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( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.
Hong-Guo Liu,Fei Xiao,Hong-Guo Liu 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.12
Two-dimensional arrays of silver oxide nanoparticles were prepared by oxidation of silver nanoparticle arrays in air. The silver nanoparticle arrays were formed by illuminating the composite Langmuir monolayers of nhexadecyl dihydrogen phosphate (n-HDP)/ethyl stearate (ES)/Ag+ at the air-water interface by daylight. The average diameters of the nanoclusters is found to be 2.32 ± 0.89, 2.97 ± 0.78, and 4.94 ± 0.57 nm, respectively, depending on the experimental conditions. X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy and high-resolution transmission electron microscopy (HRTEM) investigations indicate the formation of silver oxide nanoparticles. The possible formation mechanism of the 2D arrays should be attributed to the templating effect of parallel aligned linear supramolecular rows of n-HDP formed at the air-water interface.
Energy Generation Coupled to Azoreduction by Membranous Vesicles from Shewanella decolorationis S12
( Yi Guo Hong ),( Jun Guo ),( Guo Ping Sun ) 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.1
Previous studies have demonstrated that Shewanella decolorationis S12 can grow on the azo compound amaranth as the sole electron acceptor. Thus, to explore the mechanism of energy generation in this metabolism, membranous vesicles (MVs) were prepared and the mechanism of energy generation was investigated. The membrane, which was fragmentized during preparation, automatically formed vesicles ranging from 37.5-112.5 nm in diameter under electron micrograph observation. Energy was conserved when coupling the azoreduction by the MVs of an azo compound or Fe(III) as the sole electron acceptor with H2, formate, or lactate as the electron donor. The amaranth reduction by the vesicles was found to be inhibited by specific respiratory inhibitors, including Cu2+ ions, dicumarol, stigmatellin, and metyrapone, indicating that the azoreduction was indeed a respiration reaction. This finding was further confirmed by the fact that the ATP synthesis was repressed by the ATPase inhibitor N,N`-dicyclohexylcarbodiimide (DCCD). Therefore, this study offers solid evidence of a mechanism of microbial dissimilatory azoreduction on a subcell level.
Wei-Guo Pan,Jie-nan Hong,Rui-Tang Guo,Wen-long Zhen,Hong-lei Ding,Qiang Jin,Cheng-gang Ding,Shi-yi Guo 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4
MnOx–CuOx/TiO2 and MnOx–CuOx/Al2O3 catalysts were prepared by sol–gel method and used for low temperature selective catalytic reduction of NO with NH3. The results showed that MnOx–CuOx/TiO2 had better catalytic activity and SO2 resistance than MnOx–CuOx/Al2O3 in the temperature range of 100–250 ℃. The properties of the catalysts were characterized by using XRD, N2 adsorption, temperature programmed reduction (H2-TPR) and temperature programmed desorption (NH3-TPD). It was found that the support has a great impact on the acidity of catalyst; TiO2 and Al2O3 can promote the formation of Lewis acid sites and Bro¨ nsted acid sites respectively.
Effect of Cu doping on the SCR activity of CeO2 catalyst prepared by citric acid method
Rui-Tang Guo,Wen-long Zhen,Wei-Guo Pan,Yue Zhou,Jie-nan Hong,Hong-jian Xu,Qiang Jin,Cheng-gang Ding,Shi-yi Guo 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4
CeO2–CuO catalyst prepared by citric acid method was investigated for selective catalytic reduction of NO with NH3. The activity of the CeO2 catalyst was enhanced about 8–27% in the temperature range of 125–225 ℃ at a space velocity of 28,000 h 1 by the addition of Cu. It was found that the state of Cu species had great impact on the SCR performance of CeO2–CuO catalyst. Cu2+ can enhance the low temperature activity of SCR reaction, while CuO would promote NH3 oxidation before SCR reaction at high temperature, which would cause the decrease of its high temperature SCR activity.
