인간 재조합 인터루긴-32 면역조절작용에 대한 유세포 분석

이광수,김영관,채정일,심정현,김은미,강형식,김수현,윤도영,명평근 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organ available for human transplantation. however, it remains various hurdles for clinical xenotransplantation. In pig and mouse xenotransplantation, porcine xenograft evoke a strong cellular rejection response in immunocompetent host and grafts are destroyed within a week. This cellular immune response could involved both T cells and NK cells. A number of groups have shown that human NK cells can recognize and damage porcine endothelial cells. In addition, human T cells can respond to porcine endothelial cells through both direct and indirect mechanisms. Cellular rejection of porcine tissues requires T cells, particularly CD4^(+) cells. A new cytokine recombinant human interleukin-32α,β(IL-32α,β) has a role innate and acquired immune system. In order to investigate the role of recombinant mouse IL-18 and recombinant human IL-32α,β in xenograft rejection, we transplanted the PK(15) cells to C57BL/6 mice with or without intraperitoneal injection of recombinant mouse IL-18 or recombinant human IL-32 α,β. It was analyzed the population of NK cell, T cell and B cell in the C57BL/6 mice transplanted with PK(15) cells and recombinant mouse IL-18 or recombinant human IL-32α,β by flow cytometry analysis. As a result, lymph node and thymus of PK15/IL18, PK15/IL32α and PK15/IL32β injected group were increased to T cell activation population than normal injected groups. CD8^(+) T cells were decreased in lymph node of PK15/IL18, PK15/IL32α and PK15/IL32β injected groups. CD4^(+) T cells were increased in lymph node cell of PK15/IL32α and PK15/IL32β injected group and also, B cell population were increased in lymph node cell and spleen of PK15/IL18, PK15/IL32α and PK15/IL32β injected group. Therefore, we suggest that recombinant mouse IL-18 and recombinant human IL-32α,β suppress xenograft rejection in cellular xenotransplantation.

SLM에 의한 카드뮴의 추출에 관한 연구

김영일,신현심,박동원 동아대학교 환경문제연구소 1994 硏究報告 Vol.17 No.1

Liquid-liquid separation of Cd(Ⅱ) by SLM with Hollow fiber module is one of the most effective method for removal, separation of heavy metals in eque ous solution and waste water. Liquid-liquid separation by SLM using tri-n-octyl amine(TOA) was studied in order to investigate the optimum separation condition of Cd(Ⅱ) and overall coefficient of mass transfer. The experimental result was that separation of Cd(Ⅱ) in aqueous solution accomplished very well and overall coefficient of mass transfer was accordance experimental values with theoretic values.

연령 및 가정에서의 인터넷 환경이 인터넷 이용행태에 미치는 영향

이현주,심성훈,신동일 한국항공대학교 경영연구소 2001 경영연구 Vol.8 No.1

인터넷이 확산되면서 정보접근에 있어 사회 계층별로 더 큰 격차를 보이게 되었다. 초고속 인터넷 접속서비스의 급속한 보급으로 우리나라가 세계 최고 수준의 인터넷 이용률을 보이게 되면서 가정에서의 초고속 인터넷 접속 서비스 이용여부가 가구원 개인의 정보이용에 적잖은 영향을 미치고 있으며, 또한 연령대에 따라서도 많은 차이를 보이고 있다. 본 연구에서는 실증분석을 통해 이 두 변수에 의한 집단구분이 인터넷 이용장소, 이용시간, 이용빈도, 즐겨 찾는 사이트, 전자상거래 이용경험과 같은 인터넷 이용행태에 유의적인 차이를 발생시킴을 보이고 있다. The growth of internet use makes larger difference in information access among social classes. Rapid diffusion of broadband internet access at home enables Korea the highest internet penetration among OECD countries. So the accessibility of broadband service effects a lot on the information usage and also great difference with age. This study shows that there is significant difference in internet access location, time, frequency of access, favorite site and use of e-commerce according to two variables through empirical investigation.

고량강의 항염증 작용에 대한 연구

박히준,이제현,김수영,심범상,구헌종,강전모,최일환,이재동,김남재,이지숙,임사비나 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objective : The use of herbal therapy is becoming an increasingly attractive approach for the treatment of various inflammatory disorders. The Alpiniae officinari Rhizoma is popular in Aisa as a traditional herbal medicine. Alpiniae officinari Rhizoma is a species of the ginger family(Zingiberacease). Method : This study was performed to investigate the anti-inflammatory effect of Alpiniae officinari Rhizoma extract by the methods of "carrageenan induced paw edema" and "Lipopolysaccharide-induced inflammatory mediators in mouse macrophage RAW 264.7 cells". Result : We suggest that Alpiniae officinari Rhizoma extract decreased paw volume induced by plantar injection of carrageenan. Also Alpiniae officinari Rhizoma extract inhibited nitric oxide, prostaglandin E₂production and induced nitric oxide synthase, cyclooxygenase-2 protein expression in Mouse macrophage RAW 264.7 cells stimulated with lipopolysaccharide. Conclusion : This study shows that Alpiniae officinari Rhizoma extract seems to have anti-inflammatory effect by inhibition of nitric oxide, prostaglandin E_(2) production and nitric oxide synthase, cyclooxygenase-2 protein expression.

