piRNA-1742 promotes renal cell carcinoma malignancy by regulating USP8 stability through binding to hnRNPU and thereby inhibiting MUC12 ubiquitination

Zhang Wentao,Zheng Zongtai,Wang Keyi,Mao Weipu,Li Xue,Wang Guangchun,Zhang Yuanyuan,Huang Jianhua,Zhang Ning,Wu Pengfei,Liu Ji,Zhang Haimin,Che Jianping,Peng Bo,Zheng Junhua,Li Wei,Yao Xudong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Accumulating studies have confirmed that PIWI-interacting RNAs (piRNAs) are considered epigenetic effectors in cancer. We performed piRNA microarray expression analysis on renal cell carcinoma (RCC) tumor tissues and paired normal tissues and performed a series of in vivo and in vitro experiments to explore piRNAs associated with RCC progression and investigate their functional mechanisms. We found that piR-1742 was highly expressed in RCC tumors and that patients with high piR-1742 expression had a poor prognosis. Inhibition of piR-1742 significantly reduced tumor growth in RCC xenograft and organoid models. Mechanistically, piRNA-1742 regulates the stability of USP8 mRNA by binding directly to hnRNPU, which acts as a deubiquitinating enzyme that inhibits the ubiquitination of MUC12 and promotes the development of malignant RCC. Subsequently, nanotherapeutic systems loaded with piRNA-1742 inhibitors were found to effectively inhibit the metastasis and growth of RCC in vivo. Therefore, this study highlights the functional importance of piRNA-related ubiquitination in RCC and demonstrates the development of a related nanotherapeutic system, possibly contributing to the development of therapeutic approaches for RCC.

Pedestrian Choices of Vertical Walking Facilities inside Urban Rail Transit Stations

Ning Zhang,Yunlong Zhang,Xiaojun Zhang 대한토목학회 2015 KSCE JOURNAL OF CIVIL ENGINEERING Vol.19 No.3

This paper investigates pedestrians’ choices between escalator and stairway inside urban rail transit stations during peak hours. After the review of mode choice models, a logit model based on the utility maximization theory is selected. Binary logit models forthe passenger flow distribution are established with walking time, walking distance, gender and age of the passenger as the maininfluencing factors. The influences of these factors are quantitatively analyzed through data collected from three rail transit stationswith different arrangement forms of vertical walking facilities in Nanjing, China. The developed models are verified to produce ahigh accuracy in predicting passengers’ choices of vertical walking facilities. With developed models, sensitivity analyses forselecting escalator for upward and downward pedestrians in the three types of stations are also conducted and compared to furtherreveal the differences in pedestrians' choices of using vertical walking facilities. The modeling procedure and results can provide atheoretical reference for operation decisions, such as the planning of vertical walking facilities and passenger flow organization inbusy rail transit stations.

Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China

Yan Zhang,Yali Lv,Feifei Zhang,Wenjing Zhang,Jinhong Wang,Yanyan Cui,Rongjun Wang,Fuchun Jian,Longxian Zhang,Changshen Ning 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4

Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction(PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targetingthe 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710)of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621,9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheepand 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasmaspecies in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as arelationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand theepidemiology of anaplasmosis in ruminants from China.

Design of a Light and Small Dual-band Airborne Despun Optical System

Luqing Zhang,Ning Zhang,Xiping Xu,Kailin Zhang,Yue Zhang,Jiachong Li 한국광학회 2024 Current Optics and Photonics Vol.8 No.1

In aerial cameras, image quality is easily affected by weather, temperature, and the attitude of the aircraft. Aiming at this phenomenon, based on the theory of two-step zoom optical systems, a dual-band optical-despun two-step zoom optical system is designed. The system has a small field of view of 2.00° × 1.60°, and a large field of view of 4.00° × 3.20°. In the zoom process, the wavelength range is 0.45– 0.70 μm and 0.75–1.10 μm, and the size of the optical system is 168 mm (L) × 90 mm (W) × 60 mm (H). The overall lens weight is only 170.8 g, which has advantages for miniaturization and light weight. At the Nyquist frequency of 104 lp/mm, the modulation transfer function of the visible-light optical system is more than 0.44, and that of the near-infrared optical system is more than 0.30, both of which havegood imaging quality and tolerance characteristics in the range of −45 to 60 ℃.

Preclinical Study of Cell Therapy for Osteonecrosis of the Femoral Head with Allogenic Peripheral Blood-Derived Mesenchymal Stem Cells

Tao Zhang,Qiang Fu,Ning-Ning Tang,Qian Zhang,Yi Liu,Jia-Chen Peng,Ning Fang,Li-Mei Yu,Jin-Wei Liu 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.4

Purpose: To explore the value of transplanting peripheral blood-derived mesenchymal stem cells from allogenic rabbits (rPBMSCs)to treat osteonecrosis of the femoral head (ONFH). Materials and Methods: rPBMSCs were separated/cultured from peripheral blood after granulocyte colony-stimulating factor mobilization. Afterwards, mobilized rPBMSCs from a second passage labeled with PKH26 were transplanted into rabbit ONFH models, which were established by liquid nitrogen freezing, to observe the effect of rPBMSCs on ONFH repair. Then, the mRNA expressions of BMP-2 and PPAR-γ in the femoral head were assessed by RT-PCR. Results: After mobilization, the cultured rPBMSCs expressed mesenchymal markers of CD90, CD44, CD29, and CD105, but failed to express CD45, CD14, and CD34. The colony forming efficiency of mobilized rPBMSCs ranged from 2.8 to 10.8 per million peripheralmononuclear cells. After local transplantation, survival of the engrafted cells reached at least 8 weeks. Therein, BMP-2 was up-regulated, while PPAR-γ mRNA was down-regulated. Additionally, bone density and bone trabeculae tended to increase gradually. Conclusion: We confirmed that local transplantation of rPBMSCs benefits ONFH treatment and that the beneficial effects are relatedto the up-regulation of BMP-2 expression and the down-regulation of PPAR-γ expression.

