Serial Changes of Heat Shock Protein 70 and Interleukin-8 in Burn Blister Fluid

( Kicheol Yoo ),( Kang Yeol Suh ),( Gi Hun Choi ),( In-suk Kwak ),( Dong Kook Seo ),( Dohern Kym ),( Hyeon Yoon ),( Yong Se Cho ),( Hye One Kim ) 대한피부과학회 2017 Annals of Dermatology Vol.29 No.2

Background: It has been reported that heat shock protein 70 (HSP70) and interleukin-8 (IL-8) play an important role in cells during the wound healing process. However, there has been no report on the effect of HSP70 and IL-8 on the blisters of burn patients. Objective: This study aimed to evaluate the serial quantitative changes of HSP70 and IL-8 in burn blisters. Methods: Twenty-five burn patients were included, for a total of 36 cases: twenty cases on the first day, six cases on the sec-ond, five cases on the third, three cases on the fourth, and two cases on the fifth. A correlation analysis was performed to de-termine the relationship between the concentration of HSP70 and IL-8 and the length of the treatment period. Results: The HSP70 concentration was the highest on the first day, after which it decreased down to near zero. Most HSP70 was generated during the first 12 hours after the burn accident. There was no correlation between the concen-tration of HSP70 on the first day and the length of the treat-ment period. No measurable concentration of IL-8 was de-tected before 5 hours, but the concentration started to in-crease after 11 hours. The peak value was measured on the fourth day. Conclusion: While HSP70 increased in the first few hours and decreased afterwards, IL-8 was produced after 11 hours and increased afterward in burn blister fluid. These findings provide new evidence on serial changes of in-flammatory mediators in burn blister fluid. (Ann Dermatol 29(2) 194∼199, 2017)

Application of Vendor-Neutral Iterative Reconstruction Technique to Pediatric Abdominal Computed Tomography

Woo Hyeon Lim,Young Hun Choi,Ji Eun Park,Yeon Jin Cho,이승현,Jung-Eun Cheon,Woo Sun Kim,In-One Kim,Jong Hyo Kim 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.9

ObjectiveTo compare image qualities between vendor-neutral and vendor-specific hybrid iterative reconstruction (IR) techniques for abdominopelvic computed tomography (CT) in young patients. Materials and MethodsIn phantom study, we used an anthropomorphic pediatric phantom, age-equivalent to 5-year-old, and reconstructed CT data using traditional filtered back projection (FBP), vendor-specific and vendor-neutral IR techniques (ClariCT; ClariPI) in various radiation doses. Noise, low-contrast detectability and subjective spatial resolution were compared between FBP, vendor-specific (i.e., iDose1 to 5; Philips Healthcare), and vendor-neutral (i.e., ClariCT1 to 5) IR techniques in phantom. In 43 patients (median, 14 years; age range 1–19 years), noise, contrast-to-noise ratio (CNR), and qualitative image quality scores of abdominopelvic CT were compared between FBP, iDose level 4 (iDose4), and ClariCT level 2 (ClariCT2), which showed most similar image quality to clinically used vendor-specific IR images (i.e., iDose4) in phantom study. Noise, CNR, and qualitative imaging scores were compared using one-way repeated measure analysis of variance. ResultsIn phantom study, ClariCT2 showed noise level similar to iDose4 (14.68–7.66 Hounsfield unit [HU] vs. 14.78–6.99 HU at CT dose index volume range of 0.8–3.8 mGy). Subjective low-contrast detectability and spatial resolution were similar between ClariCT2 and iDose4. In clinical study, ClariCT2 was equivalent to iDose4 for noise (14.26–17.33 vs. 16.01–18.90) and CNR (3.55–5.24 vs. 3.20–4.60) (p > 0.05). For qualitative imaging scores, the overall image quality ([reader 1, reader 2]; 2.74 vs. 2.07, 3.02 vs. 2.28) and noise (2.88 vs. 2.23, 2.93 vs. 2.33) of ClariCT2 were superior to those of FBP (p < 0.05), and not different from those of iDose4 (2.74 vs. 2.72, 3.02 vs. 2.98; 2.88 vs. 2.77, 2.93 vs. 2.86) (p > 0.05). ConclusionVendor-neutral IR technique shows image quality similar to that of clinically used vendor-specific hybrid IR technique for abdominopelvic CT in young patients.