경연 대회를 통한 초ㆍ중고생의 창의력 신장 효과에 관한 연구

이희복,육근철,류해일,김현섭,김희수,박달원,유병환,김선효,김여상,서광수,변두원,서명석,배성효,박종석,심규철,이성희 공주대학교 과학교육연구소 2001 과학교육연구 Vol.32 No.1

미래 사회를 대비하기 위한 개인의 개성을 존중과 창의적인 능력을 지닌 인간을 양성을 목적으로 충청남도 지역의 초 ㆍ 중고등학생들을 대상으로 한 창의력 경연대회가 공주대학교 과학교육연구소에서 개최되었다. 충청남도 초 ㆍ중 ㆍ 고등학생들을 대상으로 실시한 창의력 경연대회는 창조적 아이디어를 내어 스스로 문제를 해결해 나갈 수 있는 교육 프로그램을 제공하는 좋은 기회였음이 확인되었으나, 일부의 팀에서만 참신하고 재미있는 아이디어들이 제안되었다. 여학생의 참가자 수가 상당히 증가하였으며, 대체적으로 입상권의 학생들의 상당 부분이 여학생이라는 것이 특이할만하다. 학년이 올라감에 따라서 창의적인 아이디어를 내는 양이나 질에 있어서 뒤떨어지는 것으로 분석되었으며, 토론 학습의 적응력이 미흡하고 발표력 및 청취력에 있어서도 떨어지는 것으로 나타났다. 따라서 중 ㆍ고등학교의 학교교육에서도 학생들에게 프로젝트형 탐구학습 프로그램의 적용은 물론 토론식 수습을 통한 학생들의 사고의 전환의 기회를 제공해야 할 것으로 사료된다. The creativity competition was held to foster the creativity of elementary and secondary school students in the Institute of Science Education, Kongju national University. It was found that the creativity competition effected on the improvement of inventing creative ideas and problem solving activities. But, only some competitors participated in semifinal and final contests, presented original and interesting ideas. More female students participated in competition than last year. More female participants were awarded a prize than males. Totally, participants were defective in discussion and communication, and presentation and listening ideas. Increasing grades correlated inversely with creativity and originality. There is need of inquiry project teaming programs and presenting opportunities of conversion of thinking by discussing instructions in school

계지의 항염 효과에 관한 연구

박히준,이지숙,이재동,김남재,표지희,강전모,최일환,김수영,심범상,이제헌,임사비나 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objectives : Cinnamomi Ramulus (CR), the young twing of Cinnamomi loureirii nees, has been used for treating symptoms related to pain. rheumatic arthritis and inflammation in Korean herb medicine. This study was carried out to investigate the anti-inflammatory effect of CR in vivo and in vitro. Methods : Extracts of CR were prepared and the chemical components of the extracts were examined by gas chromatography-mass spectrometry (GC-MS). The extracts were administrated to the rat paw edema model induced by carrageenan to evaluate the anti-inflammatory effect of CR. The expressions of nitric oxide (NO). prostaglandin E2(PGE2), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 were also quantified in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages to survey the effect of CR in vitro. The main components were cinnamaldechyde and coumarin. Results : We examined the anti-inflammatory activity of the 80% ethanol extract of Cinnamomi Ramulus in vivo by using carrageenan-induced rat paw edema model. Maximum inhibition of 54.9% was noted at the dose of 1000㎎/㎏ after 2 hours of drug administration in carrageenan-induced rat paw edema and this showed a potent anti-inflammatory effect. Conclusions : The results showed that Cinnamomi Ramulus suppressed dose-dependently LPS-induced NO production in RAW 264.7 macrophages and also decreased iNOS protein expression. Cinnamomi Ramulus also showed a significant inhibitory effect in LPS-induced PGE2 production and COX-2 expression.

Association between increment of serum VEGF level and prognosis after transcatheter arterial chemoembolization in hepatocellular carcinoma patients.

