Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

이미지즘의 동양 시학적 가능성 고찰 : 언어관과 자연관을 중심으로

전미정 우리말글학회 2003 우리말 글 Vol.28 No.-

Imagism is not to proceeds from Western but from Oriental poetry. But studies about Imagism in Korean modern poetry have argued that it is a mode derived from influence of England and America. It not good to study like that. The aim of this paper is to compare Jung Ji-yong' versification with Ezra Pound'. Similarity as they are, they assentially have a different property. The different property itself shows all importence, which is Oriental property of Jung'poem. This paper will about to expound Oriental versification through Lee Kyu-bo' versification. Accurately, through Lee' view of word and nature. Because concretly a view of word and mature reflects Oriental poetic.

한국비서자격시험의 개선점과 비교연구

남자현,이미정,이미영,이아영,정해금,최유정,이미숙,전인선,정혜림 이화여자대학교 법정대학 비서학과 1997 비서학연구 Vol.- No.17

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

부산지역 매출액 상위기업의 위상과 변천사

정성문 · 정지미 · 이규용 · 김건우 · 김권호 · 박수정 · 양태완 · 지준영 · 이우평 신라대학교 부산학연구센터 2020 부산연구 Vol.18 No.2

본 연구는 부산지역의 주요기업 변화를 살펴보기 위해 매출액 기준 전국 상위 1,000대 기업에 속하는 부산기업을 조사하여 비교분석을 실시하였다. 분석결과 2005년에 전국 1,000대 기업 중 46개 기업이 부산에 소재하였는데, 2019년에는 12 개가 줄어든 34개 기업만이 전국 1,000대 기업에 포함되었다. 또한, 전국 상위 1,000대 기업의 매출액 기준으로 부산지역 기업의 매출액 비중은 2005년 1.6%에서 2019년 1.4%로 줄어든 것으 로 나타났다. 이는 수도권 집중현상이 점차 강화되고 있기 때문이다. 한편, 부산지역 매출액 기준 상위 10대 기업은 많은 변화가 있었는데, 2005년 상위 10대 기업 중 2019년에도 상위 10대 기 업에 포함된 기업은 ㈜한진중공업, 르노삼성자동차(주), ㈜부산은행, ㈜서원유통 등 4개뿐인 것 으로 나타났다. 통계적 인과 관계는 확정할 수 없으나 지역 대기업의 쇠퇴 및 유출과 지역의 쇠퇴가 동시에 이루어지고 있어 지역 경제의 침체와 대기업의 쇠퇴 및 유출이 상당한 관계가 있음을 유추해 볼 수 있다. 특히 2011년 이후 지역 주력 제조업 기업들이 1000대 기업 진입에 실패하였다는 점을 주목해 볼 필요가 있다. 지역 제조업 대기업의 이탈 또는 쇠퇴는 지역 생산성 하락에 영향을 미 쳤을 가능성이 높고, 낮아진 지역 산업 생산성이 지역 경제성장률 둔화에도 영향을 미칠 가능성 이 있다. 따라서 지역의 경제활력 저하 문제를 제고하기 위해서는 지역 산업구조 개편 문제와 생 산성 향상 방안에 대한 고민이 필요하다.

개에서 발생한 양파 섭취에 의한 하인즈 소체 용혈성 빈혈 증례

이미선,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 10 month-old Yorkshire Terrier was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of lethargy, bile pigment in urine and anorexia. Blood smear showed Heinz body, polychromatocytosis, schistocytosis, anisocytosis, which were the results from the oxidative injury. Hematology demonstrated a moderate anemia(PCV 22.4%, reference rage 35-55%), which was regeneration based on the degree of RPI (4.03, reference rage > 1). Serum biochemistry demonstrated mild elevation in total bilrubin, and mild decrease in total protein and albumin concentration. A diagnosis of Heinz body hemolytic anemia secondary to onion ingestion was made and treated with fluid therapy, oxygen and antioxidant drugs. The dog recovered without any complication.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

