자가 분해된 곰팡이 Aspergillus nidulans 배양액에서 Superoxide dismutase 분리

강정헌,김종일 서울여자대학교 자연과학연구소 2013 자연과학연구논문집 Vol.25 No.-

Superoxide dismutase, which plays a very important role in protecting organisms from oxygen toxicity, from centrifuged autolyzed cultures of Aspergillus nidulans was prepared to apparent homogeneity by a combination of Q-Sepharose, S-Sepharose and Sephacryl S-100 column chromatography. A. nidulans SOD is a type of Fe-SOD or cambialistic Zn/FeSOD, judging by metal analysis and its insensitivity to azide and a sensitivity to H2O2. The molecular weights of the purified enzyme estimated by gel filtration chromatography are 86 kDa. The SOD seems to be a homodimeric protein with a molecular mass of 35 kDa, which is determined by polyacrylamide gel electrophoresis, per monomer and contained one atom of iron per protein molecule. Maximum SOD activity was observed between pH 5.0 to 7.0.

레이저와 2차원 배열의 광전검출기를 이용한 구조물의 변위측정 시스템의 개발에 관한 연구

강문필,이진이,김민수,김대정,최원하,강기훈,김종수,김훈 한국비파괴검사학회 2002 한국비파괴검사학회지 Vol.22 No.1

일반적으로 구조물은 외부의 정적 및 동적 하중과 외부환경으로 인하여 피로균열과 부식이 발생하며, 이것은 구조물의 변형을 유발하여 결국 파단으로 이어지기 때문에 균열과 부식의 검출 및 평가와 함께 구조물의 변형에 의한 진동, 변위 기울기와 같은 거동을 감시하는 것도 매우 중요하다. 이에 레이저 센서 시스템을 이용하여 이러한 거동을 측정하여 이상 유무를 모니터링할 수 있는 구조물 안전감시 시스템을 개발하였다. 본 시스템은 2차원으로 배열한 광전센서를 이용하여 구조물의 변형에 의해 유발된 광궤적의 변화를 감시하며 또한 데이터를 취득하고 신호처리 할 수 있는 운용 프로그램도 갖추고 있다. 본 연구에서는 개발한 안전 진단 시스템의 필드 적용에 앞서 실험실에서의 몇 가지 실험을 통하여 그 효용성을 검증하였다. A Safety Monitoring System using a laser and 2-D arrayed photo sensors is developed. To monitor of the deformation and small rotation of structure the developed optical system using 2-D photo sensor array was used to detect the variation of optical orbit of laser which was induced by deformation of the structure. Also, an operating program to manage the system and an algorithm for the data acquisition and the database are introduced. In this study, we demonstrated the capabilities of this system by laboratory experiments before applying the system to the field.

유전자 재조합 인간 성장호르몬(rhGH)이 NK 세포의 활성에 미치는 영향

강창랑,김종덕,류훈,정헌택 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

To evaluate the acute effect of recombinant human growth hormone(rhGH) on the activity of natural killer(NK) cell in children, we selected 6 patients of growth hormone deficient and 5 normal control children. In both groups, 0.15 IU/kg of rhGH was administered subcutaneously. The plasma growth hormone level were measured by radioimmunoassay on 0, 2, and 6 hours after administration of rhGH. To determine NK cell activity, peripheral blood mononuclear cell were separated by Ficoll-Hypaque gradient sedimentation. After this, varying concentration of viable effector cells in complete medium were added to duplicate culture of target cells(the human erythroleukemia cell line, K562) and we measured the LDH released from the cytosol into 16 hours culture medium. The average paasma level of growth hormone of growth hormone deficient and normal group were increased to the level of 41.6±23.7 and 96.3±46.5 ng/ml 2 hours after rhGH injection respectively and decreased to the level of 18.510.6 and 42.2± 5.5 ng/ ml 6 hours after rhGH injection respectively. In 16 hours_ culture medium, percent cytotoxicity of NK cell was significantly increased in the blood samples of control groups. It seemed that administration of the rhGH do not made a any effects acutely on NK cell activity in growth hormone deficient group. But in a normal control children, external administration of growth hormone increased percent cytotoxicity of NK cell activity.

의료기기 QI 활동 개선방안에 대한 연구

강훈희,주라형,김종순,김서확,허수진 한국의료QA학회 1998 한국의료질향상학회지 Vol.5 No.2

Background : Medical equipments take a very important role in diagnosis and treatment of disease in modern medicine and effective maintenance of the equipments is a necessory to provide a good healthcare to the public. After developing a new QC program for effective maintenance of medical equipments and practicing it for a year, we report the results of the new program. Methods : The maintenance data of 9 equipments in 8 categories including a CT Scanner were analyzed with regard to the parts responsible for most frequent failure and cause of the failure. After learning the most frequent failure part and cause of the failure, we developed a new QC program that emphasizes preventive maintenance of the most frequent failure part. We compared the number of failure per year and active rate of each equipment before, and after the adoption of the new QC program. Results : The average number of failure per year per equipment was 20.7 before and it decreased by 43% to 11.9 after adoption of the new QC program. The average active rate of the equipments was 92.6% before and it increased by 3.2% to 95.8% after adoption of the new program. Conclusions : The practice of the new QC program appears very useful as it decreased the failure rate and increased the active rate of the equipments.

