유세포 측정을 통한 CD34^(+)세포의 정량방법 비교

양형모,서영훈,엄현석,민창기,김희제,정대철,이종욱,진종율,한치화,김춘추,김학주 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.2

본 연구소에서는 현재 CD34^(+) 세포의 정량에 사용되고 있는 4가지 서로 다른 방법을 가지고 골수에 적용하여 그 결과를 비교하였다. 각각의 방법들이 편리한 점과 불리한 점들을 가지고 있었지만, 통계적으로는 유의한 차이를 보이지 않았다. 1) CD34-PE/CD45-FITC 방법13(ISHAGE: International Society of Hematotherapy and Graft Engineering), 2) CD34-PE/CD14-FITC 방법, 3) CD34-PE/CD14-FITC/CD45-PerCP 방법, 4) DNA dye/CD34-PE/CD45-PerCP4(Becton Dickinson) 등을 비교하였으며 그 결과 CD34^(+) 세포의 농도는 다음과 같았다. 1) 0.473±0.345, (%Mean±SD, N=70) 2) 0.663±0.519, (N=20), 3) 0.543±0.381 (N=46), 4) 0.624±0.341, (N=32). 시료로는 여의도 성모병원 카톨릭 조혈모세포이식 센터에서 채혈한 골수를 이용하였다. Four different methods for flow cytometric quantitation of CD34+ cell were compared using bone marrow samples (n=168). Following combination of antibodies were used for the analysis : A) CD45-FITC/CD34-PE (n=70), B) CD14-FITC/CD34-PE (n=20), C) CD14-FITC /CD34-PE /CD45-PerCP(n=46), D) DNA dye/CD34-PE/CD45-PerCP (n=32). Each method showed no significant differences among them (P=0.126, ANOVA test, SPSS Version 7.5). When we compare these methods using same samples (n=12), the P value became 0.832. The mean % of CD34+ cells calculated in this experiment were A) 0.474%, B) 0.663%, C) 0.548%, D) 0.624% respectively. In our laboratory, we decided to use CD45-FITC/CD34-PE methods as a routine protocol.

재발성 류마티즘 환자에서 발작의 유발인자

주유철,이동민,양종태,신병철,임태영,서정균,정기영,최동헌,김원,조영신,고희관 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : Palindromic rheumatism (PR) is known to be triggered without any obviously inciting events in most patients, although, in a few cases, attacks have been noted to relate to another events such as weather change, childbirth, ingestion of certain foods, or over-exercise. The aim of this study was to characterize the triggering factors on palindromic attacks. Materials and Methods : We evaluated sixty-four patients with PR for the presence of triggering factors and the relationship between attacks and triggering factors by interview of patients. We considered that there was an probable association between episodes and PR, if episodes within 24 hours prior to PR had triggered at least 50% of all attacks and a definite association between episodes and PR, if episodes within 24 hours prior to PR had triggered 100% of all attacks. Results : In 15 patients (35.9%), there was a possible association between episodes of PR and initiating events. In 8 patients (12.5%), there was a definite association between episodes of PR and initiating events. The triggering factors were physical over-activity including exercise (19/64: 29.7%), foods, such as rawfish, crab, and chicken, including alcohol (19/96: 19.8%). However, there were no difference between the groups with and without the triggering factor in sex ratio, duration and onset of PR, the positive rate of rheumatoid factor and involved sites. Conclusion : Our observations showed that PR was triggered by physical over-activity more than in previous reports and hypersensitivity to foods was a causative factor in some patients with PR. However, patients with triggering factors had a similiar clinical profile to patients without triggering factors.

영아 천식의 임상적 고찰 : Asthma prospective score에 의한 Based on Asthma Prospective Score

양은석,노영일,문경래,박영봉,박상기,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

A Clinical study was made on 40 patients for infantile asthma less than 2 years old who had been admitted to the Department of Pediatrics of Chosun University Hospital from January 1997 to December 1998. We evaluated family history of allergic diseases, clinical features, serum IgE level, eosinophilia, chest radiologic findings and response to bronchodilator. The results obtained were as follows ; 1) The male to female ratio of patients studied was 4 : 1. 2) Eighteen of the 40 patients (45.0%) had a family history of allergic diseases, of which 10 patients had maternal allergic diseases. 3) Atopic dermatitis was the most common disease (37.5%) among the associated allergic diseases. 4) Thirty two of the 40 patients (80.0%) showed high serum total 19E levels. 5) Only 5 of the 20 patients who were examined by MAST showed positive reaction. 6) The frequency of chest radiologic findings observed in these patients was as follows ; hyperinflation (52.5%), increased bronchovascular marking(30.0%), no pathologic findings(25.0%), and pneumonic infiltration(25.0%). 7) In response to bronchodilator 28 cases(70.0%) were effective. 8) When we divided the 40 patients into two groups by the diagnostic criteria of Asthma prospective score (AP score), 30 patients(75.0%) were included in a compatible group. On the basis of AP score, family history of major or minor allergic dieases and the responsiveness to bronchodilator were important factors for the diagnosis of asthma.

