복발효 감식초의 품질

김미경,김미정,김소연,정대성,정용진,김순동 대구효성가톨릭대학교 식품과학연구소 1994 식품과학지 Vol.6 No.-

고품질의 감식초를 농가에서 자가생산하기 위한 기초적 자료를 마련할 목적으로 자연발효시킨 경우와 복발효시킨 경우의 품질을 비교하였다. 그 결과 복발효시킨 식초는 자연발효 식초에 비하여 초산농도가 1/2인 3%정도였다. 그리고 HPLC 법으로 측정한 유리당의 총함량은 자연발효 식초는 6%, 복발효시킨 것은 5%였다. 감식초내에 함유된 유기산류로는 oxalic acid, malic acid, lactic acid, citric acid, succinic acid, glutaric acid 등 이었으며 자연발효시킨 경우가 전반적으로 함량이 높았다. 특히 자연 발효시킨 경우 lactic acid함량이 높았다. 그러나 감과실의 flavour와 과실의 고유색상과 맛은 복발효시킨 경우가 강하게 나타났다. To prepare the basic information for the fermentation of high quality persimmon vinegar in the farm, the quality of the complex fermented persimmon vinegar(CFPV) was compared to that of naturally fermented persimmon vinegar(NFPV). The titratable acidity of the CFPV was 3% which was the half concentration of the NFPV. The content of total free sugar by HPLC was 6% in CFPV and NFPV was 5%. Organic acid compositions in persimmon vinegar were oxalic acid, malic acid, lactic acid, citric acid, succinic acid and glutaric acid in addition to acetic acid. Total content of the organic acids in NFPV was higer than that in CFPV. Especially, the content of latic acid in NFPV was very higher than that of CFPV. The flavour, color and taste of persimmon fruit itself in CFPV were stronger than those in NFPV.

정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.

향신료의 젖산균에 대한 항균력 비교 연구

정현진,김미경,김미정,김순동 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-

32종의 향신료를 물, 에탄올, 에틸에테르 및 에틸아세테이트로 추출하여 김치의 주발효균인 L. plantarum과 Leu. mesenteroides에 대한 항균력을 조사하였다. L. plantarum에 대한 항균력은 물 추출물에서는 caraway, cardamon, paprika, tarragon을 제외한 28종에서 항균력이 있었으며, 에틸에테르추출물에서는 arrowroot, paprika 및 tarragon을 제외한 29종이 항균력이 있었다. 에틸아세테이트추출물에서는 paprika 및 tarragon을 제외한 30종이 항균력이 있었으며, 에탄올추출물에서는 clove, paprika와 tarragon을 제외한 29종이 항균력을 나타내었다. Leu. mesenteroides에 대한 항균력은 물추출물에서는 caraway, cardamon, paprika 및 thyme을 제외한 28종에서 항균력을 나타내었으며, 에틸에테르추출물에서는 cardamon, cumin, paprika, tarragon 및 thyme을 제외한 27종이 항균력을 나타내었다. 에틸아세테이트추출물에서는 paprika 및 thyme을 제외한 30종이 항균력이 있었으며, 에탄올추출물에서는 cardamon, cumin, paprika 및 thyme을 제외한 28종이 항균력이 있었으며, 그 중 arrowroot, basil, garlic과 turmeric이 비교적 우수하였다. Leu. mesenteroides에는 항균력이 없으나 L. plantarum에는 높은 항균력을 가지는 것으로는 thyme 물추출물, cardamon, cumin 및 thyme의 에탄올추출물과 에틸에테르추출물, thyme의 에틸아세테이트추출물 등이었다. L. plantarum에 대하여 항균력을 나타내는 thyme 물추출물과 Leu. mesenteroides에 대하여 항균력을 나타내는 tarragon 물추출물을 첨가한 김치의 보존성을 조사한 결과 thyme 물추출물을 첨가한 경우에서 보존성과 품질면에서 양호하였다. 그 중 L. plantarum에 항균력이 있는 thyme 물추출물 첨가한 김치가 보존성 뿐만 아니라 맛에 있어서도 우수한 효과를 나타내었다. Antimirobial activities of 32 kinds of spices extracts on microorganisms related to kimchi fermentation were investigated. 32 kinds of spices such as allspice, arrowroot, basil, bay leaves, caraway, cardamon, celery, chilli powder, chives, cinnamon, clover, coriander, cumin, dill, fennel, garlic, horseradish, marjorarm, mugwort, mustard, nutmeg, onion, oregano, paprika, parsely, pepper, peppermint, rosemary, sage, tarragon, thyme and turmeric were extracted with water and serveral organic solvents such as ethylether, ethylacetate and ethylalcohol. Fractions were tested for antimicrobial activities against L. plantarum and Leu. mesenteroides. The extract of 28 kinds of spices except caraway, cardamon, paprika and tarragon by water extraction, those of 29 kinds except arrowroot, paprika and tarragon by ethylether extraction, those of 30 kinds except paprika and tarragon by ethylacetate extraction, and those of 29 kinds except clover, paprika and tarragon by ethylalcolhol extraction showed stong antimicrobial activities against L. plantarum. On the other hand, the extracts of 28 kinds of spices except caraway, cardamon, paprika and thyme by water extraction and those of 27 kinds except cardamon, cumin, paprika, tarragon and thyme by ethylether extraction, and those of 30 kinds except paprika and thyme by ethylacetate extraction, and those of 28 kinds except cardamon, cumin, paprika and thyme by ethylalcohol extraction showed strong antimicrobial activities against Leu. mesenteroides. Thyme extract by water extraction especially showed strong antimicrobial activities against kimchi microorganisms, finally prolonging shelf-life and enhancing quality of kimchi. The results suggest the possible use of the spices extract as natural preservatives for kimchi.

