Exterior egg quality as affected by enrichment resources layout in furnished laying-hen cages

Li, Xiang,Chen, Donghua,Meng, Fanyu,Su, Yingying,Wang, Lisha,Zhang, Runxiang,Li, Jianhong,Bao, Jun Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.10

Objective: This study aimed to investigate the effects of enrichment resources (a perch, dustbath, and nest) layout in furnished laying-hen cages (FC) on exterior quality of eggs. Methods: One hundred and sixty-eight (168) Hy-Line Brown laying hens at 16 weeks of age were randomly distributed to four treatments: small furnished cages (SFC), medium furnished cages type I (MFC-I), medium furnished cages type II (MFC-II), and medium furnished cages type III (MFC-III). Each treatment had 4 replicates or cages with 6 hens for SFC (24 birds for each SFC) and 12 hen/cage for MFC-I, -II, and -III (48 birds for each MFC-I, -II and -III). Following a 2-week acclimation, data collection started at 18 weeks of age and continued till 52 weeks of age. Dirtiness of egg surface or cracked shell as indicators of the exterior egg quality were recorded each week. Results: The results showed that the proportion of cracked or dirty eggs was significantly affected by the FC type (p<0.01) in that the highest proportion of cracked or dirty eggs was found in MFC-I and the lowest proportion of dirty eggs in SFC. The results of this showed that furnished cage types affected both dirty eggs and cracked eggs (p<0.01). The results also indicated that not nest but dustbath lead to more dirty eggs. Only MFC-I had higher dirty eggs at nest than other FC (p<0.01). The results of dirty eggs in MFC-I and MFC-II compared with SFC and MFC-III seemed suggest that a low position of dustbath led to more dirty eggs. Conclusion: SFC design affected exterior egg quality and the low position of dustbath in FC resulted in higher proportion of dirty eggs.

Zero-watermarking Algorithm for Medical Volume Data Based on Legendre Chaotic Neural Network and Perceptual Hashing

Baoru Han,Lisha Cai,Wenfeng Li 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.1

Medical information digitization makes the medical information storage and extraction more convenient. Medical image information security and copyright protection is also gradually being taken seriously, and some medical image watermarking has been applied. According to the characteristics of three-dimensional medical images, this paper proposes a robust zero-watermarking algorithm for medical volume data based on legendre chaotic neural network and perceptual hashing. The algorithm is based on three-dimensional discrete wavelet transform frequency analysis features, which uses perceptual hashing technique to extract medical volume data itself feature vector in order to structure robust zero watermarking. And using legendre chaotic neural network to generate chaotic sequence to enhance the security of the watermarking. The algorithm achieves a combination of legendre chaotic neural network encryption and zero-watermarking technology, which can improve the medical volume data watermarking algorithm security and robustness. The simulation results show the effectiveness of the algorithm.

Illumina-sequencing based transcriptome study of coat color phenotypes in domestic goats

Yongdong Peng,Xiaohui Liu,Liying Geng,Ruxue Ma,Lisha Li,Jingshi Li,Chuansheng Zhang,Zhengzhu Liu,Yuanfang Gong,Xianglong Li 한국유전학회 2017 Genes & Genomics Vol.39 No.8

This study performed a comprehensive expression profiling of genes expressed in the skin of goats with three different coat colors by Illumina Sequencing. A total of 91 significantly expressed genes were detected when comparing gray skin to white skin library and these included 74 up-regulated and 17 down-regulated genes in gray skin. There were 67 differentially expressed genes between brown skin and white skin libraries, 23 of which were up-regulated and 44 were down-regulated in brown skin. When we compared brown and gray libraries, 154 differentially expressed genes were found, of which 33 showed higher expression and 121 showed lower expression in brown skin. To our surprise, MC1R, MITF, TYR and KIT showed no significant difference in expression between the goats with three skin colors, whereas ASIP was detected in white skin but not in dark skins. In this study, PMEL, TRPM1, TYRP1 and DCT were significantly upregulated in brown goat skin compare with gray and white skins. PMEL showed higher expression in gray goat skin compared with white goat skin, whereas there were no significant differences in the expression of TYRP1, TRPM1 and DCT between gray and white skin samples. In addition, ELOVL3 showed higher expression in gray goat skin than in brown and white skins, whereas there was no significant differences in the expression of ELOVL3 between brown and white skin samples. These results expand our understanding of the complex molecular mechanisms of skin physiology and melanogenesis in goat and provide a foundation for future studies.

