서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Surface Plasmon Resonance 기술을 이용한 인체분비물 혈액형 분석에 대한 연구

홍승범,최동호,안희중,이동석,김응수,최상규,이영보 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

CMOS 집적회로의 테스팅을 위한 새로운 내장형 전류감지 회로의 설계

홍승호,김정범 강원대학교 정보통신연구소 2004 정보통신논문지 Vol.8 No.-

This paper presents a Built-In Current Sensor that detects defects in CMOS integrated circuits using the current testing technique This scheme employs a cross-coupled connected PMOS transistors, it is used as a current comparator. Our proposed scheme has a negligible impact on the performance of the circuit under test (CUT). In addition, in the operation of the normal mode, the Built-In Current Sensor(B1CS) does not have dssipation of extra power, high speed detection tune and it can be appliel deep submcron process. The validty and effectiveness are verified through the HSPICE simulation on circuits with defects The area overhead of a BICS versus the entire chip is about 9.2%. The chip was fabricated with Hynix 0.35,m 2-poly 4-metal N-well CMOS technology.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

생물검정법을 이용한 하수슬러지 장기연용 토양의 독성평가

남재작,이승환,권순익,홍석영,임동규,고문환,송범헌 한국환경농학회 2004 한국환경농학회지 Vol.23 No.4

생물검정법에 의한 토양 내 독성평가의 유용성을 확인하기 위해 두 종류의 하수슬러지를 7년간 연용한 토양을 Microtox^(?) 생물검정법과 상추종자 뿌리 신장법을 사용하여 독성을 평가하였다. 이 방법들은 하수오니의 종류나 시용량에 따른 토양의 독성 정도를 잘 평가 할 수 있었다. 상추종자 뿌리 신장법은 특별한 장비나 복잡한 처리 없이도 유의성 있는 결과를 구할 수 있었고 Microtox^(?) 검정법은 전반적으로 재현성과 감도에서 유의성이 있었으며 또한 유기화합물에 대한 종합적인 평가도 가능하였다. 하수오니와 같이 다양한 화합물이 혼재되어 있는 유기성 자원의 토양 시용시 기존의 제한된 화학성분의 토양 분석법만으로는 독성평가에 한계가 있고 또 개별적으로는 별 영향이 없더라도 여러 화합물이 복합적으로 존재할 때 나타나는 독성작용에 대한 평가는 더욱이 어렵다. 그러므로 이러한 영향을 종합적으로 평가할 수 있는 생물검정법이 토양의 독성 평가에서 화학분석법을 보완하는 평가방법으로서의 중요성은 충분하다고 할 수 있다. 그리고 생물독성법의 유용성을 높이기 위해서는 생물검정법의 시료 추출방법 개선과 실험의 재현성을 높이기 위한 노력이 필요하리라 생각된다. 따라서 이런 생물검정법의 많은 제약에도 불구하고 환경의 종합적인 평가에 있어서 생물검정법의 중요성은 대단히 크며 직접적으로 생물을 이용하는 방법뿐만 아니라 유전독성 평가 방법 등 다양한 방법들이 환경독성 평가에 응용될 수 있을 것으로 판단된다. Bioassay was employed to assess toxicity of soil which had been treated with sewage sludges for seven years. The Microtox^(?) and root elongation test of lettuce (Lactuca Saliva) elucidated that the intensity of soil toxicity was closely related with the types and amount of sewage sludges applied. Both bioassay methods proved to be useful in an assessment of soil toxicity and were consistent to some extent with the conventional chemical analysis methods. EC_(50) values resulted from Microtox^(?) were highly correlated with concentration of heavy metals in soils amended with sewage sludges : Cu (r²= 0.86^(**)), Cr (r²= 0.84^(**)), Ni (r²= 0.83^(**)), and Zn (r²= 0.69^(**)). This demonstrated that both bioassay techniques could be employed as tools for soil toxicity assessment when the soil was exposed to solid wastes such as sewage sludge.

