온주밀감(Citrus unshiu Marc. var. Okitsu) 미분화 종바로부터 callus 유기 및 증식

진성범,홍경애,부경환,이도승,류기중 제주대학교 방사능이용연구소 2000 연구보고 Vol.14 No.-

조직배양을 통한 기관분화나 식물체 재분화 가 어려운 것으로 알려져 있는 온주밀감(Citrus unshiu Marc. var.Okitsu) 미분화 종자로부터 callus 유기 조건을 확립하였다. 온주밀감 성숙과실의 미분화 종자를 MT(Murashige and Tucker, 1969) 기본배지에 치상 6주 후에 미숙종자 양쪽 가장자리에 유기된 조그만white callus를 gibberellin 1mg/L, adenine 25mg/L, malt extract 500mg/L가 첨가된 MT 기본배지에 치상 하여 2주간격으로 계대배양하여 캘러스를 유지할 수 있었고, EME(Grosser and Gmitter, 1990a ) 배지에 4주 간격으로 계대배양함으로써 증식시킬 수 있었다.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

W(110) 표면에 CO의 흡착

이경희,유위량,한현석,부진효,이순보,곽현태 성균관대학교 기초과학연구소 1998 論文集 Vol.49 No.-

The interaction of CO with W(110) surface was investigated through LEED, TDS, and photoelectron spectroscopy using synchrotron radiation under UHV condition. After CO saturation at RT, two desorption states, called α and β , were observed at about 400 and 1150 K in thermal desorption spectra, respectively. The kinetics of 3-CO followed the first order kinetics, indicating the existence of molecular CO on W(110) surface. This is contrary to the previous results. The O 1s BE(binding energy) of CO adsorbed on W(110) surface at room temperature was 529.9 eV. On the other hand, the O 1s BE of β-CO after heating to 900 K was different from that of oxygen adsorbed W(110) surface, suggesting a different adsorption state. According to the UP valence band spectra, we observed two peaks at near -10.7 eV (4σ) and -7.0 eV (5σ+1π), indicating the molecular adsorption of CO at room temperature. Furthermore we could see the 4σ peak at the various photon energy and elevated temperatures. Comparing the energy separation, Δ(4σ-1π) , between 4σ and 1π UP peaks of chemisorbed CO, we found that an increased separation reflects an decreased C-O bond strength. Therefore on the basis of TDS and photoelectron spectroscopy, we could suggest that β state of CO on W(110) may not be dissociated and has an adsorption geometry of lying-down mode.

Paraquat 중독환자의 초기검사로서 sodium dithionite를 이용한 소변내 paraquat검출의 임상적 의의

윤갑준,임경수,이진웅,김영식,이부수,박덕우,김선만,이강현,황성오,안무업 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Mortality from paraquat intoxication depends upon plasma paraquat concentration. To know the severity of paraquat intoxication is important for directing therapeutic modality and predicting prognosis. Sodium dithionite test for urinary paraquat provides an easy and simple method to determine the severity of paraquat intoxication in emergency department. purpose : To determine whether the result urinary paraquat test by sodium dithionite can predict outcome in patients with paraquat intoxication in emergency department. Subjects : 48 patients(male 31, female 17, mean age 37 years) who had exposure to paraquat and presented within 24 hours after exposure. Result : Thirty five patients were positive in paraquat urine test and thirteen patients were negative. Clinical manifestations were more severe in positive patients than in negatives. Complication was much more in positives than in negatives. 28 of 35 patients(80%) in positives and 2 of 13 patients(15%) in negatives died. Conclusion : Positive test for urinary paraquat is associated with high mortality and morbidity from paraquat intoxication, and qualitative test for urinary paraquat by sodium dithionite is an useful method to determine the severity of paraquat intoxication in emergency department.