GOIOVE-B 위성 E5a Secondary Code 결정 및 신호처리 결과

주인원 ( In One Joo ),신천식 ( Chun Sik Shin ),이사욱 ( Sang Uk Lee ),김재훈 ( Jae Hoon Kim ) 한국항공운항학회 2008 한국항공운항학회지 Vol.16 No.4

GIOVE-B is the second experimental Galileo satellite for the Galileo In-Orbit Validation, which was launched by a Soyuz/Fregat rocket departing from the Baikonur cosmodrome in Kazakhstan on 27 April and now operated successfully. This paper presents the results obtained from processing of the E5a signal transmitted from the GlOVE-B. The acquisition and tracking of the data and pilot channels are performed by the E5a software receiver implemented by ETRI. Moreover, the paper suggests the GIOVE-B E5a-I/ESa-Q secondary code, which is determined by analyzing the correlation output of the primary correlator using the primary code.

간아세포종 환아의 임상적 특성과 예후

김민영,김대연,안효섭,김종재,김인원,정성은,이성철,박귀원,김우기,Kim, Min-Young,Kim, Dae-Yeon,Ahn, Hyo-Seep,Kim, Chong-Jai,Kim, In-One,Jung, Sung-Eun,Lee, Seong-Cheol,Park, Kwi-Won,Kim, Woo-Ki 대한소아외과학회 1997 소아외과 Vol.3 No.2

Hepatoblastoma is a rare pediatric malignancy which frequently presents at an advanced un resectable stage. With the neoadjuvant chemotherapy, improved resectability and survival have been reported. Twenty children with biopsy proven hepatoblastoma were treated during the period between January 1987 and June 1995. Median age at diagnosis was 13 months(2 months to 7 year and 10 months), and 13 were male. Histologic profile was 13 epithelial(5 fetal, 4 mixed, 1 embryonal, 3 undetermined), and 5 mixed mesenchymal and epithelial and 2 of undetermined type. Chemotherapy effectively reduced the tumor volume($p$=0.008), and was able to convert 7 out of 9 initially unresectable cases(78%) to resectable ones. Twelve radical and 2 palliative operations were done with or without adjuvant chemotherapy. The Median follow up period was 33 months and the median survival was 26 months. The group with curative resection had a 61.1% 5 year survival rate, but none of palliative resection group survived more than 13 months($p$=0.0001). In univariate analysis for prognostic factors revealed, large tumor size at diagnosis and abscence of thrombocytopenia were associated with poor survival, but these differences were not statistically significant. Histological pure fetal type did not mean a better prognosis. Even with a recent neoadjuvant chemotherapy, the strategy should be focused on the radical resection as early as possible.

소아의 신혈관성 고혈압

강병철,하일수,김인원,정해일,최용,고광욱,Kang Byoung-Chul,Ha Il-Soo,Kim In-One,Cheong Hae-Il,Choi Yong,Ko Kwang-Wook 대한소아신장학회 1997 Childhood kidney diseases Vol.1 No.2

