Sialoglycoprotein from Gadous morhua eggs improve high bone turnover activity via down-regulating BMP-2/Smads and Wnt/b-catenin signal pathways

Kai Wang,Lihua Han,Na Wang,Yiming Wang,Jingfeng Wang 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.5

The effect of sialoglycoprotein isolated from Gadous morhua eggs (Gm-SGP) on ovariectomized (OVX) induced osteoporosis, which is characterized by high bone turnover activity was investigated. Results revealed that Gm-SGP significantly increased bone mineral density, enhanced bone biomechanical properties and repaired the microstructure of the trabecular bone. Also, the treatment with Gm-SGP remarkably decreased biochemical marker contents or activities, such as serum BALP, PICP, BMP-2, TrACP, Cath-K, urine Ca and P, leading to the reduction in bone turnover. The elevation in the rate of bone formative process contributed in the increase of bone turnover. Both BMP-2/Smads and Wnt/b-catenin signaling pathways played an important role in osteogenesis. Gm-SGP suppressed the key factors expression in these two pathways such as BMP-2, Smad1, Smad4, Lrp-5b, Runx2, Osx, ALP, Col1, OCN and b-catenin. These findings might provide some theoretical basis for the application of Gm-SGP as a potential anti-osteoporotic drug or as functional food.

Green-revertible Chlorina 1 (grc1) is Required for the Biosynthesis of Chlorophyll and the Early Development of Chloroplasts in Rice

Jieqin Li,Yihua Wang,Juntao Chai,Lihua Wang,Chunming Wang,Wuhua Long,Di Wang,Yunlong Wang,Ming Zheng,Cheng Peng,Mei Niu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.5

The nuclear genes involved in chloroplast developmentand chlorophyll biosynthesis must be investigated tounderstand their functions in plant growth and development. In this study, we isolated and identified a unique leaf-colormutant of rice with a green-yellow phenotype before thefour-leaf stage and named the mutation green-revertiblechlorina 1 (grc1). The mutants had significantly lower plantheight, number of tillers, and panicle length and headedsignificantly earlier than the wild type. The levels ofchlorophylls, carotenoids, and chlorophyll precursors werealso lower. The mutation in grc1 affected chloroplastultrastructure, particularly thylakoid development. Geneticanalysis indicated that the green-yellow phenotype wascontrolled by a single recessive gene. We mapped the grc1gene to a 32.4-kb region on the long arm of chromosome 6. Through map-based cloning, we identified a 45-bp insertionin the genomic region of LOC_Os06g40080, which encodeda heme oxygenase. Expression of LOC_Os06g40080 wassignificantly down-regulated in the grc1 mutant. Subcellularlocalization showed that this heme oxygenase was localizedin the chloroplast. In summary, we isolated and identified thegene for grc1, which plays an important role in chlorophyllbiosynthesis and chloroplast development in rice.

Radial basis collocation method for dynamic analysis of axially moving beams

Wang, Lihua,Chen, Jiun-Shyan,Hu, Hsin-Yun Techno-Press 2009 Interaction and multiscale mechanics Vol.2 No.4

We introduce a radial basis collocation method to solve axially moving beam problems which involve $2^{nd}$ order differentiation in time and $4^{th}$ order differentiation in space. The discrete equation is constructed based on the strong form of the governing equation. The employment of multiquadrics radial basis function allows approximation of higher order derivatives in the strong form. Unlike the other approximation functions used in the meshfree methods, such as the moving least-squares approximation, $4^{th}$ order derivative of multiquadrics radial basis function is straightforward. We also show that the standard weighted boundary collocation approach for imposition of boundary conditions in static problems yields significant errors in the transient problems. This inaccuracy in dynamic problems can be corrected by a statically condensed semi-discrete equation resulting from an exact imposition of boundary conditions. The effectiveness of this approach is examined in the numerical examples.

Local adaptation of central policies: the policymaking and implementation of compulsory education for migrant children in China

Lihua Wang 서울대학교 교육연구소 2016 Asia Pacific Education Review Vol.17 No.1

This article looks at the central and local governments’ policymaking and implementation of compulsory education for migrant children in China. Three distinct models of policy implementation were identified through a case study approach. They indicated a selective adaptation of central policy objective and principles by the local governments and revealed diverse conditions of compulsory education for migrant children across the nation. In spite of substantial improvement over the years, migrant children still could not receive equitable compulsory education as urban children, due to three system barriers in general and local policy differences in particular.

