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        제약업종 부산물 및 화장품 제조업 폐수처리오니 처리토양에 대한 유기화합물 및 Bioassay 분석 평가

        임동규,이상범,이승환,남재작,나영은,권장식,권순익,소규호 한국환경농학회 2004 한국환경농학회지 Vol.23 No.4

        본 연구는 퇴비원료로 지정된 제약업종 부산물(공정오니) 및 화장품 제조업 폐수처리오니를 퇴비로서 활용 가능성을 판단하기 위하여 기존의 일반성분이나 중금속 성분 분석 이외 유기성 화합물과 기타 여러 생물검정법 등을 활용하여 시용한 오니에 의한 토양 내 비료의 피해를 밝혀내고 이들의 평가방법을 확립하고자 포장시험을 수행하였다. 오니의 처리에 따른 HEM의 함량은 제약업종 오니2(PS2)와 화장품 오니(CS)처리가 각각 0.51, 1.10 mg/kg로 가장 높았고, PAHs의 함량은 제약업종 오니2(PS2) 처리에서 3,406.8 μg/kg로 가장 높았다. 토양에 서식하는 미소동물의 밀도변화는 생육 중기(7월 23일) 및 수확기(10월 1일) 모두 제약오니2 및 돈분 처리구에서 가장 높았으며, 기타 처리구는 차이가 없이 아주 낮았다. 오니의 처리에 따른 세균 및 사상균의 균수는 제약업종 오니2처리에서 각각 736, 909 cfu/g로 가장 많았고, 화장품 오니도 각각 440, 236 cfu/g으로 다른 처리에 비해 많은 경향을 보였다. 제약오니 및 화장품오니 처리시 우점세균은 무비, 돈분처리에 비해 일정한 경향이 없었으나 3요소 처리보다 다양한 세균이 검출되었다. 오니의 처리에 따른 우점사상균은 무처리에 비해 제약업종 오니3와 화장품 오니처리에서 형태적 특징이 다른 콜로니가 검출되었으나 다른 처리는 비슷한 경향으로 종류 간에 큰 차이는 보이지 않았다. Microcosm test를 통하여 오니 처리 후 3개월이 경과한 토양의 오염정도를 평가한 결과는 제약업종 오니3과 화장품 오니처리는 약간 영향을 받아서 80~90% 생존하였으나, 제약업종 오니1처리는 처리 2주(14일) 이후에는 급격히 생존율이 떨어져서 4주 이후에는 10%만 생존하였다. 그러나 6개월이 경과한 토양에서는 지렁이의 생존율은 제약업종 오니1처리만 약간 영향을 받은 것으로 보였으며 다른 처리들은 전혀 영향을 받지 않았던 것으로 조사되었다. 유기성 오니의 퇴비원료로 활용은 비료관리법의 비료공정 규격 중 퇴비의 비고란에 "퇴비의 원료로 사용 가능한 물질과 사용 불가능한 물질'(별표1)에서 "희비의 원료 중 사전 분석검토 후 사용 가능한 원료에 대한 지정요령'에는 유기물과 중금속(8성분) 함량(건물증)과 제조공정 등을 검토하여 지정하고 있으나, 지정된 원료가 과연 회비원료로 적합한지 잘 알 수 없으며 또한 앞으로 현재의 퇴비원료 규정을 변경할 필요가 있을 경우를 대비하여 희비원료의 적합성 여부를 판별할 수 있는 방법의 개발이 필요하다. 따라서 퇴비원료로 이미 지정('02. 12. 31)된 제약오니 및 화장품 오니를 과량으로 토양에 시용한 후 유해 유기화합물, 미소동물, 미생물 및 생물학적(지렁이) 유해성 검정방법의 도입 가능성을 평가하기 위하여 고추를 재배한 포장에서 비료의 피해시험을 실시한 결과 유해 유기화합물과 생물학적(지렁이) 유해성 검정방법은 앞으로 연구를 통해서 보완할 경우 상당히 활용 가능성이 있는 좋은 평가방법인 것으로 생각된다. This study was conducted to assessment organic compound and bioassay (density of inhabited animal, fluctuation of predominant fungi, and survival ratio of earthworm) for finding damage on red pepper by heavily amount application of sludges in soil, which was treated with 3 pharmaceutical byproducts and a cosmetic industry wastewater sludge as raw materials of compost, and for establishing estimation method. HEM contents in the soil treated with pharmaceutical byproducts sludge2 (PS2) and cosmetic sludge (CS) were 0.51, 1.10 mg/kg respectively. PAHs content of PS2 treatment in the soil was 3406.8 ug/kg on July 8. In abundance of soil faunas, the pharmaceutical byproducts sludge2 treatment was the most highest. The next was decreased in the order of pig manure (PM) and the cosmetic sludge treatment. However the other pharmaceutical sludge treatments were remarkably reduced populations of soil inhabited animals. In upland soil treated with organic sludges, the numbers of bacteria and fungi of the pharmaceutical sludge treatment were 736, 909 cfu/g and those of the cosmetic sludge treatment were 440, 236 cfu/g, respectively. The pharmaceutical sludge treatments and the cosmetic sludge treatment in identification of predominant bacteria were not any tendency to compare with non fertilizer and pig manure treatments, but they had diverse bacteria than NPK treatment. In microcosm tests, the survival of the tiger earthworm in five soil samples was hardly affected against the soil of PSI (20%) after three months treated in the upland. But after six months, survival of PSI was 80%. At present, raw material of compost was authorized by contents of organic matter, heavy metal (8 elements), and product processing according to "The specified gist on possible materials of using after analysis and investigation among raw materials of compost", however, for preparing to change regulation of raw material of compost and for considering to possibility of application, this study was conducted to investigate toxic organic compound and bioassay methods using inhabited animal, fungi, and earthworm without current regulation.

