연구논문 : 자연과학 ; 남부지역에서 비닐피복에 따른 콩 생육특성 및 수량성 차이

백인열 ( In Youl Baek ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.1

Nowadays P. E. film mulching for weed control and moisture preservation is popular in soybean cultivation of southern region of Korea. Some merits of P. E. film mulching are moisture preservation, soil aggregation, weed control and growth promotion due to increase soil temperature. The rearrangement of seeding date of soybean is necessary to prevent overgrowth of soybean and promote healthy growth. As global warming increases average world temperature, the demanding of re-establishing soybean cultivation system, such as seeding date, has been increased. Therefore, we discussed seeding date accompanying maturity types as soybean was cultivated on condition of P. E. film mulching. Four varieties, Saeol, Seonyu, Daewon and Pungsannamul were seeded on May 10, May 25, June 10 and June 25 in P. E. film mulching and non-mulching field. It is required more mean days of flowering in nonmulching and more days of maturity in P. E. film mulching. In R5 stage completed organ structure dry matter weight was heavier and LAI was ranged 5 to 7 which was optimum LAI. Number of pods and number of seeds per plant in P. E. film mulching were higher on June 25 seeding and 100-seed weight was heavier through all seeding date of P. E. film mulching. Seed yield in P. E. film mulching cultivation was higher on May 25 and June 10 seeding. Occurrence of damaged seeds showed no difference between two cultivation types and among seeding dates. Major diseases and insect pests were bacterial pustule, black root rot, wild fire and stink bugs. And that, occurrence of root rot and stink bugs influenced to yield decrease. Protein content of soybean seed in P. E. film mulching was lower in all seeding dates. Fatty acid compositions showed no difference between two culivation types and among seeding dates. But C18:1 composition was higher on May 10 and May 25 seeding in P. E. film mulching cutivation and C18:3 composition was similar between two cultivation types. Isoflavone content was higher in P. E. film mulching cultivation and higher as seeding date was delayed. Summerizing above results optimum seeding date for P. E. film mulching cultivation ranged from May 25 to June 10 in southern region of Korea.

Variation of yield and seed component in delayed planting cultivated in southern region of Korea tested with currently developed soybean cultivars

Hyeon-Jin Park,Ki-Won Oh,Won-Young Han,Hyun-Tae Kim,Sang-Ouk Shin,Jin-Woo Bae,Byung-Won Lee,Jong-Min Ko,In Youl Baek 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

In southern region of Korea, double cropping system including soybean as a second crop is widely adopted because prolonged high temperature in October enables soybean maturation even in late autumn. Global warming during decades derives transition of soybean planting dates as late as late-July. Delayed planting beyond the critical time moves reproductive stage to less favorable weather conditions like low temperature and short photoperiod, which can affect soybean yield and seed composition. Therefore selection of appropriate cultivar for changed climate and cultural practice is required. Effects of different eco-type and planting date on soybean yield was investigated for three sowing dates of 20 June, 5 July and 20 July with ten cultivars. Strong interaction between cultivar and sowing date was detected in number of branch, node and pod. No significant interaction was observed in 100-grain weight and yield, and this might be resulted from similar yield reduction pattern. Daepung, Taekwang and Uram recorded the highest yield when planted late, proving that these three cultivars are the most adaptable to delayed planting. Delayed planting results in higher sugar content, especially stachyose and raffinose which have detrimental effect on human health by causing flatulence and diarrhea. Protein content was decreased and oil content was increased in July 20 planting. Decrease in protein content was the largest in Hwangeumol and maintained high in Saedanbaek. This information will provide a guideline when select appropriate soybean cultivar in delayed planting to maintain yield and seed quality.