팥바구미 저항성 녹두의 작물학적 특성

이구환,정승근,박상일,송범헌,조용구,김홍식 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

급성 백혈병 환자에서 감염증의 양상 및 예후인자에 관한 연구 : 1988년∼1995년까지

정희진,김병수,신상원,김열홍,김우주,김민자,김준석,박승철,김권범 대한감염학회 1998 감염 Vol.30 No.3

목적 : 급성 백혈병의 경우 골수기능이 억제되고 항암화학요법에 따른 골수기능 저하로 감염의 위험이 증가하며 감염의 치료가 환자의 생존에 큰 영향을 미치게 된다. 이에 저자들은 1988년부터 1995년까지 고대 구로병원에 급성 백혈병으로 입원했던 환자들 중 입원 기간 내 감염이 있었던 경우를 대상으로 하여 감염의 위험인자 및 감염증의 예후를 후향적으로 분석함으로써 향후 급성 백혈병환자의 감염의 치료에 대한 지침을 얻고자 하였다. 방법 : 급성 백혈병으로 확진된 환자들 중 감염병에 이환된 경우를 대상으로 하여 각각을 미생물학적 확인감염, 임상적 확인감염, 그리고 설명 불가열로 구분하였다. 각각의 경우에서 감염 당시의 호중구 수, 호중구 감소 기간, 항암화학요법과의 연관성, 감염 병소, 배양된 검체 및 원인균, 선택적 소화관 살균법의 사용여부 및 골수회복 여부 등을 조사한 후 환자의 예후와 어떤 관계가 있는 지 조사하였다. 결과 : 연구 대상은 총 113예로 남녀비는 46:67 이었고 평균연령은 34±13세, 진단은 급성 골수성 백혈병 (AML)이 84예, 급성 림프구성 백혈병(ALL)이 29예였으며, 감염시 평균 호중구수는 663±1678/㎣이었고, 호중구 감소 기간은 평균 18±13일이었다. 감염양상은 항암화학요법과 연관된 감염이 84예로 대부분이었고 미생물학적 확인 감염이 40예로서 35%였으며, 폐렴과 원발성 패혈증이 각각 20예와 19예로 가장 많았고, 카테터 연관 감염이 7예로 나타났다. 배양된 균주는 E. coli가 10예(25%)로서 가장 많았으며, 전체적으로 E. coli, P. aeruginosa, K. pneumoniae 등의 그람음성균이 63%로 대부분을 차지하였으나 최근 그람양성구군 및 진균의 분리율이 증가하는 양상을 보였다. 감염증의 예후를 분석한 결과 진균 감염인 경우나 호중구 감소 기간이 긴 경우 사망률이 높았으며 그 중에서도 골수 기능이 회복 여부가 환자들의 생존과 통계적으로 유의한 연관이 있었다.(P=0.01). 결론 : 급성 백혈병 환자들에서의 감염은 관해유도화학요법 후 초래되는 호중구 감소와 유의한 관계가 있으며, 폐렴 및 패혈증의 임상양상을 보이는 경우가 흔하고, 원인균별로는 그람 음성균이 주류를 이루나 최근 그람 양성균 감염이 증가하는 추세를 보였다. 감염의 예후 인자로는 진균 감염인 경우나 호중구 감소 기간이 긴 경우 사망률이 높았으나 골수 기능의 회복여부가 결정적인 역할을 하는 것으로 나타나, 향후 급성 백혈병 환자의 치료에 있어 감염 빈도의 감소와 감염에서의 조속한 회복을 위해서는 골수 기능의 회복에 중점을 두어야 할 것으로 사료된다. Background : Infection is one of the most important and fatal complications in patients with acute leukemia. The characteristics of infection in acute leukemic patients are different from those in other diseases by the lack of normal inflammatory responses or distinct clinical evidence except fever. To improve the outcome of acute leukemia, it is very important to recognize the risk factors, patterns and prognostic factors of acute leukemia. Methods : We analyzed retrospectively the patterns of infection from 113 febrile patients with acute leukemia from January, 1988 to December, 1995. To determine the prognostic factors and the outcome of infection, the following variables were analyzed: the presence of neutropenia, use of chemotherapeutic agents, type and site of infections, isolated organisms, gastrointestinal decontamination, duration of neutropenia, and bone marrow recovery. Results : Out of 113 febrile patients with acute leukemia, 84 infection episodes(74%) occurred after chemotherapy. The mean duration of neutropenia was 18±13 days. The incidence of microbiologically-documented infection(MDI) was 35%(40/113). Pneumonia was the most common infection(26%), followed by primary sepsis(24%), catheter-related infection(9%). In cases of MDI, 63% were caused by gram-negative bacteria, followed by gram-positive bacteria(28%), and fungi(10%). Escherichia coli(25%) was the most common isolated in MDI. Regarding the prognostic factors in cases with infections, the recovery of bone marrow function was the only statistically significant factor(P=0.01). Conclusion : Infection has been a major cause of morbidity and mortality in acute in acute leukemic patients. To prevent infection and thereby improve the prognosis of acute leukemia, restoration of bone marrow function at early stage is important.

