Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)

Chang-Ning Li,Manoj-Kumar Srivastava,Qian Nong,Li-Tao Yang,Yang-Rui Li,디조이사마하나마 한국유전학회 2013 Genes & Genomics Vol.35 No.1

Abscisic acid (ABA) plays important roles in adaptive responses to various environmental stresses. The rate-limiting step in ABA biosynthesis is the oxidative cleavage of cis-epoxycarotenoids, which is catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED). In this experiment,a full-length cDNA encoding NCED gene was cloned by RT-PCR and RACE from sugarcane (Saccharum officinarum L.). The full-length of SoNCED is 2,521 bp with 1,827 bp open reading frame, encoding a peptide of 608amino acids. The calculated molecular weight of protein was 65.9 kDa with isoelectric point of 6.04. Conserved domains prediction indicated a chloroplast-targeting peptide located at N-terminus of SoNCED. Phylogenetic tree, constructed by Neighbor-Joining method indicated that SoNCED shared high identity with the NCEDs reported from other plant species. Sequence alignment revealed that the basic secondary structure including a-helices, b-strands, b-propeller and His residues coordinating catalytic sites of SoNCED were highly conserved as in the NCEDs from other plants. Tissue specific expression analysis using quantitative real-time PCR showed a significant increase in SoNCEDmRNAlevel and its correlation with O2– production rate and ABA accumulation in leaves and roots of sugarcane variety GT21 when exposed to water stress. Further, the stimulation of SoNCED mRNA level, O2– production rate and ABA content after exogenous application of ABA (100 lMol l-1) proved its involvement in pathways providing tolerance to drought stress.

An update of preimplantation genetic diagnosis in gene diseases, chromosomal translocation, and aneuploidy screening

Chang, Li-Jung,Chen, Shee-Uan,Tsai, Yi-Yi,Hung, Chia-Cheng,Fang, Mei-Ya,Su, Yi-Ning,Yang, Yu-Shih The Korean Society for Reproductive Medicine 2011 Clinical and Experimental Reproductive Medicine Vol.38 No.3

Preimplantation genetic diagnosis (PGD) is gradually widely used in prevention of gene diseases and chromosomal abnormalities. Much improvement has been achieved in biopsy technique and molecular diagnosis. Blastocyst biopsy can increase diagnostic accuracy and reduce allele dropout. It is cost-effective and currently plays an important role. Whole genome amplification permits subsequent individual detection of multiple gene loci and screening all 23 pairs of chromosomes. For PGD of chromosomal translocation, fluorescence $in-situ$ hybridization (FISH) is traditionally used, but with technical difficulty. Array comparative genomic hybridization (CGH) can detect translocation and 23 pairs of chromosomes that may replace FISH. Single nucleotide polymorphisms array with haplotyping can further distinguish between normal chromosomes and balanced translocation. PGD may shorten time to conceive and reduce miscarriage for patients with chromosomal translocation. PGD has a potential value for mitochondrial diseases. Preimplantation genetic haplotyping has been applied for unknown mutation sites of single gene disease. Preimplantation genetic screening (PGS) using limited FISH probes in the cleavage-stage embryo did not increase live birth rates for patients with advanced maternal age, unexplained recurrent abortions, and repeated implantation failure. Polar body and blastocyst biopsy may circumvent the problem of mosaicism. PGS using blastocyst biopsy and array CGH is encouraging and merit further studies. Cryopreservation of biopsied blastocysts instead of fresh transfer permits sufficient time for transportation and genetic analysis. Cryopreservation of embryos may avoid ovarian hyperstimulation syndrome and possible suboptimal endometrium.

Fangchinoline Inhibits Cell Proliferation Via Akt/GSK-3beta/cyclin D1 Signaling and Induces Apoptosis in MDA-MB-231 Breast Cancer Cells

Wang, Chang-Dong,Yuan, Cheng-Fu,Bu, You-Quan,Wu, Xiang-Mei,Wan, Jin-Yuan,Zhang, Li,Hu, Ning,Liu, Xian-Jun,Zu, Yong,Liu, Ge-Li,Song, Fang-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Fangchinoline (Fan) inhibits cell proliferation and induces apoptosis in several cancer cell lines. The effects of Fan on cell growth and proliferation in breast cancer cells remain to be elucidated. Here, we show that Fan inhibited cell proliferation in the MDA-MB-231 breast cancer cell line through suppression of the AKT/Gsk-3beta/cyclin D1 signaling pathway. Furthermore, Fan induced apoptosis by increasing the expression of Bax (relative to Bcl-2), active caspase 3 and cytochrome-c. Fan significantly inhibited cell proliferation of MDA-MB-231 cells in a concentration and time dependent manner as determined by MTT assay. Flow cytometry analysis demonstrated that Fan treatment of MDA-MB-231 cells resulted in cell cycle arrest at the G1 phase, which correlated with apparent downregulation of both mRNA and protein levels of both PCNA and cyclin D1. Further analysis demonstrated that Fan decreased the phosphorylation of AKT and GSK-3beta. In addition, Fan up-regulated active caspase3, cytochrome-c protein levels and the ratio of Bax/Bcl-2, accompanied by apoptosis. Taken together, these results suggest that Fan is a potential natural product for the treatment of breast cancer.

