Up‑regulation of miR‑27 extenuates lipopolysaccharide‑induced injury in H9c2 cells via modulating ICAM1 expression

Jing‑Fang Xiang,Jian‑Chun Yu,Jian‑You Zhu 한국유전학회 2019 Genes & Genomics Vol.41 No.12

Background MiR-27 has been found to present an overt myocardial expression during cardiogenesis. However, whether miR-27 involves in myocarditis development and the possible molecular mechanism remain unknown. The purpose of this study was to investigate the biological characteristic of miR-27 in LPS-damaged H9c2 cells. Methods H9c2 cells were treated with lipopolysaccharide (LPS, 10 μg/ml) for 12 h to form cell injury. MiR-27 mimic and inhibitor were used to up-regulate or down-regulate miR-27 expression. MTT assay and flow cytometry analysis were conducted to test cell viability and apoptosis. The relative RNA expression level of miR-27 and intercellular adhesion molecule 1 (ICAM1) was determined by qRT-PCR. Luciferase reporter gene assay was utilized to confirm the interaction between miR-27 and ICAM1. Western blot was used to determine the protein expression levels. Results We observed that LPS treatment significantly decreased the level of miR-27 in H9c2 cells. Moreover, LPS exposure suppressed cell viability, promoted cell apoptosis and increased the relative expression of p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα. Up-regulation of miR-27 increased cell proliferation and reduced cell apoptosis, while down-regulation of miR-27 suppressed cell growth and promoted cell apoptosis. ICAM1 was predicted and verified as a target of miR-27, and the expression of ICAM1 is negatively regulated by miR-27. The relative expression of p-NF-κB p65/NF-κB p65 and p-IκBα/ IκBα was dramatically decreased by miR-27 mimic and increased by miR-27 inhibitor. Conclusion Our study illustrated that up-regulation of miR-27 exhibits a protective effect on LPS-damaged H9c2 cells, which may be achieved by regulating ICAM1 and NF-κB signaling.

Tissue-specificity of RNA editing in plant: analysis of transcripts from three tobacco (Nicotiana tabacum) varieties

Fang Jing,Jiang Xiao-Han,Wang Teng-Fei,Zhang Xiu-Jun,Zhang Ai-Di 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4

RNA editing is a significant post-transcriptional molecular process of modifying the primary transcripts by editosome. In plants, it remains unknown whether and to what extent RNA editing contributes to tissue-specific regulation from a global perspective. To obtain an overview of RNA editing events in model plant tobacco (Nicotiana tabacum), we implemented a bioinformatics analysis of DNA-Seq and RNA-Seq data from roots and leaves of three tobacco varieties (TN90, Basma and K326). The results showed that hundreds of RNA editing sites were detected to be located in the protein-coding region of plastid/mitochondria for all three varieties. Among these sites, some of them were detected in leaves but not or reduced in roots. Interestingly, most of the disappeared editing sites in roots were located in plastid transcripts encoding subunits of NADH dehydrogenase. The average editing efficiencies in roots were reduced significantly compared with leaves across three varieties in both organelles. In addition, we found that the reduction of RNA editing efficiency in mitochondria is mild compared with plastid. Expression analysis further showed that an extraordinarily high percentage of RNA editing factors were down-regulated in roots, particularly for PPR, MORF proteins, indicating that the distinct editing patterns between roots and leaves might result from the differential regulation of RNA editing factors expression. This study provides references and insights into understanding the function of plant RNA editing in tissue-specific regulation mediated by editosomes.

Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius

Jing Fang,Xi Peng 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.4

The aim of this work was to investigate developmental changesin cell proliferation and apoptosis in normal duck bursa ofFabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 ofembryogenesis (E24 and E27) along with days 20, 70, and 200 ofpostnatal development (P20, P70, and P200). Results showedthat the percentage of G0/G1 bursa cells significantly increasedbetween E24 and P200 while the percentage of cells in the Sphase or G2 + M phase as well as the proliferating indexobviously decreased during the same period. Proliferation cellnuclear antigen was detected in lymphocyte and interfollicularepithelium. The proliferative lymphocyte density tended todecrease from E24 to P200. Apoptotic bodies in macrophages,free apoptotic bodies, or nuclei with condensed chromatin inlymphocytes in follicles were identified by transferase-mediateddUTP nick-end labeling. Both flow cytometry and microscopicanalysis reveal that the proportion of apoptotic cells andapoptotic lymphocyte density increased from E24 to P20, fell onP70, then rose again on P200. Our foundings demonstrate thatcell proliferation decreases and apoptosis increases with age. These changes may account for duck bursa development and involution.

