http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 장윤경 (검색결과 230 건)
- 무료
- 기관 내 무료
- 유료
-
Pediastrum simplex Meyen 의 분류학적 연구
장윤경,김미란 ( Yoon Kyung Chang,Mi Ran Kim ) 한국하천호수학회 1994 생태와 환경 Vol.27 No.1
In order to reexamine the Pediastrum simplex group in Korea, 11 strains of Pediastrum simplex group from 11 different locations in Korea were isolated and cultured in the laboratory. 3 species 1 variety of Pediastrum simplex group reported in Korea; P. simplex, P, simplex var. duodenarium, P. biwae, P. clathratum were reexamined and classified as P. simplex var. simplex and P. simplex var. pseudoglabrum according to their marginal cell shape and cell wall ornamentation. The existing taxonomic characteristics such as cell number, colony form, presence or absence of cavity were variable within the same culture. The cell wall ornamentation and the marginal cell shape were the most distinctive and decisive characters for the infraspecific classification of P. simplex.
-
배양된 사람 각막내피세포에서 Cyclosporine A로 유도된 노화의 효과
장윤경,정태영,신영주 대한안과학회 2020 대한안과학회지 Vol.61 No.9
Purpose: To investigate the in vitro effect of cyclosporine A (CsA)-induced senescence on human corneal endothelial cells (HCECs). Methods: HCECs were cultured and incubated with 0-100 μM CsA. Senescence-associated β–galactosidase (SA-β-gal) staining was performed. Mitochondrial dehydrogenase activity was assessed using a WST-8 assay kit and mitochondrial membrane potential (ΔΨm) was measured using JC-1 dye. Intracellular and mitochondrial formation of reactive oxygen species (ROS) was measured with 2’,7’-dichlorodihydrofluorescein diacetate and MitoSOX probes. Intracellular and mitochondrial calcium levels were measured using Fluo-4 and Rhod-2, respectively. Protein expression was evaluated by Western blotting. Results: CsA increased the percentage of SA-β-gal-positive cells (p = 0.003) and decreased mitochondrial dehydrogenase activity and ΔΨm in a dose-dependent manner (p = 0.029, p = 0.004). Intracellular and mitochondrial ROS levels increased during incubation with CsA (p = 0.005). CsA at 100 μM increased mitochondrial calcium levels (p = 0.001), whereas intracellular calcium levels decreased at 100 μM CsA (p = 0.029). CsA activated GSK3β and ERK1/2 and reduced ZO-1 expression. Conclusions: CsA induces senescence in HCECs through oxidative stress and via mitochondria-, GSK3β-, and ERK1/2-dependent pathways. Thus, concentrations of CsA should be monitored. 목적: 생체 외에서 배양된 사람 각막내피세포에서 cyclosporine A (CsA)로 인한 노화의 영향에 대해 알아보고자 하였다. 대상과 방법: 사람 각막내피세포를 배양하고 0-100 μM CsA와 incubation을 시행하였다. 노화 연관 베타 갈락토시다제(senescenceassociated β-Galactosidase, SA-β-gal) 염색을 시행하였다. WST-8 분석 키트를 사용하여 미토콘드리아 탈수소효소의 활성을 평가하고, JC-1 dye를 이용하여 미토콘드리아막 전위(ΔΨm)를 측정하였다. 세포 내와 미토콘드라아에서 활성 산소 형성을 측정하였다. 세포 내와 미토콘드리아의 칼슘 수치를 각각 Fluo-4와 Rhod-2를 통해 측정하였다. 단백질 발현은 웨스턴 블롯(western blot)에 의해 평가되었다. 결과: 농도 의존적으로 CsA는 SA-β-gal 양성 세포의 비율을 증가시켰고(p=0.003) 미토콘드리아 탈수소효소의 활성과 ΔΨm을 감소시켰다(p=0.029, p=0.004). 세포 내 및 미토콘드리아의 활성 산소는 CsA와 배양하는 동안 증가하였다(p=0.005). 100 μM의 CsA에서 미토콘드리아 칼슘 수치가 높아진 반면(p=0.001), 세포 내 칼슘 수치는 낮아졌다(p=0.029). CsA는 GSK3β와 ERK1/2를 활성화시키고 ZO-1 발현을 감소시켰다. 결론: 사람 각막내피세포에서 CsA는 산화 스트레스의 유도와 미토콘드리아, GSK3β 및 ERK1/2 경로를 통해 노화를 유도한다. 따라서 고농도의 CsA 사용시 주의하여 사용하여야 한다.
-
장윤경,송태진 대한신경과학회 2022 대한신경과학회지 Vol.40 No.3
Dyslipidemia is a major risk factor for stroke, following hypertension, diabetes, and smoking. Treatment of dyslipidemia is important for the prevention and treatment of various vascular diseases, including stroke, coronary artery disease, and peripheral vascular disease. Statins, ezetimibe, and proprotein convertase subtilisinkexin type 9 (PCSK9) inhibitors have been shown to reduce the risk of stroke and cardiovascular disease in previous studies. When prescribing dyslipidemia drugs for secondary prevention of stroke, the combination, type, and dose of dyslipidemia drugs should be appropriately selected according to the patient's comorbidity and stroke subtype. In the case of statins, highintensity statin administration should be considered, but it is essential to take into account side effects, comorbidities, and individual characteristics of each patient. If the target low density lipoproteincholesterol level is not reached or inappropriate drug dosing due to the side effects of statins, ezetimibe or a PCSK9 inhibitor may be considered. Finally, statin discontinuation due to improper judgment may be detrimental to the patient in the longterm view.
-
-
-
-
장윤경,김진권,우호걸,류동열,오형중,송태진 대한신경과학회 2020 Journal of Clinical Neurology Vol.16 No.1
Background and Purpose Fibroblast growth factor 23 (FGF23) is associated with atherosclerosis via nitric-oxide-associated endothelial dysfunction and calcium-phosphate-related bone mineralization. This study aimed to determine the association of the plasma FGF23 concentration with intracranial cerebral atherosclerosis (ICAS) and extracranial cerebral atherosclerosis (ECAS). Methods We prospectively enrolled 262 first-ever ischemic stroke patients in whom brain magnetic resonance was performed and a blood sample acquired within 24 h after admission. Plasma FGF23 concentrations were measured using an enzyme-linked immunosorbent assay. The presence of ICAS or ECAS was defined as a ≥50% decrease in arterial diameter in magnetic resonance angiography. The burden of cerebral atherosclerosis was calculated by adding the total number of vessels defined as ICAS or ECAS. Results Our study population included 152 (58.0%) males. The mean age was 64.7 years, and the plasma FGF23 concentration was 347.5±549.6 pg/mL (mean±SD). ICAS only, ECAS only, and both ICAS and ECAS were present in 31.2% (n=82), 4.9% (n=13), and 6.8% (n=18) of the subjects, respectively. In multivariate binary and ordinal logistic analyses, after adjusting for sex, age, and variables for which p<0.1 in the univariate analysis, the plasma FGF23 concentration (per 100 pg/mL) was positively correlated with the presence of ICAS [odds ratio (OR)=1.07, 95% CI=1.00–1.15, p=0.039], burden of ICAS (OR=1.09, 95% CI=1.04–1.15, p=0.001), and burden of ECAS (OR=1.06, 95% CI=1.00–1.12, p=0.038), but it was not significantly related to the presence of ECAS (OR=1.05, 95% CI=0.99–1.12, p=0.073). Conclusions The plasma FGF23 may be a potential biomarker for cerebral atherosclerosis, particularly the presence and burden of ICAS in stroke patients.
-
-
-
코리아스칼라
KCI연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