목적 : 소아 신혈관성 고혈압의 임상적 특징과 원인질환, 검사 소견 및 치료에 대한 반응 등을 분석하고자 본 연구를 시행하였다. 방법 : 1986년 1월부터 1994년 6월까지 서울대학교 어린이병원 소아과에 입원한 고혈압 환자 중 혈관조영술상 신혈관성 고혈압으로 진단된 16례에 대하여 병록지 검토를 통한 후향적 고찰을 시행하였다. 결과 : 전체 16례중 남녀비는 7:9이었고, 평균 연령은 8년 6개월이었다. 원인질환으로는 Takayasu 동맥염이 6례, Moyamoya병에 동반된 예가 5례, 섬유근성 이형증이 3례, 신경섬유종증에 동반된 예가 1례였으며, 1례에서는 원인 질환을 밝혀낼 수 없었다. 흔한 증상으로는 호홉곤란, 편측마비, 두통, 오심 등이 있었고, 신체검사 소견상 심비대, 간비대, 복부 청진상 잡음 등이 관찰되었다. 말초혈장 renin 활성도는 검사를 시행한 14례에서 모두 상승되어 있었다. 혈관조영술상 16례 중 9례가 양측성, 7례가 일측성 병변을 보였다. Captopril 신스캔은 시행된 전례에서 혈관조영술상의 병변과 일치되는 결과를 보였다. 약물치료만을 시행한 5례 중 2례에서는 혈압의 완전 정상화, 3례에서는 부분적 조절의 반응을 보였다. 경피적 혈관성형술은 10례에서 시도되었으나 5례에서는 실패하였고 성공한 5례 중 3례에서 재협착 또는 동반된 대동맥 협착등으로 다른 형태의 치료가 필요하였다. 수술적 치료는 4례에서 시행되었으며 모두 다른 형태의 치료로 실패한 경우였다. 이 중 3례에서는 혈압 조절에 실패하였고 1례에서는 부분적 조절의 반응을 보였다. 결론 : 소아의 신혈관성 고혈압의 원인 질환은 Takayasu 동맥염뿐 아니라 Moyamoya병, 섬유근성 이형증등 다양하므로 이들간의 감별이 필요하다. 여러 진단적 검사중 단독으로 확진 가능한 검사는 없으며 환자마다 적합한 진단적 검사법을 개별적으로 적용해야 한다. 치료로서 약물요법, 혈관성형술, 수술적 치료 등이 시행되지만 완치율 및 성공률은 아직 만족스럽지 못하다. Purpose : The clinical characteristics of renovascular hypertension (RVHT) in children were analyzed. Methods : Medical records of 16 children diagnosed as RVHT on the basis of angiography during Jan. '86 to Jun. 94 in our hospital were reviewed retrospectively. Results : The mean age at the onset was 8.5 yrs and the sex ratio(M:F) was 7:9. The causes of RVHT were Takayasu arteritis in 6, Moyamoya disease in 5, and fibromuscular dysplasia in 3 patients. Abdominal bruit was noted in 6 patients (38%). Peripheral renin activity was raised in all tested patients. Bilateral renal arterial involvemnent was found in 9 patients (56%). Captopril renal scans showed good correlation with angiographic findings. Five patients were treated with antihypertensives only, and blood pressure was controlled completely in 2 and incompletely in 3. Percutaneous transluminal angioplasty was performed in 10 patients with 50% of success rate. However, hypertension was recurred due to restenosis or accompaning aortic stenosis in 3 patients. Surgical treatment was performed in 4 patients, and the blood pressure was controlled partially in 1 and poorly in the remaining 3. Conclusions : Takayasu arteritis, Moyamoya disease and fibromuscular dysplasia are the major causes of childhood RVHT in our country. The diagnosis of RVHT in children should be based on a set of tests individually selected for case by case. For the low curability of the current treatment modalities available, RVHT in children should not be regarded as 'curable' so far. We expect, however, that the outcome will be improved by more extensive application of the newly developed surgical technique.

Intracranial and Spinal Ependymomas:Review of MR Images in 61 Patients

Ja-Young Choi,Kee-Hyun Chang,유인규,Keon Ha Kim,Bae Joo Kwon,Moon Hee Han,In-One Kim 대한영상의학회 2002 Korean Journal of Radiology Vol.3 No.4

