Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

[P8-249] Capsanthin, the Main Compounds in Red Paprika Fruit (Capsicum annuum L.), Prevents H<SUB>2</SUB>O<SUB>2</SUB>-Induced Inhibition of Gap-Junction Intercellular Communication

Ji?Sun Kim,Jiyun Ahn,Sun-hye Lim,Suna Kim,Tae-Youl Ha 한국식품영양과학회 2010 한국식품영양과학회 학술대회발표집 Vol.2010 No.10

학교보건교육에서의 매체활용실태 및 영향요인 분석

박은옥,안지영,정혜선,김영임,박정영 韓國學校保健學會 1999 韓國學校保健學會誌 Vol.12 No.2

The objectives of this study are to explain the use of media materials in school health education with other related factors in elementary, middle, and high schools in Korea. The data were collected by questionnaires from June to September in 1988. The number of subjects were 294 school nurses. The PC-SAS program was used for statistical analysis such as percent distribution, chi-squared test, spearman correlation test, and logistic regression. The use of media materials in health education has become extremely common. Unfortunately, much of the early materials were of poor production quality, reflected low levels of interest, and generally did little to enhance health education programming. A recent trend in media materials is a move away from the fact filled production to a more affective, process-oriented approach. There is an obvious need for health educators to use high-quality, polished productions in order to counteract the same levels of quality used by commercial agencies that often promote "unhealthy" lifestyles. Health educators need to be aware of the advantages and disadvantages of the various forms of media. Selecting media materials should be based on more than cost, availability, and personal preference. Selection should be based on the goal of achieving behavioral objectives formulated before the review process begins. The decision to use no media materials rather than something of dubious quality will usually be the right decision. Poor-quality, outdated, or boring materials will usually have a detrimental effect on the presentation. Media materials should be viewed as vehicles to enhance learning, not products that will stand in isolation. Process of materials is an essential part of the educational process. The major results were as follows:1. The elementary schools used the materials more frequently. But the production rate of media materials was not enough. The budget was too small for a wide use of media materials in school health education. These findings suggest that all schools have to increase the budget of health education programs. 2. Computers offer an incredibly diverse set of possibilities for use in health education, ranging from complicated statistical analysis to elementary-school-level health education games. But the use rate of this material was not high. The development of related software is essential. 3. In this study, the most effective materials were films in elementary school and videotapes in middle and high school. Film tends to be a more emotive medium than videotape. The difficulties of media selection involved the small amount of extant educational materials. Media selection is a multifaceted process and should be based on a combination of sound principles. 4. The review of material use following student levels showed that the more the contents were various, the more the use rate was high. 5. Health education videotapes and overhead projectors proved the most plentiful and widest media tools. The information depicted was more likely to be current. As a means to display both text and graphic information, this instructional medium has proven to be both effective and enduring. 6. An analysis of how effective the quality of school nurse and school use of media materials shows a result that is not complete)p=0.1113). But, the budget of health education is a significant variable. The increase of the budget therefore is essential to effective use of media materials. From these results it is recommended that various media materials be developed and be wide used.

β-Lactam 유도체의 N(1)-C(4) 위치에 한 개의 탄소를 도입한 입체 선택적 고리확장에 관한 연구

하진렬,오선주,김지아,신동수,안철진 昌原大學校 基礎科學硏究所 2003 基礎科學硏究所論文集 Vol.15 No.-

The base induced ring opening of β -1actam derivatives with LDA gave γ-lactam derivatives stereoselectively. The γ -1actam derivatives were formed stereoselectively depending on β-3 substituent of B-lactam derivatives.