      • 비젼 제어시스템에 사용된 카메라의 최적개수에 대한 실험적 연구

        장완식,김선호,김기영,임호진,안힘찬 朝鮮大學校 機械技術硏究所 2002 機械技術硏究 Vol.5 No.2

        The vision system model used for this study involves the six parameters that permits a kinds of adaptability in the relationship between the camera space location of manipulable visual cues and the vector of robot joint coordinates is estimated in real time. Also this vision control method requires the number of cameras to transform 2-D camera plane from 3-D physical space, and be used irrespective of location of cameras, if visual cues are displayed in the same camera plane. Thus, this study is to investigate the optimal number of cameras used for the developed vision control system according to the change of the number of cameras. This study is processed in the two ways : a) effectiveness of vision system model b) optimal number of cameras c) These results show the evidence of the adaptability of the developed vision control method using the optimal number of cameras.

      • 축구포지션별 무산소 운동능력 분석

        손장식,정정욱,김종호,임정수,김훈 대구대학교 기초과학연구소 2003 基礎科學硏究 Vol.19 No.3

        In this study, the Wingate power test was performed to investigate of the anaerobic power output in soccer players who play in different position. The main purpose of the test was to provide the enhancement of anaerobic capacity which was conducted by comparing and analysing of the Wingate test. This will improve the performance during the soccer games. Seventeen soccer players with three different positions, FW, MF, DF, were recruited, and undertook the Wingate power test, and anaerobic power, peak power output, anaerobic capacity, and fatigue index was analysed. There was a significant effect of a peak power output among positions showing MF>DF>FW (P<.05). MF revealed the highest peak power output at 15 and 30 seconds time points compared to DF and FW. As far as the players' positions were concerned, a relative anaerobic capacity showed a similar results with a peak power output (P<.05; MF>DF>FW). Unlike with these results, the fatigue index in different positions did not show any statistical significant results (P>.05). Based on these results, it was suggested that the systematical training program is required to perform a modern soccer, pressure and total soccer, in MF players. If the physical strength, especially power out, in MF players is enhanced, the results of the soccer games will be also improved.

