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한우 Band 3 및 CHS 유전병의 분자유전학적 기초연구
정행진,유성란,상병찬,이준헌 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1
Many bovine genetic diseases are currently unidentified in Korea because of the relatively low monitoring systems in the livestock farms. The molecular detection system using PCR-RFLP of two genetic diseases, namely Band 3 (Erythrocyte Membrane Protein Band Ⅲ) and CHS (Chediak-Higashi Syndrome), have been identified in Japan and used for screening large number of cattle whether each individual has the genetic disease or not. Using the 22 unrelated Korean cattle (Hanwoo) individuals, molecular detection system based on PCR-RFLP have been investigated, which can be distinguishable carriers for the genetic diseases. Even though we could not found the causative mutations for two genetic diseases, the PCR-RFLP techniques used in this study are very valuable for the screening the genetics diseases in Korean cattle, especially for the proven or candidate bulls.
홀스타인종과 한우에 있어서 BoLA-DRB3 유전자의 단일염기다형과 반수체 분석
정행진(Hang-Jin Jeong),유성란(Seong-Lan Yu),라세둘(M. R. Hoque),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),류승희(Seung-Heui Ryoo),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.1
BoLA (bovine leukocyte antigens) have been known as gene complex related with bovine diseases and immunological traits. This study was conducted to find out the characteristics of BoLA-DRB3 gene related to mastitis and BL(bovine leukocyte) from 280 cattle [193 animals of Holstein cattle and 87 animals of Hanwoo]. As a result, five PCR-RFLP types (b, d, e, f and g) using HaeⅢ restriction enzyme, three BstYI restriction patterns (b, d and e) and eight RsaI restriction types(b, d, f, I, j, n, o and w) were identified. Moreover, we identified new d' type (197→175/22), having one more cutting site by BstYI enzyme than d type allele and n' type (180→169/11) having one more cutting site by RsaI enzyme than n allele was additionally identified. Next, we identified 53 SNPs in BoLA-DRB3 exon2 from 280 cattle. SNP frequency and heterozygosity of Holstein and Hanwoo were investigated in all the SNP genotype. These results might be based on research for identifying marker associated with bovine diseases.
홀스타인종 젖소에 있어서 PCR과 ELISA기법을 이용한 BLV 감염진단
정행진(Hang-Jin Jeong),유성란(Seong-Lan Yu),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),서국현(Guk-Hyun Shu),류승희(Seung-Heui Ryoo),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.1
This study was conducted to investigate the farm situation about bovine leukemia virus(BLV) infection that greatly influence productivity in dairy cattle and compare the accuracy of diagnosis for BLV infection between PCR and ELISA techniques. Blood samples of 193 heads from 5 herds in Chungnam and Chungbuk area were used to analyze BLV gene and serum, and the results were obtained as follows. The amplified BLV gene in dairy cattle by PCR technique resulted in 226 bp, 596 bp and 434 bp, respectively, for gag, pol and env, which were well amplified. The infection rates of BLV virus diagnosed by PCR and ELISA techniques ranged from 80.55 to 100% and from 22.22 to 86.95%, respectively, and the infection rates among 5 herds were significantly different in both methods (P<0.05). Further, the average infection rates of 5 herds were 87.05 and 63.21%, respectively, for PCR and ELISA techniques. Kappa statistics for examining consistency of diagnosis by PCR and ELISA techniques showed 0.246, which represents low consistency. Consequently, PCR based BLV technique was considered as a corrective measure for diagnosis of BLV infection in Holstein dairy cattle.
정행진(Hang-jin Jung),서충열(Chung-youl Seo) 한국자동차공학회 2000 한국자동차공학회 지부 학술대회 논문집 Vol.- No.-
The major effective factor on the inner noise of a bus are the structure borne noise and air borne noise. Engine contributes about 80% of the vibration and noise in the vehicle, and exciting forces of the engine are transmitted onto the body through the rubber mount.<br/> This apper aims to investigate the characteristics of engine room walls are insulated, such as<br/> multi layer walls of engine room.<br/> Six kinds of compartment walls are varied with materials and multi layer, depth. Acoustics<br/> characteristics of these partitions, transmission loss, are tested in reverberation chambers.
한국형 홀스타인종 젖소의 BLV env 유전자의 특성분석
정행진(Hang-Jin Jeong),유성란(Seong-Lan Yu),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),서국현(Guk-Hyun Suh),류승희(Seung-Heui Ryoo),정상일(Sang-Il Chung),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2
This study was performed to investigate the characterization of infectious BLV env gene isolated form Korean Holstein Cattle and to determine its incoming origin. Gp51 region of BLV env gene known as having important role in immunological function was characterized using PCR-RFLP sequencing and phylogenetic analysis. BLV env gene was grouped into PCR-RFLP patterns with three restriction endonucleases including Pvu Ⅱ, BamHI and HaeⅢ, and we identified two new RFLP patterns from nucleotide sequences of each group. Phylogenetic analysis showed that 80% of the Korean Holstein was included in the USA and Japanese group. These results here can provide a valuable information about the character of the BLV env gene and research on infection route of BLV.
