In-line 마그네트론 스퍼터 장치를 사용하여 산소 분위기에서 제작한 Ag 박막의 특성

구대영,김원목,조상무,황만수,이인규,정병기,이택성,이경석,조성훈,Ku, Dae-Young,Kim, Won-Mok,Cho, Sang-Moo,Hwang, Man-Soo,Lee, In-Kyu,Cheong, Byung-Ki,Lee, Taek-Sung,Lee, Kyeong-Seok,Cho, Sung-Hun 한국재료학회 2002 한국재료학회지 Vol.12 No.8

A study was made to examine the electrical, compositional, structural and morphological properties of Ag thin films deposited by DC magnetron sputtering in $O_2$ atmosphere with deposition temperature from room temperature to 15$0^{\circ}C$ using in-line sputter system. The Ag films deposited at temperature above $100^{\circ}C$ in oxygen atmosphere gave a similar specific resistivity to and even lower oxygen content than those deposited using pure Ar sputter gas The Ag films deposited with pure Ar gas was mainly composed of crystallites with [111] preferred orientation, while, for those deposited in oxygen atmosphere, more than 50% of the volume was composed of crystallites with [100] orientation. The difference in the micro structure did not cause any difference in the specific resistivity of Ag films. The results showed that the transparent conducting oxide films and the Ag films could be processed sequentially in the same deposition chamber with careful control of deposition temperature, which might result in a cost reduction for constructing the large scale in-line deposition system.

스프레이 菊花의 왕겨경 循環式 養液栽培에서 養液의 濃度 및 供給回數의 영향

黃仁澤,金光秀,金月洙,金道翊,金正根 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

본 실험은 스프레이 국화 왕겨 배지를 이용한 순환식 양액재배에 있어서 양액의 적정농도와 공급횟수를 구명하고자 실시한 결과 양액 농도 처리에서는 원시표준액의 1.6배 처리구가 초장, 화경장, 경경, 화경수, 엽장, 엽폭, 절화중에서 가장 양호하였고, 1일 공급횟수는 표준농도(1.0배)는 12회가 좋았고 1.3배와1.6배 처리구에서는 6회공급구에서 절화품질이 좋았다. 양액농도별 개화기는 표준농도에 비해 1.3배는 2일, 1.6배 처리구는 3일정도 지연되었다. 양액 농도별 T-N함량은 1.3배 농도에서 많았고 양이온치환용량은 농도가 높은 1.6배 농도에서 높았으며 양액 농도별 엽록소 함량은 1.0배 농도에서 가장 높았다. This experiment was conducted to determine the optimal ionic strength of nutrient solution and fertigation frequency affecting growth and development of spray chrysanthemum (Dendranthema grandiflorum) in recycling system using rice hull. Growth parameters in terms of plant height, flowers diameter, stem diameter, number of flowers, leaf length and width and flower weight were shown better in thd 1.6 ionic strength of nutrient solution than those of 1.0 ionic strength of nutrient solution. Fertigation frequency was good at 12 times during day and night in the standard solution (1.0 ionic strength) while 6 times per day and night were good in both 1.3 and 1.6 strength of nutrient solution. Flowering time was postponed up to 2 day in the treatment of 1.3 ionic strength of nutrient solution, and 3 day in 1.6 ionic strength of nutrient solution. The content of total nitrogen was higher in the 1.3 ionic strength of nutrient solution. Cation exchange capacity was high in the 1.6 ionic strength of nutrient solution and chlorophyll content by the concentrations of nutrient solution was highest in the standard nutrient solution.

장시간 불가피한 공복(fasting)후 인체의 내분비 변화 : 삼풍백화점 붕괴 사고후 구조된 3인의 증례 THREE CASES IN COLLAPSE OF SAMPOONG DEPARTMENT STORE

박규남,황주일,박조현,오동렬,이원재,오승택,김세경,김인철 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

