인공 치아우식 발생 모델에서 디지털 방사선 공제술을 이용한 인접면 치아우식증의 진단

박정훈,최용석,황의환,이기자,최삼진,박영호,김경숙,진현석,홍경원,오범석,박헌국 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.1

Purpose : The purpose of the experiment was to evaluating the diagnostic ability of dental caries detection using digital subtraction in the artificial caries activity model. Materials and Methods : Digital radiographies of five teeth with 8 proximal surfaces were obtained by CCD sensor (Kodak RVG 6100 using a size #2). The digital radiographic images and subtraction images from artificial proximal caries were examined and interpreted. In this study, we proposed novel caries detection method which could diagnose the dental proximal caries from single digital radiographic image. Results : In artificial caries activity model, the range of lesional depth was 572-1,374 μm and the range of lesional area was 36.95-138.52mm². The lesional depth and the area were significantly increased with demineralization time (p<0.001). Furthermore, the proximal caries detection using digital subtraction radiography showed high detection rate compared to the proximal caries examination using simple digital radiograph. Conclusion : The results demonstrated that the digital subtraction radiography from single radiographic image of artificial caries was highly efficient in the detection of dental caries compared to the data from simple digital radiograph.

급성 골수성 백혈병에서 Adenosine deaminase 측정의 임상적 유용성

고광상,박정오,강성훈,김재석,박순철,서연실,김기헌,조무자 임상병리검사과학회 1999 임상병리검사과학회지 Vol.31 No.2

배경 : Adenosine deaminase(ADA) 는 adeno-sine을 inosine으로 deamination하는 반응을 촉매하는 효소로, 혈액 종양을 비롯한 결핵, 간 질환, 등의 다양한 질환에서 활성도가 증가하는 것으로 알려져 있으며, 여러 질환에서 연구가 이루어지고 있으나 측정의 기술적 어려움으로 이용이 확대되지 못하였다. 그러나 최근 고안된 자동 화학 분석기를 이용한 ADA 와 동위효소의 분별정량 방법으로 ADA 측정의 임상적 적용이 용이 해졌다. 저자들은 이 방법으로 혈장을 이용하여 AML에서의 ADA활성도와 동위효소의 분포양상의 특성과, 치료경과에 따른 변화를 관찰하여 AML에서 ADA측정의 임상적 유용성을 알아보고자 하였다. 방법 : AML 환자군 16 예와 정상 대조군 25 예의 혈장 ADA와 동위효소의 활성도를 측정 비교하고, 환자군중 10 예에서는 치료 후 1주 간격으로 최고 8 주까지 추적 관찰을 시행하였다. ADA측정은 자동화학 분석기로 발색 정량하고, 동위효소의 측정은 ADA_(1) 선택적 억제제인 EHNA를 첨가하여 ADA_(2)를 측정하는 방법을 이용하였다. 결과 : AML 환자 군 16명의 혈장 ADA 활성도는 34.0±20.8IU/L로 정상 대조군 25명의 혈장 ADA 활성도 14.28±3.26IU/L에 비해 유의하게 높았다. 정상 대조군에서는 ADA2 가 75.0%로 우세한 것에 비해 AML군에서는 ADA_(1)이 62.5%로 우세하였고, ADA_(1)은 모두 정상대조군보다 높은 값을 보였다 . AML 환자 군 중 추적 검사를 시행하였던 10 예 (완전관해 5예 , 불완전관해 5예)에서 ADA 값과 ADA1이 10예 모두에서 4주 안에(평균 2.8주) 정상범위로 감소하였고, 5 예에서 ADA활성도가 정상범위로 감소한 후 1 주 후까지 ADA_(1)활성도가 정상범위 이상으로 지속되었다. 결론 : AML의 진단과 경과 추적에 혈장에서 측정한 ADA활성도와 동위효소 분포 양상을 이용할 수 있으며, 특히 ADA_(1)이 total ADA나 ADA_(2)보다 더 유용함을 알 수 있었다. ADA 활성도는 치료 후 관해여부와 관계없이 세포수가 감소함에 따라 모두 감소하므로 관해 여부를 예측 할 수는 없으나, 재발을 검출할 목적으로 이용할 수 있으며, 이때는 ADA 활성도가 모두 정상범위로 감소하는 치료 후 4 주 이후부터 검사하는 것이 효율적이라고 생각된다. Adenosine deaminase(ADA) activiteis in sera are increased in various disease such as hematologic malignancies, tuberculosis, and liver diseases. To evaluate the clinical value of measuring ADA and its isoenzyme activites in plasma with the automated assay in acute myeloid leukemia, we compared ADA and its isoenzyme activities in normal control group with those in acute myeloid leukemia group, and followed up those during chemotherapy in AML group. The measurements of plasma ADA and its enzymes, especially ADA_(1) were useful for AML diagnosis. But they were not valuable markers to discriminate between complete remission and incomplete remission. For the detection of relapse, it could be valuable to measure ADA activities beyond 4 weeks after chemotherapy.