오존수가 콩의 발아와 콩나물 생장에 미치는 영향

백인열,박규환 한국콩연구회 2000 韓國콩硏究會誌 Vol.17 No.2

In order to develop the clean culture method for soybean sprout culture, ozone water and ozone were treated in the cu1W re of soybean sprout. Soybean seeds were soaked in different concentration of ozone water for 4 hours and then they were sprinkled with ozone water every 4 hour in culture beds kept at 25℃. The pattern of water absorption of soybean was different among varieties. Absorption of water was taken rapidly 6 hours after soaking, and then slowly. It took 2 hours after soaking to reach the 50% absorption of water. After 12 hours soaking, soybean seeds were saturated with water. Germination percentage of soybean seeds soaked was not differed over 4 hours soaking. At least, more than 4 hours in soaking is necessary to the culture of soybean sprout. If water sprinkled on soybean. 3 hours in soaking is sufficient for germination. Optimum temperature for germination after soaking was 20∼25℃. Initial germination rate was higher than control when soaking in ozone water. The proper concentration of ozone water was 0.3ppm. The growth of soybean sprout grown with 0.3ppm ozone water was better than control in whole length, hypocotyl length, and sprout yield. As ozone treated in air, the growth of soybean sprout was shorter than control in length and then hypocotyl was bent.

A New Black Soybean Cultivar, “Geomjeongsaeol” with Large Seed,Early Maturity and Lodging Tolerance

In-Youl Baek,Keum-Yong Park,Jung-Kyung Moon,Yong-Ho Kim,Sun-Lim Kim,Ja-Hwan Ku,Yeong-Ho Lee,Yong-Hwan Ryu,In-Youl Baek,Won-Young Han,Hyun-Tae Kim,Jun-Hyeon Cho,Jong-Min Ko,Doo-Chull Shin,Duck-Yong Suh 한국육종학회 2005 한국육종학회지 Vol.37 No.5

A new black seed coated soybean cultivar with yellow cotyledon, Geomjeongsaeol was developed at the YeongnamAgricultural Research Institute (YARI) in 2004. Geomjeongsaeol was selected from a cross Keunol/ Geomjeongol. The prelimi-nary, advanced, and regional yield trials for evaluation and selection of this line (Milyang 123) were carried out from 1999 tospherical seed shape, oval leaflet shape, and large seed size (31.1 grams per 100 seeds), and it was 4 days later in maturity than thecheck variety Geomjeongol. In the seed quality such a crude protein, oligo-saccharide, and anthocyanin contents, Geomjeongsaeolwas higher than check variety, and it has good adaptability for cooking with rice. It also has been identified to have resistance tosaeol was 2.34 ton per hectare in the regional yield trials (RYT) carried out for two years from 2003 to 2004 which was 26 percenthigher than the check variety Geomjeongol.

콩 발아시 오존수 처리에 따른 효소활성 변화

백인열,박규환 한국콩연구회 2000 韓國콩硏究會誌 Vol.17 No.2

Changes in amylase, cellulase, and proteinase in the cotyledons and embryo axes of soybean(Glycine max cv. Eunhakong) seeds, were determined over a 6-day germination period. Day-0 samples of seeds were soaked in the strong oxidant of ozone treated water(0.3ppm) for 4 hour and cultured in ozone water(six times a day) of 25℃ in a germinator. In the non-treatment of ozone water, amylase and cellulase activity of embryo axes decreased more rapidly throughout the germination period while those of cotyledons increased steadily during the first 4 days and declined thereafter. And total amylase and cellulase activity of the sprouting seeds decreased steadily during the first 4 days and decreased rapidly after the fourth day. Proteinase activity of embryo ayes and cotyledons increased slightly during the germination period, but those of embryo axes decreased steadily after the fourth day. In the treatment of ozone waters, amylase of cotyledons was high activity during the first 2 days and was low activity thereafter in comparison to non-treatment while that of embryo axes was low activity only in the first days and was similar activity thereafter. Cellulase of embryo axes and cotyledons was low activity during the 2∼4 days while that of embryo axes was high activity in the first days and the 6 days in comparison to non-treatment. Proteinase of cotyledons was high activity throughout the germination period while that of embryo axes was low activity during the first 2 days and was high activity thereafter in comparison to non-treatment.

Evaluation of late-sowing-adaptable soybean cultivar in paddy field

Han Won Young,Park Hyeon Jin,Oh Ki Won,Kim Hyun Tae,Shin Sang Ouk,Lee Byong Won,Ko Jong Min,Baek In Youl 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

