한우 myostatin 유전자의 SNP 및 발현분석

유성란,정기철,상병찬,이준헌 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.2

Myostatin is a transforming growth and differentiation factor-β family member that acts as a negative regulator of muscle growth. Previously, mutations in the myostatin gene were known to be related to double muscling phenotypes in cattle. Because myostatin gene is highly related to muscle mass, also meat quality, in cattle, we sequenced whole myostatin mRNA and investigated the SNPs (Single Nucleotide Polymorphisms) in Korean cattle (Hanwoo). The results indicated that Hanwoo had an SNP in nt2385 and this mutation can be a useful marker with further verifications. We also investigated expression patterns of the myostatin gene from various muscle tissues and organs. Northern blotting results indicated that myostatin expression was restricted in muscles with variable expression levels. The results presented here can be used as a valuable information for meat quality related traits and muscle mass in cattle.

제 3형 구상돌기 골절의 수술적 치료

전인호,민우기,오창욱,경희수,박병철,김풍택,인주철,이정엽 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적: 8례의 제 3형 척골 구상돌기 골절에 대해 수술적 치료 후 임상적 방사선학적 결과를 보고하고자 한다. 대상 및 방법 : 8명의 구상돌기 골절에 대한 후향적 연구를 시행하였다. 대상은 모두 남자였으며 평균 나이는 33세였다. 3례에서 척골 구상돌기 단독 골절이었으며 2례에서 주관절 탈구가, 2례에서 요골 두 및 경부 골절이, 1례에서 내측 측부인대 손상이 동반되었다. 전례에서 전방 도달법을 통해 유관나사를 이용한 관혈적 정복 및 내고정이 시행되었다. 평균 추시 기간은 31개월 이었으며 (24~60개월), Mayo Elbow Performance Score로 임상적 결과를 판정하였다. 결과 : 최근 추시에서 평균의 능동적 주관절 가동범위는 105°였다. 평균의 Mayo Elbow Performance Score는 76.9점이었다(50~95). 1례에서 최우수, 4례에서 우수, 2례에서 양호, 1례에서 불량의 결과를 보였다. 결론 : 제 3형의 척골 구상돌기 골절에서 조기의 관혈적 정복 및 안정적인 내고정으로 신뢰할 만한 치료를 제공하였으며, 손상 주관절의 동반된 인대손상과 복합골절은 중요한 예후인자로 간주되었다. Purpose: To review the clinical results of eight cases of typeⅢ coronoid process fractures which were treated operatively. Materials and Methods : Eight patients with coronoid type Ⅲ were reviewed retrospectively. All were men with an average age of 33. There were three isolated fractures, two elbow dislocations, two radial head and neck fractures, and one medial collateral ligament rupture. Open reduction and internal fixation through anterior approach with canulated screws was used. The patients were followed up for a mean of 31 months(24 to 60). Results: Average active elbow joint motion at the most recent follow up was 105°. The average Mayo Elbow Performance Score was 76.9 (50 to 95). There was one excellent result, four good, two fair, and one poor. Conclusion: Early open reduction and stable internal fixation provided a reliable method for the treatment of typeⅢ coronoid process fractures. Any associated ligament injuries to the elbow and fracture comninution were considered as important prognostic factors.

퍼지이론을 이용한 구획배치 평가 기법에 관한 연구

김수영,신성철,박준규,박정기 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.58 No.-

본 연구는 선박의 내부 공간에 대한 여러 구획 배치안들 중에서 최적의 배치안을 찾기 위해 배치안들을 평가하는 것을 목적으로 한다. 이런 공간에 대한 구획 배치 고려사항에 영향을 주고 컨트롤하는 두 가지 요소는 기능 구획간의 연관성과 공간이 위치하게 되는 환경이다. 여러 배치안들을 평가하기 위한 단계에서 수식화 하기 어려운 각종 설계 정보 및 이들 간의 상관관계를 파악하고 처리하기 위하여 퍼지 이론을 적용하는 기법을 제안한다. This study is objective to layout evaluation to find an optimal layout of the some compartment layouts in internal spaces of ship. In this compartment layout considerations about spaces, two factors which control and affect are association between functional compartments, and environment that space is located. At level to evaluate some layouts, we suggest method that applies to fuzzy theory to conduct and understand every design informations difficult formulating and their relationship.