Shim, Ju Hyun,Park, Joong-Won,Kim, Ji Hoon,An, Min,Kong, Sun-Young,Nam, Byung-Ho,Choi, Joon-Il,Kim, Hyun Beom,Lee, Woo Jin,Kim, Chang-Min Japanese Cancer Association 2008 CANCER SCIENCE Vol.99 No.10

<P>We prospectively investigated the association between a change of serum vascular endothelial growth factor (VEGF) level after transcatheter arterial chemoembolization (TACE) and hepatocellular carcinoma (HCC) patient prognosis. The study involved 147 patients with unresectable HCC treated at the National Cancer Center, Korea, between July and December 2005. Serum samples were collected from each patient before TACE, and 1-2 days and 1 month after TACE. Serum VEGF concentrations were measured using an enzyme-linked immunosorbent assay (ELISA). The log(e)(VEGF/platelets) increased transiently 1-2 days after TACE and declined thereafter. Frequency of previous TACE did not correlate with log(e)(VEGF/platelets). This study found that log(e)(VEGF/platelets) 1-2 days after TACE, but not log(e)(VEGF/platelets) at baseline, was strongly correlated with vascular or nodal invasion and AJCC (American Joint Committee on Cancer)/UICC (International Union Against Cancer) stage, and was significantly greater in men. Relative changes in serum VEGF/platelet levels 1-2 days after TACE (DeltaVEGF) > 0.5 were directly correlated with tumor size, vascular invasion and modified UICC and AJCC/UICC stage (P < 0.05 for each). Additionally, DeltaVEGF > 0.5 was significantly correlated with newly developed extrahepatic metastases one and six months after TACE (P = 0.005 and 0.003, respectively). Progression free survival of patients with DeltaVEGF > 0.5 was significantly worse (P < 0.001) and DeltaVEGF > 0.5 was an independent prognostic factor for PFS (hazard ratio, 3.111; P < 0.001). This study showed that a high increment in serum VEGF level 1-2 days after TACE in HCC patients was associated with distant metastasis and unfavorable outcomes.</P>

일반긴장이론(GST)을 이용한 중학생의 휴대폰 의존에 미치는 영향

심현진(Hyun-Jin Shim),이일현(Il-Hyun Lee),이현실(Hyun-Sill Rhee) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.2

본 연구는 Agnew의 GST에 기반하여 중학생의 휴대폰 의존에 미치는 영향을 알아보려 하였다. 연구대상은 한국아동청소년패널 2010 중1 패널 1차년도 데이터를 이용하여 총 2,151명을 최종 연구에 이용되었다. 연구의 분석방법은 AMOS 21 Ver.를 사용하여 구조방정식으로 검증하였다. 본 연구의 결과는 다음과 같다. 첫째, 최종모형의 적합도 지수는 X2=1398.997, df=144, GFI=.937, NNFI(TLI)=.902, CFI=.918, RMSEA=.061으로 나타나 GST이론을 통해 중학생의 휴대폰 의존에 대해 검증하는데 타당한 것으로 나타났다. 둘째, 중학생의 긴장요인이 부정적 삶의 만족도를 형성하는데 통계학적으로 유의한 정(+)의 영향을 나타내는 것으로 나타났다. 마지막으로, 중학생의 부정적 삶의 만족도는 휴대폰 의존에 통계학적으로 유의 한 정(+)의 영향을 미치는 것으로 나타났다. 본 연구는 중학생의 휴대폰 의존에 관한 프로그램 개발, 중재적 방안을 모색하는데 기초적 자료로 제공하려 한다. The purpose of this study is to find out the effect of using cellular phone dependence of middle school student based on Agnew’s GST. A total of 2,151 questionnaires were used to Korean Children and Youth Panel Survey(KCYPS)2010. Analytical methods of Structural Equation Model(SEM) was verified by AMOS 21.0 Ver. The results were the following. Firstly, The fit of definitive model was X2=1398.997, df=144, GFI=.937, NNFI(TLI)=.902, CFI=.918, RMSEA=.061. Model was proper to verify the use of cellular phone dependence of adolescents. Secondly, The strain factors of middle school student presented statistically significant positive effect to make negative life satisfaction. Finally, The negative life satisfaction of middle school student presented statistically significant positive effect to the use of cellular phone dependence of middle school student. We need to provide as basic data for developing cellular phone dependence intervention programs.

LPG 자동차의 엔진 전자제어 시스템에 관한 컴퓨터의 고장사례 연구

이일권(Il Kwon Lee),국창호(Chang Hoo Kook),김청균(Chung Kyun Kim),김도현(Do Hyun Kim),심종현(Jong Hyun Shim),김영규(Young Gyu Kim),조승현(Seung Hyun Cho),문학훈(Hak Hoon Moon) 한국가스학회 2009 한국가스학회 학술대회논문집 Vol.2009 No.10

Pharmacokinetics and Efficacy of a Biweekly Dosage Formulation of Exenatide in Zucker Diabetic Fatty (ZDF) Rats

Kwak, Hyun-Hee,Shim, Won-Sik,Hwang, Seongmee,Son, Mi-Kyung,Kim, Yoon-Ji,Kim, Tae-Hyoung,Yoon, Zee-Hye,Youn, Hyun-Jun,Lee, Ghun-Il,Kang, Soo-Hyoung,Shim, Chang-Koo Springer-Verlag 2009 Pharmaceutical research Vol.26 No.11