「후성 유전이 마음에 미치는 영향」에 대한 논평

문일수 한국불교상담학회 2022 한국불교상담학회 학술대회지 Vol.17 No.-

중추신경계 연접내 열충격 단백질 hsp70 의 동정 : 연접후 치밀질내 해석후 기전의 존재?

문일수 한국생명과학회 1998 한국생명과학회 학술발표회 Vol.20 No.-

형성평가 시스템에서의 개별 피드백

문일수,문창배,김한일 朝鮮大學校 電子情報通信硏究所 2003 電子情報通信硏究所論文誌 Vol.6 No.2

일반적으로 형성 평가는 교수-학습 활동이 진행 중에 투입되는 평가활동으로 학습 정보에 대한 피드백과 교정 및 교수 방법의 개선을 위한 평가이다. 본 논문에서는 학습자 개인의 특성에 따라 서로 다른 피드백을 제공하는 형성평가 시스템을 제안한다. 이 시스템은 지식, 이해, 적용 등의 영역별 기준에 따라 제작된 문항을 통해 각 학습자를 평가하고 이 결과를 기반으로 구분된 학습자 유형에 따라 서로 다른 피드백을 개별 학습자에게 제공하며 학습자는 이를 통해 부족한 영역을 보완하게 된다. 교수자에게는 문항별 정답 비율뿐만 아니라 학생 개개인에게 제공되었던 피드백 내용도 알려주어 교수방법을 개선하도록 한다. Generally speaking, the formative evaluation, an evaluative activity inputted in the process of teaching and learning, is aimed at bringing forth the feedback and correction on learning information and the improvement in teaching methods. In order to provide the different feedback drawing on the individual learner's characteristics, the system offers him or her the formative evaluation items, which are produced on the basis of the criteria by sections such as knowledge, comprehension and application, and it divides the feedback types according to the assesment result. Each student is given the different feedback depending on each feedback type, and he or she can make up for lacking student as well as the itemized correct answer ratio, and improve the teaching methods.

기억형성의 연접기전 : 연접하부위에서의 단백질합성 Protein Syntheisis at Subsynaptic Sites

문일수,고복현 東國大學校醫學硏究所 2004 東國醫學 Vol.11 No.1

기억은 신경망의 형성으로 이루어지는 것으로 받아들여지고 있다. 장기기억의 형성에는 연접부위에서의 단백질합성이 필수적인 것으로 알려져 있다. 이러한 연접부위에서의 단백질합성을 위하여는 mRNA가 특정 부위에 수송되어야 하며, 단백질합성은 국소적으로 조절되어야 한다. 연접이 생성되는 가지돌기에는 mRNA와 RER polyribosome, rRNA등 일부 인자들이 존재한다. mRNA는 RHA granule에 싸여 연접하부위에 수송되는데 여기에는 translation elongation factor (cEF)와 initiation factor (eIF)는 없는 것으로 알려져 있다. 필자는 연접후세포막에 단백질이 모여 있는 연접후치밀질(PSD)의 단백질구성을 조사하던 과정에 cEF1A HSP70, eIF들이 존재함을 알았다. 이들은 가지돌기내 국소적 단백질합성에 관여할 가능성이 높다. 가지돌기내 국소적 단백질합성은 활성-의존적 연접가소성의 변화에 중요한 역할을 하며, 결국 기억형성에 매우 중요하기 때문에 여기에 대한 최근의 연구결과를 소개한다. Memory is believed to be formed by neural nets. Long-lasting memory requires new proteins in addition to the pre-existing ones in the synapse. The new proteins are synthesized locally at the activated synapse. For this to occur, -As are transported to synaptic sites and local protein synthesis must occur indepent of each synapse. Dendritic mRNAs are transported in RNA granules which are translationally incompetent, because they lack translation elongation factors such as eEF and eIF. We found that eEFlA, HSP70, and eIF are present in the spines. These proteins are likely to play roles in local protein synthesis. In this review, we summarize recent development in local protein synthesis and propose a model for a mechanism of synapse-specific protein synthesis.

공무원 교육을 위한 시청각교구활용의 제문제

문일수 대한지방행정공제회 1966 地方行政 Vol.15 No.147

N-Acetylglucosamine Kinase is Localized to Dendritic Lipid Rafts and Caveolae of Rat Hippocampal Neurons

문일수,Moon, Il-Soo Korean Society of Life Science 2006 생명과학회지 Vol.16 No.6

A dynamic cycle of addition and removal of O-linked N-acetylglucosamine (O-GlcNAc) at serine and threonine residues is emerging as a key regulator of nuclear and cytoplasmic protein activity. In this work, immunocytochemistry was carried out to investigate the subcellular expression of GlcNAc kinase (NAGK, EC 2.7.1.59) that catalyzes the phosphorylation of GlcNAc to GlcNAc 6-phosphate. Immunostainings of cultured rat hippocampal neurons revealed patchy or punctate distribution of NAGK. When NAGK is doublestained with caveolin-1 or flotillin, markers for caveolae and lipid rafts, respectively, NAGK was co-localized with these markers. These results indicate that most, if not all, of the NAGK immunopunctae represent caveolae and lipid rafts, and suggest NAGK's role in these membrane microdomains. 단백질의 serine 및 threonine 잔기에 O-linked N-acetylglucosamine (O-GlcNAc)의 수식은 핵단백질과 세포질 단백질의 주요 조절인자로 부각되고 있다. 본 연구에서는 GlcNAc를 인산화시켜 GlcNAc 6-phosphate로 만드는 GlcNAc kinase (NAGK, EC2.7.1.59)의 세포내 표현을 면역화학적 방법으로 조사하였다. 배양한 해미신경세포에서 NAGK는 가지돌기를 따라 점박이(punctae)를 형성하였으며, 이 점박이들은 caveolin-1 혹은 flotillin 항체에도 염색이 되었다. 이들은 각각 caveolac와 lipid raft의 표지단백질이기 때문에 본 연구결과는 NAGK가 세포막의 이러한 특수 미세부분(microdomain)에 존재함을 의미하며, 이 미세부분에서 아직 알려지지 않은 어떤 기능을 할 것을 시사한다.

