Characterization of a novel variant HMW‐glutenin gene from Elymus canadensis

Qian‐Tao Jiang,Yu‐Ming Wei,Tao Liu,Ji‐Rui Wang,Zhi‐En Pu,Xiu‐Jin Lan,You‐Liang Zheng,Zhen‐Xiang Lu 한국유전학회 2010 Genes & Genomics Vol.32 No.4

High molecular weight (HMW) glutenin subunits (GS) play a key role in the determination of end‐use quality of wheat and other cereal crops. In this study, we report the isolation and characterization of both promoter region and ORF of novel HMW‐GS allele 1St1.3 from a perennial Triticeae species,Elymus canadensis. The amino acid (AA) sequences of E. canadensis 1St1.3 were deduced as 434 aa. Its protein primary structure comprises a signal peptide with a conserved N‐terminal domain, a central repetitive domain and a C‐terminal domain. E. canadensis 1St 1.3 possesses several distinct characteristics which are different from those of wheat HMW‐GSs. The N‐terminal domains of E. canadensis 1St 1.3 resemble that of y‐type subunits, while their C‐terminal domains are more similar to x‐type subunits. The deletion of 85 bp fragment has been observed in promoter region of 1St 1.3, however which has not interrupted the expression of this gene. Our results indicate that 1St 1.3 is novel HMW‐GS variants which will be valuable for enhancing our understanding of structural differentiation and the evolutionary relationship among HMW‐GSs in Triticeae species.

Hypoxia Mediates Runt-Related Transcription Factor 2 Expression via Induction of Vascular Endothelial Growth Factor in Periodontal Ligament Stem Cells

Manjing Deng,Qian Xu,Zhihua Liu1,Ling Guo,Rui Liu,Rulei Li,Xiang Chu,Jiajia Yang,Jia Luo,Faming Chen 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11

Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible factor-1α (HIF-1α) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of HIF-1α, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of HIF-1α, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride (CoCl2, 100 μmol/L), an agonist of HIF-1α, and suppressed by 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10 μmol/L), an antagonist of HIF-1α. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF (hVEGF165) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via HIF-1α-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs

Highly Efficient Shoot Regeneration from Cotyledonary Nodes of Vegetable Soybean

Qian-Qian Liu,Gang Chen,Jun-Yi Gai,Yue-Lin Zhu,Li-Fei Yang,Guo-Ping Wei,Cong Wang 한국원예학회 2010 원예과학기술지 Vol.28 No.2

풋콩(Glycine max (L.) Merrill) 6개 품종의 자엽절 절편체로부터 thidiazuron(TDZ)과 NAA 농도를 달리한 배지에서 신초의 효율적인 재분화를 조사한 후, 가장 효과가 좋은 생장조절물질 조합으로 배지 종류, 접종방법 및 품종별 신초 재분화율을 조사하였다. 또한 절편체를 배지에 수직 또는 수평으로 치상하는 방법과 B5, 1/2 B5, MS, 1/2 MS, MSB(MS salts + B5 organics) 등의 5가지 기본 배지에 관하여 시험하였다. B5 배지에 1㎎?L<SUP>-1</SUP> TDZ, 0.05㎎?L<SUP>-1</SUP> NAA, 5㎎?L<SUP>-1</SUP> AgNO₃를 첨가한 처리에서 6개 품종의 신초 재분화율이 55.3-88.9%로 높았다. ‘L?ling No. 1’의 경우 수직으로 치상하는 것이 수평으로 치상하는 것보다 신초 재분화율이 37.5% 더 높았다. 따라서 풋콩은 TDZ를 처리한 B5 배지에 자엽절을 수직으로 치상할 때 신초 재분화율이 높았다. To establish a highly efficient system for shoot regeneration in vegetable soybean (Glycine max (L.) Merrill), explants were obtained from six genotypes and adventitious shoots were regenerated from cotyledonary nodes cultured on medium supplemented with different concentrations of N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (thidiazuron TDZ) and 1-naphthylacetic acid (NAA). The most effective combination of plant growth regulators was selected first and then the effects of medium types, inoculation methods and genotypic differences on shoot regeneration were studied. The explants were inserted either vertically or horizontally into the medium and five basal media, including B5, ½ B5, MS, ½ MS, and MSB (MS salts and B5 organics), were tested. The shoot regeneration frequency of the six genotypes ranged from 53.5% to 88.9% and three of them reached 88.9%, 87.5% and 83.3%, respectively, on B5 medium supplemented with 1 ㎎?L<SUP>-1</SUP> TDZ, 0.05 ㎎?L<SUP>-1</SUP> NAA, and 5 ㎎?L<SUP>-1</SUP> AgNO₃. The shoot regeneration frequency of explants cultured on B5 medium was significantly higher than that of the other four basal media. The vertically inserted explants were found to yield a higher shoot regeneration frequency than that of horizontally inserted ones; the maximum difference of regenerating percentages between the two methods was 37.5%. In summary, TDZ was an efficient plant growth regulator for shoot induction. B5 medium and vertically inserted explants promoted shoot regeneration. We believe this highly efficient shoot regeneration system will provide foundation for the further transgenic studies in vegetable soybean.