A Study on the Art Management and Cultural Activities in China from the K-Wave Case

Jia Ning LI(Jia Ning LI),Su SHUAI(Su SHUAI ),Zhang FAN(Zhang FAN ) 한국한류학회 2023 한류연구 Vol.2 No.1

The purpose of this paper is to study the relationship between Art management and Cultural activities in China from the K-wave case. Research design, data, and methodology: This study used the method of the cases study survey, with the K-wave case in as the survey objects, 3 valid survey sample case were collected in this paper. Results: The two dimensions of art management and cultural activities differently influences from the K-wave, and the influence of availability on customer satisfaction is not significant. Conclusion: The results of this study will provide useful reference for the Art management and Cultural activities in China from the K-wave case. And improve the development of art management and cultural activities in China by improving the K-wave of service quality. Art management can shape urban commercial space in a beneficial way, create a good consumption atmosphere, and enhance the attractiveness and competitiveness of the city. The introduction of art elements into urban commercial space can create a strong cultural atmosphere, so that the city presents a smart look. Art management has a positive impact on K-Wave production and operation activities, which can promote the shaping of art commercial space, the promotion of urban cultural atmosphere.

Numerical method study of how buildings affect the flow characteristics of an urban canopy

Ning Zhang,Weimei Jiang,Fei Hu 한국풍공학회 2004 Wind and Structures, An International Journal (WAS Vol.7 No.3

Molecular Dynamics Investigation of the Effects of Concentration on Hydrogen Bonding in Aqueous Solutions of Methanol, Ethylene Glycol and Glycerol

Ning Zhang,Weizhong Li,Cong Chen,Jianguo Zuo,Lindong Weng 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

Hydrogen bonding interaction between alcohols and water molecules is an important characteristic in the aqueous solutions of alcohols. In this paper, a series of molecular dynamics simulations have been performed to investigate the aqueous solutions of low molecular weight alcohols (methanol, ethylene glycol and glycerol) at the concentrations covering a broad range from 1 to 90 mol %. The work focuses on studying the effect of the alcohols molecules on the hydrogen bonding of water molecules in binary mixtures. By analyzing the hydrogen bonding ability of the hydroxyl (-OH) groups for the three alcohols, it is found that the hydroxyl group of methanol prefers to form more hydrogen bonds than that of ethylene glycol and glycerol due to the intra-and intermolecular effects. It is also shown that concentration has significant effect on the ability of alcohol molecule to hydrogen bond water molecules. Understanding the hydrogen bonding characteristics of the aqueous solutions is helpful to reveal the cryoprotective mechanisms of methanol, ethylene glycol and glycerol in aqueous solutions.

Enhanced drought and salinity tolerance in transgenic potato plants with a BADH gene from spinach

Zhang, Ning,Si, Huai-Jun,Wen, Gang,Du, Hong-Hui,Liu, Bai-Lin,Wang, Di The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.1

Drought and salinity are the most important abiotic stresses that affect the normal growth and development of plants. Glycine betaine is one of the most important osmolytes present in higher plants that enable them to cope with environmental stresses through osmotic adjustment. In this study, a betaine aldehyde dehydrogenase (BADH) gene from spinach under the control of the stress-induced promoter rd29A from Arabidopsis thaliana was introduced into potato cultivar Gannongshu 2 by the Agrobacterium tumefaciens system. Putative transgenic plants were confirmed by Southern blot analysis. Northern hybridization analysis demonstrated that expression of BADH gene was induced by drought and NaCl stress in the transgenic potato plants. The BADH activity in the transgenic potato plants was between 10.8 and 11.7 U. There was a negative relationship (y = -2.2083x + 43.329, r = 0.9495) between BADH activity and the relative electrical conductivity of the transgenic potato plant leaves. Plant height increased by 0.4-0.9 cm and fresh weight per plant increased by 17-29% for the transgenic potato plants under NaCl and polyethylene glycol stresses compared with the control potato plants. These results indicated that the ability of transgenic plants to tolerate drought and salt was increased when their BADH activity was increased.

S-I model of horizontal jet grouting reinforcement for soft soil

Zhang, Ning,Li, Zhongyin,Ma, Qingsong,Ma, Tianchi,Niu, Xiaodong,Liu, Xixi,Feng, Tao Techno-Press 2018 Geomechanics & engineering Vol.15 No.5

A superposition-iteration (S-I) model is proposed to simulate the jet grouting pre-reinforcing impact for a shallow-buried tunnel. The common model is deduced by theoretical (force equilibrium) analysis and then transformed into the numerical formulation. After applying it to an actual engineering problem, the most obvious deficiency was found to be continuous error accumulation, even when the parameters change slightly. In order to address this problem, a superposition-iteration model is developed based on the basic assumption and superposition theory. First, the additional deflection between two successive excavation steps is determined. This is caused by the disappearance of the supporting force in the excavated zone and the soil pressure in the disturbed zone. Consequently, the final deflection can be obtained by repeatedly superposing the additional deflection to the initial deflection in the previous steps. The analytical solution is then determined with the boundary conditions. The superposition-iteration model is thus established. This model was then applied and found to be suitable for real-life engineering applications. During the calculation, the error induced by the ill-conditioned problem of the matrix is easily addressed. The precision of this model is greater compared to previous models. The sensitivity factors and their impact are determined through this superposition-iteration model.