Dendritic eIF4E-binding Protein 1 (eIF4E-BP1) mRNA Is Upregulated by Neuronal Activation

문일수,Hyung Jong Lee,In Sick Park 대한의학회 2012 Journal of Korean medical science Vol.27 No.10

Long-term synaptic plasticity requires addition of new proteins at the synaptic site. The local protein synthesis at subsynaptic sites confers advantageous mechanisms that would regulate the protein composition in local domains on a moment-by-moment basis. However, our information on the identities of ‘dendritic’ mRNAs is very limited. In this study we investigated the expression of the protein and mRNA for eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4EBP1) in cultured rat hippocampal neurons. Immunocytochemistry (ICC) showed that 4EBP1 protein is highly localized to the nucleus. In dendrites most 4EBP1 punctae were not colocalized with those of eIF4E. In situ hybridization (ISH) and Fluorescence ISH (FISH) revealed that 4EBP1 mRNA was present in dendrites. The FISH signals formed clusters along dendrites that colocalized with ICC signals for Staufen, a marker for RNA granules. The neuronal activation by KCl (60 mM, 10 min)significantly increased the density of 4EBP1 FISH signals in the nucleus after 2 hr, and both in the nucleus and dendrites after 6 hr. Our results indicate that 4EBP1 and its mRNA are present in dendrites, and the mRNA is upregulated and transported to dendritic domains in RNA granules upon neuronal activation.

Neuronal activation increases the density of eukaryotic translation initiation factor 4E mRNA clusters in dendrites of cultured hippocampal neurons

문일수,Sun-Jung Cho,Dae-Hyun Seog,Randall Walikonis 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.8

Activity-dependent dendritic translation in CNS neurons is important for the synapse-specific provision of proteins that may be necessary for strengthening of synaptic connections. A major rate-limiting factor during protein synthesis is the availability of eukaryotic translation initiation factor 4E (eIF4E), an mRNA 5'-cap-binding protein. In this study we show by fluorescence in situ hybridization (FISH) that the mRNA for eIF4E is present in the dendrites of cultured rat hippocampal neurons. Under basal culture conditions, 58.7 ± 11.6% of the eIF4E mRNA clusters localize with or immediately adjacent to PSD-95 clusters. Neuronal activation with KCl (60 mM, 10 min) very significantly increases the number of eIF4E mRNA clusters in dendrites by 50.1 and 74.5% at 2 and 6 h after treatment, respectively. In addition, the proportion of eIF4E mRNA clusters that localize with PSD-95 increases to 74.4 ± 7.7% and 77.8 ± 7.6% of the eIF4E clusters at 2 and 6 h after KCl treatment, respectively. Our results demonstrate the presence of eIF4E mRNA in dendrites and an activity-dependent increase of these clusters at synaptic sites. This provides a potential mechanism by which protein translation at synapses may be enhanced in response to synaptic stimulation. Activity-dependent dendritic translation in CNS neurons is important for the synapse-specific provision of proteins that may be necessary for strengthening of synaptic connections. A major rate-limiting factor during protein synthesis is the availability of eukaryotic translation initiation factor 4E (eIF4E), an mRNA 5'-cap-binding protein. In this study we show by fluorescence in situ hybridization (FISH) that the mRNA for eIF4E is present in the dendrites of cultured rat hippocampal neurons. Under basal culture conditions, 58.7 ± 11.6% of the eIF4E mRNA clusters localize with or immediately adjacent to PSD-95 clusters. Neuronal activation with KCl (60 mM, 10 min) very significantly increases the number of eIF4E mRNA clusters in dendrites by 50.1 and 74.5% at 2 and 6 h after treatment, respectively. In addition, the proportion of eIF4E mRNA clusters that localize with PSD-95 increases to 74.4 ± 7.7% and 77.8 ± 7.6% of the eIF4E clusters at 2 and 6 h after KCl treatment, respectively. Our results demonstrate the presence of eIF4E mRNA in dendrites and an activity-dependent increase of these clusters at synaptic sites. This provides a potential mechanism by which protein translation at synapses may be enhanced in response to synaptic stimulation.

Brain Science of SATI Practice 싸띠명상의 뇌과학

문일수 한국불교상담학회 2021 한국불교상담학회 학술대회지 Vol.15 No.-

투고 : 지방청 공무원교육에 있어서 교수계획과 교육방법 - 공무원교육을 중심으로 -

문일수 대한지방행정공제회 1966 地方行政 Vol.15 No.150