      • KCI등재후보

        한번의 간질발작 후 혈청 Neuron-Specific Enolase 농도 변화

        최승호,박영춘,이장준,임정근,김지언,이상도 啓明大學校 醫科大學 1999 계명의대학술지 Vol.18 No.4

        An increase in neuron-specific enolase (NSE) levels in serum and CSF has been shown to be an useful marker of brain damage after stroke, global ischemia, and coma. We report the changes of serum NSE levels after seizure attacks in epileptic patients compared with the levels in normal controls and epileptic patients without seizure attack at least 7 days (epileptic controls). Twenty-four seizures were included in this study. Blood was drawn within 1 hour, at 12 hour, 24 hour, and 48 hour after seizure attack. Serum NSE levels were measured with radioimmunoassay. The mean NSE levels for normal controls and epileptic controls were 6.94 ng/ml and 7.46 ng/ml, respectively. There were significant increase in level of serum NSE measured within 1 hour after seizure attack in epileptics compared with the level in normal controls (15.10ng/ml versus 6.94ng/ml, p<0.05) and epileptic controls (15.10ng/ml versus 7.46ng/ml, p<0.05). Serum NSE measured at 12 hours after seizure also increased compared with normal controls (12.32ng/ml versus 6.94ng/ml, p<0.05) and epileptic controls (12.43ng/ml versus 7.46ng/ml, p<0.05). Between normal controls and epileptic controls, there were no significant difference in serum NSE levels. We conclude that serum NSE level was elevated in epileptic patients who had seizure attack within 12 hours. Serum NSE levels can be useful marker for seizure within 12 hours after onser. The elevated serum NSE level after single seizure attack may suggest that the brain was injured in single seizure.

      • KCI등재후보

        신경행동학적 증상 설문지의 타당도 및 신뢰도에 관한 연구

        권호장,조수헌,임현술 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.1

        유기용제 폭로에 의한 신경행동학적 장애를 선별 검사 할 수 있도록 고안된 설문지의 타당도와 신뢰도를 검정할 목적으로 유기용제를 사용하는 페인트 제조공장과 유기용제를 전혀 사용하지 않는 코일제조 공장의 근로자를 대상으로 2차에 걸쳐 설문조사를 실시하여 다음과 같은 결과를 얻었다. 1. 유기용제 폭로 근로자들이 비폭로 근로자들에 비해 증상총점의 평균이 통계적으로 유의하게 높았으며 이 결과는 나이, 근무기간, 교육연한, 음주, 흡연 등의 교란 요인을 통제하고도 유지되었다. 2. 신뢰도를 보기 위하여 페인트제조공장 근로자를 대상으로 2개월 간격으로 시행한 검사-재검사에서 문항별 kappa값은 0.15-0.69의 범위안에 있었으며 0,4이상으로 비교적 일치도가 높은 문항은 31문항 중 16문항이었다. 3. 검사문항간에 내적 일치도를 보기 위하여 Cronbach Alpha계수를 구한 결과 분포를 표준화하기 이전에는 0.85, 분포를 표준화한 이후에는 0.86의 값을 보였다. 이상의 결과를 볼 때 본 연구에 사용된 설문지는 유기용제 폭로로 인한 신경행동학적 장애를 비교적 잘 반영하고 있으며 신뢰도도 비교적 높은 것으로 판단되었다. There are many studies on the neurobehavioral symptoms due to organic solvent exposure using questionnaire. However there is little published evidence on validity and reliability of the questionnaire on the neurobehavioral symptoms. In present study, the authors tested the validity and reliability of our questionnaire, which was designed for screening neurobehavioral disturbance in organic solvent exposer. Questionnaire was administered to the workers of one paint manufacturing plant and one coil manufacturing plant. In order to evaluate validity of the questionnaire, the average questionnaire scores of two plants were compared. The average score of paint manufacturing plant were higher than that of coil manufacturing plant and the difference was statistically significant. After adjustment of age, duration of employment, education, smoking history and alcohol consumption, significance was maintained. Test-retest reliability was evaluated by kappa statistics. More than 50% of question items showed values of kappa above 0.4. Cronbach coefficient alpha which reflects internal consistency of the questionnaire was 0.86. Overall the data showed that validity and reliability of the questionnaire were generally acceptable.

