급성 골수성 백혈병에서 Adenosine deaminase 측정의 임상적 유용성

고광상,박정오,강성훈,김재석,박순철,서연실,김기헌,조무자 임상병리검사과학회 1999 임상병리검사과학회지 Vol.31 No.2

배경 : Adenosine deaminase(ADA) 는 adeno-sine을 inosine으로 deamination하는 반응을 촉매하는 효소로, 혈액 종양을 비롯한 결핵, 간 질환, 등의 다양한 질환에서 활성도가 증가하는 것으로 알려져 있으며, 여러 질환에서 연구가 이루어지고 있으나 측정의 기술적 어려움으로 이용이 확대되지 못하였다. 그러나 최근 고안된 자동 화학 분석기를 이용한 ADA 와 동위효소의 분별정량 방법으로 ADA 측정의 임상적 적용이 용이 해졌다. 저자들은 이 방법으로 혈장을 이용하여 AML에서의 ADA활성도와 동위효소의 분포양상의 특성과, 치료경과에 따른 변화를 관찰하여 AML에서 ADA측정의 임상적 유용성을 알아보고자 하였다. 방법 : AML 환자군 16 예와 정상 대조군 25 예의 혈장 ADA와 동위효소의 활성도를 측정 비교하고, 환자군중 10 예에서는 치료 후 1주 간격으로 최고 8 주까지 추적 관찰을 시행하였다. ADA측정은 자동화학 분석기로 발색 정량하고, 동위효소의 측정은 ADA_(1) 선택적 억제제인 EHNA를 첨가하여 ADA_(2)를 측정하는 방법을 이용하였다. 결과 : AML 환자 군 16명의 혈장 ADA 활성도는 34.0±20.8IU/L로 정상 대조군 25명의 혈장 ADA 활성도 14.28±3.26IU/L에 비해 유의하게 높았다. 정상 대조군에서는 ADA2 가 75.0%로 우세한 것에 비해 AML군에서는 ADA_(1)이 62.5%로 우세하였고, ADA_(1)은 모두 정상대조군보다 높은 값을 보였다 . AML 환자 군 중 추적 검사를 시행하였던 10 예 (완전관해 5예 , 불완전관해 5예)에서 ADA 값과 ADA1이 10예 모두에서 4주 안에(평균 2.8주) 정상범위로 감소하였고, 5 예에서 ADA활성도가 정상범위로 감소한 후 1 주 후까지 ADA_(1)활성도가 정상범위 이상으로 지속되었다. 결론 : AML의 진단과 경과 추적에 혈장에서 측정한 ADA활성도와 동위효소 분포 양상을 이용할 수 있으며, 특히 ADA_(1)이 total ADA나 ADA_(2)보다 더 유용함을 알 수 있었다. ADA 활성도는 치료 후 관해여부와 관계없이 세포수가 감소함에 따라 모두 감소하므로 관해 여부를 예측 할 수는 없으나, 재발을 검출할 목적으로 이용할 수 있으며, 이때는 ADA 활성도가 모두 정상범위로 감소하는 치료 후 4 주 이후부터 검사하는 것이 효율적이라고 생각된다. Adenosine deaminase(ADA) activiteis in sera are increased in various disease such as hematologic malignancies, tuberculosis, and liver diseases. To evaluate the clinical value of measuring ADA and its isoenzyme activites in plasma with the automated assay in acute myeloid leukemia, we compared ADA and its isoenzyme activities in normal control group with those in acute myeloid leukemia group, and followed up those during chemotherapy in AML group. The measurements of plasma ADA and its enzymes, especially ADA_(1) were useful for AML diagnosis. But they were not valuable markers to discriminate between complete remission and incomplete remission. For the detection of relapse, it could be valuable to measure ADA activities beyond 4 weeks after chemotherapy.

두 Streptomyces Strains에 의한 다핵 방향족 탄화수소화합물의 분해

김태전,김승곤,배형준 임상병리검사과학회 2002 임상병리검사과학회지 Vol.34 No.2

The purpose of this study was to investigate the degradation abilities of polynuclear aromatic hydrocarbon (PAHs; anthracene, phenanthrene, benzo(a)pyrene) compounds using Streptomyces halstedii scabies SAI-36, Streptomyces avendulas SA2-14, and Streptomyces badius (ATCC 39117, control group). Benzo(a)pyrene and anthracene showed a the degradation ability of 99.5% and 99.2% respectively by Streptomyces lavendulas SA2-14. Benzo(a)pyrene showed the degradation ability of 98.0% by Streptomyces halstedii scabies SAI-36. Anthracene showed the lowest degradation ability of 47.7% by Streptomyces badius(ATCC 39117). Benzo(a)pyrene and phenanthren showed the highest degradation abilities of 71.7% and 78.8% respectively. The results suggested that two experimental strains demonstrated superior abilities above the control group in degradation of PAHs compounds and Streptomyces lavendulas SA2-14 was the superior strain between the two experimental strains. The results also proposed the possibility of the use of actinomycetes in the degradation of non-degradable materials. These results were consistent with previous research results that Streptomyces lavendulas SA2-14 was the best strain in degradation ability of lignin, decoloration abilities of different dyes, different enzyme production abilities and degradation abilities of phenol compounds. It is suggested that more basic research is needed for actinomycetes to be used as a biological degradation.

