http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
온주밀감(Citrus unshiu Marc. var. Okitsu)과 오렌지(Citrus sinensis (L.) Osbeck)의 원형질세 융합
홍경애,송성준,이옥영 제주대학교 방사능이용연구소 2000 연구보고 Vol.14 No.-
By the development of plant molecular biology these days which has been accomplishing much an attempt to get mutants. However, there have been haying many issues in lacking of recognition for transformants and the right of ownership of the gene. The breeding method of cell fusion without any problems can easily make new variety with an excellent character having plant itself. The purpose of this study was therefore to carry out to get trantsformants having sweeter taste and the original character of Citrus unshiu itself using PEG method of cell fusion. The yield of protoplasts per gram of leaf was 7 × 105 ~ 1 × 106/㎖ and callus cell was 1 × 106 / ㎖ . The first protoplast division and formation of micro-calli were observed 7 to 9 days and 30 to 40 days after incubation, respectively. To induce embryo from these calllus were inoculated solid medium containing 1.6% agarose and 5% sucrose. Green somatic embryo and plantlet were appeared from 1 to 2 months after plating and transfered to MT basal medium to stimulate rooting and shoot elongation. It was difficult to get normal plant directly through embryo of globular, heart and terpenoid state, and more effective method was directly to get plantlet from the globular embryo without heart and terpenoid state to control properly agar concentration.
Phytochrome이 보리잎 Protoplast의 팽윤에 미치는 영향
홍경애,송성준,송필순,유장걸,문두길 제주대학교 방사능이용연구소 1986 연구보고 Vol.2 No.-
암조건에서 생육시킨 보리(Hordeum valgare)잎의 생장점 부근에서 분리한 Protoplast가 적색광 처리에 의해서 팽윤되었고 적외선광에 의해서는 적색광 효과과 소멸되었다. 이 사실은 Protoplast의 팽윤이 Phytochrome에 의해서 조절됨을 시사하는 것이나 Phytochrome의 작용기작을 알기 위해서는 다각적인 연구가 필요하다. 실험결과로 부터 적색광 처리시간은 2분 적색광 처리후 배양시간은 2시간이 적당함을 알았다. Protoplast 배양액의 Sorbitol 농도에 따른 크기변화는 농도가 낮을수록 커졌으며 0.4M 이하에서는 Protoplast가 파괴되었다. Light effect on the size of protoplasts isolated from etiolated barley leaves was studied. Red light irradiation caused a swelling of protoplasts while little change in size found under the dark condition. Photoreversibility by red light and far-red light was found in protoplast swelling, indicating the involvement of phytochrome. Action mechanism of phytochrome on protoplast swelling should be clarified in aspect of growth regulators and intermediates involved. Two minute irradiation time and two hour incubation after red light treatment were found sufficient to induce protoplast swelling. The protoplasts were swollen according to the sorbitol concentration of incubation media but destroyed below 0.4M-sorbitol concentration.