Oualbain과 Verapamil이 심근칼슘저장고에 미치는 영향

김병국,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1981 中央醫大誌 Vol.6 No.1

The control isometric contraction of rabbit papillary muscle preparations were recorded by the stimulation 'frequency of 1 Hz. and priming stimulation were applied to activate the intracellular calcium by the stimulation frequency of 5 Hz. for 5 seconds. After the resting period, the amplitudes of contraction, were compared with that of control and the ratios were plotted with respect to time in a semi-log paper. The experiments were performed in a control Tyrode solution, high calcium solution, ouabain solution and verapamil solution. The following results were obtained; 1. la a normal Tyrode solution, the fast compartment which is responsible for the fast decay of intracellular calcium within 3-5 seconds after the priming stimuli and the slow compartment, slowly decayed one after 5 seconds, could be identified. 2. Although the fast components showed no significant differences between those in a normal Tyrode solution and in a high calcium (4mM) solution, the slow components showed a rather increasing tendency during the normal initial decay period up to 20 seconds and later on a decreasing tendency. 3. In a ouabain, 2.4 -4.8×10^-7M, treated preparation the amplitude of contraction during the control period was increased up to 1.5 times compared with that in a normal Tyrode solution and the characteristics of calcium pools were qualitatively similar to those in high calcium solution. 4. In a verapamil, 1.1×10^-6M, containing solution the amplitude of control contraction were reduced to two thirds compared with that in normal Tyrode solution and the fast component were markedly influenced, reversed its slope to increase slowly up to 20 seconds and then decayed. 5. The characteristics of intracellular calcium pools la a preparation of verapamil and high calcium conditions at the same time showed rather similar patterns with those in verapamil alone treated preparation. The above results showed that the intracelluar calcium pools were composed of two components which were fast and slow decaying ones, and that ouabain and verapamil would alter the characteristics of the calcium pools to increase and decrease the intracellular calcium respectively.

광음향분광법에 의한 AI_2O_3:Cr 결정의 Cr^3+이온의 비복사천이과정 연구

남상돈,최복원,문병기 부산교육대학교 과학교육연구소 1985 科學敎育硏究 Vol.10 No.-

Photoacoustic spectroscopy is applied to study the nonradiative processes of Cr^3+ions in Al_2O_3:Cr^3+ crystal at room temperature. Three spectra, photoacoustic spectrum, luminescence excitation spectrum and optical absorption spectrum, are compared. It is shown to be possible to identify the modes of the relaxation processes of Cr^3+ ions in crystal by comparing photoacoustic spectra with luminescence excitation spectra. Furthermore, it is shown that the photoacoustic spectroscopy can be used to identify the forbidden transitions and the spin doublet energy levels of Cr^3+ ions in Al_2O_3:Cr^3+. These experimental data are in good agreement with the crystal field calculations. The relaxation process from ^2T_1 or ^2T_2 to ^4A_2 is dominantly nonradiative. However, the relaxation from either ^4T_1 or ^4T_2 to ^4A_2 is consist of the nonradiative relaxation process(^4T_1→^2Eor^4T_2→^2E) followed by the radiative relaxation process(^2E→^4A_2)

문자영상 紬線化에서의 雜音除去 알고리즘에 관한 연구

전병민,박규태,김상운,이기돈 연세대학교 산업기술연구소 1984 논문집 Vol.16 No.2

In this paper, we study thinning algorithms which are peeling off successive contours of the character image to be thinned while keeping the connectivity of the skeleton in the process. Noise rejection algorithm is proposed for the purpose of removing the common short-comings of thinning algorithms when applied to hand-printed data. The algorithm consists of the three processings: preprocessing, thinning, and postprocessing. The preprocessing smooths down the originally-thick image and the postprocessing leads to the well-groomed skeleton. The noise rejection algorithm is implemented with FORTRAN language and tested through a CYBER170-825 computer system.

동방결절의 전기적특성에 미치는 Higenamine의 효과

이상돈,엄대용,조성일,방효원,윤병만 중앙대학교 의과대학 의과학연구소 1987 中央醫大誌 Vol.12 No.2

The rabbit sinoatrial node was cut into small pieces which wew 0.5×0.5㎜ in sizes, electrically insulated and could be partitioned into central and peripheral areas. Glass microelectrodes were impaled into both areas and transmenbrane potentials were recorded in order to observe the changes in effects of higenamine on the both areas by the addition of TEA, Cs^+ and Ba^2+ or pretreatment of propranolol. 1. Effects of higenamine were more prominent in high K^+ concentration than those in the low K^+ concentration. 2. TEA induced the decrease in the spontaneous firing rate and the prolongation in the action potential duration which could be reversed by higenamine. 3. Ther were prominent decrease of spontaneous firing rate and the prolongation in the action potential duration in peripheral area with the uncrease of Cs^+ concentration. Higenamine reversed the decrease of spontaneous firing rate in some degree. 4. There were significant decreases of spontaneous firing rate, amplitude of action potential and maximum diastolic potential by the Ba^2+ in peripheral area. Effects of Ba^2+ were reversed by higenamine in central area. 5. The effects of higenamine were completely blocked by the pre-treatment of propranolol. 6. There was no effect of higenamine of the Na-K pump activities. The above results could be interpreted as effects of higenamine were mediated by the chamge of calcium ion current rether than those of the potassium ion current in sinoatrial nodes.