제주 전통된장의 품질 특성

오현정(Hyun Jeong Oh),임자훈(Ja Hun Lim),이주연(Ju Yeon Lee),전시범(Si Bum Jeon),강혜영(Hye Young Kang),오유성(You Sung Oh),오영주(Young Ju Oh),임상빈(Sang Bin Lim) 한국조리학회 2009 한국조리학회지 Vol.15 No.2

Midazolam 과 Ketamine 을 이용한 화학적 안면 박피술시 Esmolol 이 심혈관계와 중추신경계에 미치는 효과

오인영,신혜원,조헌,장성호,임혜자,김난숙,우영철 대한마취과학회 1995 Korean Journal of Anesthesiology Vol.29 No.1

One of the indications of outpatient surgery is chemical peeling of the face. It usually requires deep sedation with analgesia of short duration. For this purpose we tried to use ketamine(1 mg/kg) and midazolam(0.1 mg/kg) but experienced frequent hypertension and tachycardia. We tried to use beta blocker, esmolol for the of control blood pressure and heart rate. The patients were divided into two groups. In control group, eighty-five patients were anesthetized with bolus intravenous injection of I mg/kg of ketamine and 0.1 mg/kg of midazolam. In esmolol group, eighty-three patients reed a continuous infusion of esmolol(500 mcg/kg/min for initial dose, followed by 200 mcg/kg/min for maintenance) in addition to midazolam(0.1 mg/kg) and ketamine(1 mg/kg). During operation, we observed systolic, diastolic blood pressure and peripheral oxygen saturation(SaO_2). All patients were allowed to breathe spontaneously. There was no case of airway obstruction and SaO_2 value was over 98% in most cases. So we investigated the effects of esmolol on blood pressure,heart rate,emergence sequelae and patients acceptance in the anesthesia with ketamine and midazolam in the patient of facial chemical peeling. The results were as follows: 1) No one could recall. 2) There were no differences on the status of mood, emotion and patient's positive acceptance between two groups 3) Blood pressure and heart rate were increased during anesthesia with midazolam and ketamine. But esmolol attenuated this response, significantly(P$lt;0.05). We concluded that esmolol added benefits of control of blood pressure and heart rate in the anesthesia of facial chemical peeling. But the control of the high blood pressure is not so easy, so it is needed to find out the optimal dosage of esmolol for complete blocking of the sympathetic response without complications. (Korean J Anesthesiol 1995; 29: 64-69)

응급 내과환자의 저나트륨혈증

정자헌(Ja Hun Jung),손동현(Dong Hyun Sohn),박준용(Joon Yong Park),오광택(Kwang Taek Oh),한동수(Dong Su Han),양석철(Suck Chul Yang),손주현(Ju Hyun Sohn),김순길(Soon Kil Kim),김호중(Ho Jung Kim) 대한내과학회 1996 대한내과학회지 Vol.51 No.4