Objective: To compare the age distribution and characteristic MR imaging findings of ependymoma for each typical location within the neuraxis. Materials and Methods: During a recent eleven-year period, MR images of 61 patients with histologically proven ependymomas were obtained and retrospectively reviewed in terms of incidence, peak age, location, size, signal intensity, the presence or absence of cyst and hemorrhage, enhancement pattern, and other associated findings. Results: Among the 61 patients, tumor location was spinal in 35 (57%), infrartentorial in 19 (31%), and supratentorial in seven (12%). In four of these seven, the tumor was located in brain parenchyma, and in most cases developed between the third and fifth decade. Approximately half of the infratentorial tumors occurred during the first decade. The signal intensity of ependymomas was nonspecific, regardless of their location. A cystic component was seen in 71% (5/7) of supratentorial, 74% (14/19) of infratentorial, and 14% (5/35) of spinal cord tumors. Forty- nine percent (17/35) of those in the spinal cord were associated with rostral and/or caudal reactive cysts. Intratumoral hemorrhage occurred in 57% (4/7) of supratentorial, 32% (6/19) of infratentorial, and 9% (3/35) of spinal cord tumors. In 17% (6/35) of spinal ependymomas, a curvilinear low T2 signal, suggesting marginal hemorrhage, was seen at the upper and/or lower margins of the tumors. Peritumoral edema occurred in 57% (4/7) of supratentorial, 16% (3/19) of infratentorial and 23% (8/35) of spinal cord tumors. Seventy-two percent (5/7) of supratentorial and 95% (18/19) of infratentorial tumors showed heterogeneous enhancement, while in 50% (17/34) of spinal cord tumors, enhancement was homogeneous. Conclusion: Even though the MR imaging findings of ependymomas vary and are nonspecific, awareness of these findings, and of tumor distribution according to age, is helpful and increases the likelihood of correct preoperative clinical diagnosis.

급성 파종성 뇌척수염(acute disseminated encephalomyelitis)의 임상양상 및 뇌자기공명영상의 진단적 의의

조성민(Sung Min Cho),김기중(Ki Joong Kim),황용승(Yong Seong Hwang),이시경(See Kyung Lee),김인원(In-One Kim) 대한소아신경학회 1994 대한소아신경학회지 Vol.2 No.1

