B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과

이찬 ( Chan Lee1 ),장정희 ( Jung Hee Jang ),안은미 ( Eun Mi Ahn ),박찬익 ( Chan Ik Park ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.4

Objectives : Under normal condition melanin protects the skin from extracellular stimuli including ultraviolet (UV)-induced oxidative skin damages, but excess production and accumulation of melanin can induce hyperpigmentation causing esthetic problems. Therefore, in this study we tried to search for natural skin whitening materials from marine natural resources. Methods : Water and ethanol extracts of marine natural resources were prepared from Porphyra thalli (PT), Laminariae thallus (LT), Ostreae concha (OC), Sargassum thallus (ST), Undaria thallus (UT), Codium thalli (CT), Enteromorpha thalli (ET), Syngnathoides biaculeatus (SB), and Hippocampus coronatus (Hc). Their effects against UVB and α-melanocyte stimulating hormone (α-MSH)-induced melanogenesis were investigated based on melanin formation in B16 mouse melanoma cells. The mRNA and protein expression of enzymes involved in the melanogenic process were further examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. Results : Water extract of Ostreae concha (OCW/E) effectively inhibited UVB and α-MSH-induced melanin production in B16 melanocytes, which seemed to be mediated by inhibition of mRNA expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). In another experiment, ethanol extracts from Porphyra thalli (PTE/E), Laminariae thallus (LTE/E), Sargassum thallus (STE/E), Undaria thallus (UTE/E), Codium thalli (CTE/E), Syngnathoides biaculeatus (SBE/E), and Hippocampus coronatus (HcE/E) significantly suppressed UVB and α-MSH-induced melanin formation. Furthermore, ethylacetate fraction isolated form LTE/E (LTE/EEt) decreased UVB and α-MSH-elevated extracellular melanin levels via inhibition of tyrosinase protein expression. Conclutions : These results suggest that marine natural resources such as Porphyra thalli, Laminariae thallus, Ostreae concha, Sargassum thallus, Undaria thallus, Codium thalli, Syngnathoides biaculeatus and Hippocampus coronatus have anti-melanogenic effects, thereby exhibiting high potentials to be utilized as one of the ingredients for the development of new whitening functional cosmetics.

해외 전력거래소들의 전략집단 분석을 통한 한국 전력거래소의 전략방향

이광현,장찬주 국제무역학회 2002 국제무역연구 Vol.8 No.2

우리나라 전력산업구조 개편에 따라 전력산업에 경쟁개념을 도입하게 되었다. 전력산업에 경쟁을 도입한다는 것은 다수의 시장참여자가 존재하는 시장경제체제로의 전환을 의미하는 것이다. 이에 의거하여 새롭게 등장한 한국전력거래소는 경쟁적 전력시장에 참여하는 회원사, 이해관계자 집단 그리고 국민에게 엄청난 영향을 미칠 전망이다. 본 연구에서는 전략집단분석 (Strategic Group Analysis)을 통해 외국의 전력거래소들을 4개의 전략집단으로 그룹핑 하였다. 그리고 한국전력거래소가 지향해야 할 전략집단을 단기·중기차원에서 도출하여 한국전력 거래소의 단지·중기 전략방향을 제시하였다. 더 나아가 우리나라 전력거래소가 지향해야 할 선도 전력거래소들의 구체적 사례분석을 통해 한국전력 거래소가 추진해야 할 향후전략과 핵심역량강화 방안을 제시하였다. Korean government has introduced competition on power industry according to power industry restructuring. To introduce competition on power industry is to transform from monopolistic structure into market-driven system with multiple players. Conseguently, Newly created Korean Power Exchange will have heavy impact on members. interest groups and citizens which participate in the competitive power market. In this research, first, we made four groups of foreign power exchanges by using Strategic Group Analysis. Second, we found short and long term strategic directions of Korean Power Exchange(KPX) based on target strategic group. Third, we made case analysis of foreign leading power exchanges that Korean Power Exchange should follow. Lastly through case analysis. we presented future strategies of KPX and strategies of strengthening core competences.

아메바성 대장염과 연관된 Henoch-Scho¨nlein Purpura 1예

장재현,원경준,이준,변유미,이재광,김영훈,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Henoch-SchonIein purpura is a common form of vasculitis primarily involving the skin, the gastrointestinal tract, the joints, the kidneys, and the nervous system. The etiological investigations are essential, because triggering factors are found in approximately half the patients such as viral, bacterial and parasitic infections, drugs, toxins, systemic diseases and cancer. But it's the correlation between Henoch-SchonIein purpura and Entamoeba histolytica has been rarely reported. We report a 29-years-old woman with Henoch-SchoIein purpura complicated by the lower gastrointestinal bleeding caused by the amoebic colitis. She complained of cramping abdominal pain, lower extremity purpura and hematochezia. Cysts and trophozoites of Entamoeba histolytica were detected in her stool. Skin biopsy demonstrated the leukocytoclastic vasculitis. She was administered metronidazole 1,500㎎ a day for the treatment of amebic colitis. Her hematochezia was improved, but the abdominal pain and purpura were not. The subsequent corticosteroid therapy improved her symptoms.