Digital immediate denture: A clinical report

Fang, Jing-Huan,An, Xueyin,Jeong, Seung-Mi,Choi, Byung-Ho Elsevier 2018 The Journal of prosthetic dentistry Vol.119 No.5

<P><B>Abstract</B></P> <P>Fabricating an immediate complete denture in the conventional manner may be complicated and difficult. The purpose of this article was to describe a fully digital workflow used to fabricate an immediate complete denture before removing the teeth and without the use of conventional impressions or stone casts. The digital data for the workflow were acquired using an intraoral scanner and were then used to design the denture base and teeth after deleting any remaining tooth images from the virtual 3-dimensional image of the jaw. The resulting data were exported to a milling machine for denture fabrication.</P>

MiR-188-5p regulates the proliferation and differentiation of goat skeletal muscle satellite cells by targeting calcium/calmodulin dependent protein kinase II beta

Jing Jing,Sihuan Zhang,Jinbo Wei,Yuhang Yang,Qi Zheng,Cuiyun Zhu,Shuang Li,Hongguo Cao,Fugui Fang,Yong Liu,Ying-hui Ling Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.12

Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.

Electro-hydraulic coupling jet printing technology based on Weissenberg effect under pulse voltage

Jing Wang,Xintao Wu,Wei Wang,Bing Xu,Yuanjie Fang,Jing Tang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6

In this paper, electro-hydraulic coupling jet printing technology based on the Weissenberg effect is put forward for the first time, and the pulse voltage is used to realize the on-demand printing. The experimental results show that the Weissenberg effect can inhibit the solution reflux and make the solution converge into a cone tip at the nozzle, which is more conducive to position printing precisely. By exploring the influence principles of process parameters on printing, it is found that droplet diameter is positively correlated with rotation speed and negatively correlated with voltage frequency. The printing frequency is negatively correlated with the rotation speed and positively correlated with the voltage frequency. However, the effect of voltage amplitude and duty ratio on the printing effect is not obvious in a certain range. It is found that the rotation speed of needle core within the range of 1800 r/min-7500 r/min can print out the ideal lattice structure, which proves the feasibility of this system to realize on-demand positioning printing. Finally, by using the printing equipment, the sacrificial layer structure of PDMS microfluidic chip was successfully made and the function of the microfluidic chip was realized, which further verified the practicability of the technical scheme proposed in this paper.

Effects of contact shape on ballistic phonon transport in semiconductor nanowires

Jing-Jing Yao,Wei-Qing Huang,Gui-Fang Huang,Yuan Chen,Ling-Ling Wang,Wangyu Hu,Anlian Pan 한국물리학회 2012 Current Applied Physics Vol.12 No.2

We study the effects of contact shape on ballistic phonon transport in semiconductor nanowires at low temperatures using an approximative scalar model of continuum elasticity. Five different contacts connected to two semiconductor nanowires with different transverse widths are discussed. Numerical results show that the contact shape acts as an ‘acoustic impedance adaptor’, playing a crucial role on the ballistic phonon transmission and thermal conductance. The phonon coupling in the contacts with certain length facilitates ballistic phonon transmission compared to the abrupt interface, in which the phonon scattering is the strongest. It is found that the more the contact is abrupt, the smaller the thermal conductance is. The catenoidal contact rather than the abrupt interface is also the competitive candidate to obtain bigger thermal conductance. These results indicate that choosing an appropriate contact shape is one of the most critical factors to accurately measure the thermal conductance with a very high precision and reliability in different temperature ranges at low temperatures.