(1) 연구 배경: ADEM은 중추신경계의 급성 탈수초성 염증성 질환으로 뇌자기공명영상의 이용으로 진단이 용이해졌다. 향후 본 질환의 진단과 추적에 도움이 되고자 본 연구를 시행하였다. (2) 방법: 1989년 3월부터 1994년 6월까지 서울대학교 어린이병원 소아과에 입원한 ADEM 환아 10명을 대상으로 하였고, 의무 기록을 토대로 임상 양상과 뇌자기공명영상 등의 검사 소견을 조사하였다. (3) 결과: 증상 발현 당시의 연령은 8개월에서 10세였고 남아가 2례, 여아가 8례였다. 선행하는 감염으로는 비특이적 상기도 감염이 6례, 홍역이 1례였고, 예방 접종으로는 일본 뇌염 예방 접종의 병력을 가진 경우가 2례였다. 신경학적 증상의 발현까지의 기간은 1주일에서 4주였으며 1주 이내가 5례, 1주에서 4주 사이가 4례였다. 신경학적 증상은 의식 장애가 5례, 대마비, 경련, 진전과 신경성 방광이 각각 3례, 편마비, 시각 장애, 구음 장애와 두통 등이 각각 2례였고, 그 외 안면 신경 마비, 반향 언어, 망각증 등이 각각 1례였다. 뇌자기공명영상은 전례에서 다발성의 백질을 침범하는 소견을 보였으며, T2 증강영상에서 백질의 고음영으로 나타났다. 대놔 백질의 병변을 보인 경우가 9례로 그 중에는 편층성 비대칭적 병변도 2례 있었으며, 소뇌 백질의 병변을 보인 경우가 4례로 이 중 대칭적 병변이 3례였다. 그 외의 침범 부위로는 뇌간과 내포가 각각 2례, 기저핵, 시상의 병변이 각각 1례였다. Gd-DTPA 조영상 조영 증강을 보인 경우는 2례였다. 뇌자기공명영상의 추적 촬영은 9례에서 시행하였고 8례에서 부분적 또는 완전한 병변의 소실이 관찰되었다. 전례에서 부신 피질 호르몬을 사용하였고, 항경련제를 사용한 경우도 2례 있었다. 2개월에서 3년간의 추적이 가능했던 8명 중 신경학적 증상이 남아있는 경우는 4례로 이들은 편마비와 진전, 경련, 기억과 계산의 장애, 경한 인격 장애를 각각 보였고 점진적 호전을 관찰할 수 있었다. (4) 결론: 종래에 임상 양상만으로는 뇌염과의 감별의 어려웠고 뇌전산화 단충촬영도 도움이 되지 않은 경우가 많았으나 최근 뇌자기공명영상의 이용으로 ADEM의 진단이 용이해졌으며, 그 추적 검사는 다발성 경화증과의 감별과 ADEM의 임상적 추적에도 매우 유용하게 사용될 수 있다. (1) Background. Acute disseminated encephalomyelitis is an acute demyelinating autoimmune inflammatory disease of CNS and develops after infection or vaccination. The diagnosis has been dependent on clinical features or autopsy findings before the use of brain MRI. We performed this study to investigate its clinical features and evaluate the usefulness of brain MRI for its diagnosis and follow-up. (2) Methods. Ten cases(2 males and 8 females) of acute disseminated encephalomyelitis who were diagnosed in the department of pediatrics. Seoul National (3) Results. The age of onset of symptoms was between 8 months and 10 years. The preceding events were nonspecific upper respiratory infection in 6 cases. Japanese B encephalitis vaccination in 2 cases. measles in 1 case. In one case there was no preceding event. The neurologic symptoms were altered consciousness. paraplegia, hemiplegia, seizure, tremor, visual disturbance and voiding difficulty. Brain MRI showed relatively symmetrical, multifocal high signal intensity lesions on T2-weighted image mainly in the cerebral or the cerebellar white matter. the brainstem, internal capsules or less frequently in the basal ganglia. Gd-DTPA enhancing lesions were found in 2 cases. Follow-up brain MRI demonstrated partial to complete resolution of the lesions except one case. All patients were managed on corticosteroid therapy. Of the 8 patients who were followed up for more than 2 months. the residual neurologic symptoms were hemiplegia and tremor in 1 case, seizure in 1 case. personality change in 1 case, disturbance of memory and calculation in 1 case. There were no residual neurologic symptom in 4 case. (4) Conclusion. Brain MRI is useful for the diagnosis and the follow-up clinical course of ADEM.

Tumors in the cerebellopontine angle in children: warning of a high probability of malignancy.

Phi, Ji Hoon,Wang, Kyu-Chang,Kim, In-One,Cheon, Jung-Eun,Choi, Jung Won,Cho, Byung-Kyu,Kim, Seung-Ki M. Nijhoff ; Kluwer Academic Publishers 2013 Journal of neuro-oncology Vol.112 No.3

<P>Cerebellopontine angle (CPA) tumors are uncommon in children, and the pathological spectrum is different from that of adults. In this study, we reviewed the pathological diagnosis of pediatric patients with a CPA tumor to determine the pattern in this age group. In a cohort of 267 patients with posterior fossa tumor, tumor locations were determined with preoperative magnetic resonance imaging (MRI). The pathological diagnosis, imaging characteristic, and treatment outcomes of patients with CPA tumors was reviewed and analyzed. Twenty-six patients (9.7 %) had a tumor in the CPA. The pathological spectrum was wide, from malignant intrinsic brain tumors to benign extra-axial tumors and sarcomatous lesions. Eighteen patients (69 %) had malignant tumors. The pathological nature was strongly linked to patient age. The mean age of malignant tumor group was significantly younger than that of benign tumor group. MRI findings that favored malignant histology included a plastic feature of the tumor, multiple signal voids, encasement of major arteries, widening of lateral recess, focal cerebellar edema, and hydrocephalus. The presence of seeding in the neuraxis also indicated malignant pathology. Especially, increased density on precontrast computed tomography was a strong predictor of malignant pathology. Malignant CPA tumors showed high surgical morbidity rate and grim long-term prognosis. Patient age and tumor location are the two most important clues for the diagnosis of any brain tumor. Unlike in adult patients, clinicians should expect a high probability of malignant histology for pediatric CPA tumors, especially in infants and young children.</P>