Research Articles : Isolation and Identification of Aspergillus Section Fumigati Strains from Arable Soil In Korga

( Seung Beom Hong ),( Dae Ho Kim ),( In Cheol Park ),( Robert A Samson ),( Hyeon Dong Shin ) 한국균학회 2010 Mycobiology Vol.38 No.1

Variation of Protein, Oil, Fatty acids, and Sugars Contents in Black Soybean Cultivars Grown at Different Latitudinal Locations

Seung-Beom Hong,Su-Jin Lee,Young-Hak Kim,Young-Sun Hwang,Kwang-Hee Yoon,Sung-In Lee,Mi-Young Nam,Lee-Seul Song,In-Youl Baek,Myoung-Gun Choung 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Crude protein is about 40% in soybean seed, and oil extracted from soybean seeds is one of the major vegetable oils. Also soybean oil is a good source of essential fatty acids, and sugars in soybean seed is composed of water-soluble saccharides, and most of them are oligosaccharide namely sucrose, raffinose, and stachyose. Although the production of soybean decreased in Korea, the consumption of soybean increased in recent time because soybean is well known as a well-being and healthy food. Therefore, this experiment was conducted to know the variation of crude protein, crude oil, fatty acids, and sugar contents in Korean domestic black soybean cultivars grown at different latitudinal locations, Suwon (37°16'N) and Milyang (35°30'N). In most black soybean cultivars, oleic acid content was higher, while linoleic acid, or linolenic acid contents were lower at higher latitudinal Suwon compared to lower latitudinal Milyang. Sucrose content in Geomjeongkong # 3, raffinose contents in Gemjeongkongkong # 3, # 4, Ilpumgeomjeongkong, and Cheongjakong, and stachyose content in Gemjeongkong # 1 grown at lower latitudinal Milyang was higher compared to higher latitudinal Suwon. The variations of crude protein, crude oil and fatty acids contents seemed to be affected by genotype than growing locations according to different latitude as they did not show the significant interaction between cultivars and locations. In contrast, the variations of glucose, sucrose, and stachyose contents maybe affected by environmental condition as different latitude than the genotype because they showed the significant interaction between cultivars and locations.

Variation of Anthocyanin, and Isoflavone Contents in Korean Black Soybean Cultivars Grown at Different Latitudinal Locations

Seung-Beom Hong,Su-Jin Lee,Young-Hak Kim,Young-Sun Hwang,Kwang-Hee Yoon,Sung-In Lee,Mi-Young Nam,Lee-Seul Song,In-Youl Baek,Myoung-Gun Choung 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Anthocyanin pigments in black soybean seed coat have been known as including biological activities, which are antitumor, antioxdant and antivirus. Also in soybean, isoflavone have been reported as health-promoting benefits, which are antiestrogen, antioxidant, antitumoral activities and preventing cardiovascular diseases and osteoporosis. The consumption of soybean increased in Korea because of the importance of its functional characteristics of the anthocyanin and isoflavone. Therefore, this experiment was conducted to investigate the variation of anthocyanin, and isoflavone contents in Korean domestic black soybean cultivars grown at different latitudinal locations, Suwon (37°16'N) and Milyang (35°30'N). D3G, C3G, and total anthocyanins in Geomjeongkong # 3, C3G, and total anthocyanins in Ilpumgeomjeongkong grown at higher latitudinal Suwon were higher compared to lower latitudinal Milyang. Daidzein, glycitein, genistein, and total isoflavone contents in Gemjeongkong # 4 were highest among the seven cultivars. Daidzein contents in Gemjeongkong # 3, Milyang # 112, and Milyang # 113 grown at higher latitudinal Suwon were higher compared to lower latitudinal Milyang. Glycitein contents in Gemjeongkong # 3, and # 4 grown at higher latitudinal Suwon were higher Milyang while that in Milyang # 113 grown at lower latitudinal Milyang was higher compared to higher latitudinal Suwon. Genistein contents in most black soybean cultivars except Milyang # 113, and total isoflavone contents in Gemjeongkong # 4 and Cheongjakong grown at lower latitudinal Milyang were higher compared to higher latitudinal Suwon. The variations of anthocyanin except Pt3G and isoflavone contents seemed to be affected by environmental conditions like different latitude than the genotype because they showed the significant interaction between cultivars and locations.