허혈성 흰쥐 대뇌에서 NMDA 수용체 아단위2A와 중간세사섬유들의 변화

정용욱,문일수,고복현 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

대뇌피질의 지연성 신경손상과 NMDA수용체 아단위2A, NF200K, GFAP의 표현 변화와의 관계를 알아보기 위해 전반적 저산소증(92% N2/8% O2)과 불완전 국소허혈(왼온목동맥 결찰)을 시행하였으며 허혈성 손상이 후 대뇌 피질에서의 NR2A,NF200K,GFAP의 변화는 immuno blot방법과 면역조직화학법을 이용하여 관찰하여 다음과 같은 결과를 얻었다. 1)허혈 손상 3일 후 대뇌 피질 PSD에서의 NR2A항체에 대한 면역 반응성은 정상에 비해 증가하였 으며 NF200K의 면역 반응성은 감소하였다. 2) 허혈 손상 6일 후 대뇌 피질 PSD에서의 NR2A, NF200K항체에 대한 면역 반응성은 함께 감소 하였다. 3) 면역조직화학법에서 NF200K,GFAP에 대한 중간세사섬유들의 표현은 3일 후 정상에 비해 함께 감소 하였다가 6일 후 허혈의 정도에 따라 증가군과 감소군으로 나누어졌다. 이상의 결과를 볼 때 대뇌 피질 PSD에서 NF200K는 3일 이전에는 NR2A의 양에 의해 조절되며 6일 이후에는 NR2A, NF200K 모두 손상되는 과정을 거치는 것으로 생각된다. 또한 별아교세포의 증식은 신경세포 손상을 방지 하기위해 보상적으로 증가하는 것으로 생각 된다. Ischemic brain cortex has been developed to understand the relationship between delayed neuronal damage at the cerebral cortex regions and the expression of NMDA receptor subunit (NR2A), NF200K and GFAP in the conditions of global hypoxia and focal left common carotid artery ligation. Changes of NR2A, NF200K and GFAP in rat brain after ischemic injury were investigated through immunoblot analyses and immunohistochemistry. We found that immunoreactivity to NR2A antibody in the cerebral cortex PSD was up-regulated and recovered to normal by 3 and 6 days while NF200K was down-regulated by 3 and 6 days. Immunoreactivity to anti-NF200K and GFAP antibody was decreased at the third day but by sixth day they were varied on the degree of ischemic damage. These resulys imply that the amount of NR2A and NF200K in cerebral cortex PSD at the third day were reversely changed but by the sixth day all of them were decreased in the process of delayed neuronal damage and that, neuroprotective proliferation of astrocytes may be compensated by the elevation of neuronal cell damage.

T형 합성보의 스터드 쉬어 콘넥더의 내력에 관한 연구

박복만,정구섭,최승용,김복순 명지대학교 공학기술연구소 1993 공학기술연구소 논문집 Vol.8 No.-

This study summarizes the results of tests on 18 Two-slab push out specimens. The main purpose of the investigation was to evaluate the capacity and behavior of stud shear connectors embedded in the normal concrete(F_c=173∼295㎏/㎠). Two different diameters(Φ16mm,Φ13mm) of steel shear connectors welded on T-shpaed steel were used for push out specimens. The following conclusions were drawn from this study. 1. The shear strength(q_u) of stud shear connectors embedded in concrete was influenced by the cross sectional area of the steel shear connector(A_s), concrete design strength(F_c) and elastic modulus of the concrete(E_c). The emperical function described the test results. q_u = 0.3A_s√FcEc 2. The maximum loads in this study were reached at slips varing from 1.5mm to 4.0mm 3. The webs of T-shaped steels welded by the steel shear connectors were safe under t≥d/2. Where t is the thickness of the webs, d, the diameter of stud shear connector.

溫室保溫커튼의 材料特性에 따른 保溫效果分析

金榮福,金容換,李昇揆,金成泰,羅又禎,閔永鳳,朴重春,崔東烈 慶尙大學校 1991 論文集 Vol.30 No.2

The effects of the material properties of thermal curtains upon reduction of the greenhouse heat loss were studied. A theoretical analysis procedure with equations and computer programs was developed to estimate them by using energy balance principles. Curtain effectiveness for every three kinds pf value level of the material properties were studied and compared. The results of this study would be useful for the developent and management of greenhouse thermal curtains.