Flatness Measurement of a Mosaic Focal Plane by using a Coaxial Multispectral Laser

Liu Chang-hua,Guo Ning-Xin,Wang Jian-Li,Chen Tao,Wu Zhi-Yong,Cheng Xue 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.77 No.8

The wide-field telescope will be an important tool in the discovery and birth of new theories of astronomy in future. The detectors in this kind of telescope mostly adopt a mosaic focal plane array. The focal depth of a large-F-number optical system is very small, and the flatness of the mosaic detector should be less than half the focal depth. In this paper, a new flatness measurement and data processing method is developed. A flatness measurement platform composed of a high-precision gantry platform and a coaxial multispectral displacement meter was built. The flatness of a single detector was measured under uncooled and cooled working conditions, and data processing was conducted, in which the root mean square (RMS) and the peak–valley (PV) values of the cooled detector were 0.0017 mm and 0.0112 mm, respectively. Next, a 2 × 6 mosaic model of the focal plane with a dimension of 148.5 mm × 168.5 mm was built using a metal detector model. The measurement platform was used to measure the flatness of the mosaic focal plane. According to the measurement results, the preliminary installation and mosaic adjustment were completed. The final RMS and PV values of the mosaic focal plane are 0.009 mm and 0.0808 mm, respectively. The experimental results show that the measurement and the data processing method can accurately reflect surface information on the detectors. This suggests that the method has great potential for use in ensuring the accuracy of wide-field telescope equipment in all applicable research areas.

Efficient degradation of azo dye by dual-doped photo-enhanced Fentonlike catalysts in magnetic suspension reactor

Ting Dai,Chang Li,Ning Wang,Li Yu,Meng Zhang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-

Fenton-like catalysts have sparked enormous interest from academic to industrial fields because of theirexcellent degradability and offered a promising platform for the effective treatment of wastewater. However, it remains a grand challenge to balance their catalytic performance and corrosion resistancefor industrial application. To address this issue, we report Cr and C dual-doped photo-enhancedFenton-like catalysts (Cr, C-FeSiB) in which Cr functions as a corrosion inhibitor to improve recycling performanceand C acts as an electron transfer accelerator to raise the cycle efficiency of iron ions duringcomplex Fenton-like reactions. Moreover, we design a low energy-consumption magnetic-suspensionlikereactor in which Fenton-like catalysts can work as micro-scaled stirrers to enhance the mass transferefficiency during the Fenton-like reactions owing to their soft magnetic property.

Research Articles : Agricultural Process and Food Engineering ; Storage Characteristics of Low Temperature Grain Warehouse using Ambient Cold Air in Winter

( Xiao Feng Ning ),( He Li ),( Tae Hwan Kang ),( Chang Su Han ) 한국농업기계학회 2012 바이오시스템공학 Vol.37 No.3

Purpose: This study was conducted to investigate the storage and quality characteristics of brown rice under the low temperature warehouse conditions using ambient cold air in the winter. Methods: This new technique maintains rough rice warehouse temperature below 15℃ without cooling operation until the end of May. Four hundred tons of rough rice were stored in the low temperature grain warehouse, and were aerated from the top to the bottom using ambient cold air in February. The quality of rough rice was evaluated from February through October. Results: The results were as followings. Moisture contents of rough rice in the low temperature storage had decreased less than the ordinary temperature storage. Cracked rate of brown rice in the ordinary temperature storage and low temperature storage increased by 4~10.8% and 1.6~7.2%, respectively. The germination rate of rough rice under the ordinary and the low temperature warehouse decreased by 15.0~25.0% and 1.7~8.0%, respectively. The acid value of brown rice under the ordinary and the low temperature warehouse increased by 3.67~6.72 KOH mg/100g and 3.08~4.08 KOH mg/100g, respectively. Conclusions: The result indicates that low temperature storage using ambient winter air showed better maintaining germination of rice, less change of physiological activities and cracked kernel, and better maintaining of rice quality, comparing the ordinary temperature storage.