대전광역시 양축농가의 축분뇨 관리 실태 및 VOCs 농도 조사

이봉덕,이수기,오홍록,허정민,정기철,김성복 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

This study was carried out to investigate the style of livestock house, concentration of malodorous substances of livestock feces and livestock houses in Daejeon area. Among the livestock houses investigated, as most of cow pens(94.5%) have sawdust or chaff on the bottom, there was no leakage of feces out of pen. Most pig pens adopted slury style, but some of them currently use buffering material on the bottom. It is thought that there will be no possible contamination leakage. When it comes to hen house, all the broiler house use litters on the bottom and all the layer house use scrapper. It is also thought that there will be no possible contamination leakage. 3 out of 12 deer pens used buffering material on the bottom, 10 places were maintained in a traditional method, and 7 places left possibility of contamination leakage considering whether the roof was installed or not. The contents of ammonia, amine and volatile fatty acid in fresh feces were lower compared to rotten feces, but the concentration of sulfur-containing matter - hydrogen sulfide, methylmercapthan and ethylmercapthan were higher compared to rotten feces. In the case of malodorous ingredient in livestock houses, only small amount of ammonia and hydrogen sulfide were detected in pig pen and hen house, and other ingredients were not detectable. And those who are engaged in animal husbandry reacted negatively to the use of feed additives for decreasing malodor. In conclusion, it is not worrisome that contamination can be leaked out of animal raising facilities. But if we take into consideration that the point of investigation time is wintry season, there should be more considerate attitude. And feed additives for decreasing malodor need establishing criteria in the manufacturing process.

Insertional variation of two Porcine Endogenous Retroviruses(PERVs) in Korean native pigs and Asian Wild Boar

JUNG, KIE-CHUL,YU, SEONG-LAN,KIM, TAE-HUN,PARK, CHANG-SIK,JIN, DONG-IL,ROGELGAILLARD, CLAIRE,CHARDON, PATRICK,MORAN, CHRIS,LEE, JUN-HEON 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Identification of meat quality related genes in Korean Native Chicken using Proteomics

Kie-Chul Jung,Kang-Duk Choi,Byoung-Gui Jang,Byung-Don Sang,Jun-Heon Lee 한국가금학회 2003 한국가금학회 정기총회 및 학술발표회 Vol.20 No.-

본 연구는 proteomics의 방법을 이용하여 가금의 육질과 관련된 단백질을 찾고자 수행하였다. 인삼부산물 추출액을 급여한 실용 재래닭에서 육질과 관련된 것으로 추정되는 후보 단백질들이 선발되었으며, 이 결과는 가금의 육질을 향상시키는 단백질 Marker로서 이용될 수 있을 뿐만 아니라 현재까지 밝혀지지 않은 가금의 2D map을 만드는데 역시 중요하게 이용될 수 있다. There is growing interest for improving meat quality in chicken. Recently, the proteomics can be used as a valuable tool for identifying candidate proteins. In this study, we investigated the proteins expressed in chicken muscle for obtaining chicken muscle reference two dimensional(2D) map and identifying the proteins in muscle affecting Ginseng diet. A few candidate proteins have been currently characterizing using MALDI - TOF Mass spectrometry. Further investigations of the proteins can be used as valuable markers for selection of better quality chicken meat.

