Sauchinone Suppresses Pro-inflammatory Mediators by Inducing Heme Oxygenase-1 in RAW264.7 Macrophages

Li, Bin,Lee, Dong-Sung,Choi, Hyun-Gyu,Kim, Kyoung-Su,Kang, Dae-Gil,Lee, Ho-Sub,Jeong, Gil-Saeng,Kim, Youn-Chul Pharmaceutical Society of Japan 2011 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.34 No.10

<P>Sauchinone, a biologically active lignan isolated from the roots of <I>Saururus chinensis</I> (L<SMALL>OUR</SMALL>.) B<SMALL>AILL</SMALL>. (Saururaceae), is reported to exert a variety of biological activities, such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to a reduction in COX-2-derived prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) and iNOS-derived nitric oxide (NO) production in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages. Present study also demonstrates the effects of sauchinone in inducing heme oxygenase-1 (HO-1) expression and an increase in heme oxygenase (HO) activity in RAW264.7 macrophages. The effects of sauchinone on LPS-induced PGE<SUB>2</SUB>, NO, tumor necrosis factor-α (TNF-α) and interlukine-1β (IL-1β) production were partially reversed by the HO-1 inhibitor Tin protoporphyrin was also seen in this study. In addition, we found that treatment with extracellular signal-regulated kinase (ERK) inhibitor (PD98059) reduced sauchinone-induced HO-1 expression. Sauchinone also increased ERK phosphorylation. These results suggest that sauchinone inhibits pro-inflammatory mediators through expression of anti-inflammatory HO-1 <I>via</I> ERK pathway.</P>

광미를 사용한 폴리머 시멘트 콘크리트의 강도 특성 및 내약품성

이병철,이주경,이윤수,이필호,이구연,전철수 강원대학교 부설 석재복합신소재 제품연구센터 1999 석재연 논문집 Vol.4 No.-

본 연구는 세골재의 일부를 광미로 치환하고 혼화제로 SBR 라택스를 사용하여 폴리머 시멘트 콘크리트 공시체를 제작한 후 강도특성 및 내약품성을 실험적으로 구명한 것이다. 그 결과 광미를 사용하지 않고 P/C비가 10%일 때 압축강도가 323㎏/㎠로 최고 값을 나타냈으며, 휨강도는 P/C비가 증가할수록 증가하여 P/C비가 10%일 때 89㎏/㎠를 나타냈다. 그리고 광미를 사용한 경우는 P/C비가 10%일때 압축강도가 357㎏/㎠, 휨강도가 92㎏/㎠로서 광미를 사용하지 않은 경우보다 높게 나타났다. 한편 10% HCI, 20%, NaOH 10% NaCl 수용액에 공시체를 3, 7, 14, 21, 28일간 침적 시킨 후 중량변화를 측정하여 내약품성을 실험한다. 광미를 사용한 경우가 우수한 내약품성을 나타냈다. 즉광미를 사용하지 않은 경우 P/C=0%일 때 28일 후 중량 변화가 11%였으나 P/C=20%일 때는 2%였고, 광미를 사용한 경우는 P/C=0%일 때 질량 변화가 6%였으나 P/C=20%일 때는 1.5%였으며 20% ,NaOH 10% NaCl 수용액에서는 질량이 증가하는 경향을 보였다. Polymer cement concrete is made by the modifying ordinary cement concrete with polymer additive. Until now polymer cement concrete is not used for the structural member, but it is growing to be considered as developing uses such as a waterproof of roof slab, and a road pavement. The polymer cement concrete, being used for those uses, is superior to the cement concrete against the inorganic, organic acid, salt of acetic acid and organic solvents generally. In this paper, the polymer cement concrete was made by the ratio of 1:1 of sands and metal-mine waste rocks in fine aggregate in order to solve the environmental pollution which causes the social problem by metal-mine waste rocks. It was measured for the compressive strength, flexural strength, and chemicals resistance was tested by dealing with 10% HCI, 20% NaOH and 10% NaCI aqueous solution.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

크롬(Ⅵ)의 체내 흡수와 소실속도에 관한 연구

김현영,이성배,임철홍,이권섭,정용현,이종성,한정희,전윤석,황호순,이용묵 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1