The interactions between nutrition and the endocrine system are more intimate and complex that previously thought. Nutrition modulates secretion and activity of many hormones, as part of the stress adaptation process. Fasting, in particular, produces a number of important changes in the endocrine system, with teleological purpose of helping the body tolerate the lack of food ingestion. These changes are therefore beneficial and protective, although at times they may not appear to be that way. The endocrine changes of fasting mediate the metabolic response. We must emphasize that these changes are completely reversible when appropriate nutrition is established. These changes are as followes: 1) decreased insulin and increased glucagon levels, 2) decreased thyroid effect, 3) decreased sympathetic activity, 4) hypothalamic hypogonadism, 5) decreased growth, 6) altered glucocorticoid secretion and metabolism, 7) impaired mineralocorticoid response, 8) decreased ADH secretion and effect. We experienced three cases of involuntary prolonged fasting after collapse of Sampoong department store. They were released from collapsed field after 11(case Ⅰ), 13(case Ⅱ), 17days(case Ⅲ) respectively. In each case, the endocrine changes of plasma levels were as followes: decrease of cortisol in case Ⅰ, increase of GH in case Ⅱ, decrease of free T3, T3, cortisol and increase of reverse T3, insulin in case Ⅲ. Each one did not show the same endocrine changes of plasma levels. But we think these changes of fasting are beneficial in metabolic response of human.

항생제 혼합 시멘트 정을 이용한 장관골 감염성 불유합의 이단계 치료

조세현,정순택,박형빈,황선철,하용찬,황인환 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적: 장관골 감염성 불유합의 치료 시 항생제 혼합 시멘트 정을 이용한 이단계 재건술의 결과를 분석하고자 한다. 대상 및 방법: 1998년 1월부터 2002년 9월까지 장관골 감염성 불유합으로 이단계 재건 수술을 받은 41예 (대퇴골 26예, 경골 15예)를 대상으로 하였다. 일단계 수술에서는 광범위한 변연 절제술, 부골 제거 및 세척술과 기존 고정 장치의 제거 또는 외고정 장치로의 교환을 시행한 후 시공 (dead space)과 불유합 주변 연부조직 내 항생제 혼합 시멘트 정을 삽입하였다. 임상적 감염 증상이 호전된 평균 8.7주 (3주~32주) 후 이단계 재건술로서 Ⅰ군은 내고정 장치 (금속판 5예, 골수강내 금속정 8예), Ⅱ군은 외고정 장치 (Ilizarov 25예, Monofixator 3예)로 각각 고정하고 17예에서는 골내 이동술이나 골 연장술을 시행하였다. 양군 모두 필요한 경우 골 이식과 시멘트 정의 교환을 추가하였다. 추시 기간은 16개월에서 최장 71개월로 평균 45개월 이었다. 치료 결과는 골유합 기간 및 Paley 방법에 의한 방사선상 골유합 상태와 하지 기능으로 평가하였다. 결과: Ⅰ군 13예 전부와 Ⅱ군 28예 중 26예에서 감염의 치료와 골유합을 얻었다. 이단계 재건술 후 골 이식술, 골 소파술, 외고정 핀 교환과 각변형 교정술, 피부 이식 또는 근육 피판 회전술 등의 보조적 시술이 Ⅰ군은 평균 2회, Ⅱ군은 평균 6.2회 추가되었다. 평균 골유합 기간은 Ⅰ군이 19.3주이고 Ⅱ군이 23.1주로 내고정군이 더 빨랐다. 방사선상 Paley 골유합 상태는 Ⅰ군이 우수 8예, 양호 5예이었고, Ⅱ군이 우수 18예, 양호 7예, 보통 1예, 불량 2예 이었다 (p=0.492). 기능적 결과는 Ⅰ군에서 우수 6예, 양호 6예, 보통 1예 이었고, Ⅱ군에서는 우수 10예, 양호 13예, 보통 3예, 불량 2예 이었다 (p=0.267). 결론: 항생제 혼합 시멘트 정의 골수염 치료 효과가 관찰되었으며, 감염이 호전된 후 내고정 장치로 교환해 준 군이 외고정을 유지한 군에 비하여 추가 수술 횟수가 적고, 골유합 기간도 빨랐다. Purpose: To evaluate treatment results between internal na external fixation groups in two-stage reconstruction of infected nonunion of long bones using antibiotics-impregnated cement beads. Materials and Methods: In the first stage, preexisting hardwares were removed and radical debridement was done. The dead space was filled with antibiotics -impregnated cement beads and the nonunion sits was immobilized by external fixation, cast or skeletal traction. In the second stage, all cases were divided into two groups; the nonunion was fixed by internal fixation in group Ⅰ versus external fixation in group Ⅱ. The intervening period between the first and second stage was average 8.7 weeks (range, 3~23 weeks). Results: The follow-up period was average 45 months (range, 16~71 months). Infection control and bone union were achieved in all 13 cases of group Ⅰ. Infection recurred in two of 28 cases in group Ⅱ, one underwent above-knee amputation and the other case was lost in follow-up. The mean number of supportive operations including repeated curettage, augmentation and change of infected pins, angular correction, and soft tissue flap was average 2 and 6.2 times respectively in group Ⅰ and group Ⅱ. Bony union period was average 19.3 and 23.1 weeks in each group. According to Paley's classification, group Ⅰ was similar to group Ⅱ in bony and functional result (p>0.05). Conclusion: Antibiotics-impregnated cement beads provided positive effect on infection control. Internal fixation group showed less number of additional operations and earlier bony union than external fixation group.