      • 수산화마그네슘에 의한 배연탈황에 관한 연구

        정홍기,장성호,임찬섭,김장호 동아대학교 환경문제연구소 1995 硏究報告 Vol.18 No.2

        This study has been carried out to evaluate the effect on desulfurization of flue gas by Mg(OH)₂in steam-power cogeneration plant. We investigated the effects of desulfurizatin on the temperature of flue gas and pH of circulation water in aborber. The desulfurization installations of flue gas in cogeneration and thermoelectric power plant will be increased every year because of stricted environmental regulation in Korea. Some conclusions obtained are summerized as follows ; 1. With regard to running cost, Mg(OH)₂ absorbent for desulfurization of flue gas snowed more economic than Ca(OH)₂. 2. While bunker-C oil with 1.6% sulfur was used, desulfurization of flue gas by Mg(OH)₂ was performed and then SO₂ content in the exhausted gas was under 50 ppm. 3. In flue gas desulfurization, the optimum condition on the temperature and pH was 55-60℃ and 6 respectively, then efficiency was 96%.

      • 골격근 근형질세망의 ATPase활성도에 대한 Vanillylnonanamide의 영향

        박경섭,홍장희,류영수,성지연,허강민,임종호,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2

        To investigate the effect of vanillylnonanamide(VN) on the ATPase activity of the sarcoplasmic reticulum(SR) of the skeletal muscle, we prepared the SR vesicles from the back muscle of the rabbit, and measured ATPase activity. The results as follows: Ca-ATPase activity was about 50% in the total ATPase activity of skeletal muscular SR. In the reaction mixture with calcium, 100μM VN increased ATPase activity to 20%, and 100 nM and 10 μM thapsigargin(THP) inhibited the ATPase activity to 50% and 60%, respectively. And 100 μM VN plus 100 nM or 10 μM THP more inhibited ATPase activity than THP alone did it. However, without calcium, 100μM VN did not affect ATPase activity, and 10 μM THP inhibited it to 41%, but VN plus THP inhibited it to 31%. The above results show that VN in the reaction mixture with or without calcium has the different action to ATPase activity when it is used alone or together with Ca-ATPase inhibitor THP. This suggests that VN might increase or decrease the skeletal SR Ca-ATPase activity through affecting the lipid membrane around the Ca-ATPase.

      • 유방에서 기원된 전이성 췌장암종 1예 : A case report

        김윤신,이미숙,장원재,임성철,기근홍,전호종,서재홍 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

        Pancreas is known for the unfavorable soil to the metastatic carcinoma, and pancreatic metastasis is achieved after systemic dissemination was developed. The primary tumors of metastatic pancreatic carcinoma are breast, lung, Iymphoma, malignant melanoma, leukemia, stomach. and colon. The breast carcinoma is the commonest tumor to metastasize to pancreas and both tumors are related to estrogen receptor for tumor growth. In the present case, tumor cells had small round nucleus and showed indian file infltrating pattern and lack of cohesion, which are seen in infiltrative breast carcinoma. The patient was underwent radical mastectomy due to infiltrative ductal carcinoma three years ago. And also the tumor cells showed nuclear reactivity to immunostaining for estrogen receptor In combination of the histopathologic. immunohistochemical, and her past history, we concluded that breast carcinoma was metastasized to pancreas, and we report a case of metastatic pancreatic carcinoma from breast with review of literatures.

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