원산지별 감초추출물의 항산화활성 증가를 위한 효율적인 추출조건 탐색

하지훈 ( Ji Hoon Ha ),이혜미 ( Hye Mi Lee ),권순식 ( Soon Sik Kwon ),김해수 ( Hae Soo Kim ),김문진 ( Moon Jin Kim ),전소하 ( So Ha Jeon ),정유민 ( Yoo Min Jeong ),황준필 ( Jun Pil Hwang ),박종호 ( Jong-ho Park ),최영기 ( Yung-key 대한화장품학회 2013 대한화장품학회지 Vol.39 No.4

본 연구에서는 한국 및 중국산 감초(Glycyrrhiza uralensis), 그리고 우즈베키스탄산 감초(Glycyrrhiza glabra)를 대상으로 추출용매, 추출온도, 추출시간 등 추출조건별 추출물을 제조하고 이들 추출물들의 추출 수율과 항산화 활성을 비교하여 최적의 추출조건을 선정하였다. 항산화 활성 중 자유 라디칼(1,1-phenyl-2-pic-rylhydrazyl, DPPH) 소거활성은 85% 에탄올로 60 ℃에서 6 h 동안 추출한 조건에서 한국 감초로부터 얻은 추출물이 가장 높은 활성(46.05%)을 나타내었다. 루미놀 발광법을 이용한 추출물들의 총 항산화능 측정 실험과 피부 광노화에 중요한 ¹O₂으로 유도된 세포막 손상에 대한 추출물들의 세포 보호 효과를 측정한 실험 모두에서도 위의 조건에서 가장 높은 항산화 활성을 나타내었다. 특히, 한국 감초는 τ₅₀이 116.4 min으로 비교 물질인(+)-α-tocopherol (28.5 min)보다 약 4 배나 높은 세포 보호 효과를 나타내었으며, 추출 수율은 18.75%로 우즈베키스탄 및 중국 감초보다 각각 1.2 배 및 2.5 배의 추출 수율을 나타내었다. 따라서, 본 연구 결과는 항산화 소재로 화장품에 응용하기 위하여 감초로부터 추출물을 얻기 위한 최적의 조건은 85% 에탄올로 60 ℃에서 6시간 동안 추출하는 것임을 보여주었다. In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent, temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra (G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidative activities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scav-enging activity (46.05%) under the extraction condition of 85% ethanol at 60 ℃ for 6 hours. The prominent ROS (reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protective effect against ¹O₂ induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (τ₅₀ = 116.4 min) than (+)-α-tocopherol (τ₅₀ = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2 times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extraction condition of 85% ethanol at 60 ℃ for 6 hours is optimal to prepare licorice extracts, which can be applicable as antioxidative cosmetic materials.

유치원 원장 자기평가 척도 개발을 위한 기초연구

신민경,황해익 부산유아교육학회 2004 유아교육논총 Vol.13 No.-

본 연구는 유치원 원장의 자기평가척도 시안을 제작하여 적절성을 알아보고자 부산·울산지역의 공, 사립 유치원 원장 162명을 대상으로 하여 설문조사를 실시하였다. 본 연구의 연구내용은 첫째, 문헌고찰을 통해 유치원 원장자기평가척도 시안을 개발한다. 둘째, 개발한 유치원 원장자기평가척도 시안의 적절성을 알아본다로 설정하였다. 문헌연구와 전문가 협의 및 예비 조사를 통해 3개 영역, 9개 부문, 65문항의 유치원 원장자기평가척도 시안이 개발되었다. 둘째, 유치원 원장자기평가척도 시안의 적절성을 알아본 결과 문항양호도와 척도의 신뢰도·타당도는 양호한 것으로 나타났다. The purpose of this study was to provide preliminary data in order to development a kindergarten principal self-evaluation rating scale. The subjects of this study were selected 162 principals from public and private kindergarten in Busan and Ulsan city. The research contents were as follows: First, the prototype version of kindergarten principal self-evaluation rating scale was developed. Second, the adequacy of the prototype version of kindergarten principal self-evaluation rating scale(the adequacy of the items and scale) were analyzed. The collected data was analyzed by SPSS PC^(+) 10.0 version program. The results of this study were as follows: First, the scale consisted of 65 items in 9 sub-areas of 3 major areas with 5-point rating. The major areas was general, professional, and administration characteristic. The general characteristic area are 30 questionnarie including 4 sub-areas of physical, personal character, leadership, intellectual ability. The professional characteristic area are 26 questionnarie including 4 sub-areas of attitude about profession, professional knowledge. supervision ability. The administration characteristic area are 9 questionnarie including 2 sub-areas of administration practice ability, safe management ability. Second, as the result of adequacy of the items, response distribution on each items and means, standard deviation was goodness. And as the result of t-test, between the higher 27% and lower 27% of self-evaluation score, the all items were significantly discriminated. As the result of adequacy of the scale, internal consistency reliability was .84~.91 in the sub-areas and .97 in the total scale. The construct validity was .72~.91, as correlation between the total rating score and each sub-area's rating score.

