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Heechul Kim,Seungjoon Kim,Jeeyoung Lee,Jeongtae Kim,Jin Won Hyun,Jae Woo Park,Sung-Ho Kim,Changjong Moon,Taekyun Shin 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.2
Radiation therapy is used for cancer treatment, but there are some side effects such as oxidative damage. Sodium salicylate, an anti-inflammatory drug, plays a role in the reduction of oxidative damage. We investigated the antioxidant role of sodium salicylate against gamma-radiation-induced oxidative damage in liver tissue after whole-body irradiation with a single dose of 5 Gy. Twenty adult rats were divided into four equal groups. On the day of the experiment, groups Ⅰ and Ⅱ were treated orally with 200 ㎎/㎏ sodium salicylate, while groups Ⅲ and Ⅳ received distilled water. One hour later, groups Ⅱ and Ⅳ were exposed to 5 Gy whole-body ionizing radiation in a single fraction. Thiobarbituric acid-reactive substances (TSARS) in the liver ware measured in all groups, 4 hours after their exposure to radiation. Whole-body radiation significantly increased TSARS level in the liver, while sodium salicylate significantly reduced hepatic TSARS level in irradiated rats. Furthermore, radiation injury induced Kupffer cell activation, while sodium salicylate significantly inhibited Kupffer cell activation in irradiated rats. The data obtained in this study suggest that sodium salicylate administration prior to irradiation may prevent liver damage by radiation.
A functional properties of apple blossoms by variety as a cosmetic material
Heechul Kim,Eunbee Lee,Songhyeon Kim,Eungyeong Lee,Jeongmin Yun,Seonjeong Kim,Changil Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study investigated whether apple blossoms are functional as a cosmetic material. In this study, Crap apple, Hong-ro, Summer king, and Fuji varieties were used as samples. For each sample, general component analysis and functional tests were conducted, and total phenol contents, DPPH, ABTS, and PF tests were performed to find out antioxidant activity as functional tests. As a result of analyzing the general components of apple blossom, crude carbohydrates were 8.321∼11.425%, crude protein was 3.919∼4.724%, crude fat was 2.902∼3.430%, crude ash was 1.141∼1.218%, and moisture was 79.959∼83.112%. DPPH is 3.25% (25 μg/mL), 6.48% (50 μg/mL), 13.54% (100 μg/mL), ABTS is 10.77% (25 μg/mL), 23.16% (50 μg/mL), 45.60% (100 μg/mL), Crap apple showed the highest antioxidant activity. Also, the total phenol content was 173 mgGAE/g, which was the highest in the Crap apple sample. However, there was no significant difference in the PF values indicating the fat-soluble antioxidant activity among apple blossom varieties.
Heechul Park,Sung-Bae Park,Junseong Kim,Hyeonjeong Jeon,Sein Choi,Seungyeon Lee,Eunchong Oh,Soenghwi Hwang,Hyunjung Kim,Jungho Kim,Sunghyun Kim 대한의생명과학회 2020 Biomedical Science Letters Vol.26 No.3
Staphylococcus aureus (S. aureus) is known as a bacterium that can cause skin infections, respiratory system infections, and sinusitis; however, it can exist as a normal flora rather than a pathogen. Recently, methicillin-resistant S. aureus (MRSA) infections have emerged in the community as a new variant of community-associated (CA)-MRSA. In the present study, S. aureus and MRSA were isolated and cultured by collecting samples from facilities and environments where students and educational personnel have multiple contacts on university campuses; specifically, the nostrils and hands of college students were tested from July to September of 2019. The molecular properties of the isolated MRSA were analyzed, and the one MRSA strain was isolated from the university campuses. One MRSA that was isolated and cultured on campus was the mec complex group A and staphylococcal cassette chromosome (SCC) mec type II, which is a characteristic of healthcare-associated (HA)-MRSA, and SCCmec type V, which is a characteristic of CA-MRSA. This result was similar to other studies wherein the SCCmec type II was detected in SCCmec typing analysis in CA-MRSA. To confirm whether there is a new variant of CA-MRSA in the Republic of Korea, additional follow-up studies on the analysis of virulence factors of MRSA are needed by additionally separating CA-MRSA from the body parts of university students and educational personnel.