The ERCC1 C118T Polymorphism Predicts Clinical Outcomes of Colorectal Cancer Patients Receiving Oxaliplatin-Based Chemotherapy: a Meta-analysis Based on 22 Studies

Qian, Ying-Ying,Liu, Xin-You,Wu, Qian,Song, Xian,Chen, Xiao-Feng,Liu, Yi-Qian,Pei, Dong,Shen, Li-Zong,Shu, Yong-Qian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: Although the predictive value of the excision repair cross-complementing group 1 (ERCC1) C118T polymorphism in clinical outcomes of patients with colorectal cancer (CRC) receiving oxaliplatin-based chemotherapy has been evaluated in numerous published studies, the conclusions are conflicting. Therefore, we performed the present meta-analysis to determine the precise role of the ERCC1 C118T polymorphism in this clinical situation and help optimize individual chemotherapy. Materials and Methods: A multiple search strategy was used to identify eligible studies. Pooled odds ratios (ORs) and their 95% confidence intervals (CIs) were used to estimate objective response and oxaliplatin-induced toxicity, with hazard ratios (HRs) with 95%CIs for progression-free survival (PFS) and overall survival (OS). Results: A total of 22 studies including 2,846 CRC patients were eligible in the analysis. Overall, no significant correlation was found between the ERCC1 C118T polymorphism and objective response to oxaliplatin-based chemotherapy, in all patients or in the Asian and Caucasian subgroups. However, the pooled analysis showed that the PFS and OS were significantly shorter in patients who carried T/T or T/C genotypes of ERCC1 C118T as compared to the C/C genotype. On stratified analysis by ethnicity, the ERCC1 118T allele was associated with a favorable prognosis in Caucasians (PFS, HR=0.58, 95%CI: 0.24-1.44; OS, HR=0.38, 95%CI: 0.22-0.64) but an unfavorable prognosis in Asians (PFS, HR=2.49, 95%CI: 1.87-3.33; OS, HR=2.63, 95%CI: 1.87-3.69) based on a dominant model. In addition, we failed to find a statistically significant impact of ERCC1 C118T polymorphism on oxaliplatin-induced toxicity. Conclusions: The ERCC1 C118T polymorphism may have prognostic value in patients with CRC undergoing oxaliplatin-based chemotherapy.

The XPD Lys751Gln Polymorphism has Predictive Value in Colorectal Cancer Patients Receiving Oxaliplatin-Based Chemotherapy: a Systemic Review and Meta-analysis

Qian, Ying-Ying,Liu, Xin-You,Pei, Dong,Xu, Jia-Li,Shen, Hua,Chen, Xiao-Feng,Liu, Yi-Qian,Shen, Li-Zong,Shu, Yong-Qian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: The predictive value of the xeroderma pigmentosum group D (XPD) Lys751Gln polymorphism regarding clinical outcomes of patients with colorectal cancer (CRC) receiving oxaliplatin-based chemotherapy has been evaluated in numerous published studies, but the results remain inconclusive. Therefore, we performed a meta-analysis to determine the precise role of the XPD Lys751Gln polymorphism in this clinical situation and optimize individual chemotherapy. Materials and Methods: A multiple search strategy was used to identify eligible studies. Pooled odds ratios (ORs), generalized odds ratio (ORG) and their 95% confidence intervals (CIs) were used to estimate the objective response, while hazard ratios (HRs) with 95%CIs were used for progression-free survival (PFS) and overall survival (OS). Results: A total of 17 studies including 2,286 patients met the inclusion criteria. Overall, the XPD 751Gln allele was associated with a non-significant reduced objective response to oxaliplatin-based chemotherapy in all patients or in the Asian and Caucasian subgroups. However, poor PFS and OS of CRC patients treated with oxaliplatin-based regimens were significantly related to the XPD 751Gln allele in the dominant model (PFS: HR=2.10, 95%CI: 1.65-2.67; OS: HR=3.18, 95%CI: 1.57-6.47). On stratified analysis by ethnicity, these relationships were more pronounced in Asians (PFS: HR=2.49, 95%CI: 1.79-3.47; OS: HR=5.25, 95%CI: 3.46-7.94) than in Caucasians (PFS: HR=1.73, 95%CI: 1.22-2.46; OS: HR=1.78, 95%CI: 1.06-2.99). Conclusions: The XPD Lys751Gln polymorphism may have prognostic value in patients with CRC undergoing oxaliplatin-based chemotherapy.

