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RISS 인기검색어
- 검색키워드
- 저자 : Yongdong Peng (검색결과 3 건)
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Illumina-sequencing based transcriptome study of coat color phenotypes in domestic goats
Yongdong Peng,Xiaohui Liu,Liying Geng,Ruxue Ma,Lisha Li,Jingshi Li,Chuansheng Zhang,Zhengzhu Liu,Yuanfang Gong,Xianglong Li 한국유전학회 2017 Genes & Genomics Vol.39 No.8
This study performed a comprehensive expression profiling of genes expressed in the skin of goats with three different coat colors by Illumina Sequencing. A total of 91 significantly expressed genes were detected when comparing gray skin to white skin library and these included 74 up-regulated and 17 down-regulated genes in gray skin. There were 67 differentially expressed genes between brown skin and white skin libraries, 23 of which were up-regulated and 44 were down-regulated in brown skin. When we compared brown and gray libraries, 154 differentially expressed genes were found, of which 33 showed higher expression and 121 showed lower expression in brown skin. To our surprise, MC1R, MITF, TYR and KIT showed no significant difference in expression between the goats with three skin colors, whereas ASIP was detected in white skin but not in dark skins. In this study, PMEL, TRPM1, TYRP1 and DCT were significantly upregulated in brown goat skin compare with gray and white skins. PMEL showed higher expression in gray goat skin compared with white goat skin, whereas there were no significant differences in the expression of TYRP1, TRPM1 and DCT between gray and white skin samples. In addition, ELOVL3 showed higher expression in gray goat skin than in brown and white skins, whereas there was no significant differences in the expression of ELOVL3 between brown and white skin samples. These results expand our understanding of the complex molecular mechanisms of skin physiology and melanogenesis in goat and provide a foundation for future studies.
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Hair follicles transcriptome profiles in Bashang long-tailed chickens with different plumage colors
Xiaohui Liu,Rongyan Zhou,Yongdong Peng,Chuansheng Zhang,Lanhui Li,Chunxiang Lu,Xianglong Li 한국유전학회 2019 Genes & Genomics Vol.41 No.11
Despite the rich variety in plumage color found in nature, genetic studies on how hair follicles affect pigmentation are often limited to animals that have black and white pigment. To test how gene expression influences plumage color, transcriptomes of chicken hair follicles with white, black, hemp, reed catkins, silvery grey, and landscape plumage colors were generated using Illumina sequencing. We generated six RNA-Seq libraries with over 25 million paired-end clean reads per library with percentage of paired-end clean reads ranging from 96.73 to 96.98%. 78% of the reads mapped to the chicken genome, and approximately 70% of the reads were mapped to exons and 6% mapped to introns. Transcriptomes of hair follicles producing hemp and land plumage were similar, but these two showed moderate differences compared with gray and reed colored plumage. The black and white follicle transcriptomes were most divergent from the other colors. We identified several candidate genes, including GPNMB, PMEL, TYRP1, GPR143, OCA2, SOX10, SLC45A2, KRT75, and TYR . All of these genes are known to induce pigment formation in mice. White feathers result from the lack of pigment formation, and our results suggest that the white chickens due to the recessive insertion mutation of TYR . The formation of black area size and color depth may be due to the expression levels of GPNMB, PMEL, TYRP1, GPR143, OCA2, SOX10, SLC45A2, KRT75, and TYR . The GO analysis of the differentially expressed genes (DEGs) revealed that DEGs in our transcriptome analysis were enriched in cytoskeleton and cell structure related pathways. The black plumage transcriptome showed significant differences in melanogenesis, tyrosine metabolism, and riboflavin metabolism compared with transcriptomes of other plumage colors. The transcriptome profiles of the different chicken plumage colors provide a valuable resource to understand how gene expression influences plumage color, and will be an important resource for identifying candidate genes in breeding programs.
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Ultimate Lateral Bearing Capacity and Group Effect of Belled Wedge Pile Groups
Gangqiang Kong,Huaifeng Peng,Hongyu Qin,Lehua Wang,Yongdong Meng 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.12
Pile foundation of ports, high-voltage transmission line tower are subjected to amount of lateral loading, prediction on lateral bearing capacity is one of the most important projects in structure design. This paper pertains to the model tests on pile-soil interaction of single pile, 2 × 1 and 2 × 2 belled wedge pile groups embedded in sand under lateral load. The load versus displacement, and the soil pressures along depth surrounding piles are measured, the ultimate lateral bearing capacities and group effects of belled wedge piles are analyzed and discussed. An simplified theoretical calculation method on predicting the lateral bearing capacities of shaped pile groups with considering group pile p-y curves, and longitudinal cross-section variation are proposed. The accuracy and reliability of this developed calculation method are verified through the comparative analysis with model test results obtained in this study and previous literature. The predicted values of lateral bearing capacities have suitable agreement with the measured data. It also shows that the ultimate lateral bearing capacities of belled wedge pile group are nearly 1.8 − 2.0 times of those of traditional belled pile with the same concrete usage in this study’s condition.
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