초음파 유도하 맘모톰 생검을 이용한 병변의 완전제거 : 6개월 추적 초음파 검사를 통해 알아본 유용성

김혜성,한부경,최연현,김지영,고영혜,양정현,남석진,김정한 대한초음파의학회 2004 ULTRASONOGRAPHY Vol.23 No.1

사망진단서(사체검안서) 상의 선행사인으로부터 사망통계의 원사인이 선정되는 비율 : 3개 대학병원에서 교부된 사망진단서를 중심으로

박우성,박석건,정철원,김우철,탁우택,김부연,서순원,김광환,서진숙,부유경 한국의료QA학회 2004 한국의료질향상학회지 Vol.11 No.1

Background : To exatnine the problems intolved in writing practice of death certificates, we cotnpated the determination of underlying cause of death for wital statistics using recorded underlying cause of death in issued death statistics. Methods : We collected 688 rnortality certificates issue in year of 2,000 from 3 university hospitals. And we also collected vital statistics from ministry of statistics. The causes of death were coded by experienced medical record wpecialists. And causes of death determined at ministry of statistics for national vita statistics were mapped to causes of death recorded at each death certificates. The rate that underlying causes of death for vital statistics were derived from underlying causes of death recorded at issued death certificaties sere analysed. Results : 64.5% of underlying cause of death for could be derived from underlying cause of death recorded at issued death certificates, 8.6% derived from intermediate cause of death, and 3.9% derived from direct cause of death. In 23% of cases, underlying cause of death could not be derived using issued death certificates. The rate that underlying cause of death for vital statistics could be derived from underlying cause of death recorded at death certificates was different between 3 university hospitals. Ane the rate was also different between death certificates and postmortem certificates. We classified the causes of death using 21 major categories. The rate was different between diseases or conditions tha caused death too. Conclusion : When we examined the correctness of death certificate writing practice using abpve methods, cortectness of writing could not be told as satisfactory. There was difference in correctness of writing between hospotals, between death certificates and postmortem certificates, and between diseases and conditions that caused death. With this results, we suggested some strategy to improve the correctness of death certificate writing practice.

茶 침출조건에 따른 Catechin, 무기질 및 Vitamin C 함량 변화

이순재,김미지,윤연희,박규영,최원경,방진숙,부용출 대구효성가톨릭대학교 응용과학연구소 1994 응용과학연구논문집 Vol.3 No.-

본 연구에서는 실제 상용하고 있는 각 차음료가 온도, 시간 및 추출 횟수에 따른 성분의 함량의 차이를 관찰코져 시판되고 있는 한국산 녹차, 우롱차 및 홍차를 대상으로 추출방법을 달리한 후 茶浸出液중의 주요성분인 catechin, vitamin C 및 여러가지 무기질의 함량을 측정하여 다음과 같은 결과를 얻었다. 1. 차침출온도에 따른 침출액중의 catechin과 vitamin C 함량의 차이를 볼 때 온도가 높을수록 많이 추출되었다. 2. 같은 온도라는 침출시간에 따른 차이를 관찰한 결과 시간이 길수록 침출액중의 catechin과 vitamin C의 함량이 증가되었다. 3. 녹차는 우려낸 횟수에 따른 catechin의 함량 차이는 처음에 비하여 2회 추출시약 51%, 3회추출시는 71% 감소하였다. 4. 차의 각 무기원소에 따른 함량을 비교시 Mg은 우롱차가 가장 높고 K, Mn은 녹차가, Zn, Cu 그리고 Fe는 홍차가 가장 높게 나타났다. 5. 녹차, 우롱차의 경우 대체로 무기질의 침출정도가 온도가 높을수록 오히려 감소하는 경향을 나타내었고 홍차는 Mg, K을 제외하고 모두 증가하였다. 6. 각 침출액중의 대부분의 무기질 함량은 추출횟수가 증가할수록 감소하였다. 7. 시료로 사용한 녹차, 우롱차, 홍차의 vitamin C 함량은 녹차, 우롱차, 홍차의 순으로 높게 나타났다. 8. 녹차를 추출한 횟수에 따른 vitamin C 함량은 1회에 비해 2회 추출시 약 36%, 3회 추출시 약 62% 감소되었다. 9. 5g의 녹차를 80℃에서 2분간 침출한 액중에서는 catechin은 100ml당 0.34g, Mg은 2.229mg, Ca은 0.365mg, K은 39.77mg, Zn은 0.037mg, Cu은 0.010mg, Fe는 0.001mg, Mn은 0.581mg이며, vitamin C는 9.65mg이 함유되어 있었다. This study was to compare the ingredients which are contained in usual Korean teas in-depednence on leaching temperature, time and number. The main contents i.e. catechin, vi-tamin C and other minerals extracted from Korean commercial green tea, oolong tea and black tea were measured. The resluts are as follows; According to extracting temperature, the higher temperature made catechin and vitamin C extracted more. For the same leaching temperature, the longer time the more catechin and vitamin C. In case of extracting number, the contents of catechin was decreased a-bout 50% and 70% at second time and third, respectively in comparasion to the first time. For minerals from teas, management(Mg) was most in oolong tea, potassium(K)and manganease(Mn) and in green tea and iron(FEe) was in black tea respectively. Most min-erals from greentea and oolong tea were extracted less as leaching temperature was in-creased. But in black tea minerals other than Mg and K were more at increased tempera-ture. Most minerals in extractions were decreased as leaching number was increased. The content of vitamin C in the experimental samples(tea) was most in green tea, next in oo-long tea and third in black tea. In comparasion with the first extraction vitamin C was de-creased about 35% and 60% at 2nd and 3rd leach, respectively.

