Toluene, Xylene, Trichloroethylene 투여가 흰쥐의 시상하부와 뇌하수체의 GnRH, GnRH Receptor, Pit-1 유전자 발현에 미치는 영향

기노석,이종태,강대성,김대환,이채관,이헌,전진호,김정호,이채언 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2

The workplace exposure of chemicals has steadily increased, therefore the concern for subsequent effect on reproductive outcome has been an important issue in occupational medicine. In previous studies, higher rates of spontaneous abortion, reduced fertility and menstrual disorder among women, and an impairement of sperm quantity and quality among men have been associated with a wide variety of chemical agents. This study was conducted to evaluate the effects of toluene, xylene and trichloroethylene(TCE) injection on the mRNA levels of GnRH, GnRH receptor and Pit-1 genes in male rats hypothalamus and pituitary and the effects on the plasma levels of FSH, LH, prolactin and testosterone. Sprague-Dawley male rats were divided into five groups of five each according to concentration of toluene, xylene and TCE. The rats were injected subcutaneously to 0, 50, 100, 200, 400 mg/kg body weight/day of toluene, xylene and TCE, respectively for 6 days. Rat brains were excised and hypothalamus and pituitary were separated. Reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay(RPA) were used to evaluate the GnRH, GnRH receptor and Pit-1 mRNA levels. Plasma concentrations of FSH, LH, prolactin and testosterone were assayed by radioimmunoassay(RIA). The results were as follows; 1. GnRH, GnRH receptor and Pit-1 mRNA levels in toluene and xylene injected groups, and GnRH receptor mRNA levels in TCE injected group were lowered dose-dependently. Especially, GnRH receptor and Pit-1 mRNA levels in 200mg/kg of toluene injected group, and GnRH, GnRH receptor and Pit-1 mRNA levels in 400mg/kg of toluene injected group were significantly lower than control group (p<0.05). GnRH receptor and Pit-1 mRNA levels in 400mg/kg of xylene injected group, and GnRH receptor mRNA levels in 400mg/kg of TCE injected group were significantly lower than control group(p<0.05). 2. The plasma levels of prolactin and testosterone in 400mg/kg of toluene injected group, and LH in 100, 200 and 400mg/kg of xylene injected group, and testosterone in 400 mg/kg of TCE injected group were significantly lower than control group(p<0.05). In conclusion, we speculated that toluene and xylene affected reproductive system secondarily through hypothalamus-pituitary axis, and TCE affected directly through steroidogenesis. And we recommended that further study for assessment of the reproductive toxicity of mixed organic solvent exposures should be conducted.

원통 연삭에서 가공 능률 향상을 위한 연삭 저항의 측정에 관한 연구

김원일,이진,이윤경,왕덕현,이상복,이관철,문원길 경남대학교 공업기술연구소 2000 硏究論文集 Vol.18 No.-

본 연구에서는 원통 연삭 가공에서 가공 능률 향상을 위한 연삭 저항을 측정하고자 연삭 가공 중에 발생하는 연삭 저항의 추이를 확인하였고 다음과 같은 결과를 얻었다. 숫돌과 공작물간의 연삭 강성은 공작 속도의 증가에 따라 지지계의 강성이 높은 경우 증가하였고 절삭 깊이와 연삭 숫돌의 속도에는 연삭 강성이 감소하는 경향을 보였다. 또한 총합 강성은 공작 속도, 절삭 깊이 및 연삭 숫돌의 속도 증가에 따라 지지계의 강성이 높을 수록 증가하였다. 연삭기의 강성 변형과 가공 능률에 영향을 주는 값으로 연삭 강성비는 지지계의 강성이 높을 수록 감소하였다. In this study, the measurement of grinding resistance to improve grinding efficiency in cylindrical grinding was studied and the following conclusions could be obtained. Relation grinding wheel and workpiece, grinding stiffness increases according to increase of work speed for high machine stiffness and decreases depth of cut and grinding wheel speed. Also, resultant stiffness increases high machine stiffness according to increase work speed, depth of cut and grinding wheel speed. The values of the effect stiffness deflection in grinding machine, grinding stiffness ratio decrease according to high machine stiffness.