Effects of chronic alcohol exposure on ischemia–reperfusion-induced acute kidney injury in mice: the role of β-arrestin 2 and glycogen synthase kinase 3

Lihua Wang,Yifei Zhu,Lili Wang,Jingjing Hou,Yongning Gao,Lei Shen,Jingyu Zhang 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Little is known about the effects of chronic alcohol intake on the outcome of acute kidney injury (AKI). Hence, we examined the effects of chronic alcohol intake on the development of renal fibrosis following AKI in an animal model of bilateral renal ischemia–reperfusion (IR) injury. We first found that chronic alcohol exposure exacerbated bilateral IR-induced renal fibrosis and renal function impairment. This phenomenon was associated with increased bilateral IR-induced extracellular matrix deposition and an increased myofibroblast population as well as increased bilateral IR-induced expression of fibrosis-related genes in the kidneys. To explore the mechanisms underlying this phenomenon, we showed that chronic alcohol exposure enhanced β-arrestin 2 (Arrb2) expression and Akt and glycogen synthase kinase-3 (GSK3)β activation in the kidneys. Importantly, pharmacological GSK3 inhibition alleviated bilateral IR-induced renal fibrosis and renal function impairment. Furthermore, we demonstrated that Arrb2− / − mice exhibited resistance to IR-induced renal fibrosis and renal function impairment following chronic alcohol exposure, and these effects were associated with attenuated GSK3β activation in the kidneys. Taken together, our results suggest that chronic alcohol exposure may potentiate AKI via β-arrestin 2/Akt/GSK3β-mediated signaling in the kidney.

Geometric accuracy of incremental sheet forming for TRIP590

Lihua Li,Jin Wang,Baoping Wang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11

TRIP steels have both high strength and good ductility, and they are typically used in automotive industry for the purpose of safety and energy saving. Deforming TRIP steel by ISF is practically valuable, but the application is significantly restricted by its geometric accuracy. At present, there is not enough research on geometric accuracy for TRIP steels. In this study, we explored the geometric accuracy of ISF for TRIP590 by referring to 1060Al steel and 08F steel. We found that the accuracy of TRIP590 steel was the lowest, which was because of the phase transformation of the material which occurred during deformation. To verify this, X-ray diffraction was employed to figure out the volume fraction of retained austenite. The results have shown that there is always TRIP effect during ISF process, and the relationship between natural logarithm of austenite volume fraction and the equivalent strain is negative linear. Lastly, it is proved that multiple reverse compensations could improve the geometric accuracy for TRIP steels.

Nucleos(t)ide Analogues for Reducing Hepatocellular Carcinoma in Chronic Hepatitis B Patients: A Systematic Review and Meta-Analysis

( Xinhui Wang ),( Xiaoli Liu ),( Zhibo Dang ),( Lihua Yu ),( Yuyong Jiang ),( Xianbo Wang ),( Zhiyun Yang ) 대한간학회 2020 Gut and Liver Vol.14 No.2

Background/Aims: Studies have shown that nucleos(t)ide analogue (NA) treatment can reduce the risk of hepatocellular carcinoma (HCC) in chronic hepatitis B (CHB) patients, but it is unclear which NA is most effective. We performed a meta-analysis and systematic review comparing the efficacies of NAs in CHB patients. Methods: We searched literature databases for randomized controlled trials (RCTs) and observational studies that analyzed the hepatic biochemical response, virological response, seroconversion rate, drug resistance rate, and HCC incidence rate in CHB patients treated with NAs. Meta-analyses were performed with RevMan and Stata/SE software. Results: Twelve cohort studies and one RCT were selected, in which entecavir (ETV), lamivudine (LAM), telbivudine (LdT), and/or tenofovir disoproxil fumarate (TDF) were evaluated in CHB patients. The meta-analysis showed that ETV was superior to LAM with regard to the HCC incidence (p<0.001), biochemical response (p=0.001), virological response (p=0.02), and drug resistance (p<0.001), and ETV was superior to LdT with regard to the virological response (p<0.001) and drug resistance (p<0.001). We found no significant difference between ETV and TDF with regard to the HCC incidence (p=0.08), biochemical response (p=0.39), virological response (p=0.31), serological conversion (p=0.38), or drug resistance (p=0.95). NA-treated patients with pre-existing cirrhosis had a 5.49 times greater incidence of HCC than those without cirrhosis (p<0.001). Conclusions: ETV or TDF should be used for long-term first-line monotherapy in CHB patients according to the current guidelines. Standardized protocols are needed for future studies of ETV and TDF to facilitate conclusive comparisons. Patients with cirrhosis are at significantly elevated risk for HCC, despite the benefits of NA treatment. (Gut Liver 2020;14:232-247)

