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고화방지제의 첨가가 참다래 동결건조분말의 물리화학적 특성에 미치는 영향
오현정(Hyun Jeong Oh),임자훈(Ja Hun Lim),이주연(Ju Yeon Lee),오영주(Young Ju Oh),임상빈(Sang Bin Lim) 한국조리학회 2014 한국조리학회지 Vol.20 No.1
Two kinds of anticaking agents (dextrin, polydextrose) were combined with kiwifruit paste at 5% w/w ratio and freeze-dried to prepare a powdered material. The physiochemical characteristics of kiwifruit powders with anticaking agents were compared with those without anticaking agents as the control. The yield was higher in the powders with anticaking agents than the control. Moisture content, acidity, and total phenolics were lower in the powders with anticaking agents than the control. The contents of vitamin C was higher in the powders with anticaking agents than the control, but was no significant difference with different anticaking agent types. There were no significant differences in free sugar content (fructose, glucose, total sugar) and organic acid content (oxalic acid, lactic acid, total) depending on the anticaking agent types. Hunter`s L-value was significantly high in the order of the samples with dextrin, the control, and polydextrose, while a-values howed an opposite tendency. Browning index, water solubility, and swelling power didn`t show any significant difference. However, the hygroscopicities with elapsed time were lower in the powders with anticaking agents than the control. Therefore, the kiwifruit powder combined with dextrin or polydextrose as an anticaking agent at 5% w/w ratio could be used as a food biomaterial with a good quality in moisture, vitamim C, color value, browning index, water solubility, and hygroscopicity.
황준호(Joon-Ho Hwang),오유성(You-Sung Oh),임자훈(Ja-Hun Lim),박지은(Ji-Eun Park),김미보(Mi-Bo Kim),윤훈석(Hoon Seok Yoon),임상빈(Sang-Bin Lim) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.12
제주재래식된장의 생리적 특성을 측정하기 위하여 제주도내 전통된장업체로부터 된장제품 4종(JTD1-4)을 수거하여, 유기용매로 추출, 분획하여 총 페놀 함량, 항산화활성(DPPH 유리기 소거능, superoxide 유리기 소거능), ACE 및 α-glucosidase 저해능, 항염 및 항암효과를 측정하였다. 에틸아세테이트 분획물에서 총 페놀 함량(201.8~305.4 ㎍/ g)과 DPPH 유리기 소거활성(37.5~59.9%)이 가장 높았다. Superoxide anion radical 소거활성은 부탄올 분획물에서 56.3~85.1%로 가장 높았는데, JTD1(81.7%)과 JTD4(85.1%)의 소거활성은 양성대조구로 사용한 ascorbic acid의 소거활성(82.2%)과 유사하였다. ACE 저해활성은 메탄올 추출물에서 23.6~74.5%로 가장 높았다. α-Glucosidase 저해활성은 디클로로메탄 분획물에서 72.0~80.2%로 양성대조구인 acarbos의 57.4%보다 높았다. RAW 264.7 세포를 대상으로한 NO 생성 저해활성(IC50: 17.3~29.0 ㎍/mL)은 에틸아세테이트 분획물이 가장 높았지만, 세포독성은 모든 분획물에서 관찰되지 않았다. 에틸아세테이트 분획물의 세포증식 저해활성은 정상세포주인 피부섬유아세포(HS-68)에서 JTD1-3은 100 ㎍/mL 이하의 농도에서 나타내지 않았으나, JTD4는 21%의 세포독성을 나타내었다. 대장암 세포주(HT-29)에 대해서는 JTD1과 JTD2는 세포증식이 저해되지 않았으나, JTD3과 JTD4는 50 ㎍/mL 이상에서 증식저해 효과를 나타내었다. 유방암 세포주(MCF-7)는 100 ㎍/mL에서 JTD3은 30.9%, JTD4는 34.7%의 세포증식 저해효과를 보였다. Four Jeju traditional Doenjangs (JTD), a traditional fermented soybean paste, were extracted and fractionated using organic solvents, and their physiological properties were evaluated such as total phenolic contents (TPC), antioxidant capacity (DPPH and superoxide free radical scavenging), inhibitory effect of ACE and α-glucosidase, and anti-inflammatory and anti-cancer activities. Ethyl acetate fractions had the highest TPC (201.8~305.4 ㎍/g) and DPPH radical scavenging activity (37.5~59.9%). Buthanol fractions had the highest superoxide anion radical scavenging activity (56.3~85.1%), and the activities of JTD1 (81.7%) and JTD4 (85.1%) were almost the same as that of ascorbic acid (82.2%) as the reference material. Inhibitory activities of ACE were 23.6~74.5% in methanol extracts, and those of α-glucosidase were 72.0~80.2% in dichloromethane fractions compared with 57.4% in acarbos as the reference. Inhibitory activities of NO production in LPS-induced RAW 264.7 were the highest as 17.3~29.0 ㎍/mL (IC50) in ethyl acetate fractions, and cell toxicities were not detected in all fractions. Ethyl acetate fractions of JTD1-3 showed no inhibitory activities, but that of JTD4 showed 21% of inhibitory activity of cell growth against human genital skin fibroblast cell line HS-68. Cell growth of human colon cancer cell line HT-29 was not inhibited by ethyl acetate fractions of JTD1 and 2, but was inhibited by those of JTD3 and 4 at more than 50 ㎍/mL. JTD3 and JTD4 showed the growth inhibition of breast adenocarcinoma cell line (MCF-7) by 30.9% and 34.7% at 100 ㎍/mL, respectively.