The Identification of Binding Mode for Arabidopsis thaliana 7-Keto-8-aminopelargonic Acid Synthase (AtKAPAS) Inhibitors

Cho, Jae-Eun,Kang, Sun-Young,Choi, Jung-Sup,Ko, Young-Kwan,Hwang, In-Taek,Kang, Nam-Sook Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

In this study, we determined the 3D-structure of Arabidopsis thaliana KAPAS by homology modeling. We then investigated the binding mode of compounds obtained from in-house library using computational docking methods. From the flexible docking study, we achieved high dock scores for the active compounds denoted in this study as compound $\mathbf{3}$ and compound $\mathbf{4}$. Thus, we highlight the flexibility of specific residues, Lys 312 and Phe 172, when used in active sites.

Prediction of the vacuum assisted resin transfer molding (VARTM) process considering the directional permeability of sheared woven fabric

Kim, Jae-In,Hwang, Yeon-Taek,Choi, Kyung-Hee,Kim, Hee-June,Kim, Hak-Sung Elsevier 2019 COMPOSITE STRUCTURES -BARKING THEN OXFORD- Vol.211 No.-

<P><B>Abstract</B></P> <P>In this study, the in-plane permeability of a woven composite was characterized with respect to the fiber orientation of the deformed woven fabric, to predict the vacuum assisted resin transfer molding (VARTM) process. An analytical model was suggested to predict the permeability of shear-deformed woven fabric and the model was compared to the experiment results. Based on the developed model, the VARTM process for the U-shaped composite structure was simulated using commercial software (Pam-Form and Pam-RTM). The simulation results were compared with that of the VARTM experiment. Considering the fiber directional permeability of the shear-deformed woven fabric, it was concluded that the VARTM simulation could accurately predict the VARTM process results such as the filling time and flow front pattern.</P>

Characterization of plasma gelsolin as a substrate for matrix metalloproteinases

Park, Sung-Min,Hwang, In Kwan,Kim, Se Yeon,Lee, Seo-Jin,Park, Kang-Sik,Lee, Seung-Taek WILEY-VCH Verlag 2006 Proteomics Vol.6 No.4

<P>We previously showed that plasma gelsolin, a major component of the extracellular actin scavenging system, is an matrix metalloproteinase (MMP)-14 substrate. Here we confirmed that plasma gelsolin is cleaved by MMP-14 at the plasma level, and found that it was most efficiently digested by MMP-3 followed by MMP-2, MMP-1, MMP-14, and MMP-9, in that order. Plasma gelsolin (90 kDa) was cut into several fragments of 43–48 kDa by MMP-3. The MMP-3 cleavage sites in plasma gelsolin were determined by labeling the C termini generated by in-gel digestion with 50% H<SUB>2</SUB><SUP>18</SUP>O combined with peptide mass mapping, and sequencing of the N-terminal amino acids. Plasma gelsolin was cleaved at Asn<SUP>416</SUP>-Val<SUP>417</SUP>, Ser<SUP>51</SUP>-Met<SUP>52</SUP>, and Ala<SUP>435</SUP>-Gln<SUP>436</SUP>.Proteolytic cleavage by MMP-3 resulted in considerable loss of its actin filament-depolymerizing activity. This suggests that MMPs weaken the extracellular actin-scavenging system by cleaving plasma gelsolin and may, therefore, be involved in pathological conditions induced by extracellular actin, such as endothelial injury, respiratory distress syndrome, hepatic necrosis, and septic shock.</P>

