Assessment of Genetic Diversity in Different Populations of Raily Ecorace of Indian Tasar Silkworm, Antheraea Mylitta Drury Using ISSR Markers

Srivastava, Ashok Kumar,Kar, Prasanta Kumar,Sinha, Ravibhushan,Sinha, Manoj Kumar,Vijayaprakash, Nanjappa Basavappa Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.19 No.2

Raily ecorace of Indian tasar silkworm is wild in nature and distributed abundantly in dense deciduous forest on Shorea robusta (Sal) in Bastar ($17^{\circ}4'$ and $20^{\circ}34'$ N, $80^{\circ}15'$ and $82^{\circ}15'$ E and altitude ranging from 150 to 1200 mMSL) forest ranges of Chhattisgarh, India. It is represented by about 20 populations. Out of those, eleven populations showed intra- as well as inter- population variability based on phenotypic expression and also in major economic traits viz. cocoon weight, shell weight, filament length and denier. Genetic diversity in these eleven populations was studied using Inter-Simple Sequence Repeat (ISSR) markers. The band profiles generated with eight ISSR primers have depicted variation in band size. All the primers exhibited polymorphism which is an indicative of the genetic variation in individual Raily silkworm. Among the populations, total polymorphism recorded was 76%. The population genetic aspects assessed through POPGENE software package are discussed in the paper. Nei's gene diversity (h) ranged from 0.194 to 0.337 exhibiting high heterozygosity. Relevance of the present study is of high significance in formulating conservation strategies and sustainable utilization of the economically important Raily ecorace of Antheraea mylitta.

Mesophase formation behavior in petroleum residues

Subhash Kumar,Manoj Srivastava 한국탄소학회 2015 Carbon Letters Vol.16 No.3

Mesophase pitch is an important starting material for making a wide spectrum of industrial and advanced carbon products. It is produced by pyrolysis of petroleum residues. In this work, mesophase formation behavior in petroleum residues was studied to prepare environmentally-benign mesophase pitches, and the composition of petroleum residues and its influence on the mesophase formation was investigated. Two petroleum residues, i.e., clarified oils (CLO-1, CLO-2) obtained from fluid catalytic cracking units of different Indian petroleum refineries, were taken as feed stocks. A third petroleum residue, aromatic extract (AE), was produced by extraction of one of the CLO-1 by using N-methyl pyrrolidone solvent. These petroleum residues were thermally treated at 380°C to examine their mesophase formation behavior. Mesophase pitches produced as a result of thermal treatment were characterized physico-chemically, as well as by instrumental techniques such as Fourier-transform infrared spectroscopy, nuclear magnetic resonance, X-ray diffraction and thermogravimetry/derivative thermogravimetry. Thermal treatment of these petroleum residues led to formation of a liquid-crystalline phase (mesophase). The mesophase formation behavior in the petroleum residues was analyzed by optical microscopy. Mesophase pitch prepared from CLO-2 exhibited the highest mesophase content (53 vol%) as compared to other mesophase pitches prepared from CLO-1 and AE.

Assessment of Genetic Diversity in Different Populations of Raily Ecorace of Indian Tasar Silkworm, Antheraea Mylitta Drury Using ISSR Markers

( Ashok Kumar Srivastava ),( Prasanta Kumar Kar ),( Ravibhushan Sinha ),( Manoj Kumar Sinha ),( Nanjappa Basavappa Vijayaprakash ) 한국잠사학회 2009 International Journal of Industrial Entomology Vol.19 No.2

Raily ecorace of Indian tasar silkworm is wild in nature and distributed abundantly in dense deciduous forest on Shorea robusta (Sal) in Bastar (17°4` and 20°34` N, 80°15` and 82°15` E and altitude ranging from 150 to 1200 mMSL) forest ranges of Chhattisgarh, India. It is represented by about 20 populations. Out of those, eleven populations showed intra- as well as inter- population variability based on phenotypic expression and also in major economic traits viz. cocoon weight, shell weight, filament length and denier. Genetic diversity in these eleven populations was studied using Inter-Simple Sequence Repeat (ISSR) markers. The band profiles generated with eight ISSR primers have depicted variation in band size. All the primers exhibited polymorphism which is an indicative of the genetic variation in individual Raily silkworm. Among the populations, total polymorphism recorded was 76%. The population genetic aspects assessed through POPGENE software package are discussed in the paper. Nei`s gene diversity (h) ranged from 0.194 to 0.337 exhibiting high heterozygosity. Relevance of the present study is of high significance in formulating conservation strategies and sustainable utilization of the economically important Raily ecorace of Antheraea mylitta.