^(7)Li, ^(87)Rb과 ^(27)Al NMR을 이용한 (K_(1-x)Li_(x))_(2)SnCl_(6), (K_(1-x)Rb_(x))_(2)SnCl_(6)와 K_(2)SnCl_(6):Al^(3+)의 상전이 근처에서의 결정 동력학 연구

김복수,김영훈,강재필,백아영,차유정,김민경,서용문,송승기 명지대학교 자연과학연구소 2004 자연과학논문집 Vol.23 No.-

K_(2)SnCl_(6)는 antifluoride계에 속하는 결정으로서 K_(2)SnCl_(6)에 Li^(+)를 첨가하여 ^(7)Li 핵자기공명(NMR)을 측정하여 동형(isomorphic) 혼합결정(K_(1-x)Rb_(x))_(2)SnCl_(6)와 비동형(nonisomorphic) 혼합결정 K_(2)SnCl_(6):Al^(3+)에서의 핵자기공명(NMR), ^(87)Rb NMR과 ^(27)Al NMR의 측정값과 비교하였다. Rb^(+) 이온은 K_(2)SnCl_(6)의 K^(+)이온을 대신해 동형 결정을 이루며, Al^(3+)이온은 Sn^(4+)이온을 대치하면서 비동형 결정을 이루는데 Li^(+)이온 또한 K^(+)이온을 대신하는 동형 혼합결정과 유사한 결과 값을 얻을 수 있었다. 이 연구 결과는 종래의 Cl-NQR 연구 결과와 비교함으로서 K_(2)SnCl_(6)의 동력학적 특성과 구조 상전이 근처에서의 임계현상을 새로운 관점에서 볼 수 있는 가능성을 보여주었다. ^(7)Li, ^(87)Rb and ^(27)Al NMR have been investigated in the isomorphic and nonisomorphic impurity doped crystals (K_(1-x)Li_(x))_(2)SnCl_(6), (K_(1-x)Rb_(x))_(2)SnCl_(6) and K_(2)SnCl_(6):Al^(3+) as a supplementary to the previous Cl NQR studies in these crystals. The results yielded that the features of NMR spectra for both types of crystals are roughly in line with the systematic trends shown in the Cl NQR in these crystals, though the outstanding characteristics exhibited in the Cl NQR become in the case of the NMR of the doped impurities in crystals somewhat weakend. This is due to the degradation of the role of the local field from the main hamiltonian in NQR to the subsidiary in NMR. Each NMR result of the iso/nonisomorphic crystals has been analyzed in terms of the 'impurity induced dynamic lattice defects' for K_(2)SnCl_(6):Al^(3+) and the 'static primary impurity effects' for (K_(1-x)Rb_(x))_(2)SnCl_(6) and (K_(1-x)Li_(x))_(2)SnCl_(6) respectlvely.

Polaron Hopping in the K_(2)O · B_(2)O_(3) · V_(2)O_(5) Glasses

차유정,강재필,홍성덕,김복수,김영훈,서용문,송승기 명지대학교 자연과학연구소 2005 자연과학논문집 Vol.24 No.-

K_(2)O · B_(2)O_(3) · V_(2)O_(5) 계 유리의 조성비에 따른 밀도, 굴절율, 전이온도, 천이금속이온비율 등과 직류 전기 성질을 100~450K 온도영역에서 연구하였다. 실험결과를 비정질 반도체에 관한 전기전도에 대한 이론 모델을 이용하여 분석하였다. 고온 영역에서는 직류전도도의 온도 의존성을 Mott의 포논-도움껑충뛰기 전도모델로 분석할 수 있었다. 활성화에너지는 0.28~0.33 eV로 시료내 V_(2)O_(5) 의 양이 증가할수록 감소하는 경향을 보인다. 저온 영역에서 직류 전기전도도는 Mott의 가변거리 껑충뛰기(variable-range hopping)전기전도도와 잘 일치하며, N(E_(F))값은 국지화 상태에서와 같다. The dc conductivity of K_(2)O-B_(2)O_(3)-V_(2)O_(5) glasses has been investigated in the temperature range 100~450K and physical parameters such as density, refractive indices, glass transition temperature and fraction of reduced transition-metal ion, were observed for various compositions. The experimental results are analyzed with reference to theoretical models proposed for electrical conduction in amorphous semiconductors. The analysis shows that at high temperature region the temperature dependence of the dc conductivity is consistent with Mott's model of phonon-assisted hopping of small polarons. The values of activation energy are 0.28~0.33 eV and decrease with increasing V_(2)O_(5) content in the sample. The lowtemperature dc conductivity data can be well fitted to Mott's variable-range hopping conductivity and the values of N(E_(F)) are also reasonable for localized states.

퇴행성슬관절염 환자에 있어서 관절경수술의 효과

강수용,최호림,정영복 대한슬관절학회 1991 대한슬관절학회지 Vol.3 No.2

Recently arthroscopic examination and operation have replaced open arthrotomy surgery and become one of good treatment modahties in osteoarthritis of the knee which does not respond to conservative treatment. Arthroscopic debridement was carried out in 52 knees of 48 patients who had a primary diagnosis of osteoarthritis at Chung-Ang University Yong-San Hospital from January 1988 to September 1990. The patients ranged in age from 34 to 83 years with mean age of 54.9 years (male: female= 1: 1.8) and the average follow up period was 16.5 months from 12 to 36 months. Following results were obtained; 1. Arthroscopic lavage, meniscectomy, synovectomy, cartilage drilling and loose body removal were performed with overall results of 3 excellent (6%), 28 good (54%), 14 fair (27 %) and 7 fail (13 %). 2. The result of gynovectomy was most satisfactory for 2 excellent, 7 good and 2 fair. In conclusion, arthroscopic debridement can be one of the good alternative treatment methods of osteoarthritis and a time saving procedure before arthroplastic surgery of the knee.