Immunohistochemical Study of Osteopontin in Rat Brain with C6 Gliomas
Heechul Kim,Jeongtae Kim,Do-Hyun Nam,Ki-Bum Sim,Yongduk Lee,Seungjoon Kim,Taekyun Shin 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.2
Immunohistochemical analysis of osteopontin (OPN) was studied in the brain of rats with implanted C6 glioma to see the involvement of OPN in the pathogenesis of C6 glioma in in vivo. Three lesions including tumor cell infiltration area, compact tumor growth area and contralateral area were compared at day 11 post-implantation. OPN was immunostained in the extracellular matrix, as well as in some ED1 (anti-rat macrophage)-positive macrophages/activated microglia, but few in astrocytes, in the tumor cell infiltration area. In the compact tumor growth area, some glial fibrillary acidic protein-positive glioma cells were also stained for OPN, while OPN immunoreactivity was very weak in ED1 positive macrophages. In contralateral lesion of C6 glioma implantation and normal control brains, OPN was only found in few neurons and vascular endothelial cells, but not in glial cells. These results suggest that OPN may be involved in the tumor-associated activation of macrophages, particularly in the lesion of tumor infiltration, and be involved in the proliferation and expansion of astrocytes in the course of rat C6 glioma models.
Regioselective Addition Reactions of the Organoindium Reagents onto α, β-Unsaturated Ketones
이필호,Hyun Kim,이구연,Dong Seomoon,Sundae Kim,Heechul Kim,Hyunseok Kim,Miae Lee,Eunkyong Shim,Seokju Lee,Misook Kim,Mijeong Han,Kwanghyun Noh,Madabhushi Sridhar 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.11
Regioselectivity on the reactions of α,β-enones with organoindium such as in situ generated allylindium and allenylindium was systematically studied in the presence of TMSCl as an additive. Treatment of 2-cyclohexen- 1-one, carvone, 2-cyclohepten-1-one, and chalcone with allylindium reagent produced 1,4-addition products in good yields, while 2-cyclopenten-1-one, 2-methyl-2-cyclopenten-1-one, 4,4-dimethylcyclohexen-1-one, 3- nonen-2-one, 4-hexen-3-one, and 4-phenyl-3-buten-2-one afforded 1,2-addition products. Indium reagent derived from indium and propargyl bromide in Grignard type gave addition products in good yields, under which the successive addition of α,β-enone and TMSCl were necessary. Although organoindium reagent derived from propargyl bromide produced propargylated compound in Grignard type except 2-cyclohepten-1- one, indium reagent obtained from 1-bromo-2-butyne having γ-methyl group gave allenylated product in Barbier type.
환자의 비강으로부터 분리된 메티실린 내성 황색 포도알균의 분자 아형 분석
Sang-Ha Kim,Sung-Bae Park,Heechul Park,Jun Seong Kim,Jungho Kim,Jiyoung Lee,임재원,Young Kwon Kim,Sunghyun Kim 대한임상검사과학회 2020 대한임상검사과학회지(KJCLS) Vol.52 No.2
Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that causes infections in different parts of the body and causes skin and soft tissue infections (SSTI). The present study examined the antimicrobial resistance patterns and molecular epidemiological characteristics of MRSA isolated from nasal swabs in clinical patients. SCCmec type of MRSA isolates from clinical patients were analyzed: 24 cases were SCCmec type-II; two cases were type-II/IVa; one case was type-II/V; one case was type-IVa; 11 cases were not-typeable. The mec complex type of MRSA isolates from clinical patients were analyzed: 29 cases were mec complex type A, and 10 cases were not-typeable, but type B was not found in the present study. In conclusion, SCCmec type-II and mec complex type A were the most dominant MRSA subtypes among the MRSA isolates from a nasal swab of patients, and the results were similar to other studies on hospital-acquired MRSA (HA-MRSA). These results can not only provide basic data for hospital infection management but also be a good guideline for MRSA infections in the Republic of Korea. 본 연구에서는 환자의 비강으로부터 분리 배양된 총 39건의 MRSA 분리 배양 균주를 이용해 mecA 유전자 검출, SCCmec typing과 mec complex typing을 분석해 보고자 하였다. 임상 환자의 비강으로부터 분리 배양된 MRSA 총 39주 중 SCCmec type-II가 24건, type-II/IVa가 2건, type-II/V가 1건, type-IVa가 1건, not-typeable이 11건으로 분석되었으며, mec complex type A가 29건, not-typeable이 10건이었으며, type B는 없는 것으로 분석 되었다. 결론적으로, 환자의 비강으로부터 분리된 MRSA 분리 배양 균주 중 SCCmec type-II와 mec complex type A 아형이 가장 많이 분포하고 있었으며, 이 결과는 의료기관 획득 MRSA에 관한 다른 연구결과와 유사한 결과를 나타냈다. 이후 환자의 비강으로부터 분리 배양된 not-typeable 아형의 MRSA 균주를 대상으로 국내에서 발견되는 새로운 MRSA 아형 규명에 관한 추가 연구가 필요할 뿐만 아니라, MRSA 분리 배양 균주의 아형을 분석함으로써 그 분자적 특성을 분석한 결과를 바탕으로 병원 감염관리를 위한 기초자료를 제공할 수 것으로 사료된다.
