Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells

Tao Zhang,Qiang Fu,Ning-Ning Tang,Qian Zhang,Yi Liu,Jia-Chen Peng,Ning Fang,Li-Mei Yu,Jin-Wei Liu 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.4

Purpose: To explore the value of transplanting peripheral blood-derived mesenchymal stem cells from allogenic rabbits (rPBMSCs)to treat osteonecrosis of the femoral head (ONFH). Materials and Methods: rPBMSCs were separated/cultured from peripheral blood after granulocyte colony-stimulating factor mobilization. Afterwards, mobilized rPBMSCs from a second passage labeled with PKH26 were transplanted into rabbit ONFH models, which were established by liquid nitrogen freezing, to observe the effect of rPBMSCs on ONFH repair. Then, the mRNA expressions of BMP-2 and PPAR-γ in the femoral head were assessed by RT-PCR. Results: After mobilization, the cultured rPBMSCs expressed mesenchymal markers of CD90, CD44, CD29, and CD105, but failed to express CD45, CD14, and CD34. The colony forming efficiency of mobilized rPBMSCs ranged from 2.8 to 10.8 per million peripheralmononuclear cells. After local transplantation, survival of the engrafted cells reached at least 8 weeks. Therein, BMP-2 was up-regulated, while PPAR-γ mRNA was down-regulated. Additionally, bone density and bone trabeculae tended to increase gradually. Conclusion: We confirmed that local transplantation of rPBMSCs benefits ONFH treatment and that the beneficial effects are relatedto the up-regulation of BMP-2 expression and the down-regulation of PPAR-γ expression.

Salt template-assisted construction of three-dimensional interconnected network in thermally conductive EP/PVDF/NiCo@GNP composites

Ning Jia,Bin Yang,Xiaohong Wang,Shishuang Cui,Peng Chen,Ru Xia,Zhengzhi Zheng,Jiasheng Qian,Yuchao Ke,Yang Pan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

In this study, graphene nanoplatelets (GNP) and nickel–cobalt (NiCo) alloy were adopted as reinforcingmaterials to prepare epoxy (EP) resin-based composites. NiCo particles were able to achieve uniformanchoring on GNPs’ surface under the electrostatic force due to the successful introduction of oxygendefects on GNPs. With the synergistic effect of adhesive PVDF and salt particles, the system with threedimensionally(3D) interconnected network was successfully constructed. Benefit from the formation ofmechanically stable and efficient heat conduction paths, the thermal conductivity (TC) of the EP/PVDF/NiCo@GNP composite reached 1.077 Wm1K1 at a filler volume fraction of 6.82 vol%, while the counterpart’sstoragemodulus (E’) was up to 3.22 GPa. In addition, a scaling formula was quantitatively proposed tocorrelate TC with E’, and the perfect agreement between the theoretical prediction and experimental dataclarified the underlying mechanism of the relationship between phonons transport and stress transferwithin a systemwith 3D ordered structures. The establishment of relationship between AHR (average heatingrate) and TC made it possible to further predict TC of composites based upon the infrared thermal imaging(ITI) data. The as-prepared GNP composites show good application prospects as thermal managementmaterials which need to experience thermally mechanical deformation.

Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA

Ning Xue,Jian-Hua Lin,Shan Xing,Dan Liu,Shi-Bing Li,Yan-Zhen Lai,Xue-Ping Wang,Min-Jie Mao,Qian Zhong,Mu-Sheng Zeng,Wan-Li Liu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1

Purpose The purpose of this study was to identify novel plasma biomarkers for distinguishing nasopharyngeal carcinoma (NPC) patients from healthy individuals who have positive Epstein-Barr virus (EBV) viral capsid antigen (VCA-IgA). Materials and Methods One hundred seventy-four plasma cytokines were analyzed by a Cytokine Array in eight healthy individuals with positive EBV VCA-IgA and eight patients with NPC. Real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were employed to detect the expression levels of macrophage migration inhibitory factor (MIF) and CC chemokine ligand 3 (CCL3) in NPC cell lines and tumor tissues. Plasma MIF and CCL3 were measured by ELISA in 138 NPC patients, 127 EBV VCA-IgA negative (VN) and 100 EBV VCA-IgA positive healthy donors (VP). Plasma EBV VCA-IgA was determined by immunoenzymatic techniques. Results Thirty-four of the 174 cytokines varied significantly between the VP and NPC group. Plasma MIF and CCL3 were significantly elevated in NPC patients compared with VN and VP. Combination of MIF and CCL3 could be used for the differential diagnosis of NPC from VN cohort (area under the curve [AUC], 0.913; sensitivity, 90.00%; specificity, 80.30%), and combination of MIF, CCL3, and VCA-IgA could be used for the differential diagnosis of NPC from VP cohort (AUC, 0.920; sensitivity, 90.00%; specificity, 84.00%), from (VN+VP) cohort (AUC, 0.961; sensitivity, 90.00%; specificity, 92.00%). Overexpressions of MIF and CCL3 were observed in NPC plasma, NPC cell lines and NPC tissues. Conclusion Plasma MIF, CCL3, and VCA-IgA combination significantly improves the diagnostic specificity of NPC in high-risk individuals.