부모권력구조에 대한 아동기 경험이 여대생의 성역할 정체감에 미치는 영향 : The Influence of Childhood Experience of Parental Power Structure

김광응,유미숙,조유진 숙명여자대학교 아시아여성연구소 2004 아시아여성연구 Vol.43 No.1

본 연구는 여대생을 대상으로 부모권력구조에 대한 아동기 경험이 성역할 정체감에 어떤 영향을 미치는지를 알아보고자 하였다. 이를 위해 여대생 425명을 대상으로 부모권력구조에 대한 아동기 경험과 성역할 정체감을 측정한 자료를 분석하였다. 본 연구의 결과를 요약하면 다음과 같다. 첫째, 부모권력구조 각 하위요인(가족행사 · 친족관련 권력,자녀교육 권력, 가정경제 권력)에 대해 부우위형과 모우위형의 부모권력을 경험한 여대생은 성역할 정체감 분포에서도 다른 양상을 보였다. 반면 부모권력구조 척도의 전체점수를 합산하여 부우위형과 모우위형으로 부모권력을 구분했을 때 여대생의 성역할 정체감 분포에는 차이를 보이지 않았다. 이는 기존 부모권력 측정도구의 문제점을 지적한 연구자의 비판에 타당성을 더해주는 결과이다. 둘째, 여대생의 성역할 정체감에 영향을 미치는 변인으로 부모권력구조 하위요인 모두를 동시에 고려한 다반응 로짓분석 결과 부모권력구조 하위요인 중 자녀교육 권력과 가정경제 권력이 유의한 변인으로 나타났다. 즉, 자녀교육 권력에서 아버지가 적극적으로 참여하고 의사결정 정도가 높을 때 여대생들의 양성성과 남성성 성역할 정체감 발달 확률이 높은 반면, 가정경제 권력에서는 어머니의 참여와 의사결정 정도가 높을 때 여대생들이 남성성 성역할 정체감을 발달시킬 확률이 높은 것으로 나타났다. 한편, 가족행사 · 친족 관련 권력은 다른 하위요인의 영향을 제거했을 때 여대생의 성역할 정체감과는 관계가 없는 것으로 나타났다. The purpose of this study was to investigate how sex-role identities of female college students were related to their childhood experiences of parental power structure. Questionnaire survey was conducted with 425 female college students regarding their childhood experiences of parental power structure and their sex-role identities. Parental power structure consisted of three subfactors: parental power on family events/relative-related, child education, and home economics. It was found that the female college students formed different sex-role identity dimensions when their experiences of parental power hierarchy were measured individually in subfactors. However, they did not show differences when their experiences of parental power hierarchy were measured as the sum of subfactor scores. This supports the authors' conviction that previous measures are problematic in identifying relationships of parental power structure and children's sex-role identity. In particular, multi-response logit analysis was used in order to take into account of all the subfactors of parental power structure at the same time. As a result, childhood experiences of parental power hierarchy on child education, and on home economy were found to be closely related to the sex-role identities in the female college students. That is, the female college students, whose fathers had power on their children's education through active participation and decision-making, were likely to develop their androgyny and masculinity. On the other hand, those whose mothers had power in home economy were likely to develop masculinity. Furthermore, family events and relative-related parental power were not found to be related to the sex-role identity in female college students when the impacts of other subfactors were eliminated. It is posited that equal or balanced exertion of parental power is critical in the development of adptive and flexible sex-role identity in their children.