( Heechul Nam ),( Sung Won Lee ),( Jung Hyun Kwon ),( Hae Lim Lee ),( Sun Hong Yoo ),( Hee Yeon Kim ),( Do Seon Song ),( Pil Soo Sung ),( U Im Chang ),( Chang Wook Kim ),( Soon Woo Nam ),( Si Hyun Bae 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Antiviral therapy improves hepatic fibrosis and reduces hepatocellular carcinoma (HCC) incidence. This study aimed to evaluate whether on-therapy changes in scores for fibrosis index based on four factors and aspartate aminotransferase-to-platelet ratio index are associated with HCC development and establish an HCC risk score model incorporating non-invasive fibrosis marker (NFM) response. Methods: This multi-center study recruited 5147 chronic hepatitis B patients (4028 for derivation cohort, 1119 for validation cohort) who were given Entecavir/Tenofovir for >12 months between 2007 and 2018. A risk prediction model for HCC was developed using predictors based on multivariable Cox models and bootstrapping was performed for validation. Results: The 10-year cumulative HCC incidence rates were 12.6% and 13.7% in the derivation and validation cohorts, respectively. The risk of HCC significantly differed with early NFM response, with a marked reduction in HCC risk in patients achieving a significant decrease in NFM by 12 months (P<0.001). Sex, age, cirrhosis, and NFM response were independently predictive of HCC, and the FSAC model was developed based on these variables. For the 10-year prediction of HCC, FSAC showed higher c-index values than PAGE-B, CU-HCC, and REACH-B (0.84 vs. 0.76, 0.77, and 0.67, respectively; all P<0.001). The predictive performance of FSAC was corroborated in the validation cohort, with higher c-index than other models (all P<0.050). Conclusions: On-therapy changes in NFM are an independent indicator of HCC risk. FSAC incorporating NFM response is a reliable risk score for risk estimation for HCC with better performance than other models.
Amelioration of experimental autoimmune encephalomyelitis in Lewis rats treated with fucoidan
Kim, Heechul,Moon, Changjong,Park, Eun-jin,Jee, Youngheun,Ahn, Meejung,Wie, Myung Bok,Shin, Taekyun John Wiley Sons, Ltd. 2010 Phytotherapy research Vol.24 No.3
<P>We examined whether fucoidan affected the clinical symptoms of experimental autoimmune encephalomyelitis (EAE) in rats. EAE was induced in Lewis rats that were immunized with guinea-pig myelin basic protein (MBP) and complete Freund's adjuvant. Fucoidan (50 mg/kg, daily) was administered to rats with EAE intraperitoneally, either in the EAE induction phase from either 1 day before immunization to day 7 post-immunization (PI), or the effector phase from day 8 to 14 PI, to test which phase of rat EAE is affected by fucoidan treatment.</P><P>The onset, severity and duration of EAE paralysis in the fucoidan-treated group in the days 8–14 PI-treated rats, but not in days −1–7 PI-treated rats, were significantly delayed, suppressed and reduced, respectively, compared with the vehicle-treated controls. Treatment with fucoidan reduced the encephalitogenic response and TNF-&agr; production during EAE. Moreover, the clinical amelioration coincided with decreased infiltration of inflammatory cells in the EAE-affected spinal cord. The ameliorative effect of fucoidan on clinical paralysis in EAE-affected rats may be mediated, in part, by the suppression of the autoreactive T cell response and inflammatory cytokine production. Copyright © 2009 John Wiley & Sons, Ltd.</P>
Kim, Jaeyun,Arifin, Dian R.,Muja, Naser,Kim, Taeho,Gilad, Assaf A.,Kim, Heechul,Arepally, Aravind,Hyeon, Taeghwan,Bulte, Jeff W. M. WILEY‐VCH Verlag 2011 Angewandte Chemie Vol.123 No.10
<P><B><I>Doppeltes Abschirmen</I></B> von Inselzellen und multimodale Bildgebung mithilfe eines Kapsel‐in‐Kapsel‐Systems werden in der Zuschrift von T. Hyeon et al. auf S. 2365 ff. beschrieben. Die semipermeable äußere Alginatmembran unterbindet das Eindringen von Immunzellen und Antikörpern, lässt aber die ungehinderte Diffusion von Nährstoffen, Glucose, Sauerstoff und von den Inselzellen produziertem Insulin zu. Die innere Kapsel, die Eisenoxid‐ und Gold‐Bildgebungsagentien enthält, schützt die Zellen vor dem direkten Kontakt mit den Nanopartikeln.</P>