Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

한국산 배의 Polyphenol 화합물군이 쥐의 면역기능에 미치는 영향

최희진,한호석,박정혜,배종호,우희섭,안봉전,배만종,김현구,최청 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.4

This study was conducted to investigate immunofunctional activity of the polyphenol fractions isolated from Korean pear. In the experimental of Rosette forming cell, the results showed that all the polyphenol fractions enhance the cell count compared with the control group. Especially polyphenol fraction II and III showed highly significant effect on Rosette forming cell, and allergy inhibition. After antigen challenge, histamine content of blood of the polyphenol groups was lowered to near the normal group.

단기간의 금연과 일회성 유산소 운동이 흡연자의 심혈관 반응에 미치는 영향

송준경(Jun Kyung·choi),최현민(Hyun Min Choi),김종경(Jong Kyung Kim),노호성(Ho Sung Nho) 한국사회체육학회 2012 한국사회체육학회지 Vol.0 No.48

The purpose of this study was to investigate cardiovascular responses to a bout of aerobic exercise and acute smoking cessation in smoker. nine collegiate male smoker who have been smoking(duration of smoking: 7.5 ± 0.9yrs; number of cigarettes per day: 15.5 ± 1.5) participated in this study. All subjects performed PEI to stimulate Group IV metaboreceptor at immediately after smoking, 24hours after smoking cessation and smoking after a bout of aerobic exercise. they were measured SBP, DBP, HR, SV, CO, TVC using by finometer(FMS co, Netherland). As a result, compared to immediately after smoking, SBP, DBP, and MAP were significantly decreased 24-hours after smoking cessation at rest and PEI(p<0.05). In addition, smoking after a bout of aerobic exercise caused by post-exercise hypotension indicated that SBP was significantly decreased compared to immediately after smoking(p<0.05). also, the changes in DBP and MAP at rest and handgrip exercise were significantly smaller compared to immediately after smoking(p<0.05). Thus, the results showed that smoking cessation during 24-hours and a bout of aerobic exercise relieve excess blood pressure response and enhanced arterial baroreflex in collegiate male smoker. It is concluded that caused exercise pressor reflex by stimulated Group IV may be effective to improve excess blood pressure responses.

최근 10년간 원광의대병원에서 시행한 소아 심장혈관 수술의 임상적 고찰

윤향석,정수미,최두영,오광수,오연균,김종덕,양현웅,이삼윤,김형곤,최종범,최순호,노병석 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

We reviewed 10 years experiences of the clinical aspects and early postoperative results in 544 pediatric patients(age less than 16 years old). Among them. 529 cases had the congenital heart diseases, and 15 had the acquired diseases. Open heart surgery was performed in 413 patients. 115 cases were treated with non-open heart surgery. 16 with interventional catheterization. Annual increase of the cardiac patients was not significant in recent 10 years, except slight increase in 1994. The mean age of the study patients had been evidently changed to younger year by year. Total mortality rate of the open heart surgery was 6.4%. The cyanotic congenital heart diseases were high in early postoperative mortality by 25.7%, whereas 4% in acyanotic group. The age group less than one month was most highest in surgical mortality(66.7%). There were no deaths in patients with patent ductus arteriosus(103 cases), atrial septal defect(83 cases) or pulmonary stenosis(17 cases). Among 220 patients with ventricular septal defects(VSD). 10(4.5%) were died early postoperatively. In cyanotic group, the patients with pulmonary atresia with ventricular septal defects(PAVSD), transposition of the great arteries(TGA), interrupted aortic arch(IAA), complex cardiac anomalies with isomerism seemed to be most susceptible to an early death.