Soybean self-sufficiency in Korea was 22.5% in year 2011, and as free trade agreement between Korea and US comes into effect, the amount of soybean importation increases. In 2012, paddy field soybean cultivation was over 0.1 million ha and it is expected to increase continuously due to rise in market price of soybean. Moreover, double cropping system including paddy field soybean is widely adopted nationwide, but studies on appropriate cultivar for this environment are insufficient. In this research, the effect of planting date and different cultivars on soybean growth and yield was investigated for three planting dates (June 20, July 5, and July 20) with 15 cultivars. According to ANOVA test, soybean yield was significantly different depending on sowing date and cultivars and interaction between sowing date and cultivars was also detected, meaning each of cultivars resulted different yield depending on sowing date. When planted on July 20, stem length, nod number, branch number and grain weight was decreased because of short growing period, resulting in yield decrease. Percentage of yield obtained from July 20 in contrast to that of June 20 showed that Pungwon, Nampung and Daepung recorded the least yield decrease and the highest yield when planted late. Evaluation of late-sowing-adaptable soybean cultivar in paddy field is now being carried on following last year.

Variation of Anthocyanin, and Isoflavone Contents in Korean Black Soybean Cultivars Grown at Different Latitudinal Locations

Seung-Beom Hong,Su-Jin Lee,Young-Hak Kim,Young-Sun Hwang,Kwang-Hee Yoon,Sung-In Lee,Mi-Young Nam,Lee-Seul Song,In-Youl Baek,Myoung-Gun Choung 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Anthocyanin pigments in black soybean seed coat have been known as including biological activities, which are antitumor, antioxdant and antivirus. Also in soybean, isoflavone have been reported as health-promoting benefits, which are antiestrogen, antioxidant, antitumoral activities and preventing cardiovascular diseases and osteoporosis. The consumption of soybean increased in Korea because of the importance of its functional characteristics of the anthocyanin and isoflavone. Therefore, this experiment was conducted to investigate the variation of anthocyanin, and isoflavone contents in Korean domestic black soybean cultivars grown at different latitudinal locations, Suwon (37°16'N) and Milyang (35°30'N). D3G, C3G, and total anthocyanins in Geomjeongkong # 3, C3G, and total anthocyanins in Ilpumgeomjeongkong grown at higher latitudinal Suwon were higher compared to lower latitudinal Milyang. Daidzein, glycitein, genistein, and total isoflavone contents in Gemjeongkong # 4 were highest among the seven cultivars. Daidzein contents in Gemjeongkong # 3, Milyang # 112, and Milyang # 113 grown at higher latitudinal Suwon were higher compared to lower latitudinal Milyang. Glycitein contents in Gemjeongkong # 3, and # 4 grown at higher latitudinal Suwon were higher Milyang while that in Milyang # 113 grown at lower latitudinal Milyang was higher compared to higher latitudinal Suwon. Genistein contents in most black soybean cultivars except Milyang # 113, and total isoflavone contents in Gemjeongkong # 4 and Cheongjakong grown at lower latitudinal Milyang were higher compared to higher latitudinal Suwon. The variations of anthocyanin except Pt3G and isoflavone contents seemed to be affected by environmental conditions like different latitude than the genotype because they showed the significant interaction between cultivars and locations.

Distribution of Fatty Acids in Newly Developed Tissues of Soybean Seedlings

Krishna Hari Dhakal,Yeon Shin Jeong,Tae Joung Ha,In Youl Baek,Young Keun Yeo,Young Hyun Hwang 한국육종학회 2011 한국육종학회지 Vol.43 No.1

The objective of this study was to determine the fatty acid composition of newly developed tissues of germinated soybean seeds. Five soybean accessions with varied fatty acid composition were allowed to germinate in sand under greenhouse conditions. Seedlings were picked up after 4, 6, 8, 10 and 12 days of germination and freeze dried. The fatty acid composition of the newly developed tissues was analyzed by gas chromatography. Significant variation in fatty acid composition was observed between accessions, days of germination, and variety x day of germination in whole and the cotyledons. In the case of newly developed five tissues, significant variation in fatty acid composition were observed between days of germination except oleic acid for root, hypocotyl and epicotyl stem and except stearic acid for hypocotyl and unifoliate leaves while all the parameters were significantly different for accession. Significant interactions of accession and days of germination were observed for palmitic, linoleic and linolenic acid in all tissues; only for oleic acid in hypocotyl, epicotyl and unifoliate leaves; and only for stearic acid in root, hypocotyl, epicotyl and unifoliate leaves. During germination, the fatty acid composition of newly developed tissues changed dramatically but whole seedlings and cotyledons changed slightly. These tissues contained five major fatty acids as found in original seeds, but compositions were totally different from that of the seed: higher in palmitic, stearic and linolenic acid and lower in oleic and linoleic acid. New tissues conserved their fatty acid compositions regardless of genotypic variation in the original seeds.