多層薄膜型 ZnS : Mn電場發光素子에 관한 硏究 Mn Thin Film Electroluminescence and it's Characteristics

李相潤,羅炳旭,孫相豪,李相敦 慶北大學校 1986 論文集 Vol.42 No.-

The devices have an ITO-Y_2O_3-ZnS : Mn-Y_2O_3-Al structure with Y_2O_3 double insulating layers. The dependence of the emission characteristics and crystallinity of ZnS : Mn upon the fabrication parameters such as the Mn concentrations and substrate temperatures during the evaporation has been investigated. The highest brightness with 30fL at frequency of 1KHz and a pulse height of 200V was obtained. And then the peak of spectrun of PL and EL are 5850Å.

개구리 정맥동(Sinus Venosus)의 전기적 성질에 관한 연구

안병규,엄대용,이상돈 중앙대학교 의과대학 의과학연구소 1983 中央醫大誌 Vol.8 No.1

The frog sinus venosus were studied with conventional glass microelectrode technique in order to elucidate the underlying mechanism of spontaneous pacemaker activity. The analyses were focussed on the ionic nature of pacemaker current by changing the concentrations of extracellular K^+ and/Na^+, or by using blockers of K- and Ca-current and chronotropic transmitters. 1. The action potential sinus venosus has some characteristic feature of maximal diastolic potential ranged from -65 to -75mV, resting potential from -45 to -50 mV and overshoot voltage about +20mV, respectively. Duration of the action potential taken from rapid upstroke to maximal diastolic potential was about 600 msec. Usual discharge rate was 30~35/min at room temperature(18~20℃). 2. In Ringer solution containing 50% Na(substituted by equimolar Tris) reduction of extracellular sodium concentrations diminished the amplitude and frequency of the action potential. And 0 and 11 mm Na hyperpolarize the restal membrane potential greatly. It suggest the resting Na Conductance is significantly high in sinus venosus. 3. Caesium(10mM), K-current blocker decreased pacemaker depolarization. Manganese(2mM) known to be Ca-current antagonist, blocked spontaneous activity completely. 4. Adrenaline and acetylcholine showed positive and negative chronotropic effect. It is concluded that K-, Na- and Ca-current components are involved in the genesis of spontaneous activity of the frog sinus venosus like other cardiac pacemaker tissues.

소아 만성 부비동염에서 단순촬영상과 OMU CT상의 비교

이병돈,박상호,김장묵,이승균,장혁순,강주원,김장욱 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

Most commonly and widely used diagnostic tool for chronic paranasal sinusitis in children is plain X-ray check not only because of small radiation dose and easy performance, but because of low cost. However ostiomeatal unit computed tomography(OMU CT) was known to be more accurate and recommended recently by many physicians. The author have studied for both OMU CT and plain X-ray of paranasal sinuses and compaird their findings in 90 chidren(180 sides of sinus) aged from 4 to 14 years who suspected having sinusitis. OMU CT was taken after plain X-ray examination (Caldwell's view, water's view, lateral view), and the time interval betweeen these two examination was O to 14 days (average 4.7 days). The following results were obtained. Considering that OMU CT was accurate, the rate of correspondence in diagnosis of sinus pathology between plain X-ray and OMU CT was 48.3% in the ethmoid sinus, 51.1% in the maxillary sinus, 41.9% in the sphenoid sinus, 43.4% in the frontal sinus. If all OMU CT findings had been accurate, the incidence of false positives according to plain X-ray was 25.5% in the ethmoid sinus, 28.3% in the maxillary sinus, 70.8% in the spenoid sinus and 77.8% in the frontal sinus. While, the incidence of false negatives according to plain X-ray was 85.9% in the ethmoid sinus, 70.5% in the maxillary sinus, 50.0% in sphenoid sinus and 37.5% in the frontal sinus. A routine preoperative OMU CT is recommended, which allows a detailed evaluation of pathologic changes and anatomical relations of the paranasal sinuses before surgical interventiaon for the paranasal sinuses. Considering the above facts, diagnosis of pediatric sinusitis only by plain X-ray was inadequate and OMU CT was necessary.