십이지장과 대장을 동시에 침범한 결핵 1예

이만재,박치영,곽재정,유보열,기장도,박찬국,김만우,김정용 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Tuberculosis of duodenum is so rare and is usually secondary to pulmonary or intestinal tuberculosis . It is difficult to diagnose duodenal tuberculosis in clinical and radiologic findings due to its non-specific symptoms and inconsistent lesion. The diagnosis was made on the basis of the histological study of the endoscopic biopsy and resected surgical specimen. so we report a case of tuberculosis of duodenal bulb and of ascending colon with, the brief review of the literatures.

Na/Si(111)계의 초기 산화과정에 대한 XPS 연구

이득진,안기석,황찬국,장현덕,박종윤 성균관대학교 기초과학연구소 1995 論文集 Vol.46 No.1

X-선광 전자분광법을 이용하여 Na의 흡착에 의해 형성되는 Si(111)7×7-Na과 Si(111)3×1-Na 표면의 초기 산화과정에 대하여 연구하였다. Si(111)7×7-Na 표면은 깨끗한 Si(111)7×7 표면에 비하여 산화가 더욱 잘 일어나지만, Si(111)3×1-Na의 경우 산소와 거의 반응하지 않음을 알 수 있다. 또한 Si(111)7×7 표면과는 달리, Si(111)7×7-Na 표면에 1 ML의 Na를 흡착시키고 산소를 100L 노출한 경우 0 ls 내각 스펙트럼에 두 종류의 산소성분이 존재를 함을 알 수 있다. 산소의 노출량이 증가함에 따라 낮은 운동에너지 성분의 세기는 계속 증가를 하지만, 높은 운동에너지 쪽의 성분은 초기에 포화되었다. 이러한 0 ls 내각 스펙트럼의 변화와 Na KLL Auger peak intensity 증가로부터 Si(111) 7×7-Na 표면에 흡착되는 산소는 Si-0-Si, 그리고 Si-0-Na 두가지의 결합형태로 존재함을 예상 할 수 있다. 또한, Si(111)7×7-Na 표면에 주입되는 산소 노출량이 증가함에 따른 Na KLL Auger peak intensity가 증가하는 현상에 대하여 논의하였다. The initial oxidation of Si(111)7×7-Na and Si(111)3×1-Na surfaces have been studied by means of X-ray photoelectron spectroscopy(XPS). The Si oxidation rate of Si(111)7×7-Na surface was much enhanced more than that of the clean Si(111)7×7, but the Si(111)3×1-Na surface hardly reacted with oxygen. It shows that 0 1s peak of the Si(111)7×7-Na surfaces for 100L O_2 exposure consists of two components, coming from a different oxygen bonding configuration. From the change in intensity of two component of 0 ls and the increase of Na KLL peak intensity with increasing O_2 exposure, it is found that one component originates from Si-0-Si bonding at the lower kinectic energy and the other component comes from the Si-0-Na bonding at higher kinectic energy. Also, we have discussed a possible interpretation for the abnormally increase of Na KLL peak intensity.

자동차용 실리콘 가속도센서의 개발(Ⅱ)