성인 막성신병증의 임상적 고찰

양종오,이상주,박기현,장윤경,이강욱,서광선,신영태 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Membranous nephropathy (MN) is a glomerular disease characterized by diffuse thickening of the glomerular basement membrane without significant mesangial proliferative change and is usually manifested by the nephrotic syndrome. To evaluate the clinical and pathologic characteristics of this disease in adults, we analyzed 65 patients with primary membranous nephropathy who were diagnosed at Department of Internal Medicine, Chungnam National University Hospital from February 1986 to February 2001. The results are as follows: 1) Of total 96 patients with membranous nephropathy, 67.7% was primary MN. Hepatitis B-associated MN and lupus MN were 22.9%, and 9.4%, respectively. Median duration of follow-up was 35.1 months(0.3∼177) in primary MN, 49.6 months(2.5∼103) in lupus MN, and 35.6 months(0.5∼108) in hepatitis B-associated MN. 2) Mean age of the patients with primary MN at the time of diagnosis(43.3 years) was significantly higher than that of lupus MN(30.4 years) and HBV-associated MN(36.2 years). Male to female ratio of primary MN was 1.03:l. 3) At the time of diagnosis, 89.2% of primary MN was presented with nephrotic syndrome. Twenty seven percent of patients showed hypertension. Hematuria was found in 58.5% of primary MN patients. Azotemia was noted in 3.1 %. 4) Global sclerosis, tubular atrophy and interstitial fibrosis were noted in 39.8, 16.1 and 11.3% of primaty MN, respectively. IgG and C3 were deposited on the glomerular capillary loop in 92.7% and 29% of primary MN patients. There was no significant correlation between the electron microscopic pathologic stage and clinical findings. 5) Of 56 patients with primary MN followed more than 6 months, 50(90.9%) patients were treated with prednisolone, cyclophosphamide or cyclosporin. Complete remission was obtained in 29 patients(51.8%), partial remission in 6 patients(10.7%), and no response in 18 patients(32.1%). Of 6 patients who were not treated with immunosuppressive drugs, 3 patients showed spontaneous complete or partial remission. Three patients who did not show clinical improvement progressed to end-stage renal failure. There were no significant clinical difference including renal pathology at the time of diagnosis between the patients who showed complete or partial remission and patients who did not. In conclusion, it was difficult to predict the long-term prognosis of primary MN at the time of diagnosis, clinically. The response to therapeutic modality would be the most important to predict long-term prognosis of primary MN.

본태성 고혈압이 동반된 당뇨병환자의 혈중 인슐린 농도

양동호,홍세용,성기범,안무영,윤신구,박형국,양광익,황주호,신현길 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Multiple lines of evidence link elevated blood pressure with diabetes mellitus. Specifically, it has been proposed that resistance to insulin-stimulated glucose uptake and hyperinsulinemia may play a central role in the cause and clinical course of hypertension. In diabetes mellitus, insulin resistance and/or hyperinsulinemia is an common finding and it is interesting whether the hyperinsulinemia may play a signigicant role in hypertension with DM in the same way as in essential hypertension without diabetes mellitus. The object of this study was to compare insulin and c-peptide levels between age, sex, and obesity matched two groups(DM with DM without hypertension). Method The study group consisted of 55 male diabetes mellitus patients, aged between 45-55 (years). Patients with obesity (body mass index · 30 ㎏/m2), renal disease (proteinuria · 300 ㎎/24hr urine), and secondary hypertension were excluded. Insulin and c-peptide were measured in overnight fasting state and after oral administration of glucose(75 gm). In the fasting, venous plasma glucose levels were similar in the hypertensive and control group(132 ±7 ㎎/dl vs 135 ±8 ㎎/dl). In the fasting, venous plasma insulin levels were higher in the hypertensive than in the control group (10.9 ±5.3 μIU/ml vs 5.5 ±3.9 μIU/ml, p = 0.0001). After loading with 75 gm glucose, venous plasma insulin level seems to be higher in the hypertensive patients than in the control patients, but the difference was not signigicant statistically (27.2 ±17.5 μIU/ml vs 19.9 ±18.9 μIU/ml, p = 0.1297). The mean insulin concentration of the essential hypertensive patients with diabetes mellitus was twice that of the normotensive patients with diabetes mellitus. In control group, there was a direct relations between insulin level and c-peptide, in both fasting state (R = 0.617, p = 0.0001) and glucose-loaded state (R = 0.531, p = 0.001). But in hypertensive group, there was no relations between insulin level and c-peptide, in both fasting state (R = 0.257, p = 0.2738) and glucose-loaded state (R = 0.307, p = 0.1885).

