Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

Jin, H.J.,Kim, I.C.,Wee, M.S.,Yeon, S.H.,Kim, C.D.,Lee, S.S.,Cho, C.Y.,Cho, S.R.,Son, D.S.,Park, C.K. 韓國受精卵移植學會 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

HWE(Hot Wall Epitaxy)에 의한 CuGaSe₂/GaAs epilayer 성장과 광전기적 특성

홍광준,정준우,백형원,정경아,방진주,진윤미,김소형 조선대학교 기초과학연구소 2001 自然科學硏究 Vol.24 No.-

수평 전기로에서 CuGaSe₂ 다결정을 합성하여 HWE(Hot Wall Epitaxy) 방법으로 CuGaSe₂/GaAs epilayer를 반절연성 GaAs(100)기판 위에 성장하였다. CuGaSe₂/GaAs epilayer는 증발원의 온도를 610℃, 기판의 온도를 450℃로 하였다. CuGaSe₂/GaAs epilayer의 결정성의 조사에서 20K에서 광발광(photoluminescence) 스펙트럼이 672.6nm(1.8432eV)에서 엑시톤 방출 스펙트럼이 가장 강하게 나타났으며, 이중결정 X-선 요동곡선(DCRC)의 반폭치(FWHM)도 138 arcsec로 가장 작아 최적 성장 조건임을 알 수 있었다. Hall 효과는 van der Pauw 방법에 의해 측정되었으며, 온도에 의존하는 운반자 농도와 이동도는 293K에서 각각 4.87×10^(23) electron/m³, 1.29×10² m²/v-s 였다. CuGaSe₂/GaAs epilayer의 광전류 단파장대 봉우리들로부터 20K에서 측정된 ΔCr(Crystal field splitting)은 약 0.09 eV ΔSo(spin orbit coupling)는 0.2498 eV였다. 20K의 광발광 측정으로부터 고품질의 결정에서 볼 수 있는 자유 엑시톤 (free exciton)과 매우 강한 세기의 중성 받개(acceptor) 구속 엑시톤 (bound exciton)등의 피크가 관찰되었다. 이때 받개 구속 엑시톤 (bound exciton)의 반치폭과 결합에너지는 각각 8meV와 93.2meV 였다. 또한 Haynes rule에 의해 구한 불순물의 활성화 에너지는 466meV였다. The stochiometric mixture of evaporating materials for the CuGaSe₂/GaAs epilayer was prepared from horizontal furnace. Using extrapolation method of X-ray diffraction patterns for the polycrystal CuGaSe₂, it was found tetragonal structure whose lattice constant a_(0) and c_(0) were 5.615 Å and 11.025 Å, respectively. To obtain the CuGaSe₂/GaAs epilayer, CuGaSe₂ mixed crystal was deposited on throughly etched GaAs(100) by the Hot Wall Epitaxy(HWE) system. The source and substrate temperature were 610 ℃ and 450 ℃ respectively. The crystalline structure of CuGaSe₂/GaAs epilayer was investigated by the double crystal X-ray diffraction(DCXD). Hall effect on this sample was measured by the method of van der pauw and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by pizoelectric scattering in the temperature range 30 K to 150 K and by polar optical scattering in the temperature range 150 K to 293 K. From the photocurrent spectra by illumination of polarized light of the CuGaSe₂/GaAs epilaye. We have found that values of spin orbit coupling ΔSo and crystal field splitting ΔCr was 0.0900 eV and 0.2498 eV, respectively. From the photoluminescence measurement of CuGaSe₂/GaAs epilayer, we observed free excition(Ex) observable only in high quality crystal and neutral acceptor-bound exciton (A^(0); X) having very strong peak intensity. And, the full width at half maximum and binding energy of neutral donor bound excition were 8 meV and 93.2 meV, respectively. By Haynes rule, an activation energy of impurity was 466 meV.

