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박돈희,정귀택,Gwang-Yeon Lee,Jin-Myoung Cha 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.1
In order to develop a method for the removal of hydrogen sulfide via a biological process, two different packing materials were tested to assess their capabilities as biofilter bed materials under variable conditions of two parameters: inlet gas concentration and inlet gas flow rate. We detected a maximal elimination capacity (critical loading rate) of 515.1 (410.5) g-H2S/m3·hr, and 415.5 (80.0) g-H2S/m3·hr, respectively, when polypropylene fibrils and volcanic stone were employed as supporting materials. The results of this study show that the application of polypropylene fibrils might be a favorable choice as a packing material in biofilter for the biological removal of hydrogen sulfide
朴暾熙 연세대학교 대학원 1977 원우론집 Vol.5 No.1
Abstract The investigation is concerned with magnesia concentration by froth flotation from domestic dolomite. Conditioned pulverized dolomite was foaled with pine oil, as a frothing agent, oleic acid, as a collector and K2CO3 as a pH regulator, in a specially made fioating machine. Pulp density leas carefully controlled at an oplimum condition.
박돈희,Il Jung,Kyungmoon Park 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.4
The growth conditions of Pantoea agglomerans, a phosphate solubilizing organism, were studied in our laboratory to determine the optimal conditions. Pantoea agglomerans showed the highest growth rate at 30°C, pH 7.0 and 2 vvm, after 50 h cultivation. A certain relationship between pH and phosphate concentration was evident when the glucose concentration in the medium was changed. Increasing glucose concentration increased the pH buffer action of the broth. At glucose concentrations higher than the optimum concentration of 0.2 M, the cell growth was retarded. P. agglomerans consumed glucose as a substrate to produce organic acids which caused the pH decrease in the culture medium. The phosphate concentration in the medium was increased by the presence of the organic acids, which solubilized insoluble phosphates such as hydroxyapatite.
박돈희,정귀택 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6
Hairy root cultures have demonstrated great promise in terms of their biosynthetic capability toward the production of secondary metabolites, but continue to constitute a major challenge with regard to large-scale cultures. In order to assess the possibility of conducting mass production of biomass, and the extraction of useful metabolites from Panax ginseng. P. ginseng hairy roots, transformed by Rhizobium rhizogenes KCTC 2744, were used in bioreactors of different types and sizes. The most effective mass production of hairy roots was achieved in several differently sized air bubble bioreactors compared to all other bioreactor types. Hairy root growth was enhanced by aeration, and the production increased with increasing aeration rate in a 1 L bioreactor culture. It was determined that the hairy root growth rate could be substantially enhanced by increases in the aeration rate upto 0.5 vvm, but at aeration rates above 0.5 vvm, only slight promotions in growth rates were observed. In 20 L air bubble bioreactors, with a variety of inoculum sizes, the hairy roots exhibited the most robust growth rates with an inoculum size of 0.1% (w/v), within the range 0.1 to 0.7% (w/v). The specific growth rates of the hairy roots decreased with increases in the inoculum size.