충북 남부지역 중소 제조기업의 경쟁력 제고를 위한 경영 및 애로기술 실태조사 연구

윤상원,구경완,정성진,백기영,김선훈,서용성,이근복 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 연구에서는 충북 남부 3개군 지역(옥천 · 보은 · 영동)의 중소 제조기업의 경영실태 및 애로기술을 대상으로 실증조사 되었다.·지역의 특정상황의 전제하에 지방 중소기업들의 실질적인 문제점들을 발견하고, 그 개선 방안을 탐색함은 물론 산 · 학 · 연 컨소시엄 사업의 성공적인 수행을 위해 시도되었다.세부적으로는 중소 제조기업의 경영 및 애로기술 특성을 일반적 사항, 경영애로요인, 경영전략, 현장개선기법, 정보화, 제품 및 공정 기술상의 애로기술 현황파악의 6개 영역으로 조사되었다. 조사결과들은 추후 산 · 학 · 연 공동기술개발 사업에 있어 창의적, 틈새적 과제발굴 및 신제품 기술개발의 토대마련이 될 뿐 아니라, 지역경제 발전을 위한 산 · 학 관의 지원 전략 수립에 적극 활용 될 것으로 판단된다.

기니픽에서 Kanamycin 이독성

박찬일,송영복,정봉진,박용호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

To evaluate experimental kanamycin - induced ototoxicity, changes of hearing loss were observed after administration of kanamycin in guinea pig, using auditory brainstem rcsponse(ABR) audiometry. Kanamycin(KM), 50mg/kg/day(group I), 100mg/kg/day(group Ⅱ), 200mg/kg/day(group Ⅲ) and 400mg/kg/day(group IV), was injected intramuscularly in each group and ABR thresholds were measured every other days through 50 days after the initial administration of KM. The injection of KM was discontinued at the day when the ABR threshold was increased more than 40 dB in each guinea pig. After the discontinuation of KM injection, ABR thresholds recovered progrssively to the base line level before the day 50 in group I and Il. But the ABR threshold changes were progressed after discontinuation of KM injection and finally they showed no responses in group III and IV. The results suggested that high dose KM administration could result progressive and irreversible hearing loss after the discontinuation of KM administration.

만성 심부전 환자에서의 Denopamine 투여효과

최석구,김현대,정태기,김대현,김용복,유원상 인제대학교 1994 仁濟醫學 Vol.15 No.2

만성 심부전 환자에게 새로운 경구용 비 digitalis 강심제인 denopamine 1T(5mg)을 1일 3회 3개월간 투여하여 그 유효성 및 안전성을 검토했다. Background : Patients with congestive heart failure have a high mortality rate and are also hospitalized frequently. We studied the effects of a new oral inotrophic agent, denopamine in patients with chronic heart failure. Methods : 16 patients receiving conventional treatments for heart failure were treat ed with denopamine 5mg tad for at shortest 3 months. Their history were taken and the physical examination, general laboratory test and the echocardiographic examination were done before and after treatment. Results: There were 3 deaths and 1 withdrawal because of worsening heart failure. 5 patients (42%) among 12 patients showed the improvement in their subjective symptoms. The fractional shortening and ejection fraction of the left ventricle were increased by 3.2% and 5.6% respectively in their absolute values. Conclusion : The addition of denopamine to conventional therapy considerably improved the subjective symptoms and the left ventricular function in the limited number of patients with chronic heart failure.

제주도 양식넙치, Paralichthys olivaceus의 스쿠티카충병 발생 동향

진창남,이창훈,오상필,정용욱,송춘복,이제희,허문수 한국어병학회 2003 한국어병학회지 Vol.16 No.2

The research was carried out to know the occurrence trend of scuticociliatosis and mortality of fingerlings in flounder. The diseased fish were collected from on-land rearing farms and hatcheries of flounder in Jeju Island form January 1991 to December 2001. Occurrence of scuticociliatosis was about 10% of total occurred diseases for 1991 to 1996, but increased to 34.5% in 2001. Although monthly occurrence of seuticociliatosis was not related to season from 1999 to 2001, the highest occurrence was revealed in July and August. Mixed infections of bacteria including Vibrio sp., Edwardsiella tarda, Streptococcus sp., and Flexibacter sp., with seuticociliates were observed. Among the bacteria, Vibrio sp., was the highest frequency(40.1%), and the lowest one(10.1%) was Streptococcus sp., Mortality of fingerlings by seuticociliates was different sharply according to size of fish. Mortality of infected fingerlings below 7㎝ of total length was 53.8%, while that of normal fingerling above 7㎝ of total length was 13.2%. When fish were grown above 25㎝, mass mortality by seuticociliates infection was decreased markedly.