The Effects of Stress Related Genes on Carcass Traits and Meat Quality in Pigs

H. J. Jin,B. Y. Park,J. C. Park,I. H. Hwang,S. S. Lee,S. H. Yeon,C. D. Kim,C. Y. Cho,Y. K. Kim,K. S. Min,S. T. Feng,Z. D. Li,C. K. Park,C. I. Kim 아세아·태평양축산학회 2006 Animal Bioscience Vol.19 No.2

The current study was conducted to investigate the relationship between stress related gene and meat quality in pigs. A total number of 212 three-way cross bred (Landrace-Yorkshire횞Duroc) and 38 Duroc were sampled from the Korean pig industry to determine genotype requency of porcine stress syndrome (PSS) and heat shock protein 70 kDa (HSP70) genes and their relationship with carcass traits and longissimus meat quality. Screen of HSP70 was performed by the single strand conformation polymorphism (SSCP) technique. Based on the analysis of restriction fragment length polymorphism (RFLP) in ryanodine receptor 1 (RYR1) gene, genetic disorder of PSS was related to a mutation at 18,168th (C to T) of exon 17. There was no significant difference in ultimate meat pH and backfat thickness between HSP70 K1-AA type and -BB type in pure Duroc breed. In Landrace-Yorkshire횞Duroc (L-YxD) cross bred pig, our results indicated that HSP70 derivate type in Duroc had a limited effect on backfat thickness, but L-YxD type had a noticeable linkage with HSP70 K1-AA and K3-AB. This tendency was also observed in hot carcass weight where HSP70 K1-AA and K3-AB resulted in heavier weight with 86.3 kg compared to HSP70 K1-AB and K3-BB of 74.3 kg. Results imply that stress related HSP70 genotype has a potential association with backfat thickness and carcass weight.

Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

H. J. Jin,I. C. Kim,M. S. Wee,S. H. Yeon,C. D. Kim,S. S. Lee,C. Y. Cho,S. R. Cho,D. S. Son,C. K. Park,Z. D. Li 사단법인 한국동물생명공학회 2007 한국동물생명공학회지 Vol.22 No.4

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire(p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

Expression of mosquitocidal crystal protein genes in non-insecticidal Bacillus thuringiensis subsp. israelensis

Gill, S.S.,Oh, H.W.,Lee, D.W.,Roh, J.Y.,Park, H.W.,Jin, B.R.,Je, Y.H.,Kang, S.K. 東亞大學校附設遺傳工學硏究所 1998 遺傳工學硏究 Vol.- No.5

J.Y.ROH,H.W.PARK,Y.H.JE,D.W.LEE,B.R.JIN, H.W.OH,S.S.GILL AND S.K.KANG.1997. Bacillus thuringiensis NTB-1 isolated from soil samples in Korea produces ovoidal parasporal inclusions with proteins of approximately 24-40kDa in size. Although serological study indicated that the isolate has a flagella (H) antigen identical with subsp. israelensis, it seemed to be non-insecticidal against Lepidoptera and Coleoptera as well as Diptera. To investigate the activity of non-insecticidal B.thuringiensis transformed with insecticidal crystal protein genes, cryIVD and cytA genes of B. thuringiensis subsp. morrisoni PG-14, highly toxic to mosquito larvae, were introduced into the isolate NTB-1. The expression of mosquitocidal crystal protein genes in NTB-1 was characterized by SDS-PAGE analysis and electron microscopy. The results showed that crystalline inclusions of host, CryIVD and CytA were stably expressed in the transformant. However, the mosquitocidal activity of transformant was similar to that of B. thuringiensis subsp. kurstaki Cry-B harbouring cryIVD and cytA genes, demonstrating that a synergistic effect by an interaction of both introduced insecticidal and resident non-insecticidal crystal proteins was not observed.

유전자치료 효율향상을 위한 세포특이적 Vector의 개발

김계영,남명진,유천권,진동훈,백묘아,박만석,심수경,이혜경,이병철,박미연 국립보건연구원 2000 국립보건원보 Vol.37 No.-

마이크로 센서용 다채널 전해질 분석기의 샘플챔버 개선 및 샘플상호간의 간섭현상 보정

박준경,성기웅,배상곤,진경찬,김명남,손병기,조진호 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

In this paper, the sample chamber for multichannel electrolyte analyzing system was designed and the correction algorithm for interference among samples was proposed. The sample chamber was designed to replace the ISFET sensor more easier. The principles of design were the structural independency of each channel and the shield of sample solution from air contact. The correction algorithm could reject the interference effect of mixed sample solution and compensate the voltage difference between single electrolyte solution and mixed electrolyte solution. To approve the proposed algorithm, the several experiments were carried out under the condition of mixed samples. We acknowledged that with our proposed algorithm, the sensor output influenced by mixed electrolyte solution was improved with measurement accuracy.