백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

陸上競技 記錄의 豫測과 性差

崔鐘浩,金基學 大邱敎育大學校 1992 論文集 Vol.27 No.-

본 연구는 육상경기의 중장거리경기 및 도약경기를 중심으로 과거의 기록을 분석하여 미래의 기록을 예측하고 남녀간의 기록차를 검토하기 위해서 행해졌으며, 그 대상이 된 기록은 1972sys부터 1990년 까지의 전국체육대회 고등부와 일반부의 100m,200m, 400m, 400m Relay, 800m,1500m 높이뛰기 및 멀리뛰기의 우승기록이다 경기기록의 예측은 1972년부터 1989년까지의 18년간의 기록을 기초로 해서 1990년, 1991년,1992년 및 2000년의 기록을 추정했으며, 1990년의 실제기록과 추정기록을 비교하고 전 기간을 통한 기록변화과정에서 성차가 검토되었다. 육상경기 기록의 추정과 성차에 대한 결론은 아래와 같다. 1. 회기 방정식의 회귀계수가 트랙종목에서는 -자로, 필드종목에서는 +로 되어 있으며 기록향상을 계속해서 이루어질 것으로 예측되지만, 단거리 일수록 기록단축의 범위가 좁고 중거리에서는 그 범위가 클 것으로 예상된다. 2. 여고부의 100m 및 남자 일반부의 100m와 400m를 포함한 대부분의 종목을 제외한 전종목에 걸쳐 비교적 추정의 精度가 높게 나타나고 여고부의 멀리뛰기는 가장 높았다.(R=0.9422) 3. 남자 고등부 멀리뛰기의 추정기록이 2000년에는 남자 일반부를 능가하게 되는 것으로 나타나고 있는데 이것은 과대평가 되어진 결과로 해석된다. 4. 전 종목에 걸쳐 남자 일반부의 기록 향상이 가장 저조할 것으로 보이는데 이것은 현재까지의 기록이 우리나라 선수로서는 기록의 최고치에 해당되는 한계선에 도달해 있는 것으로 사료된다. 5. 실측기록과 추정기록을 비교해 본 결과 아주 적은 차로서 추정기록이 실측기록보다 더 좋은 것으로 나타나고 있지만 비교적 추정의 精度가 높으므로 연도로서 장래의 기록을 거의 정확하게 추정할 수 있을 것으로 예상된다. 6. 단거리에서의 성차는 약 80-90% 정도이지만 2000년의 추정기록에서는 남자의 약 94%정도 근접해갈 것으로 보아 그 차는 약6%정도가 될 것이다. 7. 중거리에서 고등부는 현재 88%에서 2000년에는 94%로 근접해 가지만, 일반부에서는 83%에서 87%까지 접근해 가서 그 차는 약 13%정도가 될 것이다. 8.높이뛰기에서는 여고부의 기록향상이 남고부에 비해 저조하지만, 일반부는 2000년에 약 94%가 되어 기록 향상이 두드러질 것이다. 9. 멀리뛰기에서는 여고부의 기록향상이 남고부에 비해 저조하지만, 일반부는 현재 23%에서 14%로 접근해 갈 것이다. 10.경기기록 향상의 잠재성은 남자보다 여자가 더 클 것으로 기대된다.

投形運動 指導를 위한 生體力學的 硏究

崔種浩,朴政和 대구교육대학교 1994 論文集 Vol.29 No.-

The Biomechanical characteristics related to the throwing in later Children are investigated between the unskilled and the skilled group. For the study, 12 unskilled boys and 10 skilled boys aged 11 were selected at random. 44 variables are investigated in total; 14 item in Anthropometry, 7 item in Motor abiligy, and 23 item in Biomechanics. The form of throwing a rubber ball with a weight of 150g and a diameter of 7cm is filmed at the rate of 100 fps by means of two 16mm high speed cinecamera for the DLT. Each variable was satistically analyzed and between the unskilled and the skilled group. The conclusions are summarized as follows ; Standing height, lower limb length and shoulder width are lager in the skilled group than in the unskilled group, which implies that the growth of body length and width has an effect on the development of the ball throwing skill. The skilled are better in she motor ability except flexibility than the unskilled. In general, the ball throwing ability is affected by the other motor ability and is not developed sufficiently in later children. The motor patterns of the ball throwing, with she exception of the velocities of ball, hand and wirst at release and relaese angle are mastering at 11 years of age. The closer segmental end, the larger maximum segmental velocity is observed. The difference of maximum velocities of connected segments in uppers limb due to development of muscular system. The segmental movement of the best thrower is whiplike, in sequence from torso to upper limb end.

競技力 向上을 위한 Training原理에 關한 硏究 : 陸上運動을 中心으로 On Track and Field Events

崔鍾浩 大邱敎育大學校 1993 論文集 Vol.28 No.-