Cyclin D1, Retinoblastoma and p16 Protein Expression in Carcinoma of the Gallbladder

Srivastava, Vineeta,Patel, Brijesh,Kumar, Mohan,Shukla, Mridula,Pandey, Manoj Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Background: Cancer of the gallbladder is a relatively rare neoplasm with a poor prognosis. The exact mechanisms of its genesis are not known and very little information is available on molecular events leading to labeling this as an orphan cancer. Materials and Methods: In this prospective case control study we evaluated the expression of p16, pRb and cyclin D1 by immunohistochemistry to study the G1-S cell-cycle check point and its possible role in gallbladder carcinogenesis. A total of 25 patients with gallbladder carcinoma (group I), 25 with cholelithiasis (group II) and 10 normal controls. were enrolled Results: Cyclin D1 expression was seen in 10 (40%) patients each with carcinoma and cholelithiasis while only in 2 (20%) of the normal gallbladders but differences were not statistically significant (p value=0.488). p16 was expressed in 12% patients of carcinoma of the gallbladder and 28% of cholelithiasis, however this difference was not statistically significant (p value=0.095). Retinoblastoma protein was found to be expressed in 50% of normal gallbladders and 6 (24%) of carcinoma and 8 (32%) of gallstones. The present study failed to demonstrate any conclusive role of cyclin D1/RB/ p16 pathway in carcinoma of the gallbladder. Conclusions: The positive relation observed between tumor metastasis and cyclinD1 expression and p16 with nodal metastasis suggested that higher cyclin D1/p16 expression may act as a predictive biomarker for aggressive behavior of gallbladder malignancies.

Rheological studies of performance grade bitumens prepared by blending elastomeric SBS (styrene butadiene styrene) co-polymer in base bitumens

Kamal Kumar,Anand Singh,Samir K. Maity,Manoj Srivastava,Manisha Sahai,Raj K. Singh,Madhukar O. Garg 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.44 No.-

Performance of bituminous binder in terms of viscoelastic behavior can be improved by addition ofrequisite polymers. Two performance grade bituminous binder PG58 and PG64 were prepared byblending an elastomeric styrene butadiene styrene (SBS) co-polymer in 60/70 and 80/100 grade basebitumen respectively. The rheological properties of prepared bitumens have been studied by AR 1500 eXAsphalt Rheometer at minimum instrument inertia of 16.85 mNm2. Resistance to permanentdeformation or deformation resistance is also determined in terms of G0modified bitumen/G0base bitumen,G00modified bitumen/G00base bitumen and G*modified bitumen /G*base bitumen; which helps to predict the viscoelasticbehavior of bituminous binder.

Influence of Inhibitors on the Corrosion of Al and Al-composites in Chloride-containing Solutions - A Review

Neeraj Kumar,Ashok K. Srivastava,Prabhat Gautam,M. K. Manoj 한국재료학회 2022 한국재료학회지 Vol.32 No.5

Corrosion is a natural, inevitable process, and is one of the world's most serious problems. Losses incurred due to corrosion are extremely expensive for society. Several technological strategies have been explored and implemented to address these losses. The use of inhibitors to prevent corrosion is a common and efficient method to reduce corrosion losses. This review covers Al and Al-composite corrosion inhibitors in chloride-containing solutions, because of their popularity in a broad array of industrial applications. A vast number of studies in the literature detail the common tendency of Al and Al-composites with reinforcements to deteriorate. Accordingly, it is worthwhile to employ inhibitors to protect them, as discussed in the present work. The emphasis is on selecting the smartest corrosion inhibitor and evaluating its performance. According to the study, the most commonly used corrosion inhibitors are 1,4-naphthoquinone (NQ), 1,5-naphthalene diol, 3-amino-1,2,4-triazole-5-thiol (ATAT), ammonium tetrathiotungstate, clotrimazole, amoxicillin, antimicrobial and antifungal drugs. Electrochemical impedance spectroscopy (EIS), potentiodynamic (PDP), and weight loss were among the most commonly used modern electrochemical technologies to test inhibitors’ efficacy under environmental conditions.

Meliorate optical textures and mesophase contents by promising approach of deasphalting of petroleum residues

Subhash Kumar,Manoj Srivastava 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-

The aim of this work is to study melioration in mesophase formation behaviour by deasphalting ofpetroleum residues. Pitches prepared from VRs have undesirable high values of physico-chemicalproperties as compared to their corresponding DAOs pitches. This is due to presence of high molecularweight asphaltenes molecules in VRs. The optical microscopic images of VR-390-1 and VR-390-2 pitchesshowed that they have small sized, distorted and agglomerated types of mesophase having mesophasecontents (MC) 25 vol% and 22 vol% respectively but deasphalting of these VRs improve the growth andoptical texture of mesophase in DAO-390-1 (29 vol%) and DAO-390-2 (35 vol%).

Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)

Chang-Ning Li,Manoj-Kumar Srivastava,Qian Nong,Li-Tao Yang,Yang-Rui Li,디조이사마하나마 한국유전학회 2013 Genes & Genomics Vol.35 No.1

Abscisic acid (ABA) plays important roles in adaptive responses to various environmental stresses. The rate-limiting step in ABA biosynthesis is the oxidative cleavage of cis-epoxycarotenoids, which is catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED). In this experiment,a full-length cDNA encoding NCED gene was cloned by RT-PCR and RACE from sugarcane (Saccharum officinarum L.). The full-length of SoNCED is 2,521 bp with 1,827 bp open reading frame, encoding a peptide of 608amino acids. The calculated molecular weight of protein was 65.9 kDa with isoelectric point of 6.04. Conserved domains prediction indicated a chloroplast-targeting peptide located at N-terminus of SoNCED. Phylogenetic tree, constructed by Neighbor-Joining method indicated that SoNCED shared high identity with the NCEDs reported from other plant species. Sequence alignment revealed that the basic secondary structure including a-helices, b-strands, b-propeller and His residues coordinating catalytic sites of SoNCED were highly conserved as in the NCEDs from other plants. Tissue specific expression analysis using quantitative real-time PCR showed a significant increase in SoNCEDmRNAlevel and its correlation with O2– production rate and ABA accumulation in leaves and roots of sugarcane variety GT21 when exposed to water stress. Further, the stimulation of SoNCED mRNA level, O2– production rate and ABA content after exogenous application of ABA (100 lMol l-1) proved its involvement in pathways providing tolerance to drought stress.

Biochemical Performance and Quantitative Assessment of F1 Hybrid of Two Ecoraces of Tropical Tasar Silkworm Antheraea Mylitta Drury (Lepidoptera: Saturniidae)

( Gangadharaiah Lokesh ),( Sushma Rani Tirkey ),( Ashok Kumar Srivastava ),( Prasant Kumar Kar ),( Manoj Kumar Sinha ) 한국잠사학회 2013 International Journal of Industrial Entomology Vol.26 No.2

Antheraea mylitta Drury is basically a crossbreeding species, as such it seems to be potentially a good material for the exploitation of heterosis. In the present study F1 hybrid of wild ecorace Laria (L) and semi-domestic Daba (D) was raised and evaluated for various quantitative traits and biochemical parameters during larval stage. Improved fecundity (+18 ± 1.8%) and higher egg hatching rate (+10.96 ± 1.3%) was recorded in the F1hybrid (L x D). Biochemical parameters studied in the hemolymph, midgut and fatbody of the larva showed significantly higher (P<0.05) total proteins and carbohydrate concentration besides digestive enzyme activity. Correspondingly SDS-PAGE revealed more number of protein bands in the hemolymph sample of F1s, ranging between 29 kDa to 66 kDa compared to parental lines. The present study demonstrates the positive heterosis effect in the F1 hybrid of Laria x Daba. Biochemical analysis indicates that, there is possibilities of exploitation of hybrids with specific parents targeted for desirable commercial traits (silk yield and fecundity). Moreover, most of these biochemical parameters can be used as markers to analyze the genetic improvement in the tasar silkworms.

Preparation, characterization and application of polyaniline nanospheres to biosensing

Dhand, Chetna,Das, Maumita,Sumana, Gajjala,Srivastava, Avanish Kumar,Pandey, Manoj Kumar,Kim, Cheol Gi,Datta, Monika,Malhotra, Bansi Dhar Royal Society of Chemistry 2010 Nanoscale Vol.2 No.5

<P>Polyaniline nanospheres (PANI-NS) prepared by morphological transformation of micelle polymerized camphorsulfonic acid (CSA) doped polyaniline nanotubes (PANI-NT) in the presence of ethylene glycol (EG) have been characterized by X-ray diffraction, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, Fourier transform infra-red and UV-Visible spectroscopy. A PANI-NS (60–80 nm) film deposited onto an indium-tin-oxide (ITO) coated glass plate by the solution casting method has been utilized for covalent immobilization of biomolecules (cholesterol oxidase (ChOx)) <I>via</I><I>N</I>-ethyl-<I>N</I>′-(3-dimethylaminopropyl) carbodiimide (EDC) and <I>N</I>-hydroxysuccinimide (NHS) chemistry for fabrication of a cholesterol biosensor. The ChOx/PANI-NS/ITO bioelectrode detects cholesterol in the concentration range of 25 to 500 mg dL<SUP>−1</SUP> with sensitivity of 1.3 × 10<SUP>−3</SUP> mA mg<SUP>−1</SUP> dL and regression coefficient of 0.98. Further, this PANI-NS based bioelectrode shows fast response time (10 s), low Michaelis–Menten constant (2.5 mM) and shelf-life of 12 weeks. The spherical nanostructure observed in the final morphology of the PANI-NS film is attributed to hydrogen bonding interactions between PANI-NT and EG.</P> <P>Graphic Abstract</P><P>Morphological transformation of CSA-PANI nanotubes to nanospheres in EG <I>via</I> solvation energy generated from hydrogen bonding interactions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b9nr00346k'> </P>