Efficiently Engineering Cu-Based Oxide by Surface Embedding of Ce for Selective Catalytic Reduction of NO with NH3

Qian Sun,Chun Zeng,Meng-Meng Xing,Bo Chen,Dan Zhao,San-Guo Hong,Ning Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.6

Deliberately engineering oxide composites on constructing and manipulating interactive structures particularly in surface layers was highly desirable for heterogeneous catalysis. Herein, upon the redox replacement reaction between Ce(IV) precursor (Ce(NO3)2- 6)and Cu2O nanosubstrate, an attempt to directly engineer the surface structure of Cu-based substrate was performed by the Ce(IV)–Cu2O etching-embedding process, then the obtained powders were thermo-treated to get a series of Ce–O–Cu catalysts with different Ce:Cu molar ratios for NH3 selective catalytic reduction (NH3-SCR) of NO. Characterized by ICP-OES, XRD, Raman, XPS, SEM, BET, H2-TPR, NO- and NH3-TPD measurements, it was demonstrated that the Cu–O–Ce catalysts were structured as CuO matrix with an interactive surface composed by co-present Cu(I)–Cu(II) and Ce(III)–Ce(IV) species, even the introduction of Ce was confined in a quite low loading range (0.83–2.3 wt.%); such a surface exhibited the distinct synergistic effect with positively manipulated physical-chemistry properties such as active site distributions, redox features and surface reactivity compared to pure CuO and traditional Cu–Ce composite catalyst, leading to attractive catalytic performance such as ≥ 90% NO conversion with ≥ 95% N2 selectivity and the two-fold TOF enhancement versus traditional catalysts, even SO2 was present in reactant mixture on well-manipulated catalyst (Ce loading at 1.6 wt.%) These results indicated that the etching-embedding strategy illuminated in this work could be referred as a feasible method to directly engineer and construct interactive oxide composite surface for advanced application as well as current efficient Ce–O–Cu catalytic interface for heterogeneous catalysis.

A Novel Parallel Precision Stage with Large Working Range Based on Structural Parameters of Flexible Hinges

Qian Lu,Xifu Chen,Lei Zheng,Ning Xu 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.3

In order to solve the inherent contradiction of high precision and large working range in the traditional parallel positioning stage, a novel parallel precision stage with large working range based on optimized flexible hinge was studied. Firstly, a new structure parameter Ps was proposed, and its influence on the rotation stiffness was analyzed by comparing the commonly used flexible hinges. The factors that could enlarge the rotation ability were summarized, and the cylindrical flexible hinge with large working range was optimized. Then the cylindrical flexible hinge was used to constructed a novel parallel precision positioning stage, which could realize both of high resolution and large working range by taking use of the step and continuous motion of the piezoelectric actuators. The experiment results show that the resolution of translation is 0.09 μm, and the rotation resolution are 0.8 μrad, 0.9 μrad and 1.0 μrad, respectively. While the working range of the parallel stage could achieve 120 mm for translation, and 6.18°, 6.74° and 6.58°, respectively for each axis of rotation. All of the basic research contents is helpful for further study of the control laws of the compliant parallel mechanism.

Seismic Performance Experiment Involving One-Story, Two-Bay Self-control Energy Dissipation UPPC Frames under Low Cyclic Loads

Cheng Qian,Yanhe Li,Dengfeng Xie,Ning Li,Xiaoying Wen 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.7

This paper presents an experimental study of the behavior of three one-story, two-bay unbonded partially prestressed concrete (UPPC) frames, of which two frames are self-control energy dissipation UPPC frames. This study aims to use the self-control energy dissipation UPPC frame as a new frame, and its seismic performance is evaluated compared to that of existing UPPC frames. The new frame utilizes energy dissipation components to provide energy dissipation capabilities, which allow the frame to form a beamhinge mechanism under earthquake actions. The influence of the prestress release percentage on the seismic performance of the UPPC frame is experimentally examined. The test results show that the new UPPC frame displays better ductility and energy dissipation capabilities as the prestress release percentage increases, but the lateral resistance is slightly weaker than that of a common UPPC frame. Overall, the new frame displays better seismic performance than the conventional UPPC frame and can be reused after retrofitting.

Simple Filter Cake Formation Test Method Using a Geotextile instead of the Sand Stratum

Yongjin Qian,Wei Zhu,Ning Zhang,Fanlu Min,Silin Wu 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.1

Slurries and filter cakes are widely used to support the earth pressure and water pressure of an excavation surface to maintain stability. Most major projects of slurry shield need to carry out filter cake formation tests to study the availability of slurry parameters. Nevertheless, it takes considerable testing and lengthy preparation of sand to simulate a stratum, therefore this paper examines a filter cake formation test method in which a geotextile is used to replace the sand stratum. Using the geotextile as a known filter medium, the quantitative relationship is studied between the membrane and the simulated sand, to examine whether the same experimental results can be obtained as using the simulated sand. A series of filter cake formation tests were carried out on four different sands and five different geotextiles with four different slurries and then the filter membrane properties were measured to explore the potential of the geotextile alternative for filter cake formation. The results show that the infiltration process of slurry in sand and geotextile was similar, and slurry infiltration occurred both with and without filter cake. 0.8 times apparent opening size of geotextile could be used to replace the characteristic pore diameter of the sand stratum (0.8AOS = D0) for the filter cake formation tests. The filter cakes on sand stratum and the substituted geotextile had similar properties, such as thickness and water content.