Fatty Acid Composition in each Structural Part of Soybean Seed and Sprout

Dhakal, Krishna Hari,Jeong, Yeon-Shin,Lee, Jeong-Dong,Baek, In-Youl,Ha, Tae-Joung,Hwang, Young-Hyun 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.2

Fatty acid composition and $C_{18:2}:C_{18:3}$ of soybean seed and sprout is important for soy-industries to manufacture quality products. Six soybean varieties were analyzed by gas chromatography to determine fatty acid composition and linoleic to linolenic acid ratio ($C_{18:2}:C_{18:3}$) in seed, sprout, and their structural parts. In the case of whole seed and its structural parts, significant variation in fatty acid composition and $C_{18:2}:C_{18:3}$ were observed between varieties except palmitic acid $(C_{16:0})$, while all the parameters were significantly different for seed parts. Significant interactions of variety with seed parts were observed for oleic acid $(C_{18:1})$, $C_{18:2}$ and $C_{18:2}:C_{18:3}$. The highest saturated fatty acid, $C_{18:1}$ and polyunsaturated fatty acid were recorded in seed coat, cotyledon, and seed axis, respectively. The lowest $C_{18:2}:C_{18:3}$ was found in seed axis. In the case of sprout study, variety had significant effect on all the parameters observed for whole sprout, cotyledon, root, and except $C_{18:3}$ for hypocotyl. Culture days had significant effect on $C_{16:0}$, $C_{18:2}$ and $C_{18:3}$ in whole sprouts, while only on $C_{18:3}$ and $C_{18:2}:C_{18:3}$ in cotyledon. For hypocotyl, culture days had significant effect on $C_{18:1}$, $C_{18:2}$ and $C_{18:2}:C_{18:3}$, however, $C_{16:0}$, $C_{18:1}$, $C_{18:3}$, and $C_{18:2}:C_{18:3}$ were significantly different in root. In sprout, days and variety interacted significantly for $C_{16:0}$, $C_{18:3}$ and $C_{18:2}:C_{18:3}$, and $C_{18:1}$, $C_{18:2}$, $C_{18:3}$, and $C_{18:2}:C_{18:3}$, respectively for whole, cotyledon, and root. The significantly lowest $C_{18:2}:C_{18:3}$ (1.1) was observed in hypocotyl and root of Hwangkeumkong in 5 days. Knowledge of fatty acid composition and $C_{18:2}:C_{18:3}$ of seed, sprout, and their parts could be applicable for oil and other soy-food industries to make quality products.

Genetic Analysis of Pod Dehiscence in Soybean

Sung Taeg Kang,Hyeun Kyeung Kim,In Youl Baek,Moung Gun Chung,Won Young Han,Doo Chull Shin,Suk-Ha Lee 韓國作物學會 2005 Korean journal of crop science Vol.50 No.4

Pod dehiscence (PD), defined as the opening of pods along both the dorsal and ventral sutures, causes the seed to shatter in the field before harvesting and results in loss of seed yields. However, breeding for resistance to PD is difficult due to the complicated genetic behavior and environmental interaction. The objective of the present research was to analyze the genetic behavior of PD for improving the breeding efficiency of resistance to PD in soybean. PD after oven-drying the sampled pod at 40~circC for 24 hours was the most reliable to predict the degree of PD tested in the field. Keunolkong, a dehiscent parent, was crossed with non-dehiscent parents, Sinpaldalkong and Iksan 10. Using their F1~;and~;F2 seeds, PD was measured after oven drying the pod at 40~circC for 24 hours. The gene conferring PD behaved in different manners depending on the genetic populations. In the Keunolkong~times Sinpaldalkong population, PD seemed to be governed by single major recessive gene and minor genes, while several genes were probably involved in the resistance to pod dehiscence in the Keunolkong~times Iksan 10 population. Heritability for PD estimated in F2 population showed over 90~% in the two populations. High heritability of PD indicated that selection for resistance to PD should be effective in a breeding program. In addition, genetic mapping of quantitative locus (QTL) for PD in both populations may reveal that genes conferring PD are population-specific