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한우 Band 3 및 CHS 유전병의 분자유전학적 기초연구
정행진,유성란,상병찬,이준헌 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1
Many bovine genetic diseases are currently unidentified in Korea because of the relatively low monitoring systems in the livestock farms. The molecular detection system using PCR-RFLP of two genetic diseases, namely Band 3 (Erythrocyte Membrane Protein Band Ⅲ) and CHS (Chediak-Higashi Syndrome), have been identified in Japan and used for screening large number of cattle whether each individual has the genetic disease or not. Using the 22 unrelated Korean cattle (Hanwoo) individuals, molecular detection system based on PCR-RFLP have been investigated, which can be distinguishable carriers for the genetic diseases. Even though we could not found the causative mutations for two genetic diseases, the PCR-RFLP techniques used in this study are very valuable for the screening the genetics diseases in Korean cattle, especially for the proven or candidate bulls.
요근 농양이 동반된 Salmonella typhi 척추골수염 1예
조윤행,홍성수,김은옥,이미숙,김양수,우준희,배직현,류지소 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2
저자들은 아직 국내에 보고된 적이 없는 S. typhi에 의한 척추골수염 환자에서 ciprofloxacin을 5주간 투여하여 임상적 호전을 보였던 증례를 경험하였기에 보고하는 바이다. Vertebral osteomyelitis by Salmonella infection is rare. Although it is known to be associated with sickle cell anemia, various hemoglobinopathies and immunosuppressive states, it may also occur in normal host. To date, vertebral osteomyelitis with Salmonella. typhi has not been reported in Korea. We experienced a case of spinal osteomyelitis due to S. typhi in a 48-year old man who was admitted because of low back pain for two months. MRI scan showed vertebral osteomyelitis in L1 & L2. Needle aspiration was performed by CT guidance. S. typhi was identified from aspirated pus. However, any organism was not identified from blood and urine cultures. He was treated with ciprofloxacin for 5 weeks successfully.
배택환,김행재,정윤석,김준식,조준필,이철주 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1
Blunt or nonpenetrating chest injuries are common after motor vehicle accidents or falls. Blunt injury to the heart ranges from contusion to rupture. Cardiac rupture, however, is un-common and associated with a very high mortality rate ; left ventricular rupture due to falling is even more rare. We experienced a case of left ventricular rupture of the heart caused by a fall, which the patient survived. A 63-year-old man fell off a ladder and presented with hypotension from left hemothorax and pericardial tamponade. The hemothorax was relieved via an emergency closed thoracostomy but dyspnea and hypotension continued. And so, echocardio-graphy was done and pericardial tamponade was detected. Emergency left thoracotomy was done to repair the rupture site. We believe that patients with cardiac rupture who reach the hospital alive can often be saved by prompt diagnosis and immediate surgical treatment.
조원철,허준행,서규우,이원환 연세대학교 산업기술연구소 1994 논문집 Vol.26 No.1
The water level and flow rate data measured at Indogyo measurement point which serves as one of the major measuring points of Han River have been very important in aspect of controlling the flow. Because these data are becoming more and more unreliable due to the recent change in the basin or the narrowing of the river width, these data will be standardized by comparing the recent data of Indogyo measuring point with the past those. To minimize the errors of water level-flow rate curve caused by the yearly changes of basin, the standard water level-flow rate curve with respect to the present basin conditions will be set, and calculated reduced water level will be compared with the past data. And through frequency analysis, frequency-based flood level with respect to return period will be presented. Finally, the total flow rate of the flood measured in September, 1990 at the point of Indogyo measuring point will be compared with the total flow of the past extreme floods.
Resin Jacket Crown을 이용한 법랑질 형성 부전증 환자의 심미적 치험례
김종수,김용기,이준석,이준행 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.4
Amelogenesis imperfecta represents a group of hereditary conditions that manifest enamel defects without evidence of generalized or systemic disorders. These enamel disorders are apparently heterogeneous in the basic chemical sturcture, resulting in a diverse presentation of clinical characteristics. The reported prevalence of amelogenesis imperfecta varies from 1 in 14,000-16,000 to 1.4 in 1,000 depending on specific population studied with the autosomal dominant hypocalcification type of amelogenesis imperfecta believed to be the least prevalent. The most widely accepted current classification system for delineating the amelogenesis imperfecta types considers the mode ofinheritance and clinical manifestations. Three major groups are recognized; hypoplastic, hypocalcified, and hypomaturation types. Delineating specific types of amelogenesis imperfecta can be confusing due to the phenotypical similarity of many forms and that the most recent classification lists 14 different types. A 12 year-old female patient came to our pediatric dentistry clinic complaining of the ugly shape and color of her teeth, especially the upper front area. Although the goal of the treatment was mainly focused on the improvement of patient's esthetics, longevity of the restorations was also considered in selecting the appropriate restorative system, resin jacket crown, which can satisfy the both aspects.
대식세포에서 산화질소 생성에 대한 當歸 에탄올 추출물의 억제효과
정미영,박히준,정지행,김진용,강전모,이나경,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-
Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and NF-kB activity from RAW 264.7 cells, since NO and nuclear factor-kB(NF-kB) have been shown to be factors implicated In inflammatory disease. Method : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of NF-kB on western blot. Result : LPS-induced RAW 264.7 cells increascd NO production and ,iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced ,iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 μg/ml. LPS-induced RAW 264.7 cells with AGE blocked inhibitory factor-kBα degradation. Conclusion : This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of NF-kB activation in LPS-stimulated RAW 264.7 cells.
이대희,이동호,송인성,정현채,정운태,최규완,김우호,이준행,김정룡,윤정환,임영석,이국래,김병관,황진혁,정준오 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.6
The endoscopic mucosal resection(EMR) is proposed by some Japanese investigators as a curative therapy of early gastric cancer(EGC) because of its minimal invasiveness and excellent results. To evaluate the possible role of EMR as a curative treatment modality of EGC, we retrospectively analyzed l9 casea with EGC initially treated by EMR in Seoul National University Hospital from December 1993 and January 1996. 1) The histologic diagnosis prior to EMR was adenocarcinoma in 12 cases(63%) and adenomatous polyp in 7 cases(38%), which were confirmed as adenocarcinoma after EMR. 2) The histologic curative resection was done in 7 cases(37%). Two cases of them showed recurrence of the gastric cancer and were treated by radical surgery. The other five cases have been closely observed by regular endoscopic examination without recurrence for the maximal period of 1~3 months. 3) Histoiogically inadequate resection(positive cancer cell in resection margin, submucosal cancer infiltration, or no cancer tissue in resected specimen) was done in 12 cases(73%). But two surgically resected cimens of them have no residual cancer cells, and there was no cancer cells in the follow-up biopsy of the other two patients. There 4 cases were seemed to he examples of buring effect of EMR. 4) After excluding five depressed lesions greater than 1 cm is long diameter, nine lesions were curatively treated by EMR. So successful EMR rate for strictly indicated lesions wsa 64%. 5) There was no signifieant complication related to the procedure. We thnnk that endoscopic mucosal resection has a potential role as a curative treatment modality in a highly selected patient with darly gastric cancer.