진위천 유역 수질 특성 분석

박병기 ( Byeong Ky Park ),김진혁 ( Jin Yuk Kim ),이동준 ( Dong Jun Lee ),신경용 ( Kyung Yong Shin ),박민지 ( Min Ji Park ),문정숙 ( Jeong Suk Moon ),이영준 ( Young Joon Lee ),이재관 ( Jae Kwan Lee ) 한국물환경학회(구 한국수질보전학회) 2014 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2014 No.-

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

cDNA array 방법을 이용한 망간에 노출된 흰쥐 뇌기저핵의 유전자발현 분석

이채관,노성민,문덕환,,김정호,손병철,김대환,이창희,김휘동,김정원,김종은,안진홍,이채언 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study investigated the gene expression profile in basal ganglia of manganese-exposed rats based on cDNA array analysis. Methods: For cDNA array, 25 male Sprague-Dawley rats (250±25 g) were intraperitoneally injected with 25 ㎎/㎏ B. W./day of MnCl2 (0.3 ㎖) for 10 days. For dose-related gene expression analysis, rats were intraperitoneally injected with 0.2, 1.0, and 5.0 ㎎/㎏ B. W/day of MnCl2 for 10 days. Control rats were injected with an equal volume of saline. RNA samples were extracted from brain tissue and reverse-transcribed in the presence of [α^(32)P]-dATP. Membrane sets of the Atlas Rat 1.2 array Ⅱ and Toxicology array 1.2 kit (Clontech, Palo Alto, CA) were hybridized with cDNA probe sets. Northern blot hybridization method was employed to assess the dose-related gene expression. Results: Fifty-two genes showed significant changes in expression of more than two-fold. Twenty-eight were up-regulated and 24 were down-regulated in the manganese-exposed group compared to the control. Among the 52 genes, 28 genes including nuclear factor I-X1 (NF1-X1), neuroligin 2 and 3, mitochondrial stress-70 protein (MTHSP70), neurodegeneration-associated protein 1 (Neurodap 1), multidrug resistance protein (MDR), and endoplasmic reticulum stress protein 72 (ERP72), were reported for the first time related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia. According to the dose-related gene expression analyses, MTHSP70, Neurodap 1 and ERP72 genes were up-regulated compared to the control even in the group exposed to low manganese dose ( 0.2 ㎎/㎏ B.W./day). Conclusions: Twenty-eight genes detected for the first time in this study were closely related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia and further study of these genes can give some more useful information about the manganese metabolism.

공기소총 사격시의 생체변화 분석

엄기진,이철규,박장평,이광재,문병용,홍관이,노성규,오수일,박기동 江原大學校附設體育科學硏究所 1985 江原大學校附設體育科學硏究所論文集 Vol.- No.10

This study conducted for analyze the process of physiological variation during shooting, and produce the basic data for training prescription to the performance improvement in Air Rifle Shooting, 9 Elite Rifle shooters )College 3, High School 3, Middle School 3) in GangWeon province were tested the physiological parameter which are Electrocardiograph(E.C.G), Heart-rate(H.R), Respiratory pattern, and Trigger time by polygraph system 8 channel from Sept. 1st to Sept. 10th, 1985. Analyzed experimental results are as follows; 1. Firing at T-P wave in E.C.G, 77.68bpm in H.R)16.68% by resulting H.R), the deep size of inspiration-expiration was low, and 8.34 sec of trigger interval were appeared when the shooter acquired high score. 2. The most effective factor among physiological parameters for excellent shooting score is the inspiratory volume with decresc breathing pattern. 3. Shooting performances correlated with stability and static-dynamic balance, but flexibility effected on negative correlation.

통사처리과정에서 한국어-영어 이중언어자의 뇌 활성화

이승복,연은경,이다미,정관진 한국뇌학회 2002 한국뇌학회지 Vol.2 No.2

영어 유창성이 높은 한국어-영어 이중언어 후기 습득자들이 관계절 문장의 문법성 판단검사로 통사처리과제를 수행하는 동안 기능적 자기공명 영상을 측정하였다. 10명의 영어유창성이 높은 참여자들이 두 언어로 제시되는 문법성 판단검사를 수행하였다. 두 언어의 통사처리수행에서 활성화되는 뇌 영역을 통제과제, 응시과제와의 감산으로 산출하고, 두 언어 조건에서의 활성화 강도를 비교하였다. 그 결과, 두 언어의 처리 영역은 전반적으로 비슷하였으나, 한국어(Ll) 문장의 처리에 비해 영어(L2) 문장을 처리할 때 전두엽의 양쪽 반구에서 더 많은 활성화를 보였다. 전두엽의 양쪽 반구에서 더 많은 활성화를 보였다. 한국어 처리의 경우에는 오른쪽 전두엽의 활성화가 보이지 않는 반면, 측두엽의 활성화가 관찰되었다. 이러한 활성화 양상에 관해 이중언어와 통사처리의 특성이라는 면에서 논의하였다. To examine whether the bilinguals' two languages are represented in distinct or overlapping areas of the brain, we applied functional magnetic resonance imaging (fMRI). Ten proficient Korean-English (K-E) bilinguals participated in the experiment. We conducted a grammaticality judgment task in which subjects were asked to judge grammaticality of various kinds of relative clause constructions. The results showed that while the locations of activation were similar for both languages, the intensity of activation in these areas were much greater in I.2 English than I.1 Korean. It was also found that there was greater activation in left frontal regions during I.2 English processing than during I.1 Korean processing. As for the right frontal regions, the activation was observed only in I.2 English processing. However, Middle temporal gyrus was found to be activated during I.1 processing, not I.2 processing. We discussed the results as to the bilingualism and syntactic processing.