가토안의 Acid Phosphatase의 활성 및 분포

김태조,손해숙,송미영,이양진 曉星女子大學校 藥學大學 學生會 1990 曉星藥誌 Vol.5 No.-

Lysosomal enzyme activity of choroid, newrosensory retina, iris and ciliarybody, cornea and sclea of the rabbit eye was measured by acid phosphatase(ASPase)as enzyme maker. We reported lysosomal enzyme activity on retinal pigment epitherium in 1998. Distribution of ACPase in the tissues was carried out using Fukuzawa method and Kind-king method. Of the tissues of the rabbit eye the ACPase activity was highest in the choroid(28.6583±12.6920)by Kind-king method. The meurosensory retina(4.5433±2.2091), Iris & ciliary body(2.4138±0.8307), cornea(1.7223±0.6492)and sclea(0.9550±0.0646)also showed the specipic activity of enzyme.

데침시간에 따른 톳의 주요성분 및 항산화 활성의 변화

김진아,이종미 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.2

This study was performed to investigate the change of chemical components and antioxidant activities in Hizikia fusiformis(Harvey) OKAMURA with blanching times. For chemical composition, the contents of minerals (K, Ca, Na, Mg, Fe, Cu, Mn and Zn), vitamins(vitamin C, β-carotene and α-tocopherol) and total polyphenol were analyzed. The antioxidant activity was determined through free radicals (DPPH radical, superoxide anion radical, hydroxyl radical and hydrogen peroxide) scavenging activity and linoleic acid peroxidation inhibitory activity. As the blanching time increased, the contents of all minerals, vitamin C and total polyphenol decreased. However β-carotene and α-tocopherol were not affected by the blanching time. In addition, antioxidant activities decreased with increasing blanching time.