백혈병과 과립구 감소증에서의 감시배양(surveillance culture) : 예방적항균제 사용에 따른 균총의 변화

최강원,김성민,오명돈,김병국,김의종 대한화학요법학회 1991 대한화학요법학회지 Vol.9 No.1

A surveillance culture was to monitor the changes in flora during prophylactic antibiotics(PA) in patients whith granulocytopenia. Twenty patients with granulocytopenia were included: divided into 3 PA groups. Terimthoprim-sulfamethoxazole(TS) group consisted of 7 patients who underwent induction chemotherapy, Cipprofloxacin plus nystatin(CN) group consisting of 7 patients and Gentamicin plus Nystain(GN) group consisting of 7 patients. All of the latter 2 group underwent bone marrow transplantation and were put in protected environment (laminar air flow, sterile food) in addition to PA. Normal flora and other colonizing microorganisms were replaced with antibiotic resistant bacteria and fungus : MRSA, methicillin resistant CNS, P. aeruginosa, Enterobacter spp. and candida spp. and truolopsis. Entreobacteriaceae were diminished markedly, expecially with CN. Two episodes of bacteremia were preceded by the colonization with the same organisms in the skin, throat, and stool. Surveillance culture seems useful in the helping to design prdventive measures, select antibiotics n selected cases.

삼출성 중이염 환자에서의 혈중 및 아데노이드 면역글로불린 E의 면역혈청학적 연구

장혁순,강성호,유창수,김성현,이병돈,박영진,윤세동,안희영,강주원 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Otitis media with effusion (OME) is one of the most common disease in children but its pathogenesis is not clear. In particular the role of allergy has revealed conflicting support with widely varing opinions as to its importance. Within recent years, striking biochemical and immunochemical analytic techniques have opened a new approach the study of otitis media with effusion. So we focused on distributions of IgE in adenoid tissue and serum IgE levels. Authors compared the serum IgE and the distribution of IgE in adenoid tissue between the 20 patients underwent tonsillectomy, adenoidectomy and ventilating tube insertion and control group of 11 patients underwent tonsillectomy and adenoidectomy. The results were as follows; 1. In the distribution of IgE in adenoid tissue, there was a significant increase in the group of patients with OME compared to the control group (P<0.01). 2. In the analysis of serum IgE levels, the levels of IgE in the OME patients an control group were higher than normal level, although the level of IgE in the OME patients were less than that of control group, there was no significance P>0.05).

Small Cell Lung Cancer Cell Line을 이용한 Xenoplanted nude mice에서 방서선 치료후 종양의 변화 관찰에 관한 연구

김동욱,유명상,김재욱,이병돈,장혁순 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.1

Recently, combination of ionizing radiation with inhibitors of angiogenesis has been reported to improve tumor eradication compared to treatment with irradiation alone. However, the mechanism of this effect have not been defined. For this pupose we established a non-small cell lung cancer model in nude mice. Tumor vascularization was visualized in vivo by MRI using gadolinium-DTPA as contrast agent. Further, cryosections were produced exactly in the MRI slice positions. Since we were interested to examine formation of recurrent tumor irradiation was performed with a single fraction of 6 Gy. This dose caused a partial remission followed by recurrent tumor growth 25 to 35 days after therapy. The process of partial remission as well as formation of the recurrent tumor was examined in 35 nude mice analysing the following parameters: (1) contrast agent enhancement using high-resolution MRI, (2) proliferation of tumor cells and fibroblast using Ki-67 immunohistochemistry, (3) formation of microvessels using CD31 immunohistochemistry. The latter analyses lead to differentiation of three stages. Stage 1(day 1 to 15 after irradiation) was characterized by increasing area of dead cell mass in hematoxylin-eosin stained slides that corresponded to a decrease in tumor cellproliferation as well as contrast agent enhancement. The percentage of Ki-67 positive tumor cells decreased from initially 45.1 ±6.0 to 1.4 %±1.2 % on day 15. Stage 2(days 6 to 20 after irradiation; overlapping with stage 1) was characterized by proliferation of fibroblast leading to formation of fibrotic septae with abundant microvessels. Already during late stage 2 MRI identified new contrast agent enhancing areas. Stage 3(day 20 to 40 after irradiation) was characterized by new tumor cell proliferation. Interestingly, tumor cells almost exclusively proliferated in the direct neighbourhood of the fibroblasts and blood vessels was a condition prior to foramtion of recurrent tumor tissue. Thus our results are in contrast with the view that tumors or recurrent tumors begin as avascular masses that later induce neovascularization. With respect to clinical practice our results suggest that (1) adjuvant anti-angiogenic therapy should not be limited to the day of irradiation but should cover a critical period until day 5 to day 20 after radiotherapy, (2) adjuvant therapy should also include inhibition of fibroblast proliferation, (3) MRI can identify a recurrent tumor 10 to 15 days before occurrence of new tumor growth.