비행청소년의 성격유형과 부모의 성격유형에 따른 비행의 주요요인에 관한 연구

주영신,양승남 朝鮮大學校 社會科學硏究所 2003 社會科學硏究 Vol.24 No.2

한국 Drosophila아속 10종에 대한 유전적 유연관계

주은영,이양숙,김남우 경산대학교 기초과학연구소 2001 基礎科學 Vol.5 No.2

한국산 Drosophila아속 10종(D. virilis, D. tsigana, D. lacertosa, D. angularis, D. brachyephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai, D. unispina)의 계통관계를 알아보기 위하여 동위효소와 수용성 단백질 분석을 실시하였다. 15종류의 동위효소 분석에서 18개의 loci가 검출되었다. 18개 loci중 Mdh-2, Got, Me, Ao, G6pd, Adh, Fum은 종내 변이가 없었으며, 특히 Ao, Fum은 종간에서도 변이가 없이 monomorpic하였다. 이 동위효소 분석에서 D. lacertosa와 D. nigromaculata가 가장 낮은 유연관계를 보였다. 2DE에 의한 수용성단백질 분석에서는 D. nigromaculata와 D. brachynephros가 가장 낮은 유연관계를 보였으며, D. angularis와 D. brachynephros가 가장 높은 유연관계를 보였다. 이상의 결과를 종합하여 보면 한국산 Drosophila아속 10종은 D. virilis, D. tsigana, D. lacertosa의 group과 D. angularis, D. brachynephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai, D. unispina의 group으로 나눌 수 있으며, D. virilis가 속한 group이 더 primitive하다고 하겠다. 그러나 이 group이 다른 group에 직접적인 ancestor는 아니며 다른 ancestor가 있었을 것으로 생각된다. Phylogenetic relationships among the 10 species of the subgenus Drosophila in Korea, which is D. virilis, D. lacertosa, D. tsigana, D. angularis D. brachnephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai and D. unispina were investigated using 18 loci found from analysis of 15 allozymes and soluble proteins analysis. Mdh-2, Got, Me, Ao, G6pd, Adh and Fum showed monomorphic patterns in each species, and specially Ao and Fum are the same alleles. As for the genetic relationships based on the results from the allozymes analysis, D. curvispina and D. unisina were found to have the closest relationship (0.851), and D. lacertosa and D. kuntzei showed the reverse (0.198). the average genetic similarity of the 10 species by allozyme analysis was 0.458. Soluble proteins from the 10 species of subgenus Drosophila were analyzed by 2DE method. Genetic distance between D. angularis and D. brachynephros was closer (0.168) than any other species. D. curvispina and D. unispina, and D. kuntzei and D. takadai were grouped as related species. The allozyme and soluble protein analysis showed similar. The average genetic distance of the 10 species by 2DE analysis was 0.386, indicating that the subgenus Drosophila can be grouped into two classes; the one class in D. virilis, D. lacertosa, D. tsigana and the other D. angularis, D. brachynephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai and D. unispina.

심인성 다음다갈증 2예

양창국,최병무,한홍무,김영대,주승재 동아대학교의과대학 1992 동아의대학술지 Vol.4 No.1

인지-행동프로그램이 보호관찰청소년의 공격성, 충동성 및 자아존중감에 미치는 효과

주영신,양승남 朝鮮大學校 社會科學硏究所 2005 社會科學硏究 Vol.26 No.1

This study was done for juvenile probation's in order to investigate the effectiveness of Cognitive-Behavioral Program on their aggressiveness, impulsiveness and self-esteem. The subjects were 20, mostly high school students and their average age was 16.75. 10 were appointed into the Experimental group, and 10 in the Control group. The subjects of experimental group received 10 sessions(20hours) of the treatment intensively in two months. The results of the study are as fbllowing: First, the subjects of the experimental group showed a significant decrease in their aggressiveness after the treatment compared with those of the Control group. Second, the subjects of the experimental group showed a significant decrease in their impulsiveness after the treatment compared with those of the control group. Third, there was no significant difference after the treatment on their Self-esteem between the two group subjects. However, those in the experimental group reported that they learned how to cope with their specific problem situations - that is, their indicted experience. And they could feel confidence and a sense If achievement through the Cognitive-Behavioral Program. Based on the results, several suggestions were made for future studies.

마돈나 의상에 나타난 젠더 정체성 : 뮤직비디오 텍스트를 중심으로

김주영,양숙희 服飾文化學會 2002 服飾文化硏究 Vol.10 No.1

The purpose of this research is to understand the gender identity expressed in Madonna music video texts and performances. Madonna has reconstructed the fluid identities through the variations of body, image, costumes, and attitudes. The results are as follows; ① Her punky sexuality is to be seen the flash trash look, kitsch fashion, which deconstructs a good/bad taste, modesty/immodesty, the relations of under/outer wear using bawdy sexuality through her early Virgin tour. ② Her Heterosexuality is to be seen the glamourous look, traditional images of women, which represents the passive feminity of patriarch. ③ Her sadomasochism sexuality is to be seen the bondage look of dominatrix image, which deconstructs sexual taboos; represents sexual power. ④ Her bisexuality is to be seen androgynous look, the 3rd species look using masculinity/feminity signifier, which deconstructs the stereotypes of gender roles. ⑤ Her homosexuality is to be seen the fetish fashion by drag and lesbian, which deconstructs the dichotomy of normality/perversion; opens a possibility of women subjectivity of sexual desires.