電壓形 PWM Inverter에 依한 誘導電動機의 精密 速度制御

金玟會,鄭瓊採,金東熙,李達海,黃敦夏 嶺南大學校 工業技術硏究所 1992 연구보고 Vol.20 No.1

For precision control of Induction Motor with Voltage Source PWM Inverter, the current component should be converted into the voltage one. We are in difficulties for precision control with simple current feedback control because of the oscillation in system and torque ripple on high speed rotation and transient switching resulted from the disturbance which is generated by the mutual interference between d-axis and q-axis during conversion. This paper propose linearized Decoupling Control Theory in Field-Oriented Control and presents controllable system with Microcomputer software in the whole control system. In addition, by experiment it is proved that the system is controlled well in both steady and transient states. The proposed system is expected to be applied in industrial AC Servo System.

유아교사의 과학활동에 대한 수행장애 요인의 정도에 따른 수행수준 연구

유민임,황해익 부산유아교육학회 2003 유아교육논총 Vol.11 No.-

본 연구의 목적은 유아교육현장 교사들의 과학활동에 대한 수행장애요인과 그에 따른 수행 수준과의 관계를 알아보는 것이었다. 연구대상은 공·사립유치원 교사 184명이었다. 연구결과 교사들이 과학활동을 하는데 가장 큰 장애요인은 현직연수나 워크샵의 부족, 그 다음으로 교재교구와 장비의 부족을 들었다. 가학활동에 있어 수행장애요인에 따른 수행수준에서 과학활동에 대한 전문지식과 자신감 부족, 교사들의 영향으로 과학활동을 하는데 방해받는다는 항목에서는 유의한 차이가 있었다. The purpose of this study is to analyze effects of kindergarten teachers’ impediments on their level of science activity. The following research questions are tested: First, What are the trends in kindergarten teachers’ impediments of science activity? Second, What are the trends in kindergarten teachers’ level of science activity? Third, Are there any differences in teachers’ level of science activity based on the impediments in teaching science? The findings of this study are as follows. First, More than fifty percent teachers report that all factors except their colleagues’ interference impede on teaching science. The lack of workshops and seminars appears to be the highest score among impediments. A deficit of materials and instruments is the following impediment teachers indicated. Second, The outcome shows that the mean score of overall level of teachers’ activity is 3.29(SD=.43) which consists of the third level(formal and mechanical). Third, There is a significant association between the level of activity and impediments, especially lacks of special knowledge and self-efficacy and their colleagues’ interference. Teachers who show lower scores in the lack of special knowledge and self-efficacy(F=5.42, p<.05) and their colleagues’ interference(F=10.71, p<.001) maintain higher level of science activity than teachers of higher scores.

韓國人에서 HumFIBRA/FGA와 D21S11 遺傳座의 對立遺傳子 頻度와 遺傳的 變異의 分析

김윤신,황적준,이혜린,구태완,한길로,김성민,이혜승 大韓法醫學會 1998 대한법의학회지 Vol.22 No.1

Allele-and genotype frequencies of the two short tandem repeat (STR) loci, HumFGA and D21S11, were determined in Korean population(n=196). DNA typing was accomplished by applying fluorescence-labeled PCR products and a differently labeled sequenced allelic ladders, followed by automated analysis using ABI 377 automatic sequencer and GeneScan 2.02 software. Prior to typing, allelic ladder of each locus was constructed with a combination of all alleles occuring from the population sample. A total of 15 alleles and 48 genotypes with the heterozygosity of 0.854 for HumFGA, and 12 alleles and 33 genotypes with the heterozygosity of 0.787 for D21S11 are observed in a population of 196 genetically unrelated individuals. No deviations from Hardy-Weinberg equilibrium were observed(p=0.753 for HumFGA, p=0.262 for D21S11). The data presented here (power of discrimination and average power of exclusion) show that both STR Loci, HumFGA and D21S11, are very informative for individualization from criminal evidences, and are also useful for parentage testing.

한국인에서 다변성 D12S391 유전좌의 집단유전학적 연구

이용욱,김성민,구태완,이혜린,강일호,한길로,이혜승,황적준 大韓法醫學會 1999 대한법의학회지 Vol.23 No.1

The hypervariable short tandem repeat(STR) locus D12S391 was investigated in a Korean population. A total of 14 alleles were detected by size under denaturing conditions in 517 unrelated individuals. To confirm all of the alleles detected in a Korean population, a total of 34 fragments were sequenced. Prior to allele designation, we constructed the allelic ladders containing 11 alleles sequenced in this study. Allele 18 is the most common with a frequency of 0.281 in Koreans, and one variant allele 19.3 which have been confirmed by sequencing, was detected. The observed heterozygosity, the power of discrimination (PD), and the mean exclusion chance (MEC) for the locus D12S392 is 0.781, 0.946 and 0.652 , respectively. No deviation from Hardy-Weinberg equilibrium was observed in a Korean population (p=0.557). In the 424 meioses in 105 Korean families confirmed using other 17 STR loci, no mutation was detected in locus D12S391. The STR locus D12S391system is useful both for the analysis identification and parternity.