이종현,신장규,이상룡,천희곤,조찬섭,심준환,류인식,박석홍,허정준,박기열 경북대학교 센서기술연구소 1994 연차보고서 Vol.1994 No.-

자동차의 air-bag 장치에 실용될 수 있는 압저항형 단결정 실리콘 가속도센서 칩을 개발하기 위하여 결정 실리콘 미세구조의 제조방법을 확립하고, 단위공정의 검증을 통하여 일괄공정에 의한 PROTO-TYPE 칩을 만드는 기술을 연구하였다. 단결정 실리콘 미세구조는 선택확산법을 이용하여 정확히 선택된 영역에만 air-gap을 형성하여 미세구조의 측면식각을 방지하는 선택확산법에 의한 실리콘 마이크로머시닝 기술로 제조하였다. 일괄공정을 위한 단위공정확립을 위하여 PROTY-TYPE 8빔 브릿지형 가속도 센서를 제조하였다. 제조된 칩의 가속도에 따른 출력전압은 선형성을 나타내고 있으며, 감도는 약 50 ㎶/V·g로 나타났다. 이 감도는 50G용 가속도센서의 사양을 만족하지 못했다. 이는 공정에 의한 문제라기 보다는 가속도센서의 시뮬레이션에 의해 설계한 구조가 이미 원하는 감도에 못 미친다는 것으로 생각된다. 따라서 2차 공정으로 제조될 가속도센서의 파라미터를 SuperSAP 유한요소 패키지를 이용하여 실리콘 미세구조부의 파라미터에 따른 특성을 시뮬레이션하였다. 설계된 50G용 가속도센서의 mass Pad의 반경 및 빔 길이, 빔 폭, 빔 두께, 그리고 mass의 각 파라미터 값은 700 ㎛, 120 ㎛, 5 ㎛, 1.0 ㎎ 이었다. 반도체 공정기술, 관성질량 제조법 및 선택확산을 이용한 마이크로머시닝을 사용하여 일괄공정으로 8빔 브린지형 가속도센서를 제조하였다. We researched the establishment of the silicon microstructure fabrication technique to develop a piezoresistive type silicon acceleration sensor chip and the technique to make a proto-type chip by the verification of the unit-process. Silicon microstructure is fabricated silicon micro-machining by selective diffusion method. This method prevent a side-etching of microstructure because selective diffused region is only formed an air-gap. We fabricated a proto-type 8-beam bridge-type acceleration sensor to establish the unit-process for the batch-process. The output voltage of the chip represented linearity with acceleration, and the sensitivity was about 50 ㎶/V·g. But this sensitivity dosen't satisfy the requirements of a practical acceleration sensor. The cause of this result is assumed not process problem, but the structure designed by simulation isn't suitable already. Threfore, the characteristics of parameters of the acceleration sensor that will be fabricated by 2nd-process is simulated by SuperSAP finite-element package. The determined parameter values of beam length, beam width, beam thickness, mass, and mass radius are 120 ㎛, 5 ㎛, 1.0 ㎎, and 700 ㎛, respectively. We fabricated 8-beam bridge-type acceleration sensor by batch-process using a semiconductor process technique, proof-mass fabrication method, and micromachinig using selective diffusion.

Kalman-Jakubovich-Lemma의 유도와 응용

이광직,박장환 서울産業大學校 1995 논문집 Vol.42 No.1

In this paper it is shown the application and derivation of Kalman-Jakubovich-Lemma for hyper-stability. The Kalman-Jakubovich-Lemma based on passive system, can be applied to SISO(Single Input, Single-Output) system, mutivaliable control system and non-linear control system.

진행성 위암과 동반된 후복막 섬유화증 7예의 임상적 고찰

장재영,천영국,최우봉,김진오,조주영,이준성,이문성,심찬섭 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background/Aims: Retroperitoneal fibrosis is a fibrosing disease process which frequently develops ureteral obstruction. Malignant retroperitoneal fibrosis has been reported that various types of cancer including cancer of the breast, stomach, prostate, lung, cervix, uteri, colon, pancreas, ovary, and even Hodgkin's disease. While the pathogenesis remains obscure, small foci of metastatic neoplasm in the retroperitoneal space can elicit a desmoplastic reaction in secondary form to malignancy. Methods: Recently we experienced 7 cases of retroperitoneal fibrosis with advanced gastric cancer. So, we report 7 cases of retroperitoneal fibrosis with clinical and radiologic characteristics. Results: The most common signs and symptoms were related irreversible renal dysfunction. The urologic finding noted medial deviation of the involved ureters and hydronephrosis Conclusions: Clinical manifestations are abdominal pain, back pain, hydronephrosis, and uremia. When sudden back pain and hydronephrosis develop in advanced malignancy, physicians should be considerd for retroperitoneal fibrosis.

대구지역 가로수잎의 중금속

이찬형,윤현숙,박연준,권종대,노기철,장성환 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

This study was conducted to determine the contents of heavy metals in leaves of roadside trees according to different growth stages in Daegu city. The orders of heavy metal contents in leaves of roadside tree and soil were Fe〉Mn〉Zn〉Pb〉Cu〉Cr〉Cd and Fe〉Mn〉Zn〉Cu〉Pb〉Cr〉Cd, respectively. The contents of heavy metals in leaves of roadside trees and soil showed an increasing tendency as the levels of traffic volume increased. The contents of heavy metals in leaves on October were higher than those on May.

소세포 폐암의 갑상선전이 1예

장리라,최영식,박요한,문대성,김자경,김성은,최인수,유찬희,남성진 고신대학교 의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Metastasis to the thyroid gland is usually considered uncommon. The most frequent sites of the primary tumor include the kidney, breast and lung. Its presence often indicates poor prognosis. Metastases represent the advanced stage of the tumors and fine needle aspiration cytology is and important way of diagnosis in thyroid metastasis. we experienced a case of Metastatic Small cell Lung Carcinoma to the Thyroid Gland. A 58-year-old man with Graves' disease diagnosed pulmonary nodule in the left upper lung field on his chest X-ray film. The patient's thyroid gland was diffusely swollen, and elastic to hard. A thyroid ultrasonography showed 1cm sized hypodense nodule on the left thyroid gland. The patient underwent a ultrasonography guided fine needle aspiration cytology of the mass. Cytological diagnosis was a metastatic small cell carcinoma. Then Chemotherapy was administered to the patient. The authors report this rare case of metastatic small cell lung carcinoma to the thyroid with literature review.