중합효소연쇄반응으로 조사한 관절염 환자의 관절액에서 Tropheryma whipplei DNA 검출

김성연,정문현,최새로운,김은실,이진수,박원,김미경,강재승 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : Whipple병은 Tropheryma whipplei에 의한 전신적인 감염 질환으로 소장과 관절 침범이 특징이며, 흔히 병의 초기에는 수년 동안 다른 증상 없이 관절염 소견만 보이므로, 일부 환자는 염증성 관절염으로 진단받을 가능성이 있다. 또한 류마티스 관절염을 포함하여 여러 염증성 관절염에서는 아직 병인이 밝혀지지 않았지만 감염이 관련될 가능성은 꾸준히 언급이 되었다. 저자들은 국내에서도 T. whipplei가 존재할 것이라는 가정 하에 한국인에서 중합효소연쇄반응으로 T. whipplei DNA를 조사하였다. 재료 및 방법 : 대상 환자군은 1997년부터 2002년 사이에 인하대병원 류마티스 내과에 관절 종창으로 내원한 환자들로, 류마티스 관절염 환자 33명, 골관절염 환자 18명, 기타 질환에서 관절 이상인 환자들로 5명이 포함되었다. 건강한 성인 8명의 타액과 22명의 위내시경을 받은 환자의 위액을 채취하여 검사하였다. 중합효소연쇄반응은 T. whipplei heat shock protein의 일부 유전자를 증폭할 수 있는 2개의 시동체를 사용하였다. 표준 균주를 얻기가 어려워 양성 대조군 없이 중합효소연쇄반응을 시행하였다. 결과 : 관절염 환자의 관절액, 정상인의 타액, 위내시경을 받은 사람의 위액에서 모두 중합효소 연쇄반응으로 핵산을 증명할 수 없었다. 결론 : 양성대조군이 없어 검사 잘못일 가능성도 배제할 수 없으나, Whipple병이 염증성 관절염의 흔한 원인이 아니거나 관절의 직접 침범보다는 위장관 감염 후 생기는 면역 반응에 의한 반응성 관절염일 가능성이 있다. 관절액 이외의 검체에서 조사한다면 다른 결과를 얻을 수도 있으리라 생각하며 앞으로 이들에 대한 조사가 필요하다. Background : Whipple's disease is a chronic systemic infection caused by Tropheryma whipplei which chiefly involves gastrointestinal tract and joints. Because arthritis is often the first sign of Whipple's disease, Whipple's disease may be misdiagnosed in the early stage of illness as non-infectious inflammatory arthritis. Rheumatoid arthritis may also be caused by infectious agents, including T. whipplei. To elucidate the relationship between T. whipplei infection and various arthritis, we examined T. whipplei DNA in Korean patients by polymerase chain reaction (PCR). Materials and Methods : With two T. whipplei-specific primers dervied from the partial heat shock protein 65 gene sequence, PCR was performed on 56 joint fluid samples : 33 samples from RA patients, 18 samples from OA patients, and 5 samples from patients with other unflammatory arthritis. In addition, the same method was applied to find out the DNA of T. whipplei in 8 normal adults' saliva and 22 patients' gastric juice. Due to the difficulty in obtaining the reference strain of T. whipplei, the PCR was performed without the positive control. Results : The PCR method did not reveal T. whipplei in joint fluid from 56 patients with arthropathies, 8 samples from saliva, and 22 samples from gastric juice. Conclusion : Though technical errors can not be excluded as a cause of absence of a positive case, our results suggest that T. whipplei infection may not be a common cause of various arthritis in Korea; T. whipplei may be associated with inflammatory arthritis indirectly by immune-mediated mechanism rather than by direct joint invasion. The examination of specimens other than joint fluid could be helpful and the study of larger number of patient is highly desirable.

데빅병 치료에 사용한 Interferon β-1b의 부작용 1예

김정미,조은경,조정선,최용석,한영수,한정호,김두응,최진영 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

A 63 year-old woman who had diagnosis of multiple sclerosis complaining left hemiparesis and fluctuating decreased visual acuity in her right eye and abnormal findings in pattern shift visual evoked potentials(PSVEPs) and on T2 weighted MR scanning. We treated her subcutaneously with 250μg interferon (3-lb(ITFß -lb) every other day. There were detected pancytopenia, skin rash, and abnormal liver function test seven days after the therapy. In spite of reducing the dose of interferon injection, abnormal laboratory findings were not returned to normal. Dose adjustment of interferon-I3 for body weight or race, may be helpful to reduce the side effects.

사진을 찍읍시다

김진미,임숙영,정진희 이화여자대학교 사범대학 시청각교육과 1983 시청각 교육 Vol.- No.13

폐결핵치료 중인 환자에서 Rifampicin에 의해 유발된 위막성 대장염 2예

김봉진,권균홍,임창섭,김자영,홍정범,옥미선,배용목,김지연 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

Pseudomembranous colitis (PMC) is a disease caused by Clostridium difficile proliferation. The causative drugs are clindamycin, third-generation cephalosporins, flouroquinolone and so on. Rifampicin has been reported as a cause of PMC in the 1980s, and the frequency of PMC is increasing because rifampicin is a first line drug for anti-tuberculosis therapy. Two patients were recently admitted to our hospital due to watery diarrhea for 1 month and they were diagnosed with PMC by sigmoidoscopy. Their onset age were 74, 72 years old and latent period of symptoms were 60, 129 days, respectively. In one case, the patient displayed coexisting hypertension and diabetes. The clinical symptoms improved after discontinuing the rifampicin and then administering oral metronidazole. We report here on two cases presumed to be rifampicin-induced PMC.