5일 보존용 채혈백에 따른 보존일자별 농축혈소판의 생화학 및 성상 분석

이동범,유태종,김태전,김승곤,신종관,양대형,고광임,박성훈 임상병리검사과학회 2002 임상병리검사과학회지 Vol.34 No.2

Biochemical changes and icon of platelets on platelet concentrates preserved for a 5day term in several plastic containers were investigated. The authors evaluated the TOTM-PVC second generation container produced by Green Cross Medical Corp. and Boin medica. Blood obtained from 40 persons was manufactured for platelet concentrates within 4 hours and each divided into 20 units of platelet concentrations. Samples were taken at 0, 3, and 5days and the platelet count, pH, pO_(2), pCO_(2), HCO_(3)-, glucose, LDH, lactate, electrolytes, and mineral element were tested The number and MPV, for platelets icon, were well preserved on two different platelet concentrates during storage. A test for biochemical function appeared to be effective data. pH was maintained above 6.8 on two different platelet concentrates during storage. The results suggest that the TOTM-PVC second generation platelets storage container produced by Green Cross Medical Corp. and Boin medica was able to preserve platelets for at least five days without lesion and was it concluded to have biochemical efficacy on patients with transfusions.

Allergen 유발 단백질의 분리에 관한 검토

박정오 임상병리검사과학회 2000 임상병리검사과학회지 Vol.32 No.3

The food allergy as a clinical entity has been recognized for many years, although there is yet no general consensus as to the incidence of this syndrome. To evaluate the causative food allergens were studied by using dietary protein-dependent allergy. As the most common food allergen, followed by the allergens of korea's yellow soy beans, powdered red peppers, chestnuts, pine needles, young pumpkins and black peppers were the listed on the 3 -75KDa, According to the protein electrophoresis method. The author attempted to develope a food allergen that is indicative of evaluating IgE-specifically dependent on the causative food allergens.

갑상선질환의 세침흡인도말검사에서 질환별 특징적인 소견에 관한연구 : 유두상 암종과 다른 질환과의 비교를 중심으로

백운철,김성철,김태근,유숙,노정환,김태전 임상병리검사과학회 2002 임상병리검사과학회지 Vol.34 No.2

This study observed appearance existence and non-existence of nuclear groove, intranuclear pseudoinclusion, papillae, psammoma body, lymphocyte, multinucleated giant histiocyte and follicular structure that is a feature of cytologic change in FNAC of several thyroid nodules, and analyzed cytologic features of other thyroid diseases in comparison with papillary carcinoma of the thyroid The FNAC total in 103cases(Benign lesion 40cases, Neoplasm 22cases and Malignant lesion 41cases) through a Hematoxylin-eosin and Papanicolaou stain from cytomorphologic change by nodular lesions was observed and compared. The results were as follows. A papillary structure(88%) and psammoma body(24%) were observed in papillary carcinoma, and intranuclear pseudoinclusion was observed in papillary carcinoma(94%) and medullary carcinoma(29%), and nuclear grooves were observed in all nodular lesions(table 5). Appearance of lymphocytes was observed in all nodular lesions except hurthle cell tumor but appearance frequency was highly remarkable in grave's disease(l00%), hashimoto's thyroiditis(100%) and subacute thyroiditis(l00%). A multinucleated giant histiocyte was observed in subacute thyroiditis(l00%), papillary carcinoma(82%), adenomatous goiter(33%) and hashimoto's thyroiditis(26%) but expressed a high appearance frequency in subacute thyroiditis(Table5). A follicular structure was observed in all nodular lesions except hurthle cell tumor and sub acute thyroiditis but expressed a high appearance frequency in follicular neoplasm(l00%) and grave's disease(l00%) (Table 5). Conclusively, a papillary structure and psammoma body of cytologic features change showed papillary carcinoma in FNAC but it is considered when diagnosing papillary carcinoma because nuclear grooves and intranuclear pseudoinclusion can be observed in other nodular lesions.