속도추정에 의한 유도전동기의 벡터제어에 관한 연구

김동희,정경채,김민희,황돈하,이달해 嶺南大學校 工業技術硏究所 1994 工業技術硏究所論文集 Vol.22 No.1

Recently, the vector controlled induction motors are widely used in the industrial AC servo system requiring high accuracy and fast response. However, in the conventional vector control, by using of speed sensors for speed information of the motor, the system design has been restricted. This paper proposes te vector control system based on speed estimation controls motor speed by estimating angular speed of secondary flux linkages from voltage equations of induction motor without speed and voltage sensor. To stabilize the control system for estimation, the ??-axis flux keeps to zero by the compensation control method with state feedback. In addition, the whole control system is designed to be controlled by software in microcomputer. Computer simulations domonstrates that the control characteristics of the proposed vector control system based on speed estimation are satisfactory in both dynamic performance and accuracy.

Dietary intake assessment of macro, trace, and toxic elements via consumption of kimchi in South Korea

Hwang, In Min,Yang, Ji‐,Su,Jung, Ji‐,Hye,Lee, Hae‐,Won,Lee, Hee Min,Seo, Hye‐,Young,Khan, Naeem,Jamila, Nargis,Kim, Kyong Su,Kim, Sung Hyun John Wiley & Sons 2019 Journal of the Science of Food and Agriculture Vol.99 No.14

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>Kimchi, a Korean food, is now produced and consumed in many other countries. In this study, the macro, trace, and toxic element content in varieties of kimchi from Korea and China was evaluated. A health‐risk assessment, involving the calculation of estimated dietary intake values, was undertaken. To discriminate among samples with different origins, linear discriminant analysis (LDA) and principal component analysis (PCA) were applied.</P><P><B>RESULTS</B></P><P>The analytical methods used for analyses were successfully validated. In the quantification of elements, no significant differences were detected in the concentrations of the elements that were analyzed. Based on the fresh weight of kimchi, among the macro elements, the concentrations of Na and K were relatively high, with values of 4181.9–9919.8 mg kg<SUP>−1</SUP> and 1661.8–6623.5 mg kg<SUP>−1</SUP>, respectively. Among the trace elements that were analyzed, zinc in Korean samples, and strontium in Chinese samples showed the highest concentrations, at 2.67 and 2.98 mg kg<SUP>−1</SUP>, respectively. Concentration levels of the toxic trace elements were within permissible limits. The amount of daily intake and provisional daily intake of each element calculated based on kimchi intake confirmed its safety for consumption. Linear discriminant analysis and PCA successfully discriminated among the samples of Chinese and Korean origin.</P><P><B>CONCLUSION</B></P><P>The provisional daily intake of the toxic elements was within provisional tolerable daily intake values. The normal intake of kimchi could not pose a threat upon consumption. © 2019 Society of Chemical Industry</P>

Overexpression of Insulin Degrading Enzyme could Greatly Contribute to Insulin Down-regulation Induced by Short-Term Swimming Exercise

Min Sun Kim,Jun Seo Goo,Ji Eun Kim,So Hee Nam,Sun Il Choi,Hye Ryun Lee,In Sik Hwang,Sun Bo Shim,Seung Wan Jee,Su Hae Lee,Chang Joon Bae,Jung Sik Cho,Jun Yong Cho,Dae Youn Hwang 한국실험동물학회 2011 Laboratory Animal Research Vol.27 No.1

Exercise training is highly correlated with the reduced glucose-stimulated insulin secretion (GSIS), although it enhanced insulin sensitivity, glucose uptake and glucose transporter expression to reduce severity of diabetic symptoms. This study investigated the impact of short-term swimming exercise on insulin regulation in the Goto-Kakizaki (GK) rat as a non-obese model of non-insulin-dependent diabetes mellitus. Wistar (W/S) and GK rats were trained 2 hours daily with the swimming exercise for 4 weeks, and then the changes in the metabolism of insulin and glucose were assessed. Body weight was markedly decreased in the exercised GK rats compare to their non-exercised counterpart, while W/S rats did not show any exercise-related changes. Glucose concentration was not changed by exercise, although impaired glucose tolerance was improved in GK rats 120 min after glucose injection. However, insulin concentration was decreased by swimming exercise as in the decrease of GSIS after running exercise. To identify the other cause for exercise-induced insulin down-regulation, the changes in the levels of key factors involved in insulin production (C-peptide) and clearance (insulin-degrading enzyme; IDE) were measured in W/S and GK rats. The C-peptide level was maintained while IDE expression increased markedly. Therefore, these results showed that insulin down-regulation induced by short-term swimming exercise likely attributes to enhanced insulin clearance via IDE over-expression than by altered insulin production.

Breeding of Table Grape ‘Hongaram’ with Muscat Flavor

Hae Sung Hwang(황해성),Sung Min Jung(정성민),Seo Jun Park(박서준),Youn Young Hur(허윤영),Hae Keun Yun(윤해근),Kyo Sun Park(박교선),Jung Ho Noh(노정호) 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.10