아토피피부염 병변에서의 CCL1의 특이적 발현

안인수 ( In Su Ahn ),김혜원 ( Hye One Kim ),박천욱 ( Chun Wook Park ),이철헌 ( Cheol Heon Lee ) 대한피부과학회 2011 大韓皮膚科學會誌 Vol.49 No.5

Background: Chemokines represent a superfamily of small cytokine-like chemoattractive proteins, which regulate leukocyte trafficking under homeostatic and inflammatory conditions. There have been previous reports that chemokine (C-C motif) ligand 1 (CCL1), a member of the family of chemoattractive proteins, is increasingly expressed in atopic dermatitis. Objective: We aimed to evaluate the quantity and the pattern of CCL1 expression in atopic dermatitis (AD). Methods: Biopsy specimens were taken from atopic skin and normal-appearing skin of AD patients as well as psoriatic skin of psoriasis patients. Quantitative real-time PCR analyses and immunohistochemistry of CCL1 expression were performed, and the quantity of CCL1 expression in acute AD was compared with those of normal-appearing atopic skin and psoriatic skin. The serum level of CCL1 was measured by ELISA. Results: CCL1 was most often expressed in acute atopic skin lesions, and the absolute amount of CCL1 18s rRNA in lesional atopic skin was 14.5-fold higher than that in non-lesional atopic skin. Moreover, CCL1 was expressed within the basal layer of the epidermis as well as in the dermis of the lesional atopic skin. However, CCL1 was expressed mostly in the dermis. Conclusion: Therefore, CCL1 represents a chemokine that is associated with flare-up of AD, and it may play an important role as a trigger of AD in the initiation and amplification of atopic skin inflammation in the acute phase. (Korean J Dermatol 2011;49(5):393∼397)

소아의 비사구체성 혈뇨와 연관된 Nutcracker 증후군

박경미,김우선,민재홍,최용,정해일,하일수,백경훈,김정수,김인원 대한신장학회 1998 Kidney Research and Clinical Practice Vol.17 No.5

Purpose: This study was designed to aid the diagnosis and to predict the outcorne by understanding the clinical course of nutcracker syndrome in childhood. Methods: The clinical, laboratory, radiological and cystoscopic data from the medical records of eleven children who were diagnosed as nutcracker syndrome by gross hematuria and pressure gradient criteria($gt;3mrnHg) were studied retrospectively and analyzed. Results: Sex ratio of the cases was 7:4, and the median age of onset was 12.8(3-14.3) years. Six cases showed persistent and 5 cases manifested interrnittent, exercise induced hematuria. Left flank pain(64%), abdominal pain(18%), left varicocele(9%) were associated in some of the children, but hematuria was the only symptom in 36Yo. Left renal vein entrapment was documented in 10 cases by ultrasonography. Out of the 5 cases studied by renal Doppler ultrasonography, 4 and 5 cases showed higher($gt;5) mean left renal vein diameter ratio(Distal/ Aortomesenteric portion) and mean peak velocity ratio respectively. Unilateral bleeding from left ureteral orifice was documented in 7 of the 9 cases at cystoscopy. The mean pressure gradient between proximal left renal vein and inferior vena cava was 4.4±1.6(3-7) mmHg. Hematuria of 25% and 57% of the cases disappeared spontaneously in 3 and 5 years after onset respectively. Proteinuria disappeared in 3 of the 5 initial proteinuric cases. Conclusion: Nutcracker syndrome must be considered in the differential diagnosis of non-glomerular, especially gross hematuria in childhood, and Doppler ultrasonography can aid diagnosis non-invasively. The renal function remained stable, but 4396 of the cases continued to show hematuria still 5 years after onset.