Anti-Inflammatory Activity of Epimedium brevicornu Maxim Ethanol Extract

Shan Huang,Ning Meng,Bingquan Chang,Xianghua Quan,RuiYing Yuan,Bin Li 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.7

Epimedium brevicornu Maxim has been used as a traditional herbal drug in China. In this study, the anti-inflammatory effects of E. brevicornu Maxim ethanol extract (EBME) were investigated in RAW264.7 macrophages and mice challenged with lipopolysaccharide (LPS). Results showed that EBME attenuated inflammation by decreasing the production of several proinflammatory mediators, such as nitric oxide (NO), prostaglandin (PG) E2, inducible nitric oxide synthase, and cyclooxygenase-2, in LPS-stimulated RAW264.7 macrophages. EBME increased the expression of heme oxygenase-1 (HO-1) and promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2. The inhibitory effects of EBME on LPS-stimulated NO and PGE2 expression were partially reversed by HO-1 inhibitor. EBME also elicited an anti-inflammatory effect by inhibiting the production of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in LPS-induced peritonitis. Therefore, EBME exhibited anti-inflammatory effects in vitro and in vivo.

Diversity of Arbuscular Mycorrhizal Fungi Associated with a Sb Accumulator Plant, Ramie (Boehmeria nivea), in an Active Sb Mining

( Yuan Wei ),( Zhi Peng Chen ),( Feng Chang Wu ),( Ji Ning Li ),( Yu Xian Shangguan ),( Fa Sheng Li ),( Qing Ru Zeng ),( Hong Hou ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8

Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this study was to investigate the colonization and molecular diversity of AMF associated with the Sb accumulator ramie (Boehmeria nivea) growing in Sb-contaminated soils. Four Sb mine spoils and one adjacent reference area were selected from Xikuangshan in southern China. PCR-DGGE was used to analyze the AMF community composition in ramie roots. Morphological identification was also used to analyze the species in the rhizosphere soil of ramie. Results obtained showed that mycorrhizal symbiosis was established successfully even in the most heavily polluted sites. From the unpolluted site Ref to the highest polluted site T4, the spore numbers and AMF diversity increased at first and then decreased. Colonization increased consistently with the increasing Sb concentrations in the soil. A total of 14 species were identified by morphological analysis. From the total number of species, 4 (29%) belonged to Glomus, 2 (14%) belonged to Acaulospora, 2 (14%) belonged to Funneliformis, 1 (7%) belonged to Claroideoglomus, 1 (7%) belonged to Gigaspora, 1 (7%) belonged to Paraglomus, 1 (7%) belonging to Rhizophagus, 1 (7%) belonging to Sclervocystis, and 1 (7%) belonged to Scutellospora. Some AMF sequences were present even in the most polluted site. Morphological identification and phylogenetic analysis both revealed that most species were affiliated with Glomus, suggesting that Glomus was the dominant genus in this AMF community. This study demonstrated that ramie associated with AMF may have great potential for remediation of Sb-contaminated soils.

Effects of Ribosomal Protein L39-L on the Drug Resistance Mechanisms of Lung Cancer A549 Cells

Liu, Hong-Sheng,Tan, Wen-Bin,Yang, Ning,Yang, Yuan-Yuan,Cheng, Peng,Liu, Li-Juan,Wang, Wei-Jie,Zhu, Chang-Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: Cancer is a major threat to the public health whether in developed or in developing countries. As the most common primary malignant tumor, the morbidity and mortality rate of lung cancer continues to rise in recent ten years worldwide. Chemotherapy is one of the main methods in the treatment of lung cancer, but this is hampered by chemotherapy drug resistance, especially MDR. As a component of the 60S large ribosomal subunit, ribosomal protein L39-L gene was reported to be expressed specifically in the human testis and human cancer samples of various tissue origins. Materials and Methods: Total RNA of cultured drug-resistant and susceptible A549 cells was isolated, and real time quantitative RT-PCR were used to indicate the transcribe difference between amycin resistant and susceptible strain of A549 cells. Viability assay were used to show the amycin resistance difference in RPL39-L transfected A549 cell line than control vector and null-transfected A549 cell line. Results: The ribosomal protein L39-L transcription level was 8.2 times higher in drug-resistant human lung cancer A549 cell line than in susceptible A549 cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells showed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions and Implications for Practice: The ribosomal protein L39-L gene may have effects on the drug resistance mechanism of lung cancer A549 cells.

Multiscale Belief Propagation on Concrete CT Image Fast Segmentation

Zhao Liang,Lu Jun,Chen Deng-feng,Li chang-hua,Dang Fa-ning 보안공학연구지원센터(IJGDC) 2013 International Journal of Grid and Distributed Comp Vol.6 No.6