유성란,정행진,정기철,이준헌,조규완,최재관,나기준,상병찬 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6
Cellular FOS(c-fos) protein is a transcription factor that forms heterodimers mostly with c-jun family and stimulates the transcription of genes containing AP-1 regulatory elements. This c-fos expression can control growth and differentiation of various precursor cells including myoblasts. The controls by c-fos gene have been identified for affecting skeletal muscle fiber traits which are the key determinants of meat quality in pigs. As a first step for identifying the relationship between c-fos gene and meat quality traits in cattle, we fully sequenced 1,443 bp of Hanwoo c-fos mRNA and analyzed expression patterns from various organs and muscle tissues. The sequence identities of Hanwoo c-fos with that of human, pig and mouse showed 89.8%, 93.3% and rib muscle from 7 organs and 9 different parts of muscles investigated. These results presented here can be used as a valuable marker for meat quality related traits in cattle with further verification.
이유주,전얼,정행진,정우영,장병귀,백운기,최강덕,이준헌 한국가금학회 2005 한국가금학회 정기총회 및 학술발표회 Vol.22 No.-
오골계는 중요한 유전자원으로 보존할 가치가 있어 천연기념물로 보호를 받고 있다. 이 품종의 유전적인 조성을 살펴보기 위하여 mitochondrial DNA (mtDNA) 염기서열을 이용하여 계통분석을 실시하였다. 본 연구에 이용된 30마리의 오골계는 4개의 haplotype으로 분리가 되었으며 가장 많은 haplotype에는 11개의 개체가 속해 있었다. 계통 유전학적 분석 결과를 볼 때, 닭은 3개의 그룹으로 나누어지며 오골계는 3개의 그룹 모두에서 관찰됨을 알 수 있었다. 이 결과는 오골계의 보존 및 육종계획을 수립하는데 중요하게 이용될 것으로 사료된다. Korean Ogol Chicken is a natural treasure in Korea and expected to be a valuable genetics resource in the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis has been performed using mitochondrial DNA (mtDNA) sequence variations. Total 30 Korean Ogol Chickens were investigated in this study and they were grouped into 4 haplotypes, consisting 11 birds in the largest haplotype.<br/> Based on the phylogenetic analysis, chicken breeds were divided into three major groups and Korean Ogol Chicken were appeared all of these three groups indicating their large genetic mtDNA diversity. These results will be used for making breeding and conservation strategies for this breed.
한국 Holstein종 유우집단의 DGAT1 유전자의 특성분석
손지영(Ji-Young Son),정행진(Hang Jin-Jeong),유성란(Seong-Lan Yu),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),류승희(Seung-Heui Ryoo),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2009 농업과학연구 Vol.36 No.2
본 연구는 한국형 Holstein종 젖소집단의 DGAT1 유전자의 특성을 구명하고, DGAT1의 유전적 다형과 산유형질인 유량 및 유지량 간의 연관성을 구명하여 젖소집단의 유전적 개량을 위한 분자유전학적 접목을 위하여 실험을 실시하였다. Holstein종의 genomic DNA로부터 PCR기법을 이용하여 DGAT1 유전자좌를 specific primers로 증폭한 후, 1.5% agarose gel에 전기영동한 결과 411 bp의 단편이 양호하게 증폭되었음을 확인하였다. DGAT1 유전자의 염기서열을 분석한 결과 DGAT1 Q 대립유전자의 216-218 bp의 염기서열이 AUG(lysine, K)였으나, 동위치의 대립유전자 q의 염기서열은 GCG(alanine, A)로 치환되어 있음을 확인할 수 있었다. 한편 한국 Holstein종과 NCBI에서 보고된 bovine DGAT1 유전자 단편의 염기서열간에는 100% 상동성을 보였다. 한국 Holstein종 유우집단의 DGAT1 유전자형의 분포는 DGAT1 QQ, Qq 및 qq 유전자 분포가 각각 16.43, 36.43 및 47.14%로 qq 유전자형빈도가 다른 유전자형에 비하여 높았으며, 유전자빈도는 DGAT1 Q 및 q 빈도가 각각 0.35 및 0.65로 q의 빈도가 높았다. DGAT1 유전자형과 산유량인 유량 및 유지량간의 연관성에 있어서는 DGAT1 유전자형이 유량 및 유지량에서 유의적인 차이(P<0.05)를 보였으며, DGAT1 Qq 유전자형이 QQ 및 qq 유전자형에 비하여 유량과 유지량에서 유의적인 차이(P<0.05)로 높은 수치를 나타냈다. This study was conducted to characterize the DGAT1 gene in Korean Holstein dairy cattle population and examine the relationship of DGAT1 polymorphisms with milk yield and milk fat yield for the genetic improvement of Korean Holstein dairy cattle. Results indicated that the 411 bp PCR products were successfully amplified by DGAT1 specific primers. Sequence analysis indicated that the DGTA1 Q allele had AUG (Lysine, K) nucleotide sequences in 216-218 bp and q allele had GCG (Alanine, A) sequences in the same position. Nucleotide sequence homology between the DGAT1 sequences generated in this study showed 100% homology with bovine DGAT1 sequences in the NCBI database. The genotype frequencies of DGAT1 QQ, Qq, and qq were 16.43, 36.43, and 47.14%, respectively, in Korean Holstein dairy cattle population. The observed Q and q allele frequencies were 0.35 and 0.65, respectively. Statistically significant (P<0.05) results were identified for milk yield and milk fat yield for the DGAT1 genotypes. The Qq genotype Holsteins have significantly higher milk yield and milk fat yield than those of the QQ and qq genotype Holsteins(P<0.05).