The ''self spin valve'' in oxygen stoichiometric SrRu_1-xFe_xO_3-δ epitaxial thin films

Toreh, K.R.N.,Kim, D.H.,Dash, U.,Phan, T.L.,Lee, B.W.,Jin, H.W.,Lee, S.,Park, B.H.,Park, J.Y.,Cho, M.R.,Park, Y.D.,Acharya, S.K.,Yoo, W.,Jung, M.H.,Jung, C.U. Elsevier Sequoia 2016 Journal of alloys and compounds Vol.657 No.-

SrRu<SUB>1-x</SUB>Fe<SUB>x</SUB>O<SUB>3-δ</SUB> (x = 0.00, 0.05, 0.10, and 0.20) thin films were fabricated to study the intrinsic aspects of a ''self spin valve''. Using epitaxial strain and high oxygen partial pressure during thin film growth, single phase thin films with negligible oxygen vacancies were successfully grown, and problems related to A-site disorder and grain boundaries were minimized. Under application of an external magnetic field of up to 9 T, the resistivity of all films decreased, resulting in large negative magnetoresistance (up to ~14.4%), which was stronger at temperatures in the range 10-30 K. An abrupt metal-insulator transition at T~ 43 K was found in the x = 0.20 film, which was explained using a two-fluid model related to electron-electron interactions. From the model, two fitting parameters were found to be necessary for in-situ and homogenous defects, while three or unphysical fitting parameters were necessary for ex-situ and inhomogeneous defects.

Synthesis and characterization of cauliflower-like ZnS microspheres by simple self-assembly method

Park, J.Y.,Park, S.J.,Lee, J.H.,Hwang, C.H.,Hwang, K.J.,Jin, S.,Choi, D.Y.,Yoon, S.D.,Lee, I.H. North-Holland 2014 Materials letters Vol.121 No.-

Cauliflower-like ZnS microspheres comprising ZnS nanoblock-like structures were fabricated by a simple one-step self-assembly method involving a hydrothermal process at 150<SUP>o</SUP>C for 10h using Zn(CH<SUB>3</SUB>COO)<SUB>2</SUB> and Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>.5H<SUB>2</SUB>O starting materials in an aqueous solution. The synthesized samples were characterized by field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), X-ray photoelectron spectrometer (XPS), and Brunauer-Emmett-Teller (BET) analysis. The photocatalysts were evaluated using the photodecomposition of methylene blue under UV light. The aqueous solutions had a pH 7, and exhibited the presence of a suspension of cauliflower-like ZnS microspheres, about 3-5μm in diameter constructed of ZnS nanoblocks <100nm size. These cauliflower-like ZnS microspheres have a surface area of 22.82m<SUP>2</SUP>/g, a pore volume of 0.0252cm<SUP>3</SUP>/g, and a pore diameter of 3.77nm, as determined by BET analysis. The photocatalytic degradation rate followed a pseudo-first-order equation. The kinetic constant (k<SUB>1</SUB>) of the cauliflower-like ZnS microspheres was 5.11x10<SUP>-2</SUP>min<SUP>-1</SUP>.

이성교제 중인 대학생의 사이버섹스 중독, 외로움 및 이성관계 만족에 관한 연구

김윤정,박지야,변수영,이겨레,이아름,이지영,임재희,조혜림,진진화,최재의,박효정,이혜림 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Objectives: The purpose of the study was to identify the relationships between cybersex addition, loneliness and relationship satisfaction among Korean college students. Methods: 293 students were conveniently selected. The instruments used in this study were cyber-sexual addiction index, loneliness scale and relationship assesment scale. ANOVA, t-test and Pearson correlation coefficient with SPSS 22.0 Version were used to analyze the data. Results: 8.9% of the participants had moderate or severe addiction to cybersex. The level of cybersex addiction is significantly influenced by gender, period of relationship, time taking to sexual relations, contact number of cyber pornography and contact time of cyber pornography. Significant association was observed between cybersex addition, loneliness and relationship satisfaction. Conclusions: The factors mentioned in this study as well as loneliness and relationship satisfaction were important factors. Therefore, future studies on this topics need to develope interventions to reflect cultural and physical character of college students.