Genome-wide identification of auxin-responsive microRNAs in the poplar stem

Yang Lihua,Ping Tao,Lu Wenjin,Song Sangfa,Wang Jianli,Wang Qiao,Chai Guohua,Bai Yue,Chen Yan 한국유전학회 2023 Genes & Genomics Vol.45 No.8

Background Wood (secondary xylem) of forests is a material of great economic importance. Wood development is strictly controlled by both the phytohormone auxin and microRNAs (miRNAs). Currently, the regulatory mechanisms underlying wood formation by auxin-associated miRNAs remain unclear. Objective This report was designed to identify auxin-responsive miRNAs during wood formation. Methods Morphological observation of wood development in the poplar stems was performed under the treatment of different concentrations (0 mg/L, CK; 5 mg/L, Low; 10 mg/L, High) of indol-3-butyric acid (IBA). Using a small RNA sequencing strategy, the effect of IBA treatment on miRNAs expression was genome-widely analyzed. Results In this study, we found that wood development of poplar was promoted by low concentration of IBA treatment but inhibited by high concentration of IBA treatment. Stringent bioinformatic analysis led to identification of 118 known and 134 novel miRNAs candidates. Sixty-nine unique developmental-related miRNAs, corresponding to 269 target genes, exhibited specific expression patterns in response to auxin, as was consistent with the influence of auxin application on wood formation. Three novel miRNAs had the most number (≥ 9) of target genes, belonging to SPL, GRF and ARF gene families. The evolutionary relationships and tissue expression patterns of 41 SPL, GRF and ARF genes in poplar were thus analyzed. Of them, four representative members and corresponding miRNAs were confirmed using RT-qPCR. Conclusions Our results may be helpful for a better understanding of auxin-induced regulation of wood formation in tree species.

Fundamentals and Practical Design of Traction Inverter for Hybrid Electric Vehicles

Lihua Chen,Jin Wang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Electrification of vehicles is a non-reversible trend Current xEVs based applications have pushed the boundary of power electronics in terms of power density, efficiency and reliability New WBG based devices are evolving and promising for automotive applications Cost and reliability for WBGs are still the main barriers for automotive applications

Modeling and performance evaluation of a piezoelectric energy harvester with segmented electrodes

Wang, Hongyan,Tang, Lihua,Shan, Xiaobiao,Xie, Tao,Yang, Yaowen Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.14 No.2

Conventional cantilevered piezoelectric energy harvesters (PEHs) are usually fabricated with continuous electrode configuration (CEC), which suffers from the electrical cancellation at higher vibration modes. Though previous research pointed out that the segmented electrode configuration (SEC) can address this issue, a comprehensive evaluation of the PEH with SEC has yet been reported. With the consideration of delivering power to a common load, the AC outputs from all segmented electrode pairs should be rectified to DC outputs separately. In such case, theoretical formulation for power estimation becomes challenging. This paper proposes a method based on equivalent circuit model (ECM) and circuit simulation to evaluate the performance of the PEH with SEC. First, the parameters of the multi-mode ECM are identified from theoretical analysis. The ECM is then established in SPICE software and validated by the theoretical model and finite element method (FEM) with resistive loads. Subsequently, the optimal performances with SEC and CEC are compared considering the practical DC interface circuit. A comprehensive evaluation of the advantageous performance with SEC is provided for the first time. The results demonstrate the feasibility of using SEC as a simple and effective means to improve the performance of a cantilevered PEH at a higher mode.