The CrO3 mostly used in plating. metal surface disposal, leather, cosmetic manufacturing, as an experiment material by repeatedly inhaling and exposure the male S.D. rats at a 0.00, 0.2., 0.50, 1.25 mg/㎡ concentration(particle size: 0.5-0.5 aerosol)6hours a day, 5day a week in 13weeks comparing with 2weeks, 8weeks of recovery group about the noxiousness of the experiment animal and the reduce scale of the CrO3 in the internal organ especially in blook and respiratory organ with the period of convalescent and clearance. The experiment results which we received are as follows. 1. In blood the RBC, HGB and HCT experiment, rats with 0.20, 0.50 mg/㎡ concentration showed that there were some decreases but not dependent. The kidneys absolute weight compared with control group was reduced intentionally(p〈0.05) and the lungs absolute weight compared with control group showed intentional increase(p〉0.05). 2. After the exposure of the experiment material, the whole blook, l\blood plasma and red blood cell in blood by (x): the period of convalescent, per (y); the decreasing of Cr concentration, was y=66.51 e -0.057x, y=67.2 e-0.101x, y=70.01 e-0.030 in 0.50 mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life (day)was estimated 12.0, 6086, 23.0 each. 3, After the exposure of the experiment material, the experiment animals lung, liver and kidneys by(x); the period of convalescent, per (y); the decreasing of Cr conentration, was y=1808 e-0.00493x, y=12.02e-0.0297x, y=67.61 e-0.0292x in 0.50mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life(day)was estimated 140.6, 23.3, 23.7, each, and including lung, liver with all of the experiment internal organs, the Cr clearance decreased as the exposure concentration increased.

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

Fluoxetine and Sertraline Attenuate Postischemic Brain Injury in Mice

Tae Kyeong Shin,Mi Sun Kang,Ho Youn Lee,Moo Sang Seo,Si Geun Kim,Chi Dae Kim,Won Suk Lee 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

This study aimed to investigate whether selective serotonin reuptake inhibitors (SSRIs) attenuate brain injury and facilitate recovery following photothrombotic cortical ischemia in mice. Male ICR mice were anesthetized and systemically administered Rose Bengal. Permanent focal ischemia was induced in the medial frontal and somatosensory cortices by irradiating the skull with cold light laser. The animals were treated with fluoxetine or sertraline once a day for 14 d starting 1 h after ischemic insult. Treatment with fluoxetine and sertraline significantly reduced the infarct size. The Evans blue extravasation indices of the fluoxetine- and sertraline-treated groups were significantly lower than that of the vehicle group. Treatment with fluoxetine and sertraline shifted the lower limit of the mean arterial blood pressure for cerebral blood flow autoregulation toward normal, and significantly increased the expression of heme oxygenase-1 (HO-1) and hypoxia-inducible factor-1Ձ (HIF-1Ձ) proteins in the ischemic region. These results suggest that SSRIs, such as fluoxetine and sertraline, facilitate recovery following photothrombotic cortical ischemia via enhancement of HO-1 and HIF-1Ձ proteins expression, thereby providing a benefit in therapy of cerebral ischemia.

Fluoxetine and Sertraline Attenuate Postischemic Brain Injury in Mice

Shin, Tae-Kyeong,Kang, Mi-Sun,Lee, Ho-Youn,Seo, Moo-Sang,Kim, Si-Geun,Kim, Chi-Dae,Lee, Won-Suk The Korean Society of Pharmacology 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