Numerical study on the cavitating flow through poppet valves concerning the influence of flow instability on cavitation dynamics

Cong Yuan,Lisha Zhu,Shiqi Liu,Du Zunling,He Li 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.2

The flow dynamics of the cavitating jet through poppet valves inevitably suffers from the influence of varying openness, but the relevant mechanism remains unclear. Hence, the present study conducts a numerical study on the cavitating flow phenomenon inside poppet valves with two valve seat structures, in order to examine the flow mechanisms underlying varying cavitation phenomenon at different openness. According to the results, the overall cavitation distribution as well as the morphology feature follows a similar pattern due to the same inception mechanism irrelevant of the openness amount. However, examination of the dynamic behaviors confirms discernable variation in 2 coupling effects between flow instability and cavitation. The flow separation at the poppet trailing edge produces non-cavitation flow instability at 0.4 mm openness and attached cavitation at 0.8 mm, which amalgamate with upstream-shed vortex cavitation. The stably attached cavitation within the chamfered groove at 0.4 mm, as the source of non-cavitation flow instability, and the detached cavitation at 0.6 and 0.8 mm openness produce vortex cavitation at the free shearing side. The substantial variation in these two coupling effects contributes sensibly to the increase in the overall cavitation intensity. Meanwhile, the weakened coupling effect between the free shearing vortex and wall shearing vortex, as a consequence of increased potential core thickness at larger openness, leads to the attenuated vortex cavitation at the wall poppet side. Additionally, the vortex cavitation at free shearing side has a larger size in the chamfered valve seat case, due to the coupling of shed flow instability with the Kelvin-Helmholtz instability. As a conclusion, the inception mechanism for the cavitation remains unchanged with openness, while the difference in flow instabilities as well as the associated coupling effects with cavitation contributes to the variation in cavitation intensity.

A Single-Arm Phase II Study of Nab-Paclitaxel Plus Gemcitabine and Cisplatin for Locally Advanced or Metastatic Biliary Tract Cancer

Ting Liu,Qing Li,Zhen Lin,Chunhua Liu,Wei Pu,Shasha Zeng,Jun Lai,Xuebin Cai,Lisha Zhang,Shuyang Wang,Miao Chen,Wei Cao,Hongfeng Gou,Qing Zhu 대한암학회 2024 Cancer Research and Treatment Vol.56 No.2

Purpose Patients with advanced biliary tract cancer (BTC) have a poor survival. We aim to evaluate the efficacy and safety of nab-paclitaxel plus gemcitabine and cisplatin regimen in Chinese advanced BTC patients.Materials and Methods Eligible patients with locally advanced or metastatic BTC administrated intravenous 100 mg/m² nab-paclitaxel, 800 mg/m² gemcitabine, and 25 mg/m² cisplatin every 3 weeks. The primary endpoint was progression-free survival (PFS). The secondary endpoints included overall survival (OS) and adverse events, while exploratory endpoint was the association of biomarkers with efficacy.Results After the median follow-up of 25.0 months, the median PFS and OS of 34 enrolled patients were 7.1 months (95% confidence interval [CI], 5.4 to 13.7) and 16.4 months (95% CI, 10.9 to 23.6), respectively. The most common treatment-related adverse events at ≥ 3 grade were neutropenia (26.5%) and leukopenia (26.5%). Survival analyses demonstrated that carcinoembryonic antigen (CEA) levels could monitor patients’ survival outcomes. A significant increase in the number of infiltrating CD4+ cells (p=0.008) and a decrease in programmed death-1–positive (PD-1+) cells (p=0.032) were observed in the response patients.Conclusion In advanced BTC patients, nab-paclitaxel plus gemcitabine and cisplatin regimen showed therapeutic potential. Potential prognostic factors of CEA levels, number of CD4+ cells and PD-1+ cells may help us maximize the efficacy benefit.

