Impact of selected fungi from an artificial diet on the growth and development of Drosophila suzukii (Diptera: Drosophilidae)

Huan-Huan Gao,Nan Xu,Hao Chen,Qian Liu,Qian-Yun Pu,Dong-Yun Qin,Yi-Fan Zhai,Yi Yu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Drosophila suzukii (Diptera: Drosophilidae) is one of the very few Drosophila species which are able to lay eggs and feed on healthy ripening fruit. Adults and larvae are all able to obtain the nutrition in the decaying food, which will generate many microorganisms. However, the relationship between D. suzukii and microorganism is poorly understood. In this study, 13 species of fungi were identified in an artificial diet fed by D. suzukii. Short-term and continuously life table experiments were conducted to determine the impact of three of these fungus species including Geotrichum candidum, Talaromyces minioluteus and Actinomucor elegans on the growth and development of Drosophila suzukii. Results revealed that, compared to the control, G. candidum, T.minioluteus and A. elegans increased the mortality of D. suzukii adults in the short time, while extended the developmental time of pupal D. suzukii by 18.00%, 16.22% and 26.44%, respectively in the life table experiment. T. minioluteus reduced the total longevity of D. suzukii by 15.52%, while A. elegans enhanced the total longevity by 25.96%. G. candidum and A. elegans increased the fecundity bymore than two folds. T. minioluteus elongated the mean generation time (T) by 31.34%, whereas G. candidum shortened it by 15.26% but increased the net reproductive rate (R0) by 217.76%, intrinsic rate of increase (r) by 88.89% and finite rate of increase (λ) by 9.17%. It was concluded that G. candidumand A. elegans significantly had the beneficial effect on the growth and reproduction of D. suzukii, however, T. minioluteus had the negative effect on D. suzukii. Our results could provide a new integrated pest management strategy for D. suzukii which would be discussed in this study.

Effect of Monascus sp. as an Adjunct Starter on Physicochemical Properties and Proteolysis in Semi-hard Cheeses during Ripening

Huaning Yu,Zhenmin Liu,Feng Hang,Beihong Mo 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

Two kinds of semi-hard cheeses, with Monascus purpureus and without M. purpureus, were manufactured, and effects of M. purpureus on physicochemical properties and proteolysis were evaluated during 36 days of ripening. Addition of M. purpureus changed the microbial survival and showed no significant effect on physicochemical properties of the cheeses, including dry matter and pH. Regardless on the rind or in the core, the indices of proteolysis had no significant difference (p>0.05), whereas there were significant differences of total free amino acid (FAA) and individual FAA between cheeses; this indicated that M. purpureus had no significant effect on the primary proteolysis, but affected the content and ratio of individual FAAs during maturation. Electrophoretic analysis showed strong degradation of αs1-casein in the core and on the rind of cheeses, while β-casein was highly degraded on the rind but less in the core. Thus, Monascus spp. might have a potential application in the manufacture of cheeses.

Research on the Application of Big Data Ecology in College Physical Education Teaching and Training

Yu Huan,Choo Jongho,Song. Jeho 한국스포츠학회 2020 한국스포츠학회지 Vol.18 No.4

빅데이터의 발달과 진보가 대학생들의 스포츠 학습에 새로운 힘을 실어주고 있다. 게다가 방대한 정보의 희소성, 중복성, 모호성은 빅데이터를 대학 스포츠에 접목시키는 데 어려움을 초래한다. 이 글에서는 스포츠 데이터베이스를 만드 는 방법, 응용 데이터 분석을 대학 스포츠 학습 및 훈련에 통합하는 방법, 빅데이터 방법을 통한 교육 개혁 방법에 대해 논의하였다. 이러한 데이터 정보의 실질적인 적용은 대학생들에게 이익이 될 것이다. 빅데이터 분석에서 데이터 유출과 개인정보 유출이 발생하면 그 결과가 매우 심각하다. 교사들은 이것에 큰 중요성을 부여하고 이러한 상황을 피하기 위해 몇 가지 조치를 취하고자 한다. The development and progress of big data has given new support to college students' sports learning. Moreover, the scarcity, redundancy and ambiguity of massive information bring us difficulties in integrating big data into university sports. This article discusses how to create a sports database, how to integrate applied data analysis into college sports learning and training, and how to reform education through big data methods. Practical application of these data information will benefit college students. When data leakage and personal information leakage occur in big data analysis, the consequences are very serious. Teachers attach great importance to this and take some measures to avoid this situation.