Depletion of adipocyte Becn1 leads to lipodystrophy and metabolic dysregulation

Yaechan Song,Young Jin,Yul Ji,Sung Sik Choe,Yong Geun Jeon,Heeju Na,Tae Wook Nam,Hye Jeong Kim,Hahn Nahmgoong,Sung Min Kim,Jae-woo Kim,Ki Taek Nam,Je Kyung Seong,Daehee Hwang,Chan Bae Park,In Hye Lee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Macroautophagy is a catabolic process that delivers damaged and unnecessary cytosolic contents to lysosomes for removal of defective subcellular organelles and proteins. Becn1 is a key regulator of autophagy, forming a complex with class III phosphatidylinositol 3-kinase (PI3K-III) to initiate autophagosome formation. Although Becn1 has been implicated in numerous diseases such as cancer, aging, and neurodegenerative disease, its function in mature adipocytes remains elusive. In this study, we implemented Adipoq-Cre to generate adipocyte-specific Becn1 KO (BaKO) mice to identify the function of autophagy in adipose tissue homeostasis. BaKO mice naturally developed severe lipodystrophy and metabolic dysregulation, which were exacerbated upon high dietary fat intake. These mice also acquired adipose tissue inflammation, hepatic steatosis, and insulin resistance which advanced to early mortality. Immortalized stromal vascular cells (imSVCs) were established in-vitro to conditionally knock-out Becn1 upon tamoxifen treatment. Ablation of Becn1 in adipocytes led to programmed cell death in a cell-autonomous manner, accompanied by elevated endoplasmic reticulum (ER) stress gene expression. Furthermore, we observed that Becn1 depletion sensitized mature adipocytes to ER stress through activation of protein kinase R-like ER kinase (PERK) – eukaryotic initiation factor 2α (eiF2α) axis. This led to excessive unfolded protein response (UPR) and accelerated cell death through notable induction of C/EBP homologous protein (CHOP) and Bax expression. Taken together, these data suggest that adipocyte-Becn1 would serve as a crucial player for adipocyte survival and adipose tissue homeostasis.

결핵균체 추출액으로부터 알파 및 베타단백항원의 정제에 관한 연구

황인택,백태현,김화중,박정규,최대경 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

It is well known that α- and β-antigens are major protein products of Mycobacterium tuberculosis. Alpha and beta antigens from the cell extract of Mycobacterium tuberculosis were purified by 80% ammonium sulfate precipitation, gel filtration of Sephacryl S-300, DEAE-Sephacel ion exchange chromatography and hydroxylapatite chromatography. Beta antigen was purified further by column preparative polyacrylamide gel electrophoresis. The purified alpha and beta antigens were checked degree of purified by SDS-polyacrylamide gel electrophoresis. The results were summarized as follows: 1. Alpha antigen was purified by ammonium sulfate precipitation, gel filtration, ion exchange chromatography and hydroxylapatite chromatography. The purified alpha antigen showed a distinct band of 30 KDa molecule on SDS-polyacrylamide gel electrophresis. 2. Beta antigen was purified by ammonium sulfate precipitation, gel filtration, ion-exchange chromatography, hydroxylapatite chromatography and column preparative polyacrylamide gel electophoresis. The purified beta antigen showed two distinct bands of 83 KDa and 52 KDa molecules on SDS-PAGE. 3. Especially, Beta antigen was absorbed well to hydroxylapatite column but alpha antigen was passed completely without absorption.

PVC 및 Olefin계 Copolymer의 열안정성에 관한 연구(II) : 열안정 영향 인자 Thermal Stability Factor

송해영,황택성,맹기석,박인환 忠南大學校 産業技術硏究所 1986 산업기술연구논문집 Vol.1 No.2

Several structural factors which governs the thermal stability of poly(vinyl chloride) were investigated by IR and H¹-NMR spectroscopy. PVC-Olefin copolymers having diverse structure were prepared by same reaction conditions such as reaction temperature and comonomer composition. It was found that keto allyl groups in the polymer backbone which were formed through a side reaction show the most pronauncing effect. The content of keto allyl group appeared to increase with the relative amount of olefin comonomers. On the other hand, neither chain branching nor tecticity influenced the thermal stability of copolymer. Also, the content of double bond fromed at chain ends was unrelated to the thermal stability.