Temperature dependent defence of Nicotiana tabacum against Cucumber mosaic virus and recovery occurs with the formation of dark green islands

Feifei Zhao,Yanan Li,Li-juan Chen,Lisha Zhu,Han Ren,Honghui Lin,Dehui Xi 한국식물학회 2016 Journal of Plant Biology Vol.59 No.3

Temperature is an important environmental factor controlling plant growth, development, and immune response. However, the role of temperature in plant disease resistance is still elusive. In the present study, the potential effects of temperature on the interaction between Nicotiana tabacum and Cucumber mosaic virus (CMV) were investigated. Our results indicated that N. tabacum plants displayed severe symptoms at early stage of post inoculation at high temperature (HT, 28°C), associated with higher viral replication level, more serious stress damages. By contrast, low temperature (LT, 18°C) effectively delayed the replication of CMV compared with elevated temperatures. Additionally, quantitative real-time PCR analyses revealed that lower temperatures (≤ 24°C) promote salicylic acid (SA) dependent responses, whereas higher temperatures (> 24°C) activate the genes expression of jasmonic acid (JA) pathway. Interestingly, the dark green islands (DGIs) appeared much earlier in CMV-inoculated plants grown at HT compared with those at LT and the accumulation of virus small interfering RNAs in plants were significantly up-regulated under elevated temperatures at early stage of post inoculation. Taken together, these results indicated that temperature changes had important effects on plant defence response, and different temperatures could induce different immune pathways of N. tabacum against CMV infection.

Effect of social order, perch, and dust-bath allocation on behavior in laying hens

Wang Yanan,Zhang Runxiang,Wang Lisha,Li Jianhong,Su Yingying,Li Xiang,Bao Jun 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: The objective of this study was to determine the effects of different social ranking order (SRO) and the enrichments (perch and dust-bath) allocation (EA) on behavior of laying hens in furnished cages. Methods: Total experimental period was 4 weeks. There were 216 Hy-line brown layers beak-trimmed at 1 d of age and selected randomly at 14 weeks of age from a commercial farm, and randomly divided into 36 cages with 6 hens in each cage. High enrichments (perch and dust-bath) allocation (HEA) and low enrichments (perch and dust-bath) allocation (LEA) were provided. Video observations of behavior were obtained from the focal hens between 14 and 18 weeks of age and perching, dust-bathing and other general behaviors of the hens with different social orders were measured. Results: Perching behavior of high SRO hens (HSR) were significantly higher than that of medium SRO hens (MSR), and that of the MSR were significantly higher than that of low SRO hens (LSR) (p<0.01), except for lying on perch (p>0.05). The hens in the high EA cage (HEAC) showed more lying behavior on perch than those in the low EA cage (LEAC) (p< 0.01). The different SRO and EA did not affect dust-bathing behavior except vertical wingshaking behavior (p<0.05). The LEA did not affect general behaviors (p>0.05), except standing and preening behaviors (p<0.01 and p<0.05), of which the hens in the HEAC showed less standing (p<0.01) and more preening behavior than the hens in the LEAC. Conclusion: The SRO of laying hens has a significant effect on the perching behaviors, but SRO and EA have little effect on dust-bathing and general behaviors. Objective: The objective of this study was to determine the effects of different social ranking order (SRO) and the enrichments (perch and dust-bath) allocation (EA) on behavior of laying hens in furnished cages.Methods: Total experimental period was 4 weeks. There were 216 Hy-line brown layers beak-trimmed at 1 d of age and selected randomly at 14 weeks of age from a commercial farm, and randomly divided into 36 cages with 6 hens in each cage. High enrichments (perch and dust-bath) allocation (HEA) and low enrichments (perch and dust-bath) allocation (LEA) were provided. Video observations of behavior were obtained from the focal hens between 14 and 18 weeks of age and perching, dust-bathing and other general behaviors of the hens with different social orders were measured.Results: Perching behavior of high SRO hens (HSR) were significantly higher than that of medium SRO hens (MSR), and that of the MSR were significantly higher than that of low SRO hens (LSR) (p<0.01), except for lying on perch (p>0.05). The hens in the high EA cage (HEAC) showed more lying behavior on perch than those in the low EA cage (LEAC) (p<0.01). The different SRO and EA did not affect dust-bathing behavior except vertical wing-shaking behavior (p<0.05). The LEA did not affect general behaviors (p>0.05), except standing and preening behaviors (p<0.01 and p<0.05), of which the hens in the HEAC showed less standing (p<0.01) and more preening behavior than the hens in the LEAC.Conclusion: The SRO of laying hens has a significant effect on the perching behaviors, but SRO and EA have little effect on dust-bathing and general behaviors.