갑상선의 정상과 여포상 종양 조직의 은 핵소체 형성부위(AgNOR)와 증식세포 핵 항원(PCNA)에 관한 조사

김태전,한경희 임상병리검사과학회 2001 임상병리검사과학회지 Vol.33 No.2

The histologic or cytologic distinctions between follicular adenoma and carcinoma in thyroid tissue are sometimes difficult. For the diagnosis of follicular carcinoma, invasion of blood vesseles or capsules are considered definitive criteria. However, the distinction of follicular adenoma from carcinoma may be difficult when these features are absent. Furthermore, in case with fine needle aspiration biopsy specimen, the distinction between follicular adenoma and carcinoma may also be difficult, as both are characterized by follicular form cells. The authors performed the sliver nucleolar organizer region(AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen(PCNA) on 5 samples of normal thyroid tissue, 8 follicular adenomas, and 10 carcinomas to evaluate whether these techniques can aid in these differential diagnosis. Significant differences were found in the mean AgNOR count between normal thyroid tissue (1.29±0.65) and follicular adenomas (2.88±1.33) (P<0.000l), but not between follicular adenomas and carcinomas(2.71±1.14). In the normal thyroid tissue, AgNORs were smooth and round; in the follicular adenomas and carcinomas, however, they varied in size and shape. Compound AgNORs were commonly present in the tumors but were absent in normals. Antibody to PCNA did not stain normal thyroid tissue cells but stained many follicular adenomas and carcinoma cells. These results suggested that the AgNOR technique and PCNA immunohistochemistry could be used to distinguish normal thyroid tissue form follicular adenoma, but not distinguish follicular adenoma from carcinoma.

산화질소 억제가 혈압, 심박동수 및 체중에 미치는 영향

배형준 임상병리검사과학회 1999 임상병리검사과학회지 Vol.31 No.2

This study examined the physiological and histological changes in Sprague-Dawley rats with induced hypertension by chronic treatment of NG-nitro-L-arginine methyl ester(L-NAME), nitric oxide synthesis inhibitor. Systolic blood pressure of L-NAME treatement experimental group was significantly higher compared with untreated control group by 28.5%(44.2mmHg)(p<0.001). Changing rates on systolic blood pressure of L-NAME treated groups according to during L-NAME treatment period were significantly higher than control groups by 16.1% in 2weeks, 15.1% in 4weeks(p<0.05), 29.1% in 6weeks(p<0.01) and 28.5%(p<0.001). However, heart rate and body weight did all not significant differ between L-NAME treated group and control group, between L-NAME posttreated rats and pretreated rats. Systolic blood pressure had each low correlation with heart rate and body weight. In conclusion, chronic inhibition of nitric oxide by L-NAME treatment had significant effect on systolic blood pressure, but not affect on heart rate and body weight. Thus, this study suggested that nitric oxide had important role to control for blood pressure and to modulation for function of organs associated with blood pressure.

TMT에 의한 운동부하 수행능력에 관한 연구

김종경,배형준 임상병리검사과학회 1997 임상병리검사과학회지 Vol.29 No.1

The total positive rate was 24.3% by treadmill exercise test(TMT) for adult patient 300 cases be questionable as coronary artery disease in Dept. of Cardiovascular Center, INHA University Hospital between December 1996 to March 1997. It was no significantly differ between male and female, however that was significantly differ as increase of age. The average maximum workload level and average maximum heart rate were significantly decreased more be questionable as coronary artery disease. But the average maximum systolic blood pressure, the reaching level of target maximum heart rate and the stop reasons of TMT were no significantly differ among result groups. In the positive group, the most frequent period of performance degree of maximum workload was stage 4, and also the most frequent period of depressed change of ST segment was stage 4. Futher, in the positive group, depressed cases of ST segment were 60 cases, the most frequent degree of depressed change of ST segment was higher than 1mm under the baseline.

한국여성의 요중 아초산염과 백혈구 에스터라제의 활성 및 세균뇨에 대한 평가

박정오 임상병리검사과학회 2002 임상병리검사과학회지 Vol.34 No.2

The study evaluated the usefulness of the leukocyte esterase and nitrite reagent strip tests in a general hospital as a screening test to detect significant urinary tract Infections in urine of Korean women and correlated the findings with bacterial culture, leukocyte esterase and nitrite results. A total of 689 urine samples were reviewed. 254 positive cultures (36.8%) were found, of which 190 (74.8%) were detected by leukocyte esterase-nitrite test. In addition, 357 of 372 (96%) urine samples with negative bacterial cultures were negative in leukocyte esterase and nitrite tests. The results of studies was the sensitivity (74.8%), specificity (95.2%), positive predictive value (92.6%), and negative predictive value was still 84.7% and was comparable to that found in previous studies.

Interpretation of Infrared Spectra of Renal Calculi with Fourier Transform Infrared Spectroscopy

박정오 임상병리검사과학회 2001 임상병리검사과학회지 Vol.33 No.2

신장결석 환자 2예를 대상으로 하여 체외 충격파 쇄석술 후 요 중에 존재하는 결석 성분의 FT-IR spectrum을 Sweet의 Flow chart법을 이용하여 분석한 결과, 환자 A의 경우에는 oxalate와 urate를 그리고 환자 B의 경우에는 phosphate를 주성분으로 확인할 수 있었다. 이 방법은 신속하고 정확한 분석을 재현성 있게 감별할 수 있어서 임상 검사실에서 그 유용성이 클 것으로 사료된다. For objective qualitative analysis of the IR spectra of renal calculi, We introduced systematic schemes devised by R V. Sweet for Two case of patients. We could obtained the renal calculi samples by method of Extracorporeal Shock Wave Lithotripsy. In the case of patients A, the calculi sample contains oxalate and urate and in the case of patient B, it was phosphate as a main components. The method requires less time than standard-comparing method and it's considered very useful method for clinical pathology field.