충주시 농촌지역 주민들에 대한 보건교육 평가

권복규,양준호,송진범,이건세,이원진,장성훈 건국대학교 의과학연구소 1996 건국의과학학술지 Vol.5 No.-

The aim of this study was to evaluate the effect of health education program in Choongju rural area which had conducted by Public Health Doctor during winter in 1994 and 1995. We used two step sampling method for a household survey. First, six were selected from 12 Myuns, which were stratified by the level of health education activity. Second, we applied convenient sample method. Of the 307 respondents, most of them knew Health-subcenter's existence in their region. However, only 149 persons(45%) visited it during last year. They visited Health-subcenter because it was easy to travel and convenient. Some of them thought that Health-subcenter had very poorly equipped and supplied. About 32∼34% of respondents participated the health education program for hypertension and diabetes mellitus which was done last winter, and 59∼70% of participants of the program answered that it was very, moderately helpful. 82∼83% of participants recognized complications of hypertension and diabetes mellitus, and 18∼20% of them did not know that medications of hypertension and diabetes mellitus should be continued one's whole life. The respondents want to receive health educations for cancer, arthritis, viral hepatitis and AIDS in orders. Many of rural residents utilize Health-subcenters because health clinics in Choongju rural area are very few.

체육교사의 자기수업장학이 교수행동에 미치는 영향

이병익,김관현,김진표,김기환,김윤태 龍仁大學校 2001 용인대학교 논문집 Vol.19 No.-

This study was to analyze the effect of psical teacher's self-directed super- vision on teaching behavior. The prpose of this study is to identify the effect of pysical teacher's self director supervision of their teaching trough other teacher's teaching and all of physical education methods. Therefore research models and hypothesis were established on previous research and theories. Background variable was physical teacher's personal factor and Independent variable was physical teacher's sect-directed supervision. Dependent variable was teaching behavior of physical teacher. The data were collected by using simple random sampling of 360 physical teacher's in Seoul , Kyung-gi-Do, and In-chun. To survey the data question paper was used. T-test and one-way ANOVA were used to test hypothesis. Multiple-Regression Analysis was used to know effect of physical teacher's self-directed supervision on teaching behavior. Each hypothesis was tested at the significance level of 0.5 The conclusions were drawn as the followings First. influence of self- directed supervision by physical teacher's personal factor was following. According to sender there was no difference in teaching behavior. There was a significant influence in understanding students according to school's situation(girls, boys, and co-ed). There was a significant influence in class managing, understanding students, class preparing, teaching method according to class reflection. Second. influence of teaching behavior by physical teacher's personal factor was following influence. The gender had influence on dynamism and creativity. School situation(boys, girls, co-ed) had influence on individuality and creativity. Class reflection had influence on dynamism, creativity, obviousness and individuality. Third. effect of physical teacher's self-directed supervision on teaching behavior was followings. In dynamism, it had influence on teaching method, class managing, understanding students, sequentially. But not on preparation. In Individuality, it had influence on preparation, understanding students. Class managing sequentially. But not on teaching method. In obviousness. It had influence on class managing and Preparation. sequentially. but not on understanding students. and teaching method. In creativity, it had influence on understanding students, preparation, class manacling sequentially, but not on teaching method.

특별활동 부서 배정업무 자동화 시스템 구축

이진관,김귀복,박기홍 군산대학교 정보통신기술연구소 2000 情報通信技術硏究論文集 Vol.4 No.-

This thesis tries to rule out the problems of the existing method in which teachers assigns students the club activity department respectively in manual manner. The proposed system assigns club activity department automatically on the web according to the students' application. And thus it reduces both time and cost spent in organizing, arranging and managing which characteristic provides teachers with a turning point in teaching- learning fields.