A 4th-Order Low-distortion Low-pass ΣΔ Modulator Using Timing-Sharing Technique
Hong-guo Zhang,Ji-bao Zhang,Ming-yuan Ren 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.9
A 4th-Order low-distortion low-pass ΣΔ modulator structure is proposed in this paper, which uses the timing-sharing between the 3rd and 4th integrators during one clock phase. Compared with conventional cascade of integrators with distributed feed-forward (CIFF) sigma-delta modulator structure, the proposed structure not only solves the critical timing issue for the quantizer and feedback DAC path, but also eliminates an extra active adder to sum up the input feed-forward. By methods of delay redistribution structure, the time for quantization and feedback dynamic element match (DEM) operation can be extended from a non-overlapping interval for a conventional low-distortion structure to half of the sampling clock period. Capacitive input feed-forward (CIF) methodology eliminates the adder in front of the quantizer. Further, the op-amp of the last integrator is used as an active adder by op-amp sharing. Therefore, the power consumption can be reduced and the linearity of the feedback DAC is improved because of the increasing time which used for implementation of DEM algorism. The proposed 4th-Order low-distortion low-pass ΣΔ modulator with 4-bit quantizer is simulated in MATLAB. From the simulation results, the proposed structure can achieve a peak SNR (signal- to-noise ratio) of 87dB with 2.5MHz bandwidth under 32 oversampling ratio at 160MHz sampling frequency and the ENOB of 14.234bits in non-ideal condition.
A Novel Method of Protection of Monitor Link
Guo Hong-tao 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.1
Automation control systems demand high availability by providing its’ users with reliable and uninterrupted service. Traditional monitor system fails to provide reliable service due to lack of protection of monitor link. It also requires TCP/IP, X.25, E1 and other interfaces to realize monitoring channels such as setting monitored object, reporting status information of monitored call, reporting voice data of monitored call. The organization of the system is very complicated. Besides, some old devices can’t provide all the interfaces. Thus these old devices can’t have access to the lawful interception system. This paper presents a method to provide the ability of monitor link protection under abnormal circumstance. This method offers complete monitor link protection with fewer interfaces. According to the result of experiment and practical application in many countries, the availability and reliability of the interception system have been greatly improved by using this method.
RealCoins : A Case Study of Enhanced Model Driven Development for Pervasive Games
Hong Guo,Hallvard Trætteberg,Alf Inge Wang,Shang Gao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.5
Model Driven Development (MDD) and Domain Specific Modeling (DSM) have been widely used in information system domains and achieved success in many open or in-house scenarios. But its application in the game domain is seldom and immature. In our research, we identified three issues that should be considered carefully in order to play the strength of MDD in the game development environment to a larger extend: 1) structured domain analysis should be done to assure the size and familiarity of the domain; 2) adapted process should be designed to save cost and support evolution; and 3) proper tools (especially language workbenches) should be evaluated and utilized to ease DSM tasks and accelerate iterations. In this paper, we explain these three issues and illustrate our solutions to them by presenting the development details (both technical and procedural) of one pervasive game case. We evaluate the gains and costs by involving MDD into the game development process. We reflect on the issues we have met, and discuss possible future works as well.
Hong Guo,Zisong Bai,Yangyang Xu,Xueqin Wu,Ning Li,Yan Zhu,Xiaoming Wang,Peng Zhang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.7
Pongamia pinnata (Linn.) Pierre has anti-inflammatory activity and could significantly decrease serum tumor necrosis factor-a and IL-10 in arthritic rats. Previous research indicated the typical chemical constituent in P. pinnata is furanoflavone. Guided by anti-inflammatory active assay and UPLC-HRESIMS chromatography, 22 compounds were isolated from the ethanol extract of P. pinnata seedpods. One novel furanoflavone, 40-hydroxypinnatin, was elucidated by HRESIMS, 1D- and 2D-NMR spectra. The 21 known compounds, including 9 furanoflavone, were identified by comparing their NMR data with the previous data in reference. In the known compounds, 5 were isolated for the first time from the species. The anti-inflammatory activities were assayed by assessing LPS-induced NO production in BV-2 cells. 12 compounds can inhibit the production of NO without cytotoxicity at concentration of 50 lM. Among them, compounds 4 can significantly inhibit the production of NO, with the IC50 value of 31.36 lM.