N/A Objectives: Hyponatremia is the most common finding of electrolyte disorder in patients admitted to medical ward. It is suggested that the prognosis of patients with hyponatremia is worse than that with normal sodium concentration in morbidity and mortality. We performed the study to evaluate the incidence and cause of hyponatremia in inpatients admitted to medical ward via emergency room with prospective study. Methods: Hyponatremia was defined as a serum sodium concentration equal to or less than 134 mEq/L. A total 225 medical patients were prospectively studied for 45 days. Other chemistry and electrolytes of blood and urine were determined within 24 hrs following admission. Results: 1) Of medical inpatients through emergency room, hyponatremia was detected in high frequency (16%) and mild hyponatremia (125-134 mEq/L) was showed in 90% or more. 2) The most common cause of hyponatremia was decreased effective circulating blood volume group (76%), and its common underlying disease was liver disease. 3) Compared to other groups, the group with decreased effective circulatory volume showed significant decrease in spot urine sodium concentration, fractional excretion of uric acid and serum creatinine concentration. 4) There was no correlation between serum concentration of hyponatremia and other parameters associated with it. As it was corrected, no significant simultaneous changes on BUN and serum uric acid concentration was noted. Conclusion: Interpretation of this data depend on not decreased circulatory volume but also other compounding factor as production and excretion of which is related to underlying variable disease.

An ANKRD11 exonic deletion accompanied by a congenital megacolon in an infant with KBG syndrome

Go Hun Seo,Arum Oh,Minji Kang,Eun Na Kim,Ja-Hyun Jang,Dae Yeon Kim,Kyung Mo Kim,Han-Wook Yoo,Beom Hee Lee 대한의학유전학회 2019 대한의학유전학회지 Vol.16 No.1

KBG syndrome is an autosomal dominant syndrome presenting with macrodontia, distinctive facial features, skeletal anoma-lies, and neurological problems caused by mutations in the ankyrin repeat domain 11 (ANKRD11) gene. The diagnosis of KBG is difἀcult in very young infants as the characteristic macrodontia and typical facial features are not obvious. The youngest patient diagnosed to date was almost one year of age. We here describe a 2-month-old Korean boy with distinctive craniofa-cial features but without any evidence of macrodontia due to his very early age. He also had a congenital megacolon without ganglion cells in the rectum. A de novo deletion of exons 5-9 of the ANKRD11 gene was identiἀed in this patient by exome sequencing and real-time genomic polymerase chain reaction. As ANKRD11 is involved in the development of myenteric plexus, a bowel movement disorder including a congenital megacolon is not surprising in a patient with KBG syndrome and has possibly been overlooked in past cases.

The Effect of Extracellular Glutamate Release on Repetitive Transient Ischemic Injury in Global Ischemia Model

Gi Ja Lee,Seok Keun Choi,Yun Hye Eo,Sung Wook Kang,Samjin Choi,Jeong Hoon Park,Ji Eun Lim,Kyung Won Hong,Hyun Seok Jin,Berm Seok Oh,Hun Kuk Park 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.1

During operations, neurosurgeons usually perform multiple temporary occlusions of parental artery, possibly resulting in the neuronal damage. It is generally thought that neuronal damage by cerebral ischemia is associated with extracellular concentrations of the excitatory amino acids. In this study, we measured the dynamics of extracellular glutamate release in 11 vessel occlusion (VO) model to compare between single occlusion and repeated transient occlusions within short interval. Changes in cerebral blood flow were monitored by laser-Doppler flowmetry simultaneously with cortical glutamate level measured by amperometric biosensor. From real time monitoring of glutamate release in 11 VO model, the change of extracellular glutamate level in repeated transient occlusion group was smaller than that of single occlusion group, and the onset time of glutamate release in the second ischemic episode of repeated occlusion group was delayed compared to the first ischemic episode which was similar to that of single 10 min ischemic episode. These results suggested that repeated transient occlusion induces less glutamate release from neuronal cell than single occlusion, and the delayed onset time of glutamate release is attributed to endogeneous protective mechanism of ischemic tolerance.

