years; 142 hands) examined who has no history of peripheral neuropathy, diabetic mellitus, chronic renal failure, endocrinedisorders, anti-cancer medicine, anti-tubercle medicine, alcoholism, trauma, radiculopathy. Nicolet Viking Ⅱ(EMG machine) was use for detected conduction velocity and amplitude of digital nerve in ulnar nerve. Data analysis was performed using SPSS. Descriptive analysis was used for obtain mean and standard deviation and independent t-test was used to compare with ring and little finger. Conduction velocity of the right ring finger was 57.44m/sec and little finger was 55.32msec. The left ring finger was 55.55msec and little finger was 54.11msec. Amplitude of the right ring finger was 30.28µV and little finger was 48.36µV. The left ring finger was 30.67µV and little finger was 57.76µV. There were significantly difference between ring and little in amplitude (p<.05) but there were no statistically difference between conduction velocity of ring and little finger (p>.05). The amplitude of little finger are greater than ring finger. The present results revealed that electodiagnosis can easily perform in little finger for digital nerve of ulnar nerve study.