주요 국산수종의 휨가공성 평가

정인석,이원희,장준복,배현미 한국목재공학회 2002 목재공학 Vol.30 No.2

본 연구는 고주파 가열에 의한 산벚나무, 소태나무, 서어나무, 굴참나무, 박달나무, 고로쇠나무, 피나무, 소나무, 리기다 소나무, 참오동나무의 휨가공성을 평가해 보기 위하여 실시하였다. 본 연구에서 휨가공을 위한 곡율반경은 휨틀의 곡율반경에 의해 4 ㎝, 6 ㎝, 10 ㎝으로 구분하고, 대철의 두께는 0.6 ㎜과 0.8 ㎜로 구분하였다. 본 연구결과, 소태나무, 서어나무, 박달나무 고로쇠나무는 모든 곡율반경에서 100% 성공율을 나타내었다. 산벚나무와 굴참나무의 경우 58%와 83%의 성공율을 각각 나타내었다. 피나무와 오동나무의 경우 총 29%와 8%로 매우 불량하게 나타났고, 곡율반경 4 ㎝에서는 모두 실패로 나타났다. 침엽수재인 소나무와 리기다 소나무는 각각 44%와 56%의 성공율을 나타내었다. 대철의 두께는 휨가공성의 쉽고 어려움보다는, 건조시 건조속도에 더 많은 영항을 주는 것으로 나타났다. 건조과정에서 대철두께가 얇을수록 휨가공부재의 건조 속도가 빠른 경향을 나타내었다. This study was carried out to evaluate bending property on principal domestic species such as sargent cherry(Prunus sargentii), bitter wood(Picrasma quassioides), horn beam(Carpinus laxflora), cork oak(Quercus variabilis), birch(Betula schmidtii), painted maple(Acer mono), basswood(Tilia amurensis), red pine(Pinus densiflora), pitch pine(Pinus rigida), royal pawtonia(Paulownia tomentosa) by microwave heating. In this study, radius of curvature(ROC) for bending process was classified by radius of curvature(ROC) of bending plate such as 4 ㎝, 6 ㎝, and 10 ㎝, and thickness of metal-strap(TMS) was 0.6 ㎜ and 0.8 ㎜. Bending process was successfully operated for 100 percent in bitter wood, horn beam, birch and painted maple. On the other hand, there was a success rate of 58 percent in sargent cherry and 83 percent in cork oak and 29 percent in hasswood and 8 percent in royal pawlonia which is the worst bending property. All specimens of basswood and royal pawlonia were broken at 4 ㎝ of ROC. Success rate of bending property was shown 44 percent in red pine and 56 percent in pitch pine. TMS has an effect on only drying speed in drying process than difficulty and facility of bending property. It was considered that the thinner TMS in drying process is the faster in drying speed of bent wood.

생태학으로 본 황석우 시의 자연 : 몸의 사유방식을 중심으로

전미정 우리말글학회 2004 우리말 글 Vol.32 No.-

One of the crucial subjects is nature It has been processed from the poetry, 『The song for nature』 which is representative to the poetry world of Seok-wu Hwang to the latter period This thesis deals with the poetic principle which is needed for ecology to be realized as utopia through the poem of Seok -wu Hwang He tries to prove that his primary interest in Anarchism is related to ecological poetry world Ecology and Anarchism are well associated from the beginning, finding harmony of all dichotomized relation That's why nature dealt in ecology is focused on the body Awareness of the body projected in nature implies the destruction of the dominant and subordinate relationship between human beings and nature Nature forms man and one body, that is, universe through the body Nature and man become one community like body and mind are the one Accordingly, nature filtered through Ecology is not inferior to man and mind any more. Man and mind can not exist without nature and body. Therefore the body has the most important meaning in ecological nature in this way, nature in poem of Seok-wu, Hwang is based on the principle of body. His principle of body is organism which binds nature and human being and evokes the function of community strongly And the poet tries to realize the Utopia which is harmonized world symbolically with the principle of body.

학술 커뮤니케이션의 변화와 PMC(PubMed Central)

김상준,김정아,이미나 충남대학교 사회과학대학 문헌정보학과 2002 文獻情報學論集 Vol.8 No.-