Acremonium chrysogenum에 의한 Cephalosporin C 생산 및 모델링

이종일,류화원,박돈희 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

The production of cephalosporin C and its biosynthetic modeling were studied. Cultivation experiments were carried out in an agitated and aerated fermentor using Acremonium chrysogenum. Corn steep liquor was used as a major carbon source. Intracellular and extracellular concentrations of metabolites and enzymes were measured. Based on enzymatic kinetics the biosynthetic modeling of cephalosporin C was developed and used to compare the experimental data with simulation results. In the simulation the effect of dissolved oxygen concentraction on cephalosporin C production was investigated. For the high production yield of cephalosporin C the dissolved oxygen concentration should be kept over 40 % during the cultivation. The biosynthetic modeling can help further to understand the biosynthetic machinery of the microorganism to produce cephalosporin C.

'가족적응력 및 결속력 평가척도 한국어판'의 표준화 예비연구-신뢰도 및 타당도

이종일,성덕규,이민규 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.6

목적: 가족환경은 정신질환의 치료 과정과 경과, 재발. 예후에 중요한 역할을 하고 있는 것으로 생각되고 있어 가족구조의 평가 및 가족치료는 정신병리의 연구와 치료에서 매우 중요하다. 이 예비연구는 가족구조의 적응력과 결속력을 평가하는 가족 적응력 및 결속력 평가척도(Family Adaptability and Cohesion Evaluation Scale, FACES ll)의 한국어 판에 대한 신뢰도와 타당도를 검증하기 위해 실시되었다. 방 법: 총 30문항으로 구성된 FACES ll를 한국어로 번역하여 서울, 부산 지역에서 무작위표집한 정상 가족의 구성원 124명에게 작성토록 하여 척도화 가능한 요인을 추출하고 그 신뢰도를 알아보았다. 또한 변별타당도를 알아보기 위해 환자군은 국립서울정신병원에서 치료중인 정신분열병환자 가족의 구성원 62명을 표집하였고, 대조군은 정상 가족의 구성원 60명을 무작위표집하여 설문조사를 실시하였다. 결과 : 분석 결과는 다음과 같다. 1) '정서적 유대' '수용과 공유, '참석, '타협과 만족'. '책임과 기대'의 5개 하위 척도로 구성된 23개 문항의 '가족 적응력 및 결속력 평가척도'를 얻을 수 있었으며 이들의 내적 일치도를 나타내는 신뢰도(Cronbach's alpha)계수는 각각 .83. .75, .71, .75, 71이었다. 2) 변별타당도에서 '책임과 기대'하위 척도를 제외한4개의 하위 척도에서 대조군의 평균치가 환자군보다 유의하게 높았다. 결 론: 한국어촌 가족 적응력 및 결속력 평가척도는 신뢰도와 타당도를 고려할 매 가족구조의 중요한 요소인 적응력과 결속력을 평가하는 유용한 척도로 생각된다. 그러나 요인분석과정중 7문항이 제외된 점과 FACE II의 요인 분석과 다른 점에 대해서는 향후 개선된 연구가 필요한 것으로 생각된다. 0bjectives : Family environment is important in treatment course, relapse, and prognosis of psychiatric illnesses. Thus the evaluation of family structure and the family therapy is important in the investigation and treatment of psychopathology. This preliminary study was designed to attempt to verify the reliability and validity of a Korean version of Family Adaptability and Cohesion Evaluation Scale ll(K-FACES ll) which evaluate the adaptability and cohesion of family structures. Method : The translated FACES ll which constructed a questionnaire composed off total of 30 items was applied to 124 member from families with no psychiatric patient in Seoul and Pusan by random sampling. Then the authors extracted the factors which were able to compose the scale and evaluated the concurrent reliability. Also for the discriminative validity test, the authors selected 62 members, who have been no psychiatric history, of families with schizophrenic patient diagnosed with the criteria of DSM-IV who halve been treated in Seoul National Mental Hospital and 60 members of families without schizophrenic patient in Seoul and Pusan for control. Result : The results were as fo11ows : The authors extracted 5 subscales of emotional bonding, acceptability and sharing, participation, negotiation and satisfaction, responsibility and expectation of 'Family Adaptability and Cohesion Evaluation Scale' which constructed a questionnaire composed of a total of 23 items. Cronbach's alpha value of each subscale which show its internal consistency was .83, .75, .71, .75, .71 respectively. In the discriminative validity test all subscales except'responsibility and expectation'subscale significantly disaiminated between family group with schizophrenic patient and control group. Conclusion: These findings confined the high reliability and validity of preliminary form of K-FACES ll and suggest the possibility that K-FACES ll could be a useful measure to evaluate the adaptability and cohesion of family structures. But we think that further study should be needed considering some limitations that 7 items were excluded and extracted victors could not be matched with FACES in factor analysis

생물공정 모니터링을 위한 흐름주입분석 기술 개발

김준홍,이종일 전남대학교 촉매연구소 2001 觸媒硏究 論文集 Vol.22 No.-

Since the first description of flow injection analysis system (FIA) by Ruzicka and Hansen the FIA system has been developed for automation of selective assays in many fields. Recently. it has been applied on the analysis of chemical and biochemical components and also on the bioprocess monitoring extensively. In this paper, we describe the on-line monitoring technique for the concentration of carbohydrates such as glucose and starch, which are often used as major carbon source in many biological processes. Lactate is important product in a few fermentation processes. The concentrations of lactate during the cultivation can be also online monitored by a FIA system. In this study the availability of lactate-FIA is investigated for a biohydrogen production process.