Green Tea Consumption May Be Associated with Cardiovascular Disease Risk and Nonalcoholic Fatty Liver Disease in Type 2 Diabetics: A Cross-Sectional Study in Southeast China

Huan-Huan Yang,Hui Zhou,Wan-Zhan Zhu,Cai-Long Chen,Guo-Chong Chen,Lu-Gang Yu,Li-qiang Qin 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.10

Dietary factors play a crucial role in the management of type 2 diabetes mellitus (T2DM) by reducing cardiovascular disease (CVD) risk. Therefore, we aimed to examine the associations between habitual green tea consumption and risk factors of CVD among T2DM patients. A total of 1013 patients with T2DM were included in a community-based cross-sectional study. Data on dietary habits, including tea consumption, were collected using a food frequency questionnaire. A multivariable logistic regression model was used to analyze the associations. In men, as compared with nongreen tea drinkers, odds ratios (ORs) (95% confidence interval [CI]) of nonalcoholic fatty liver disease (NAFLD) were 2.06 (95% CI, 1.20–3.55) for those with green tea consumption of once per day and 2.45 (95% CI, 1.31–4.58) for more than or equal to twice per day (P-trend = .004); ORs (95% CI) of general obesity were 2.19 (95% CI, 1.02–4.68) and 2.70 (95% CI, 1.18–6.21), respectively (P-trend = .021); whereas no such association was found in women. Sensitivity analysis according to self-awareness of their T2DM status revealed that the positive association between green tea consumption and general obesity was not reliable. Higher intake of green tea was still positively associated with NAFLD, but it only persisted in participants aged ≥52 years or the lower dietary quality subgroup in further analyses. Our findings suggest that tea consumption was associated with an increased risk of NAFLD among male T2DM patients aged 52 years or older, and those with lower dietary quality, which needs to be confirmed in future prospective studies.

Feeding and related morphology of salivary glands of Bradysia odoriphaga (Diptera: Sciaridae)

Huan-Huan Gao,Yi-Fan Zhai,Xue Cao,Xian-Hong Zhou,Zhong-Yan Wang,Fang Wang,Hao Chen,Yi Yu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2

The amount of Bradysia odoriphaga larval feeding at each developmental stage and the related morphology of salivary glands were investigated in this study. The amount of daily feeding in the third instar was greatest among all the developmental stageswhile the amount of cumulative feeding in the fourth-instar accounted for 53.56% of the total amount of larval feeding. The daily amount of feeding by B. odoriphaga during the whole larval stage peaked in the 8th and 14th day after hatching. The paired and transparent salivary glands of B. odoriphaga were characterized by two distinct regions: an anterior region and a posterior region. At the fourth-instar and pupal stage, the length of salivary glands decreased significantly compared with that of the third-instar, and the integrity of epithelium cytoplasm in the salivary glands also decreased markedly because of the formation of cytoplasmic vacuoles and degradation of rough endoplasmic reticulum and mitochondria. We concluded that the decrease of daily feeding amount in the last instar was related to the structural change of salivary gland closely. The function of salivary glands in B. odoriphaga was mainly secretion of silk and cocooning-like materials during the last larval instar.

The Effect to Alleviate Pressure Sores by Installing Nasal Continuous Positive Airways

Huan yu Hsu,Pi chi Lin,Ming Shan Hsieh 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

Impact of fatty liver on long-term outcomes in chronic hepatitis B: a systematic review and matched analysis of individual patient data meta-analysis

Yu Jun Wong,Vy H. Nguyen,Hwai-I Yang,Jie Li,Michael Huan Le,Wan-Jung Wu,Nicole Xinrong Han,Khi Yung Fong,Elizabeth Chen,Connie Wong,Fajuan Rui,Xiaoming Xu,Qi Xue,Xin Yu Hu,Wei Qiang Leow,George Boon-B 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.3

Background/Aims: Chronic hepatitis B (CHB) and fatty liver (FL) often co-exist, but natural history data of this dual condition (CHB-FL) are sparse. Via a systematic review, conventional meta-analysis (MA) and individual patient-level data MA (IPDMA), we compared liver-related outcomes and mortality between CHB-FL and CHB-no FL patients. Methods: We searched 4 databases from inception to December 2021 and pooled study-level estimates using a random- effects model for conventional MA. For IPDMA, we evaluated outcomes after balancing the two study groups with inverse probability treatment weighting (IPTW) on age, sex, cirrhosis, diabetes, ALT, HBeAg, HBV DNA, and antiviral treatment. Results: We screened 2,157 articles and included 19 eligible studies (17,955 patients: 11,908 CHB-no FL; 6,047 CHB-FL) in conventional MA, which found severe heterogeneity (I2=88–95%) and no significant differences in HCC, cirrhosis, mortality, or HBsAg seroclearance incidence (P=0.27–0.93). IPDMA included 13,262 patients: 8,625 CHB-no FL and 4,637 CHB-FL patients who differed in several characteristics. The IPTW cohort included 6,955 CHB-no FL and 3,346 CHB-FL well-matched patients. CHB-FL patients (vs. CHB-no FL) had significantly lower HCC, cirrhosis, mortality and higher HBsAg seroclearance incidence (all P≤0.002), with consistent results in subgroups. CHB-FL diagnosed by liver biopsy had a higher 10-year cumulative HCC incidence than CHB-FL diagnosed with non-invasive methods (63.6% vs. 4.3%, P<0.0001). Conclusions: IPDMA data with well-matched CHB patient groups showed that FL (vs. no FL) was associated with significantly lower HCC, cirrhosis, and mortality risk and higher HBsAg seroclearance probability.