Development of Toxoplasma gondii Chinese I genotype Wh6 Strain in Cat Intestinal Epithelial Cells

Gui-Hua Zhao,Lixin Zhang,Lisha Dai,Haozhi Xu,Chao Xu,Ting Xiao,Jin Li,Hui Sun,Beibei Zhou,Kun Yin 대한기생충학ㆍ열대의학회 2022 The Korean Journal of Parasitology Vol.60 No.4

Felids are the unique definitive host of Toxoplasma gondii. The intestine of felid is the only site for initiating Toxoplasma gondii sexual reproduction. T. gondii excretes millions of infectious oocysts from the intestine, which are the primary source of infection. There are many difficulties in developing vaccines and drugs to control oocyst excretion due to the lack of an appropriate experimental model. Here, we established an in vitro feline intestinal epithelial cell (IEC) infection system and an efficient animal model of T. gondii Chinese 1 genotype, Wh6 strain (TgCtwh6). The Kunming mice brain tissues containing TgCtwh6 cysts were harvested 42-day post-infection. The bradyzoites were co-cultured with cat IECs in vitro at a ratio of 1:10. Five 3-month-old domestic cats were orally inoculated with 600 cysts each. The oocysts were detected by daily observation of cat feces by microscopy and polymerase chain reaction. We found that the parasite adhered and invaded cat IECs in vitro, transformed into tachyzoites, and then divided to form rose-like structures. These parasites eventually destroyed host cells, escaped, and finished the asexual reproduction process. Schizonts associated with sexual reproduction have not been observed during development in vitro cultured cells. However, schizonts were detected in all infected cat intestinal epithelial cells, and oocysts were presented in all cat feces. Our study provides a feasible cell model and an efficient infection system for the following studies of T. gondii sexual reproduction, and also lays a foundation to develop drugs and vaccines for blocking excretion and transmission of oocysts.

Impact of sorption functional media (SFM) from zeolite tailings on the removal of ammonia nitrogen in a biological aerated filter

Yan Feng,Yanzhen Yu,Liping Qiu,Yawen Yang,Zhigang Li,Miaowan Li,Lisha Fan,Yuzhong Guo 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1

Haydite and sorption functional media (SFM),made of clinoptilolite tailings, were used as filter media forbiological aerated filter (BAF) to treat synthetic wastewater in parallel. The aim of this work was toevaluate impact of SFM on the removal of ammonia nitrogen in a biological aerated filter reactor underammonium nitrogen shock loads. It could be demonstrated that: (1) the BAF with SFMpresented higherammonia nitrogen removal efficiency than that with haydite; (2) during the phase of higher influentconcentrations SFMs were collecting ammonia nitrogen and can buffer ammonia nitrogen loads anddampen the effluent concentration of ammonia nitrogen; (3) when the influent concentration decreasesammonia nitrogen will desorb from the SFM and can be utilized by the nitrifying bacteria growing on theSFM. Therefore, with the SFM application existing BAF unites can be upgraded and the stability of theoperation process of BAF can be improved significantly.

Statistical Analysis of Dry Sliding Wear Process Parameters for AZ91 Alloy Processed by RD-ECAP Using Response Surface Methodology

Bassiouny Saleh,Jinghua Jiang,Qiong Xu,Reham Fathi,Aibin Ma,Yuhua Li,Lisha Wang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.8

The main objective of this paper is to study the influence of wear testing parameters on the weight loss of AZ91 magnesiumalloy which was developed via rotary die-equal channel angular pressing (RD-ECAP) method through the different numberof passes. The AZ91 sample was pressed up to 16 passes in order to decrease the grain size to 10 μm. After RD-ECAP processing,significant grain refinement was found, which yielded a refined grain size after 16 passes and enhanced mechanicalproperties and wear resistance. Dry sliding wear experiments with a ball on disc apparatus at room temperature were conducted,following RD-ECAP processing. The response surface methodology (RSM) was used to optimize and compare thewear resistance of the RD-ECAP alloy and the as-received alloy. Analysis of the wear mechanism and the worn surfaces ofAZ91 samples was performed using scanning electron microscope. RSM analysis showed that increased applied load, slidingspeed and sliding time in all cases decreased wear resistance. On the other side, wear resistance in AZ91 magnesium alloywas improved by the increase in the number of passes of RD-ECAP. The three empirical models were validated by conductingconfirmation tests and certified that the proven empirical wear models are reliable for wear applications as a predictive tool.