Effect of Knitting Structure and Polyethylene Content on Thermalwet Comfort and Cooling Properties of Polyethylene/polyester Fabrics

Juan Qian,Ting Xie,Liqun Chen,Zhijiang Li,Ning Guo,Shaoju Fu,Peihua Zhang 한국섬유공학회 2022 Fibers and polymers Vol.23 No.11

Polyethylene is getting tremendous attention in personal thermal management textiles for summer, but there is lackof systematical coolness and thermal-wet comfort research in wearing knitting fabrics. In this work, two group of knittingstructure were designed following a sandwich structure model that consisted of a middle layer of multifilament and twoasymmetric outer layers. Nine bi-layer samples interknitted with polyester were fabricated with one face composed ofpolyester filaments and the other face built of polyethylene and polyester filaments. Textile parameters, such as knittingstructure, polyethylene fractions and distribution in the inner layer, yarn composition and relative porosity, were considered. Breathability, thermal transfer properties, moisture management performance and dynamic cooling performance wasmeasured and evaluated to investigate the thermal-wet and coolness relevant comfort properties of these fabrics. It was foundthat the fabrics knitted with tuck stitches have better air permeability, contact coolness, overall moisture management anddynamic cooling performance in comparison to the fabrics knitted with float stitches. The air permeability became better withthe increasing proportion of polyethylene and the decreasing ratio of tuck stitch. The effective contact area of polyethylenewith skin was beneficial to promote the transient thermal conductivity and dynamic cooling performance of fabric. The resultalso suggested that an asymmetric structure with meshes in the inner layer were beneficial to promote moisture management. Meanwhile, when the proportion of tuck stitch in inner layer was 6.25 %, the air permeability and heat transfer properties offabric were the best. Moreover, independent tests had proved that the performance of transient and steady thermalconductivity of polyethylene/polyester fabric was the best when the mass ratio of polyethylene was 49.3 % in this study.

Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)

Chang-Ning Li,Manoj-Kumar Srivastava,Qian Nong,Li-Tao Yang,Yang-Rui Li,디조이사마하나마 한국유전학회 2013 Genes & Genomics Vol.35 No.1

Abscisic acid (ABA) plays important roles in adaptive responses to various environmental stresses. The rate-limiting step in ABA biosynthesis is the oxidative cleavage of cis-epoxycarotenoids, which is catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED). In this experiment,a full-length cDNA encoding NCED gene was cloned by RT-PCR and RACE from sugarcane (Saccharum officinarum L.). The full-length of SoNCED is 2,521 bp with 1,827 bp open reading frame, encoding a peptide of 608amino acids. The calculated molecular weight of protein was 65.9 kDa with isoelectric point of 6.04. Conserved domains prediction indicated a chloroplast-targeting peptide located at N-terminus of SoNCED. Phylogenetic tree, constructed by Neighbor-Joining method indicated that SoNCED shared high identity with the NCEDs reported from other plant species. Sequence alignment revealed that the basic secondary structure including a-helices, b-strands, b-propeller and His residues coordinating catalytic sites of SoNCED were highly conserved as in the NCEDs from other plants. Tissue specific expression analysis using quantitative real-time PCR showed a significant increase in SoNCEDmRNAlevel and its correlation with O2– production rate and ABA accumulation in leaves and roots of sugarcane variety GT21 when exposed to water stress. Further, the stimulation of SoNCED mRNA level, O2– production rate and ABA content after exogenous application of ABA (100 lMol l-1) proved its involvement in pathways providing tolerance to drought stress.

Longitudinal Ultrasonic Vibration Assisted Rapid Solid Phase Bonding of 2024 Aluminum Alloy Using Ag as Interlayer for Structure Lightweight Design

Baoqun Ning,Yong Nie,Qian Wang,Yu Fu,Yue Li,Jian Han,Yingfeng Shao,Jiuchun Yan 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

This paper introduced a method of rapid solid-phase bonding Al block with Ag as the intermediate layer by longitudinalultrasonic vibration under eutectic temperature in the atmospheric environment. This method is suitable for the application ofstructural lightweight design. It was observed that Al and Ag difuse with each other in the weld seam to form an intermetallic compound Ag2Al with close hexagonal structure. The efects of diferent reaction temperatures and ultrasonic vibrationtimes on the microstructure and mechanical properties of intermetallic compound Ag2Al were discussed. The infuence ofthe efect of ultrasonic softening waves on surface oxide flm rupture and element difusion was explored. With the increaseof ultrasonic vibration time, the intermetallic compounds grew along with the interface and the thickness increased. Whenthe welding temperature was 500 °C and the ultrasonic vibration was 20 s, the highest average strength of the welded jointreaches 46.68 MPa.