This study aimed to investigate whether selective serotonin reuptake inhibitors (SSRIs) attenuate brain injury and facilitate recovery following photothrombotic cortical ischemia in mice. Male ICR mice were anesthetized and systemically administered Rose Bengal. Permanent focal ischemia was induced in the medial frontal and somatosensory cortices by irradiating the skull with cold light laser. The animals were treated with fluoxetine or sertraline once a day for 14 d starting 1 h after ischemic insult. Treatment with fluoxetine and sertraline significantly reduced the infarct size. The Evans blue extravasation indices of the fluoxetine- and sertraline-treated groups were significantly lower than that of the vehicle group. Treatment with fluoxetine and sertraline shifted the lower limit of the mean arterial blood pressure for cerebral blood flow autoregulation toward normal, and significantly increased the expression of heme oxygenase-1 (HO-1) and hypoxia-inducible factor-1 ${\alpha}$ (HIF-1 ${\alpha}$) proteins in the ischemic region. These results suggest that SSRIs, such as fluoxetine and sertraline, facilitate recovery following photothrombotic cortical ischemia via enhancement of HO-1 and HIF-1 ${\alpha}$ proteins expression, thereby providing a benefit in therapy of cerebral ischemia.

Effects of Herbal Extracts Used in Oriental Medicines on Heme Oxygenase-1 Expression

Jeong, Gil-Saeng,Oh, Seung-Hwan,Kang, Dae-Gill,Lee, Ho-Sub,Kim, Youn-Chul The Physiological Society of Korean Medicine and T 2006 동의생리병리학회지 Vol.20 No.5

Effects of twenty-three aqueous herbal extracts used in oriental medicines on heme oxygenase (HO)-1 expression were estimated in a mouse hippocampal cell line, HT22. HO-1 is one of the cytoprotective enzymes activated various stimuli, and Western blot analysis was used for evaluated HO-1 expression. Six aqueous extracts such as Rhei Rhizoma, Paeoniae Radix, Uncariae Ramulus et Uncus, Theae Folium, Prunellae Spica, and Coptidis Rhizoma significantly increased HO-1 expression in HT22 cells at the concentration of 300 ${\mu}$g/ml. In Addition, four aqueous extracts including Eucommiae Cortex, Moutan Cortex Radicis, Ginseng Radix Rubra, and Scutellariae Radix moderately increased HO-1 expression. These results would be usefulfor the isolation and identification of their neuroprotective principles.

Silicoater technique을 이용한 veneered stainless steel crown 수복의 임상적 평가

이상호,이창섭,김왕근,정연화 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

광법위한 치질파괴가 있는 상악유전치를 갖는 소아에서 silicoater technique에 의한 veneered stainless steel crown으로 수복 후 3∼12개월 동안 주기적 검사와 수복물의 평가 결과, 다음과 같은 결론을 얻었다. 1. 행동조절이 어려운 소아에서 chair time을 감소시킴으로써 행동조절에 도움을 주었다. 2. 유지력,내구성,심미성이 우수하였으므로 유전치부의 광법위한 치질손상시 유용한 수복방법이다. Restoration of severly carious, malformed or traumatically fractured primary incisors is one of the most difficult challenges in restorative dental care for preschloers. In restoring primary anterior teeth, four types of complete coverage crowns have been attempted over the years. Stainless steel crowns, open-faced stainless steel crowns, acid-etched crowns(strip crowns), and preformed polycarbonate crowns have been the choeces. While these restorations have beem accepatable for many patients, they all have very distinct limitations. The ideal full coronal restoration for a primary incisor requires durability, retention, and esthetics. This paper reported veneered stainless steel crown restoration of primary anterior teeth by silicoacter technique. Veneered stainless steel crown restoration by silicoater technique is considered to be a durable, retentive, and esthetic restoration.

Ca^(2+)-감수성 증가기전과 평활근 수축

이연리,김보경,최원호,김태경,이창권,배영민,조성일 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Smooth muscle contraction is regulated by intracellular Ca^(2+) ([Ca^(2+)]_(i)) and the phosphorylation of myosin light chain (MLC). However, various kinds of vasoconstrictors induce a further contraction at a given [Ca^(2+)]_(i), and elicit a sustained contraction under Ca^(2+) -depleted conditions, referred to as "Ca^(2+) -sensitization", in intact and membrane-pen-neabilized smooth muscle. Previously, several molecules, including protein kinase C (PKC), RhoA, and mitogen-activated protein kinases (MAPK), have been suggested as candidate regulators of Ca^(2+) -sensitization. In the present review, we describe the role of PKC, RhoA, and MAPK in the regulation of Ca^(2+) -sensitization, and suggest a new model in research for the regulation of smooth muscle contraction.