부비동 내시경수술 후 비음도 변화

동헌종,김선일,권중근,윤영선,강수미,추광철 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

말기신부전 환자에서 혈액투석 전후의 QT간격분산 비교

강대웅,정지용,윤나라,안치용,김종오,신병철,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Background: Some cases of QT interval, corrected QT interval (QTc), QT dispersion (QTd) and corrected QT dispersion (QTcd) have been reported in ESRD, but these results are variable and the influence of a hemodialysis is still controversial, In this study, we investigated the effect of hemodialysis on QT and QTc interval and QT and QTc dispersion in patients with ESRD 15 min before and 15 minute after each hemodialysis. Methods: Sixty-seven patients with ESRD (men 33 & women 34) on three-times stable hemodialysis (>3 months) were randomly enrolled. Fifty control subjects with a similar age and normal renal function were enrolled from this hospital. Routine biochemical studies were measured pre- and post dialysis, at the time of the ECG. Plasma Na+, K+, BUN, creatinine, ionized calcium and phosphate were checked. Twelve-lead electrocardiographs were performed at 10㎜/mv and 50㎜/s using a HewIett-Packard Pagewriter 100, before and after a single hemodialysis session.The QT interval was measured from the onset of the QRS complex to the end of the T wave. If the end of the T wave was not clear in a particular lead then it was excluded from analysis. When U waves were present, the end of the T wave was taken as the nadir between the T and U waves. Each QT interval was corrected for heart rate using Bazett's formula (QTc==QT/√(RR)) (ms). The difference between maximal and minimal QT interval duration was defined as QT dispersion (QTd) in each of the 12 leads. Result: This study demonstrates that QT, QTd, QTcd is higher in hemodialysis patients compared with control subjects, and QT and QTd rise postdialysis to levels comparable to those seen acutely following myocardial infarction, when patients are at greatly increased risk of potentially fatal ventricular arrhythmias, Conclusions: QT interval and QT dispersion, markers of risk for arrhythmias and sudden death, are elevated in hemodialysis patients, and rise postdialysis. QT interval and QT dispersion is an easily obtainable, noninvasive, simple, inexpensive, and widely available method of risk stratification in uremic patients receiving chronic dialysis. Additional studies are needed to clarity whether increased postdialysis QT dispersion results in an increased occurrence of arrhythmias.

감마선과 Methyl Bromide 처리가 사과의 생리화학적 품질에 미치는 영향

강호진,정헌식,조덕조,변명우,최성진,최종욱,권중호 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

감마선(0, 0.5, 1, 2, 3 kGy) 조사와 methyl bromide(MeBr, 26 g/㎏, 4hr) 훈증 및 이들의 처리시기가 사과의 생리화학적 품질특성에 영향을 비교하여, 검역처리를 위한 ◁그림 삽입▷ (원문을 참조하세요) 감마선 조사의 응용성을 검토하였다. 감마선과 MeBr 처리에 따른 사과의 생리화학적 품질평가에서 2kGy 이상의 감마선 조사와 MeBr 훈증은 사과의 일부 생리화학적 품질특성의 변화를 촉진시키는 것으로 나타났다. 감마선과 MeBr의 처리시기별로는 수확 직후보다는 저온(0℃)에서 40일 정도 보관 후 처리한 대체적으로 품질손상이 적은 것으로 나타났다. 따라서 1kGy 이하의 감마선 조사와 지연조사는 사과 과실의 품질을 비교적 양호한 상태로 유지할 수 있어 검역처리 기술로 활용성이 있는 것으로 생각된다. 'Fuji' apples were treated by gamma irradiation (0∼3 kGy) or methyl bromide fumigation at commercial conditions (MeBr, 25 g/㎏, 4 hr) for quarantine purpose at two different treatment timing after harvest and stored under air a 0℃ for 4 months. Associated with the treatment timing of irradiation or fumigation, after 40 days of storage at 0℃ following harvest was more adequate than immediately after harvest in keeping qualities of stored apples. However, more than 2 kGy irradiation and MeBr fumigation were detrimental to physiological and chemical qualities of the fruits. These results show that less than 1 kGy irradiation at the delayed timing has a possibility to be applied as a quarantine procedure without significant changes in the quality of apples.

우울과 관련된 장애를 가진 환자들의 PAI 프로파일

두정훈,강종구,노승호,오상우 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

신장조직 손상의 진행에 있어서 Renin-Angiotensin System 의 역할에 관한 연구

이강욱,송정헌,강민규,황평주,김종학,나기량,서광선,박정규,신영태 대한신장학회 2001 대한신장학회잡지 Vol.20 No.3

콜레스테롤 생합성 저해제 검색법의 개발

임종철,이남훈,김한균,염형렬,이대호,강현석,류형선,명재일,김완,신부안 全南大醫大 2001 전남의대학술지 Vol.37 No.2