High Efficient in Vitro Shoot Regeneration from Cotyledonary Nodes of Vegetable Soybean

Qian-Qian Liu,Jun-Yi Gai,Yue-Lin Zhu,Gang Chen,Li-Fei Yang,Guo-Ping Wei,Cong Wang 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Quaternion Discrete Tchebichef Moments and Their Applications

Hongqing Zhu,Qian Li,Qian Liu 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.6

The concept of the quaternion is useful for colour image processing and recognition. This paper introduces quaternion discrete Tchebichef moments (QTM), which use the traditional Tchebichef moments (TM) of each colour channel to describe colour images. A set of invariants that are invariant to translation and scale transformations is introduced for colour object recognition and image classification. A theoretical framework is provided for the recognition of colour face images by combining the proposed quaternion Tchebichef moment functions with the sparse representation classification (SRC) strategy for improving recognition despite partial occlusions. Simulation results on standard colour face databases demonstrate the effectiveness of the proposed algorithm, even when the images include Gaussian or pepper-and-salt noise.

Liquid/Liquid Interfacial Fabrication of Thermosensitive and Catalytically Active Ag Nanoparticle-Doped Block Copolymer Composite Foam Films

Liu, Mei,Wang, Qian,Geng, Yuanyuan,Wang, Changwei,Lee, Yong-Ill,Hao, Jingcheng,Liu, Hong-Guo American Chemical Society 2014 Langmuir Vol.30 No.34

<P>An aqueous solution of AgNO<SUB>3</SUB> (upper phase) and a DMF/CHCl<SUB>3</SUB> solution of polystyrene-<I>b</I>-poly(acryl acid)-<I>b</I>-polystyrene (PS-<I>b</I>-PAA-<I>b</I>-PS) or PS-<I>b</I>-PAA-<I>b</I>-PS/1,6-diaminohexane (DAH) (lower phase) constituted a planar liquid/liquid interface. The lower phase gradually transformed to a water-in-oil (W/O) emulsion via spontaneous emulsification due to the “ouzo effect”. Polymer molecules, DAH molecules, and Ag<SUP>+</SUP> ions assembled into microcapsules around emulsion droplets that adsorbed at the planar liquid/liquid interface, resulting in formation of a foam film. DAH acted as a cross-linker during this process. Transmission electron microscopic observations indicated that Ag nanoclusters that were generated through reduction of Ag<SUP>+</SUP> ions by DMF were homogeneously dispersed in the walls of the foam structure. X-ray photoelectron spectroscopic investigations revealed that Ag(I) and Ag(0) coexisted in the film, and Ag(I) transformed to Ag(0) after further treatment. The film formed without DAH was not stable, while the film formed with DAH was very stable due to intermolecular attraction between PAA and DAH and formation of amides, as revealed by FTIR spectra. The film formed with DAH exhibited high and durable catalytic activity for hydrogenation of nitro compounds and, very interestingly, exhibited thermoresponsive catalytic behavior.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2014/langd5.2014.30.issue-34/la502738j/production/images/medium/la-2014-02738j_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/la502738j'>ACS Electronic Supporting Info</A></P>

[BOOK REVIEW] China’s leftover women: Late marriage among professional women and its consequences

Qian LIU 이화여자대학교 아시아여성학센터 2015 Asian Journal of Women's Studies(AJWS) Vol.21 No.4

Improving the Self-adaptive Rate Control to Wireless Multicast for Virtual Environment

Liu Li,Chen Hong-qian,ChenYi,YaoChun-Lian 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.11

Distributed virtual environment requires high stability and low delay to support real-time data transmission for large number of users. In wireless network-based virtual environment, traditional transmission protocol fails to meet the requirements of virtual environment due to variety of nodes distribution, complexity, and difference of bandwidth. Based on our research of rate control for distributed virtual environment, we hereby propose a bandwidth self-adaptive rate control algorithm based on layers of interest. According to effective bandwidth of proxy gateway in distributed virtual environment, this method solving the problem of time inconsistency between publishers and subscribers, constraining occupied bandwidth within the range of effective bandwidth of gateways. Our experiments prove that this algorithm can solve the problem that users’needs of interactive with each other very frequently in wireless network virtual environment. The algorithm automatically match data transmission based on bandwidth and the received datagram is smooth and much more stable. The prime performances meet our expected research objectives.