TAp73 and ΔNp73 Have Opposing Roles in 5-aza-2'-Deoxycytidine-Induced Apoptosis in Breast Cancer Cells

Jing Lai,Fang Yang,Wenwen Zhang,Yanru Wang,Jing Xu,Wei Song,Guichun Huang,Jun Gu,Xiaoxiang Guan 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.8

The p73 gene contains an extrinsic P1 promoter and an intrinsic P2 promoter, controlling the transcription of the pro-apoptotic TAp73 isoform and the anti-apoptotic ΔΝp73 isoform, respectively. The DNA methylation status of both promoters act equally in the epigenetic transcriptional regulation of their relevant isoforms. The aim of this study was to analyze the different effects of these p73 isoforms in 5-aza-2'-deoxycytidine (5-aza-dC)-induced apoptosis in breast cancer cells. We investigated the effects of the DNA demethylation agent, 5-aza-dC, on the T-47D breast cancer cell line, and evaluated the methylation status of the p73 promoters and expression of TAp73 and ΔNp73. Furthermore, we assessed the expression of p53 and p73 isoforms in 5-aza-dC-treated T-47D cells and p53 knockout cells. 5-aza-dC induced significant anti-tumor effects in T-47D cells, including inhibition of cell viability, G1 phase arrest and apoptosis. This was associated with p73 promoter demethylation and a concomitant increase in TAp73 mRNA and protein expression. In contrast, the methylation status of promoter P2 was not associated with ΔNp73 mRNA or protein levels. Furthermore, demethylation of P2 failed to inhibit the expression of ΔNp73 with 5-aza-dC in the p53 knockdown cell model. Our study suggests that demethylation of the P1 and P2 promoters has opposite effects on the expression of p73 isoforms, namely up-regulation of TAp73 and down-regulation of ΔΝp73. We also demonstrate that p53 likely contributes to 5-aza-dC-induced ΔNp73 transcriptional inactivation in breast cancer cells.

Heterologous expression of two Physcomitrella patens group 3 late embryogenesis abundant protein (LEA3) genes confers salinity tolerance in arabidopsis

Jing Du,Lei Wang,Xiaochen Zhang,Xuan Xiao,Fang Wang,Pingliang Lin,Fang Bao,Yong Hu,Yi-Kun He 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

Salinity stress is a major limiting factor in agriculture and adversely affecting the whole plant. As a halophyte, the moss Physcomitrella patens, has been suggested to be an ideal model plant to study salinity tolerance and adaption. Two abiotic stress-responsive Group 3 Late Embryogenesis Abundant protein genes had been identified in P. patens and named as PpLEA3-1 and PpLEA3-2, respectively. Functions of these two genes were analyzed by heterologous expressions in Arabidopsis, driven either by their native P. patens promoters or by the 35S CaMV constitutive promoter. Phenotype analysis revealed that pLEA3::LEA3, pLEA3::LEA3::GFP and 35S::LEA3::GFP transgenic lines had stronger salinity resistance than that in the wild type and empty-vector control. Further analysis showed that the contents of proline and soluble sugar were increased and the malondialdehyde (MDA) were repressed in these transgenic plants after exposure to salinity stress. Our observations indicate that these two Group 3 PpLEA genes played a role in the adaption to salinity stress.

A hemoglobin-based oxygen-carrying biomimetic nanosystem for enhanced chemo-phototherapy and hypoxia alleviation of hepatocellular carcinoma

Jing-Qing Le,Fang Yang,Xun-Huan Song,Ke-Ke Feng,Ling-Wu Tong,Meng-Die Yin,Wen-Zhong Zhang,Ying-Qi Lin,Hui Wu,Jing-Wei Shao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

Tumor microenvironment is characterized by low pH, high reactive oxygen species and hypoxia, whichprovides a suitable environment for cancer growth. The hypoxia not only elevates tumor angiogenesisand metastasis, but also is responsible for the development of treatment resistance, which graduallybecomes a significant impediment for cancer therapy. Therefore, we developed a biomimetic nanosystemcontaining hemoglobin extracted from red blood cells, chemotherapy drug sorafenib, sensitizer ursolicacid and photosensitizer indocyanine green for enhanced chemo-photo combination therapy of hepatocellularcarcinoma, which could not only enhance the chemotherapy effect of sorafenib bowing to thesensitizing effect of ursolic acid, but also achieved synergetic phototherapy in virtue of indocyaninegreen. Besides, the nanoparticles could effectively delivery exogenous oxygen to tumor site and amelioratethe tumor hypoxic environment with the assistance of hemoglobin. The dual-sensitization drugdelivery system was expected to effectively reduce the resistance of traditional treatment methodsagainst tumor hypoxia, providing a novel prospect for the synergistic hepatocellular carcinomatreatment.