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( Hongming Song ),( Dengfeng Li ),( Tianqi Wu ),( Dan Xie ),( Kaiyao Hua ),( Jiashu Hu ),( Xiaochong Deng ),( Changle Ji ),( Yijun Deng ),( Lin Fang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.11
Aberrant expression of microRNAs (miRNAs) plays important roles in carcinogenesis and tumor progression. However, the expression and biological role of miR-301b in triple-negative breast cancer (TNBC) remains unclear. Here we aimed to evaluate the roles and mechanisms of miR-301b in TNBC cells. miR-301b expression was assessed in TNBC specimens and cell lines by quantitative Real-Time PCR (qRT-PCR). TNBC cells were transfected with miR-301b mimics, inhibitors or Cylindromatosis (CYLD) small interfering RNA (siRNA) using Lipofectamine 2000. The functional roles of miR-301b were determined by cell proliferation, colony formation, and apoptosis assays. Western blots and qRT-PCR were used to measure the expression of mRNAs and proteins in the cells. We found that miR-301b was upregulated in TNBC specimens and cell lines. Overexpression of miR-301b promoted cell proliferation in TNBC cells, while inhibited the apoptosis induced by 5-FU. CYLD was downregulated by miR-301b at both mRNA and protein levels in TNBC cells. Dual-luciferase report assay confirmed that miR-301b downregulated CYLD by direct interaction with the 3’-untranslated region(3’-UTR) of CYLD mRNA. NF-κB activation was mechanistically associated with miR-301b-mediated downregulation of CYLD. However, inhibition of miR-301b reversed all the effects of miR-301b. In conclusion, miR-301b plays an oncogenic role in TNBC possibly by downregulating CYLD and subsequently activating NF-κB p65, and this may provide a novel therapeutic approach for TNBC. [BMB Reports 2018; 51(11): 602-607]
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Cheng Qian,Yanhe Li,Dengfeng Xie,Ning Li,Xiaoying Wen 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.7
This paper presents an experimental study of the behavior of three one-story, two-bay unbonded partially prestressed concrete (UPPC) frames, of which two frames are self-control energy dissipation UPPC frames. This study aims to use the self-control energy dissipation UPPC frame as a new frame, and its seismic performance is evaluated compared to that of existing UPPC frames. The new frame utilizes energy dissipation components to provide energy dissipation capabilities, which allow the frame to form a beamhinge mechanism under earthquake actions. The influence of the prestress release percentage on the seismic performance of the UPPC frame is experimentally examined. The test results show that the new UPPC frame displays better ductility and energy dissipation capabilities as the prestress release percentage increases, but the lateral resistance is slightly weaker than that of a common UPPC frame. Overall, the new frame displays better seismic performance than the conventional UPPC frame and can be reused after retrofitting.
ScienceON
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