교사가 인식하는 특성화 고등학교 교육의 문제점

이용환,금지헌,안선영 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.4

이 연구의 목적은 특성화 고등학교에 근무하는 교사가 특성화 고등학교에 근무하면서 인식하는 문제점을 파악하고,특성화 고둥학교가 나아가야할 방향을 제시하는데 있다. 이 연구는 교사가 인식하는 문제점을 파악하기 위해 델파이 방법으로 연구되었다. 델파이 패널은 특성화 고등학교로 신설 및 전환된 지 5년 이상 된 학교의 특성화 또는 실과 부장 교사 18명으로 구성되었다. 3차의 델파이 조사가 이루어 졌다. 1차 델파이 질문지는 개방형으로 교사가 인식하는 특성화 고등학교의 문제점을 자유롭게 기술하도록 하였다. 2차 댈파이 질문지는 1차 델파이 조사를 통해 얻은 문제점 54개에 대해 전문계 고등학교의 입장과 특성화 고등학교의 입장 두 가지 측면에서 문제의 심각성을 체크하도록 하였고 추가적인 의견도 들었다. 3차 텔파이 조사는 2차 델파이 조사 결과 얻은 33개의 문제점에 대해 사분위수를 제시하고 심각성을 체크하도록 하였다. 이 때 사분 위수를 벗어나게 되면 그 이유를 적고,추가적인 의견도 받았다. 그 결과 29개의 문제점이 도출되었으며 그 중 심각성이 높은 13개의 문제점은 학생,교사,교육과정,인식 둥에 관한 것은 아래와 같다. 학생과 관련한 문제점: ① 학생들의 대부분이 진학을 희망한다. ② 전반적인 학생의 가정환경 열악하다. ③ 우수한 학생을 확보하기 어렵다. 교사와 관련한 문제점: ① 교사의 행정업무가 과다하다. ② 우수한 특성화 분야 교원을 확보하기 어렵다. ③ 전공교과 교사의 수업시수가 과다하다. 교육과정과 관련한 문제점: ① 학생의 다양성을 충족할 수 있는 특별교육과정 운영을 위한 예산이 부족하다 ② 특성화 프로그램 벤치마킹 대상이 부족하다. 인식과 관련한 문제점: ① 직업교육에 대한 사회 전반적인 인식이 부족하다. ② 특성화 교육에 대한 학부모의 인식이 부족하다. ③ 특성화 교육에 대한 중학교 교사의 인식이 부족하다 기타 문제점: ① 지속적인 지원이 미흡하다. ② 기초기능 함양의 전문 프로그램 제공처가 부족하다. The purpose of this study was to identify the major problems that teachers perceived in implementing specialized vocational high school education, Specialized vocational high schools are promoted as the main national policy to improve vocational education and strongly supported by the Ministry of Education and Human Resources Development. The Delphi technique for obtaining group consensus was employed to achieve the objective of this study. A panel was composed of 18 head teachers as expert participants selected purposively from 22 specialized vocational high schools which were established over 5 years after transferring as a specialized system. Each panel participant received questionnaires to fill-up through e-mail and responded to it. Three sets of questionnaires were prepared by the researchers. The first questionnaire was an open style to express freely each one's opinion on problems related to specialized vocational education. The second and the third questionnaires composed of questions on scale using the Likert type. Several problems were identified, Problems related to specialized vocational high school students were as follows: ① Most of the students want to go to college/university instead of entering the workplace, ② Generally, they are from a socioeconomically disadvantaged background. ③ It is difficult to attract talented students, which has been a chronic problem for vocational high schools, Problems related to teachers were as follows: ① There are excessive clerical work for teachers ② It is difficult to recruit qualified and competent teachers for the specialized areas ③ Teachers are over-burdened with teaching too many hours of vocational classes, Problems related to the curriculum were as follows: ① There are few programs to benchmark specialized vocational education programs and ② There are no systematic assistance to improve basic academic skills of the students, Problems related to recognition were the following: ① Specialized vocational education carries an unfavorable image, ② There is a lack of parents' recognition of the specialized vocational education and ③ Middle school teachers do not have sufficient information or hold unfavorable perception of specialized vocational schools. Other problems identified include ① There are inadequate financial resources to operate the specialized education programs particularly in coping with student's diversified needs and ② There is a lack of continuous governmental support for the specialized vocational education programs.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Advanced Atomic Layer Deposition: Ultrathin metal film growth using discrete feeding ALD and electric potential assisted ALD

Ji Won Han,Hyun Soo Jin,Yoon Jeong Kim,Ji Sun Heo,Woo-Hee Kim,Ji-Hoon Ahn,Jeong Hwan Kim,Tae Joo Park 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.5

Recovery of Solid Fertilizers from human urine through Alkaline dehydration and carbonation

Ji sun Bang,Lulit Habte,Ahn Ji-Whan 한국에너지학회 2023 한국에너지공학회 학술발표회 Vol.2023 No.10

Analysis of the Novel VNTR Polymorphisms of MUC8 Gene

Ji Sun Lee,Ja Young Kim,Eun Kyung Ahn,Sang Yeop Lee,Yun Hee Jeong,Se Ra Lee,Sun Hee Leem 한국유전학회 2009 Genes & Genomics Vol.31 No.3

Analysis of mucin genes has identified the presence of several features that may represent important functional domains in mucin glycoproteins. In the central region of each mucin, there are a variable number of tandem repeats (VNTR; minisatellites). However, their genomic levels are unclear because of complex genomic properties. We report here the distribution of VNTR and polymorphic analysis of MUC8. We searched for VNTR of MUC8 using the Tandem Repeat Finder program and found nine VNTR motif. Six (MUC8-MS1~MS6) among the nine VNTRs were evaluated in this study. Each VNTR in MUC8 region was analyzed in genomic DNA obtained from 200 unrelated individuals and multi-generational families. All VNTRs (MUC8-MS1, -MS2, -MS3, -MS4, -MS5 and -MS6) were genotyped as polymorphic. The degree of polymorphism within the MUC8-MS5 showed the highest heterozygosity (h=0.786) in the MUC8 region. In order to perform a segregation analysis of the VNTRs in MUC8, we analyzed genomic DNA obtained from two generations of five families and from three generations of two families. Six of the polymorphic VNTRs were transmitted through meiosis following a Mendelian inheritance, which suggests that polymorphic VNTRs could be useful markers for paternity mapping and DNA fingerprinting.

Advanced Atomic Layer Deposition: Ultrathin metal film growth using discrete feeding method and electric potential assisted-atomic layer deposition

Ji Won Han(한지원),Hyun Soo Jin(진현수),Yoon Jeong Kim(김윤정),Ji Sun Heo(허지선),Woo-Hee Kim(김우희),Ji-Hoon Ahn(안지훈),Jeong Hwan Kim(김정환),Tae Joo Park(박태주) 한국표면공학회 2022 한국표면공학회 학술발표회 초록집 Vol.2022 No.11