헬리코박터 파이로리 균 진단용 ^13C-요소 캅셀의 개발

용철순,김용일,김지만,강성훈,권기철,이종달,김종국,사홍기,최한곤 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.1

The purpose of this study was to develop a new ^13C-urea- containing capsule for diagnosis of H. pylori. The urea-containing capsules were prepared with various diluents such as polyethylene glycol (PEG), microcrystalline cellulose, sodium lauryl sulfate and citric acid. The dissolution test, ^13C-urea breath test and stability test were then performed on the capsules. Microcrystalline cellulose and sodium lauryl sulfate retarded the initial dissolution rates of urea. However, PEG increased the initial dissolution rates of urea. Furthermore, two formulae composed of PEG, [^13C-urea/PEG (38/1.9 mg/cap)] and [^13C-urea/PEG/citric acid (38/1.9/1.9 mg/cap)] had the maximum DOB value, about 16 at 20 mim, while the formula composed of only 38 mg ^13C-urea had the maximum DOB value at 30 min. The results indicated that PEG improved the sensitivity of ^13C-urea in the human volunteers. The capsule [^13C-urea/PEG (38/1.9 mg/cap)] was stable for at least six months in 25 and 37℃. Thus, a PEG-containing capsule, [^13C-urea/PEG (38/1.9 mg/cap)] would be a more economical, sensitive and stable preparation for diagnosis of H. pylori.

정신분열병 환자에서 사회인구학적 특징과 종교에 따른 정신병 치료방법에 대한 선호도

이창민,이종일,신경희,이민규,김태현 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 종교가 정신분열병 치료에 도움을 주기도 하지만 정신병 치료방법에 대한 잘못된 개념을 심어주어 환자의 치료순응도에 나쁜 영향을 미치기도 한다. 그러므로 종교에 따른 정신병 치료방법에 대한 선호도를 조사하여 종교가 정신분열병 치료에 미치는 영향을 알아보고자 이 연구를 시행하였다. 방 법 : DSM-Ⅳ에 의해 진단 받았으며 GAF score가 41이상은 정신분열병 환자 102명을 대상으로 하였다. Allport와 Ross의 내재적-외향적 종교성향척도를 이용하여 종교성향을 설문 조사하였으며 정신병 치료방법(초자연적 치료, 원시적 약물치료, 심리적 치료, 종교적 치료, 생물학적 치료)에 대한 선호도를 설문 조사하였다. 수집된 자료에서 사회인구학적 특성과 종교성향 그리고 정신병 치료방법의 관계를 비교 분석하였다. 결 과 : 1) 정신분열병 환자는 생물학적 치료와 심리적 치료에 가장 높은 선호도를 보였으며 다음으로 종교적 치료와 원시적 약물치료에 높은 선호도를 보였다. 2) 기독교를 믿는 환자군은 종교적 치료에 유의한 선호도를 보인다. 3) 내재적 성향의 환자군이 반종교적 성향의 환자군에 비해 심리적 치료에 유의하게 높은 선호도를 보였다. 그리고 친종교적 성향의 환자는 초자연적 치료, 심리적 치료 그리고 종교적 치료에서 유의하게 높은 선호도를 보였다. 결 론 : 전반적으로 종교성향과 정신병 치료방법은 유의한 상관관계를 보이지 않았다. 그러나 종교성향은 특정한 교리나 사상을 반영하기보다는 개인의 성격을 반영하는 경향이 있으므로 종교과 정신병 치료방법이 전혀 무관하다고 할 수 없다. 환자들은 종교적 치료와 원시적 약물치료에 상당한 선호도를 보이고 있으며 특히, 기독교를 믿는 환자군은 종교적 치료에 유의한 선호도를 보인다. 그러므로 환자와 가족에게 정신분열병에 대한 정신교육을 지속적으로 시행해야 한다고 여겨진다. Objectives : Religion may be beneficial to the treatment of schizophrenia, but religion frequently have bad influence on drug compliance because religion give false concept of the treatment of psychosis. We investigated the influence of religion on the treatment of schizophrenia by assessing the preference for the treatment of psychosis to religion. Methods : 102 patients of schizophrenia who were diagnosed by DSM-Ⅳ and above 41 scores of GAF score were selected. We assessed religious orientation with the Intrinsic and Extrinsic Religious Orientation Scale and assessed the preference for the treatment method of psychosis(supernatural treatment, primitive medicinal treatment, psychological treatment, religious treatment, biological treatment). The relationship among the variables of demographic data, religious orientation, the treatment method of psychosis were analyzed. Results : 1) Schizophrenic patient showed first preference for biological treatment and psychological treatment and they showed second preference for religious treatment and primitive medicinal treatment. 2) Protestant showed preference for religious treatment. 3) Patient group who have intrinsic religious orientation showed more preference for psychological treatment than patient group who have antireligious orientation. Conclusions : Generally the relation between religious orientation and the treatment method of psychosis was not significant. But it couldn't be said that religion and the treatment method of psychosis was not related because religious orientation was not related with religious ideology but was related with personality. Schizophrenic patient showed more preference for religious treatment and primitive medicinal treatment and specifically, protestant showed preference for religious treatment. Therefore, we suggest that it is necessary to give persistent education about schizophrenia to patients and their family.