Nematicidal and Plant Growth-Promoting Activity of Enterobacter asburiae HK169: Genome Analysis Provides Insight into Its Biological Activities

( Mira Oh ),( Jae Woo Han ),( Chanhui Lee ),( Gyung Ja Choi ),( Hun Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6

In the course of screening for microbes with nematicidal activity, we found that Enterobacter asburiae HK169 displayed promising nematicidal activity against the root-knot nematode Meloidogyne incognita, along with plant growth-promoting properties. Soil drenching of a culture of HK169 reduced gall formation by 66% while also increasing root and shoot weights by 251% and 160%, respectively, compared with an untreated control. The cell-free culture filtrate of the HK169 culture killed all juveniles of M. incognita within 48 h. In addition, the nematicidal activity of the culture filtrate was dramatically reduced by a protease inhibitor, suggesting that proteolytic enzymes contribute to the nematicidal activity of HK169. In order to obtain genomic information about the HK169 isolate related to its nematicidal and plant growth-promoting activities, we sequenced and analyzed the whole genome of the HK169 isolate, and the resulting information provided evidence that the HK169 isolate has nematicidal and plant growth-promoting activities. Taken together, these observations enable the future application of E. asburiae HK169 as a biocontrol agent for nematode control and promote our understanding of the beneficial interactions between E. asburiae HK169 and plants.

AFM Imaging Analysis of Alopecia Areata by Edge Detection

( Gi Ja Lee ),( Hwi Jun Kim ),( Yun Hye Eo ),( Sam Jin Choi ),( Ki Heon Jeong ),( Bark Lynn Lew ),( Woo Young Sim ),( Mu Hyoung Lee ),( Berm Seok Oh ),( Hun Kuk Park ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1

Alopecia areata(AA) is a common type of autoimmune disorder that produced sudden patch-like hair loss. Owing to the dysfunction of keratogenous zone in affected hair at early anagen, hair shaft with AA had abnormal structure or total disintegration. Typically, it showed characteristic(or unique) broken hair shafts on the hair-less patch. Few techniques were introduced to investigate hair surface. Recently, atomic force microscopy(AFM) became an ideal method for non-invasive examination of hair surface. When the cortical keratinocytes were affected in AA, the topographic changes of hair cuticles could be examined by AFM in nanoscale. In this experiment, we compared the AFM images of hair surface and extracted parameters from the cuticle between control group and patient group with AA(n=12, each). Data demonstrated that the shaft surface of AA patient`s hair was more damaged than that of normal group. Various types of damage such as crack of scale, longitudinal striation, endocuticular ghost and debris were observed on hair cuticles with AA. In order to find cuticle parameters, we performed the edge detection of cuticle with canny mask. The curvature was defined as a secondary differentiation with the x and y coordinates of cuticle edge on AFM images to compare the cuticle edge between two groups. As a result, the cuticle scale parameters showed frequent changes in AA patient groups. In particular, top distance and step height of cuticle in AA group were lower than those of control group and the curvature of cuticle edge in AA patient group was higher than those of healthy one. In conclusion, the cortical keratinocytes might affect the pathogenesis of AA. This is the first comparison study about hair shaft surfaces over the whole lengths between AA and healthy group, to our knowledge.

Evidence of Toxoplasma gondii Exposure Among Horses in Korea

Seung Hun Lee,Sang Eun Lee,Min Goo Seo,Gil Jae Cho,Oh Deog Kwon,Dong mi Kwak,Won Ja Lee 대한수의학회 2014 대한수의학회 학술대회발표집 Vol.2014 No.-