Health Information Managers’ Job Stress in an Electronic Medical Record Environment

Jin-Won Noh,Hyo-Jin Choi,Jin-Hyuk Hong,Yoo-Kyung Boo 한국콘텐츠학회 2017 International Journal of Contents Vol.13 No.2

Cynara scolymus L. Inhibits the LPS-induced Inflammatory Reaction via Suppression of NF-κB Activity in RAW 264.7 Cells

Kyung-Jun Boo(Kyung-Jun Boo),Kiman Lee(Kiman Lee),Il-Ho Park(Il-Ho Park),Tae Jin Kang(Tae Jin Kang) 대한약학회 2023 약학회지 Vol.67 No.1

Cynara scolymus L. (artichoke) has been traditionally used in the treatment of digestive-related disease, severe hyperlipidemia and liver disease. Recently, anti-inflammatory effect of artichoke has been reported by several studies, but its mechanisms are still unclear. In this study, the anti-inflammatory mechanism of artichoke was studied using an in vitro acute inflammation model. The effect of artichoke on the expression of pro-inflammatory cytokines, such as interleukin- 1beta (IL-1β), IL-6, and tumor necrosis factor-alpha (TNF-α) in murine macrophage cell line, RAW264.7, was examined by using ELISA and RT-PCR. As a result, artichoke inhibited the expression of IL-1β, IL-6 and TNF-α in macrophages activated by lipopolysaccharide (LPS) at a dose dependent manner. The expression of inflammatory mediators such as NO and PGE2 was next investigated in RAW264.7 cells stimulated with LPS. Artichoke also inhibited the production of NO and PGE2 in the cells at a dose dependent manner. Artichoke suppressed the mRNA expression of iNOS and COX-2 in macrophages by LPS. The effect of artichoke on the activation of NF-κB was examined and LPS-induced NF-κB activation was decreased by treatment of artichoke, suggesting that artichoke has anti-inflammatory effect by inhibiting NF- κB activation.

Production of Recombinant Miraculin Protein Using Transgenic Citrus Cell Suspension Culture System

Jin, Seong Beom,Sun, Hyeon Jin,Bachchu, Md Adnan Al,Chung, Sung Jin,Lee, Jongwoo,Han, Song-I,Yun, Jeong Hun,Boo, Kyung Whan,Lee, Dongsun,Riu, Key Zung,Kim, Jae-Hoon The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.3

Miraculin gene containing the N-terminal signal peptide was introduced into navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) callus cells by Agrobacterum-mediated transformation. Transgenic somatic embryos were screened on the shoot induction medium containing 25 mg hygromycin $L^{-1}$. Citrus callus cells were reproduced from the green color somatic embryos on the callus reproduction medium. The obtained transgenic cells were cultured in Murashige and Tucker's liquid medium containing 50 g sucrose $L^{-1}$ in a shaking incubator. Similar to the native miraculin, the secreted recombinant miraculin protein formed a disulfide-linked dimer and retained taste-modifying activity. The stability of recombinant protein expression was confirmed over nine generations of callus. This production system can be an excellent alternative for producing various recombinant proteins as well as miraculin.