Insertional variation of two Porcine Endogenous Retroviruses (PERVs) in Korean native pigs and Asian Wild Boar

JUNG, KIE-CHUL,YU, SEONG-LAN,KIM, TAE-HUN,PARK, CHANG-SIK,JIN, DONG-IL,ROGELGAILLARD, CLAIRE,CHARDON, PATRICK,MORAN, CHRIS,LEE, JUN-HEON 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Tropomyosin and triosephosphate isomerase are upregulated proteins affecting Ginseng treatments in chicken muscle

Kie-Chul Jung,Kang-Duk Choi,Byoung-Gui Jang,Byung-Don Sang,Jun-Heon Lee 한국가금학회 2004 한국가금학회 정기총회 및 학술발표회 Vol.21 No.-

본 연구는 proteomics의 방법을 이용하여 가금의 육질과 관련된 단백질을 찾고자 수행하였다. 인삼부산물을 급이한 실용재래계와 대조구와의 비교에서 육질과 관련된 3개의 후보 단백질이 이 연구를 통해 밝혀졌다. 이 유전자들은 tropomyosin, triosephosphate isomerase와 한 개의 기능이 밝혀지지 않은 단백질이었다.<br/> 각각의 기능을 살펴볼 때 이 유전자들은 근육의 성장과 면역의 증강에 관여하며 이 결과는 인삼부산물을 이용하여 가금의 육질을 향상시키는 단백질 마커로 중요하게 이용이 될 수 있을 것으로 추정된다. The present study was aimed to investigate proteome affected by Panax ginseng extracts in chicken muscles. More than 300 protein spots were detected on silver staining gels. Among them, four protein spots were distinctively up-regulated by Panax ginseng treatments. The up-regulated proteins were finally identified as tropomyosin (2 spots), triosephosphate isomerase, and one unknown protein. Based on the known functions of the identified proteins, they are highly related to the muscle development and enhanced immunity in chicken. These proteins can give valuable information of biochemical roles for Panax ginseng in chicken meats.

DC 배전용 3상 AC/DC 컨버터의 병렬 운전시 발생하는 순환전류 제어 알고리즘

정철호(Chul-Ho Jung),신수철(Soo-Cheol Shin),정태복(Tae-Bok Jung),이택기(Taeck-Kie Lee),원충연(Chung-Yuen Won) 전력전자학회 2011 전력전자학술대회 논문집 Vol.2011 No.11

한국 재래닭의 MHC 영역 유전자형 분석

정기철(Kie Chul Jung),라세둘(Rashedul Hoque),서동원(Dong Won Seo),박병권(Byung Kwon Park),최강덕(Kang Duk Choi),이준헌(Jun Heon Lee) 韓國家禽學會 2010 韓國家禽學會誌 Vol.36 No.4

닭의 MHC 유전자 내에 있는 microsatellite marker LEI0258은 혈청의 구분 및 질병 저항성 유전자로 많은 연구가 되어 있다. 본 연구는 유전변이가 있는 microsatellite marker LEI 0258을 한국 재래계 흑색종, 한국 재래계 갈색종, 코니쉬종, 로드아일랜드 레드종에 적용하여 품종 특이 유전자형 및 allele을 탐색하고, 품종 구분 활용에 기능하지 여부를 실험하기 위하여 실시하였다. 한국 재래계만이 가지고 있는 특이 대립 유전자는 찾을 수는 없었지만 품종간 유전자형의 빈도 차이를 보이는 대립 유전자들을 확인할 수 있어 마커의 조합을 통하여 품종을 구분할 수 있는 가능성을 제시하였으며, 질병 저항성 연구의 기초 자료로 그 이용성이 높을 것으로 사료된다. The chicken major histocompatibility complex (MHC) is known to be associated with disease resistance and susceptibility to several pathogens. The microsatellite marker LEI0258 is physically located between the BG and BF of MHC region and variations near this marker have been well documented. In this report, the LEI0258 marker was used to find specific alleles for the Korean native chicken. The MHC haplotype was analyzed by PCR screening and sequencing of LEI0258 region in four different breeds including black Korean native chicken, brown Korean native chicken, Cornish and Rhode island red. The serologically same MHC haplotypes showed the differences in repeat numbers, a few indels or single nucleotide polymorphisms by sequencing analysis. Even though we could not identify specific alleles for Korean native chickens, the genotypes analyzed in these breeds can give valuable information for the relationships with disease resistance and establishment of breeding strategies for the Korean native chicken.