헬리코박터 파이로리 균 진단용 ^13C-요소 캅셀의 개발

용철순,김용일,김지만,강성훈,권기철,이종달,김종국,사홍기,최한곤 영남대학교 약품개발연구소 2002 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

The purpose of this study was to develop a new ^13C-urea-containing capsule for diagnosis of H.pylori. The urea-containing capsules were prepared with various diluents such as polyethylene glycol (PEG), microcrystaline cellulose, sodium lauryl sulfate and citric acid. The dissolution test, ^13C-urea breath test and stability test were then performed on the capsules. Microcrystalline cellulose and sodium lauryl sulfate retarded the initial dissolution rates of urea. However, PEG increased the initial dissolution rates of urea. Furthermore, two formulae composed of PEG,[^13C-urea/PEG (38/1.9 mg/cap)] and [^13C-urea/PEG (38/1.9/1.9 mg/cap)] had the maximum DOB value. about 16 at 20 min, while the formula composed of only 38 mg ^13C-urea had the maximum DOB value at 30 min. The results indicated that PEG improved the sensitivity of ^13C-urea in the human volunteers. The capsule [^13C-urea/PEG (38/1.9 mg/cap)] was stable for at least six months in 25 and 37℃. Thus, a PEG-containing capsule, [^13C-urea/PEG (38/1.9 mg/cap)] would be a more economical, sensitive and stable perparation for diagnosis of H. pylori.

폴록사머를 이용한 디클로페낙 고형 좌제의 개발

용철순,오유경,김정애,김용일,박상만,양준호,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

To develop a poloxamer-based solid suppository with poloxamer mixtures, the melting points of various formulations composed of P 124 and P 188 were investigated. To investigate the effect of poloxamer to the dissolution and dissolution mechanism of diclofenac sodium from the suppository the dissolution of diclofenac sodium delivered by the poloxamer-based suppository was performed. Furthermore, to investigate the mucoadhesive property of the poloxamer-based solid suppository, the identification test in the rectum was carried out after its rectal administration in rats. The poloxamer mixtures composed of P 124 and P 188 were homogeneous. Very small amounts of P 188 affected the melting points of poloxamer mixtures. In particular, the poloxamer mixture [P 124/P 188 (97/3%)] with the melting point of about 32℃ was a solid form at room temperature and instantly melted at physiological temperature. Furthermore, very small amounts of P 188 in the poloxamer-based suppository hardly affected the dissolution rates of diclofenac sodium from the suppository. Dissolution mechanism analysis showed the dissolution of diclofenac sodium was proportional to the time. At 4 h after administration, the blue color of poloxamer-based suppository [diclofenac sodium/poloxamer mixture (2.5/97.5%)] with the P 124/P 188 ratio of (97/3%) and blue lake in the rectum was faded. However, the position of suppository in the rectum did not significantly change with time. Thus, it retained in the rectum for at least 4 h. Our results indicated that the poloxamer-based solid suppository with P 124 and P 188 would be a candidate of rectal dosage from for diclofenac sodium.

프레탈정 (실로스타졸 100mg)에 대한 실로스탄정 (한국유나이티드 제약)의 생물학적 동등성

용철순,이경희,최진석,박병주,정세현,김용일,박상만,유봉규,이종달,최한곤 한국병원약사회 2003 병원약사회지 Vol.20 No.2

Bioequivalence of one cilostazol tablets, the Pletaal^(R)(Korea Otsuka Pharmaceutical Co., Ltd.) and Cilostan^(R)(Korea united Pharmaceutical Co., Ltd.), was evaluated according to the guideline of KFDA. Sixteen normal male volunteers(age 20~30 years old)were divided into two groups and a randomized 2×2 cross-over study was employed. After one tablet containing 100㎎ of cilostazol were orally administered, blood was taken at predetermined time intervals and the concentration of cilostazol in plasma was determined with an HPLC method using UV detector. The pharmacokinetic parameters(C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameter. The results showed that the differences in C_(max), T_(max) and AUC_(t) between one tablet were 16.08%, 18.88% and 17.57%, respectively. The powers (1-β) for C_(max), T_(max) and AUC_(t) were 85.03%, 83.92% and 80.12%, respectively. Detectable differences(Δ) and confidence intervals were all less than 20%, and confidence interval of all the parameters were also less than 20% at the significance level(α) of 0.05. All of these parameters met the criteria of KFDA for bioequivalence, indicating that Cilostan^(R) tablet is bioequivalent to Pletaal^(R) tablet.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.