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Web3D의 정보전달 방법에 관한 연구 : Web3D를 이용한 전자상거래에서 정보전달방법을 중심으로
박세라,이동윤,이진영 동서대학교부설연구센터 2001 연구센터논문집 Vol.4 No.1
As the development of the computing technology and the expansion of networking, the way of transmitting information has been changed from two dimension to three dimension. Specially, it has been changes rapidly on the internet. In two dimensional environment uses simply get texts and 2D images, but now we can get information dynamically and really through three dimensional environment. The paper shows the definition and features of Web3D representing 3D technology on the web. We also discuss about E-Commerce on Web3D and actually test the feature of Web3D.
Retinoic Acid 가 Bromodeoyuridine 표지 Hep G2 간암 세포주의 세포 주기 역동성에 미치는 효과
안득수,김대곤,안중기,장동석,이수택,김이엽,이세라 대한내과학회 1993 대한내과학회지 Vol.45 No.5
Objectives: Measuring the total cellular DNA and BrdU content simultaneously by using the monoclonal antibody to BrdU and propidium iodide (PI) has been possible. So it has been advanced that the making a correct diagnosis and the predicting a prognosis in cancer as well as the evaluation of the responses of anti-cancer therapy, We intended to exam new anticancer chemotherapeutic agent, retinoic acid (RA) which has been reported to show antiproliferative effect and also differentiation-inducing effect on tumor cells, for the treatment of the hepatocarcinoma prevailed in our country. This study was designed to probe beneficial effects of RA for preventing tumor recurrence and secondary metastasis through the application of it to recently wide spreaded chemoembolization therapy in heaptomas. Methods: A semi-logarithmic plot of the proliferation was made in Hep G2 cells, and the changes of the proliferative activities from adding RA to the medium were evaluated in according to various concentrations of it. After staining the Hep G2 cells with fluoroscence conjugated anti-BrdU and PI, the tumor cell kinetics was analyzed and the effects of RA on the changes of the kinetics were also evaluated in the univariate and bivariate distribution of flowcytometric measurement of DNA content/BrdU incorporation. Results: Hep G2 cells stained with FITC conjugated anti-BrdU and with propidium iodide (PI), were anal- yzed in the distribution of bivariate BrdU/DNA content or univariate DNA content to investigate the tumor kinetics. Data from this expreiments showed that actual doubling time (Td) is 51 hrs, potential doubling tiem (Tpot) is 29 hrs, the mean DNA synthesis time (Ts) is 9. 3 hrs, the labelling index is 27.6%. From the evaluation of the effects of RA, known to enhance the differentiation and inhibit the growth of tumor cells on Hep G2 cells, RA inhibited the proliferation of Hep G2 cells significantly and the inhibition lasted for 10 days. To understand the growth inhibition of RA on Hep G2 cells in terms of tumor cell kinetics, bivariate BrdU/DNA content distributions were analyzed. Early stage of culture (day 1) showed increase in S phase percentage of cells and decrease in Go/G1 phase. But late stage of culture (day 4) show decrease in S phase percentage of cell to minimum and increase in Go/G1 phase at either 1 μM or 0.1 μM concentration of RA as compared with those of control. The influences on cell cycle and the antiproliferative effect were more pronounced at 1 μM than at 0.1 μM concentration. Conclusion: These results demonstrated that RA inhibits the proliferation of Hep G2 cells and the anti-proliferative effect is suggested to be driven from arresting cell cycle progression from Go/G1 to S-phase in tumor cell kinetics.
Expression and function of recombinant anti-colorectal cancer mAb CO17-1A in SfSWT4 insect cells
Se-Ra Park,Kyung Jin Lee,Jeong-Hwan Lee,David Hedin,Thera Mulvania,Seung Ho Lee,Kisung Ko 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Colorectal cancer is the third most commonly diagnosed cancer in the world, nearly all patients diagnosed with this cancer die from it. Antibodies are glycoprotein molecules, which can efficiently recognize and eliminate specific pathogenic and disease antigens. Antibody researches for the last several decades have demonstrated the potential of therapeutic antibodies to fight cancer. Monoclonal antibody (mAb) CO17-1A recognizes the tumor-associated antigen GA733-2, a cell surface glycoprotein highly expressed in colorectal carcinoma cell, which is applicable for preventing and curing colorectal cancer. We have currently established baculovirus insect cell expression system to produce anti-colorectal cancer mAb CO17-1A. In this study, mAb CO17-1A was expressed in the transgenic insect cell line SWT4, which has humanized glycosylation processing pathway. Immunoblot confirmed that mAb CO17-1A properly expressed in SWT4. mAb CO17-1A was purified using protein G affinity column. In addition, Maldi-TOF verified that the mAb fused to KDEL, ER retention signal had high mannose type of glycan structure whereas the mAb without KDEL had partially humanized glycan structure. These results suggest that the insect cell expression system with the SWT4 possibly can be used as a useful alternative way to produce full-size mAb with humanized glycan structures for cancer immunotherapy.
Lee, Song-Yi,Lee, Yong-Gyu,Byeon, Se-Eun,Han, So-Ryu,Choi, Sun-Shim,Kim, Ae-Ra,Lee, Jae-Hwi,Lee, Sang-Jin,Hong, Sung-Youl,Cho, Jae-Youl 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.11
Sparassis crispa (SC) is an edible mushroom that harbours ${\beta}$-glucans reported to possess immunostimulatory and anticancer properties. The role of SC in regulating the functional activation of macrophages is yet to be fully elucidated. The objective of this study was to investigate the molecular mechanism underlying the immune-stimulatory function of Sparassis crispa soluble ${\beta}$-glucan (Sc-SG) on macrophages. According to this study, Sc-SG was able to stimulate nitric oxide (NO) production as well as enhance the expression of inducible NO synthase (iNOS) from macrophage-like RAW264.7 cells. NO production was strongly suppressed by mitogen-activated protein kinase (MAPK) inhibitors such as U0126, extracellular signalregulated kinase, SB203580, a p38 inhibitor, and SP600125, a c-Jun N-terminal kinase inhibitor. Thus, indicating that Sc-SG-induced NO release is possibly mediated by MAPK. Sc-SG induced phosphorylation of extracellular signal-regulated kinase, p38, and JNK in a timedependent manner. Moreover, Sc-SG triggered the phosphorylation and translocation of c-Jun and c-Fos, components of the transcription factor AP-1, activated by MAPK. The results of this study suggest that MAPK may be a major signaling enzyme that regulates the Sc-SG-mediated NO production in macrophages.