Cytotoxic Effect of Oyster Mushroom Pleurotus ostreatus on Human Androgen-Independent Prostate Cancer PC-3 Cells

Yu-Huan Gu,Gowsala Sivam 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.2

Twenty species of edible mushrooms and three purified mushroom polysaccharides were screened for their an-titumor potential on human androgen-independent cancer PC-3 cells. A water-soluble extract (POE) prepared from the freshoyster mushroom Pleurotus ostreatusproduced the most significant cytotoxicity on PC-3 cells among the mushroom speciestested. At the same time, POE induced a rapid apoptosis on PC-3 cells detected with annexin V-fluorescein isothiocyanateflow cytometry when the cells were exposed to POE (150 .g/mL) for 2 hours. Induced apoptosis was also confirmed by DNAfragment terminal deoxynucleotidyl transferase-mediated X-dUTP nick end labeling staining while POE (200 .g/mL) wasadded to PC-3 cells for 6 hours. Both cytotoxicity and induced apoptosis mediated by POE in PC-3 cells are dose-dependent.Interestingly, PC-3 cells appeared to be more sensitive to POE in anchorage-independent growth condition. Tumor colony-forming efficiency was dramatically reduced to 4.5% or 0.5% in POE (60 or 120 .g/mL)-supplemented soft agar mediumcompared with that of POE-free medium (defined as 100%). Temperature in POE processing plays a decisive role for the cy-totoxic activity. Bioactivity of POE was eliminated by exposure to high temperature (80°C) for 2 hours; however, it remained

Preparation and molecular characterization of a polyclonal antibody as an efficient cutworm reference protein

Huan Yu,Lei Hea,Yi-Yi Ou-Yang,Guo-Hua Huang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3

Antibodies, which are wildly used in molecular researches, are proteins secreted by plasma cells that can specifically recognize specific antigens. To obtain a stable reference antibodies are important for protein analysis. In this study, a capable glyceraldehyde phosphate dehydrogenase (GAPDH) antiserum against most noctuid larval protein samples was prepared. Firstly, the full-length gapdh was amplified from Spodoptera exigua larvae to construct the prokaryotic expression vector. The purified His-tag fused GAPDH protein was used to immunize rabbits for the antiserum preparation. Protein samples extracted from 11 insect species distributed across seven families and three orders were used to perform the Western bolt analysis. The bright specific bands were detected in S. exigua, S. litura, Helicoverpa armigera, and Mythimna seperata protein samples, indicating that this antiserum may be capable of detecting noctuid larval encoded GAPDH. Further immunofluorescence identification of H. armigera and S. exigua paraffin section slides with GAPDH antiserum exhibited an identical cytostained image. The GAPDH antiserum prepared in this study is useful and efficient as reference antibodies in studies involving noctuid larval protein samples in other related fields.

First-Principles Study of the Influence of Zirconium on the Diffusion of Uranium Defects in Uranium Dioxide

Huan Wang,Yu Zou,Zhong Zhang,Kai-Yuan Wang,Huan An,Chang-Yong Zhan,Jun Wang,Jian-Chun Wu 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.74 No.8

The migration properties of uranium vacancies and interstitials in zirconium-doped uranium dioxide are studied by using density functional theory (DFT) and the climbing-image nudge elastic band (CI-NEB) method. The strong correlations among uranium $5f$ electrons were described by using a spherically averaged Hubbard parameter. In the model, the zirconium atoms are introduced by replacing the uranium atoms at the nearest and the next nearest neighbor sites along the diffusion path of uranium defects. The doping with zirconium obviously reduces the migration barriers for defects in uranium dioxide. The effect of doping with zirconium on the diffusion of uranium defects decreases with increasing distance between the zirconium dopant and the uranium defects. Further, we investigated the lattice distortion and the electron transfer associated with the migration of uranium defects, and we analyzed the physical origin of the reduction in the migration barriers caused by zirconium doping.